MyleA.18255.a

POSSIBLE CONSERVED MEMBRANE PROTEIN

CENTER ID: MyleA.18255.a
ORGANISM: Mycobacterium leprae Br4923
ASSOCIATED DISEASE: Hansen's disease, leprosy
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyleA.18255.a.A2.GE34369 mature protein 31 271
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyleA.18255.a.A2.PS01436 mature protein 31 271
External Resources
RESOURCE REFERENCE ID
OrthoMCL: OG5_237594
PATRIC ID: fig|561304.4.peg.994
RefSeq: YP_002503112.1
UniProt: A0A0H3MYI3
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MGIMRAAIRG VLVVIGIAAT VIAGPVRVSL AAVNHAHGFA ITSILPTRDA VVGVAHPVVV TFGAPVTNRK ATELSLKVRS APAMNGKFDW LDSKVVQWVP DRYWPEHSTI ALAVGNLSTN FKTGPAILGI ADISNHTFTV TIDGVEAETP PPLPSPHHRP HWGEEGVMPA SMGKTEFPTP TGKYTVMSKD RSVIMDSSSV GIPVDDPEGY RLSVDYAVRI SSRGLYIHSA PWAVQSMGFD NTSHGCISLS PADAEWYYNT VNIGDPVIVK E
NT Sequence
atgggaatta tgcgggcagc aattcgaggt gttcttgtgg tgatcgggat cgcggcgacg gtgatcgcgg gaccggtgcg tgtaagccta gcggcggtga accatgcgca tggcttcgcc atcacgtcaa ttctacctac acgggacgcg gtggtgggtg tggcgcaccc cgtggtagtg acattcggcg cacccgtcac taaccgtaag gcgaccgagc tttcccttaa agtgcgctcg gcgccagcga tgaacggcaa gtttgactgg ctcgacagca aagttgtgca gtgggttcct gaccgctatt ggccagagca cagcacgatc gcactcgcgg tgggcaacct gtccaccaac ttcaaaacgg gtcctgccat tctcggtatt gcggatatct cgaatcacac gttcacggtg acgatcgacg gagtcgaggc ggagacaccc cctccactgc cttcacccca ccatcggccc cactggggag aagaaggggt gatgccggcc tcgatgggta agacagaatt cccgacacct accggtaagt ataccgtcat gtccaaggat cgctcggtga ttatggattc gagtagtgtc ggcatacctg tcgacgatcc cgaggggtac aggctctcgg tggattacgc cgtccgtatc agcagccgtg gcctctacat ccattcggct ccgtgggcgg tgcagtcaat gggttttgat aacaccagcc acggctgcat tagcttaagc cctgccgacg cagaatggta ctacaacaca gttaacattg gtgacccggt aatcgtgaag gaataa
Details for MyleA.18255.a.A2.GE34369
HARVESTED ON: 1/10/2012
SEQUENCED ON: 1/13/2012
EXPECTED MW: 28kDa
OBSERVED MW: 34kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMAAVNHAHG FAITSILPTR DAVVGVAHPV VVTFGAPVTN RKATELSLKV RSAPAMNGKF DWLDSKVVQW VPDRYWPEHS TIALAVGNLS TNFKTGPAIL GIADISNHTF TVTIDGVEAE TPPPLPSPHH RPHWGEEGVM PASMGKTEFP TPTGKYTVMS KDRSVIMDSS SVGIPVDDPE GYRLSVDYAV RISSRGLYIH SAPWAVQSMG FDNTSHGCIS LSPADAEWYY NTVNIGDPVI V
Validated NT Sequence
tcacgattac cgggtcacca atgttaactg tgttgtagta ccattctgcg tcggcagggc ttaagctaat gcagccgtgg ctggtgttat caaaacccat tgactgcacc gcccacggag ccgaatggat gtagaggcca cggctgctga tacggacggc gtaatccacc gagagcctgt acccctcggg atcgtcgaca ggtatgccga cactactcga atccataatc accgagcgat ccttggacat gacggtatac ttaccggtag gtgtcgggaa ttctgtctta cccatcgagg ccggcatcac cccttcttct ccccagtggg gccgatggtg gggtgaaggc agtggagggg gtgtctccgc ctcgactccg tcgatcgtca ccgtgaacgt gtgattcgag atatccgcaa taccgagaat ggcaggaccc gttttgaagt tggtggacag gttgcccacc gcgagtgcga tcgtgctgtg ctctggccaa tagcggtcag gaacccactg cacaactttg ctgtcgagcc agtcaaactt gccgttcatc gctggcgccg agcgcacttt aagggaaagc tcggtcgcct tacggttagt gacgggtgcg ccgaatgtca ctaccacggg gtgcgccaca cccaccaccg cgtcccgtgt aggtagaatt gacgtgatgg cgaagccatg cgcatggttc accgccgcca tcgaaccagg accctgggtc tgagcttcca gggtacccat atgatggtga tggtgatgag ccatggtata tctccttctt aa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAAVNHAHG FAITSILPTR DAVVGVAHPV VVTFGAPVTN RKATELSLKV RSAPAMNGKF DWLDSKVVQW VPDRYWPEHS TIALAVGNLS TNFKTGPAIL GIADISNHTF TVTIDGVEAE TPPPLPSPHH RPHWGEEGVM PASMGKTEFP TPTGKYTVMS KDRSVIMDSS SVGIPVDDPE GYRLSVDYAV RISSRGLYIH SAPWAVQSMG FDNTSHGCIS LSPADAEWYY NTVNIGDPVI VKE
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcggcggtg aaccatgcgc atggcttcgc catcacgtca attctaccta cacgggacgc ggtggtgggt gtggcgcacc ccgtggtagt gacattcggc gcacccgtca ctaaccgtaa ggcgaccgag ctttccctta aagtgcgctc ggcgccagcg atgaacggca agtttgactg gctcgacagc aaagttgtgc agtgggttcc tgaccgctat tggccagagc acagcacgat cgcactcgcg gtgggcaacc tgtccaccaa cttcaaaacg ggtcctgcca ttctcggtat tgcggatatc tcgaatcaca cgttcacggt gacgatcgac ggagtcgagg cggagacacc ccctccactg ccttcacccc accatcggcc ccactgggga gaagaagggg tgatgccggc ctcgatgggt aagacagaat tcccgacacc taccggtaag tataccgtca tgtccaagga tcgctcggtg attatggatt cgagtagtgt cggcatacct gtcgacgatc ccgaggggta caggctctcg gtggattacg ccgtccgtat cagcagccgt ggcctctaca tccattcggc tccgtgggcg gtgcagtcaa tgggttttga taacaccagc cacggctgca ttagcttaag ccctgccgac gcagaatggt actacaacac agttaacatt ggtgacccgg taatcgtgaa ggaataaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa
Details for MyleA.18255.a.A2.PS01436
PURIFICATION DATe: 3/29/2012
CONCENTRATION: 18.9mg/ml
OBSERVED MW: 29kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 6
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMAAVNHAHG FAITSILPTR DAVVGVAHPV VVTFGAPVTN RKATELSLKV RSAPAMNGKF DWLDSKVVQW VPDRYWPEHS TIALAVGNLS TNFKTGPAIL GIADISNHTF TVTIDGVEAE TPPPLPSPHH RPHWGEEGVM PASMGKTEFP TPTGKYTVMS KDRSVIMDSS SVGIPVDDPE GYRLSVDYAV RISSRGLYIH SAPWAVQSMG FDNTSHGCIS LSPADAEWYY NTVNIGDPVI V
Validated NT Sequence
tcacgattac cgggtcacca atgttaactg tgttgtagta ccattctgcg tcggcagggc ttaagctaat gcagccgtgg ctggtgttat caaaacccat tgactgcacc gcccacggag ccgaatggat gtagaggcca cggctgctga tacggacggc gtaatccacc gagagcctgt acccctcggg atcgtcgaca ggtatgccga cactactcga atccataatc accgagcgat ccttggacat gacggtatac ttaccggtag gtgtcgggaa ttctgtctta cccatcgagg ccggcatcac cccttcttct ccccagtggg gccgatggtg gggtgaaggc agtggagggg gtgtctccgc ctcgactccg tcgatcgtca ccgtgaacgt gtgattcgag atatccgcaa taccgagaat ggcaggaccc gttttgaagt tggtggacag gttgcccacc gcgagtgcga tcgtgctgtg ctctggccaa tagcggtcag gaacccactg cacaactttg ctgtcgagcc agtcaaactt gccgttcatc gctggcgccg agcgcacttt aagggaaagc tcggtcgcct tacggttagt gacgggtgcg ccgaatgtca ctaccacggg gtgcgccaca cccaccaccg cgtcccgtgt aggtagaatt gacgtgatgg cgaagccatg cgcatggttc accgccgcca tcgaaccagg accctgggtc tgagcttcca gggtacccat atgatggtga tggtgatgag ccatggtata tctccttctt aa
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAAVNHAHG FAITSILPTR DAVVGVAHPV VVTFGAPVTN RKATELSLKV RSAPAMNGKF DWLDSKVVQW VPDRYWPEHS TIALAVGNLS TNFKTGPAIL GIADISNHTF TVTIDGVEAE TPPPLPSPHH RPHWGEEGVM PASMGKTEFP TPTGKYTVMS KDRSVIMDSS SVGIPVDDPE GYRLSVDYAV RISSRGLYIH SAPWAVQSMG FDNTSHGCIS LSPADAEWYY NTVNIGDPVI VKE
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGCGGCGGTG AACCATGCGC ATGGCTTCGC CATCACGTCA ATTCTACCTA CACGGGACGC GGTGGTGGGT GTGGCGCACC CCGTGGTAGT GACATTCGGC GCACCCGTCA CTAACCGTAA GGCGACCGAG CTTTCCCTTA AAGTGCGCTC GGCGCCAGCG ATGAACGGCA AGTTTGACTG GCTCGACAGC AAAGTTGTGC AGTGGGTTCC TGACCGCTAT TGGCCAGAGC ACAGCACGAT CGCACTCGCG GTGGGCAACC TGTCCACCAA CTTCAAAACG GGTCCTGCCA TTCTCGGTAT TGCGGATATC TCGAATCACA CGTTCACGGT GACGATCGAC GGAGTCGAGG CGGAGACACC CCCTCCACTG CCTTCACCCC ACCATCGGCC CCACTGGGGA GAAGAAGGGG TGATGCCGGC CTCGATGGGT AAGACAGAAT TCCCGACACC TACCGGTAAG TATACCGTCA TGTCCAAGGA TCGCTCGGTG ATTATGGATT CGAGTAGTGT CGGCATACCT GTCGACGATC CCGAGGGGTA CAGGCTCTCG GTGGATTACG CCGTCCGTAT CAGCAGCCGT GGCCTCTACA TCCATTCGGC TCCGTGGGCG GTGCAGTCAA TGGGTTTTGA TAACACCAGC CACGGCTGCA TTAGCTTAAG CCCTGCCGAC GCAGAATGGT ACTACAACAC AGTTAACATT GGTGACCCGG TAATCGTGAA GGAATAAACA GCACGAACAA GTTCTGCAGC CAAGCTTCTC GAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAA