MytuD.18255.a
L,D-transpeptidase
| CENTER ID: | MytuD.18255.a |
| ORGANISM: | Mycobacterium tuberculosis H37Rv |
| ASSOCIATED DISEASE: | Tuberculosis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIC |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MytuD.18255.a.A2.GE34211 | mature protein | 20 | 271 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Structures
4K73
DEPOSITED: 4/16/2013
DETERMINATION: XRay
CLONE: MytuD.18255.a.A2.GE34211
PROTEIN: MytuD.18255.a.A2.PS01609
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| BV-BRC: | fig|83332.12.peg.1598 |
| RefSeq: | NP_215949 |
| TubercuList: | Rv1433 |
| MTB Network Portal: | Rv1433 |
| UniProt: | O06825 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRAVFGCAIA VVGIAGSVVA GPADIHLVAA KQSYGFAVAS VLPTRGQVVG VAHPVVVTFS APITNPANRH AAERAVEVKS TPAMTGKFEW LDNDVVQWVP DRFWPAHSTV ELSVGSLSSD FKTGPAVVGV ASISQHTFTV SIDGVEEGPP PPLPAPHHRV HFGEDGVMPA SMGRPEYPTP VGSYTVLSKE RSVIMDSSSV GIPVDDPDGY RLSVDYAVRI TSRGLYVHSA PWALPALGLE NVSHGCISLS REDAEWYYNA VDIGDPVIVQ E
NT Sequence
atgcgggcgg tttttgggtg tgctattgcc gtcgtcggga tcgctgggag cgtggttgcg gggccggccg acatacacct ggtggcggcg aagcagtctt acgggttcgc cgtcgcgtcg gtgctaccaa cgcgcggcca ggtggtgggc gtggcgcacc ccgtggtggt gacgttcagt gcgccgataa ctaacccagc caatcggcac gcggccgagc gcgccgttga agtcaaatcg acgcccgcga tgaccggcaa gttcgaatgg ctcgacaacg acgttgtgca gtgggttccc gaccgcttct ggccggcgca cagcacggtg gagctttcgg tgggcagcct gtcgagcgat ttcaagacgg gtcccgccgt cgtcggggtt gccagcatct cccagcacac gttcaccgtg agtatcgacg gagtcgagga gggaccgccg cctccgctgc cggcgccgca ccaccgagtg cacttcggcg aagatggggt gatgccggca tcgatgggta gaccggaata cccgacgccg gtcggctcct acactgtctt gtccaaggaa cgctcggtga ttatggattc gagcagcgtc ggcatccccg tcgacgatcc cgatggttac cggctttcgg tggattatgc cgtccgcatc accagccgcg gcctctacgt gcattcagcc ccgtgggccc ttccagcact gggacttgaa aatgtcagcc acggctgcat aagcctgagc cgcgaggacg cagagtggta ttacaacgcg gtcgacattg gcgacccggt cattgtgcag gaatag
Details for MytuD.18255.a.A2.GE34211
| HARVESTED ON: | 12/19/2011 |
| SEQUENCED ON: | 11/13/2014 |
| EXPECTED MW: | 29kDa |
| OBSERVED MW: | 29kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | Low Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMAGPADIHL VAAKQSYGFA VASVLPTRGQ VVGVAHPVVV TFSAPITNPA NRHAAERAVE VKSTPAMTGK FEWLDNDVVQ WVPDRFWPAH STVELSVGSL SSDFKTGPAV VGVASISQHT FTVSIDGVEE GPPPPLPAPH HRVHFGEDGV MPASMGRPEY PTPVGSYTVL SKERSVIMDS SSVGIPVDDP DGYRLSVDYA VRITSRGLYV HSAPWALPAL GLENVSHGCI SLSREDAEWY YNAVDIGDPV IVXGII
Validated NT Sequence
gtttattatt ccnngcacaa tgaccgggtc gccaatgtcg accgcgttgt aataccactc tgcgtcctcg cggctcaggc ttatgcagcc gtggctgaca ttttcaagtc ccagtgctgg aagggcccac ggggctgaat gcacgtagag gccgcggctg gtgatgcgga cggcataatc caccgaaagc cggtaaccat cgggatcgtc gacggggatg ccgacgctgc tcgaatccat aatcaccgag cgttccttgg acaagacagt gtaggagccg accggcgtcg ggtattccgg tctacccatc gatgccggca tcaccccatc ttcgccgaag tgcactcggt ggtgcggcgc cggcagcgga ggcggcggtc cctcctcgac tccgtcgata ctcacggtga acgtgtgctg ggagatgctg gcaaccccga cgacggcggg acccgtcttg aaatcgctcg acaggctgcc caccgaaagc tccaccgtgc tgtgcgccgg ccagaagcgg tcgggaaccc actgcacaac gtcgttgtcg agccattcga acttgccggt catcgcgggc gtcgatttga cttcaacggc gcgctcggcc gcgtgccgat tggctgggtt agttatcggc gcactgaacg tcaccaccac ggggtgcgcc acgcccacca cctggccgcg cgttggtagc accgacgcga cggcgaaccc gtaagactgc ttcgccgcca ccaggtgtat gtcggccggc cccgccatcg aaccaggacc ctgggtctga gcttccaggg tacccatatg atggtgatgg tgatgagcca t
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAGPADIHL VAAKQSYGFA VASVLPTRGQ VVGVAHPVVV TFSAPITNPA NRHAAERAVE VKSTPAMTGK FEWLDNDVVQ WVPDRFWPAH STVELSVGSL SSDFKTGPAV VGVASISQHT FTVSIDGVEE GPPPPLPAPH HRVHFGEDGV MPASMGRPEY PTPVGSYTVL SKERSVIMDS SSVGIPVDDP DGYRLSVDYA VRITSRGLYV HSAPWALPAL GLENVSHGCI SLSREDAEWY YNAVDIGDPV IVQE
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcggggccg gccgacatac acctggtggc ggcgaagcag tcttacgggt tcgccgtcgc gtcggtgcta ccaacgcgcg gccaggtggt gggcgtggcg caccccgtgg tggtgacgtt cagtgcgccg ataactaacc cagccaatcg gcacgcggcc gagcgcgccg ttgaagtcaa atcgacgccc gcgatgaccg gcaagttcga atggctcgac aacgacgttg tgcagtgggt tcccgaccgc ttctggccgg cgcacagcac ggtggagctt tcggtgggca gcctgtcgag cgatttcaag acgggtcccg ccgtcgtcgg ggttgccagc atctcccagc acacgttcac cgtgagtatc gacggagtcg aggagggacc gccgcctccg ctgccggcgc cgcaccaccg agtgcacttc ggcgaagatg gggtgatgcc ggcatcgatg ggtagaccgg aatacccgac gccggtcggc tcctacactg tcttgtccaa ggaacgctcg gtgattatgg attcgagcag cgtcggcatc cccgtcgacg atcccgatgg ttaccggctt tcggtggatt atgccgtccg catcaccagc cgcggcctct acgtgcattc agccccgtgg gcccttccag cactgggact tgaaaatgtc agccacggct gcataagcct gagccgcgag gacgcagagt ggtattacaa cgcggtcgac attggcgacc cggtcattgt gcaggaataa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa