MytuD.18254.a

PROBABLE TRANSALDOLASE TAL

CENTER ID: MytuD.18254.a
ORGANISM: Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE: Tuberculosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MytuD.18254.a.A2.GE34212 mature protein 20 373
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|83332.12.peg.1617
RefSeq: NP_215964
TubercuList: Rv1448c
MTB Network Portal: Rv1448c
UniProt: P9WG33

Orthologues for Rv1448c

Species % Identity % Coverage Clones Proteins Structures
leprae 79 99 1 0 0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTAQNPNLAA LSAAGVSVWL DDLSRDRLRS GNLQELIDTK SVVGVTTNPS IFQKALSEGH TYDAQIAELA ARGADVDATI RTVTTDDVRS ACDVLVPQWE DSDGVDGRVS IEVDPRLAHE TEKTIQQAIE LWKIVDRPNL FIKIPATKAG LPAISAVLAE GISVNVTLIF SVQRYREVMD AYLTGMEKAR QAGHSLSKIH SVASFFVSRV DTEIDKRLDR IGSRQALELR GQAGVANARL AYATYREVFE DSDRYRSLKV DGARVQRPLW ASTGVKNPDY SDTLYVTELV APHTVNTMPE KTIDAVADHG VIQGDTVTGT ASDAQAVFDQ LGAIGIDLTD VFAVLEEEGV RKFEASWNEL LQETRAHLDT AAQ
NT Sequence
atgaccgctc agaaccccaa cctggccgca ttgagcgccg cgggggtatc ggtgtggctg gatgacctgt cgcgggaccg gctgcgctcg ggcaatctgc aggagctgat cgacaccaag agcgtcgtcg gggtgaccac caacccgtcg atctttcaga aggcgctgtc ggagggccac acctacgacg cccagatcgc cgagctggcc gcgcgcggcg ccgacgtgga tgccaccatc cgcaccgtca ccactgacga cgtgcgcagc gcgtgcgacg tgctggtccc ccagtgggag gactccgacg gggtcgacgg ccgggtgtcg atcgaggtcg acccgcggct ggcacacgaa accgaaaaaa cgatccaaca ggcaatcgag ctgtggaaga tcgtcgaccg gccgaacctt ttcatcaaga tcccggccac taaggccggt ctgcccgcca tcagtgccgt tctggcggaa gggatttcgg tcaacgttac gctgatcttc tccgtgcagc ggtaccgcga ggtgatggac gcctacctga ccgggatgga aaaggcccgg caagccggac acagcctgtc caagatccat tcggtggcat cgtttttcgt ctcccgggtg gacaccgaaa tcgacaagcg gctggacaga atcggctcgc ggcaagcgct cgaactgcgc ggccaggccg gtgtcgccaa tgcccgcctg gcctatgcca cctaccggga ggtcttcgaa gacagcgatc gttatcggtc cctcaaggtc gacggcgccc gggtccagcg cccgttgtgg gcttccaccg gcgtgaagaa ccccgattac tcggacaccc tctacgtcac cgagttggtc gccccacaca ccgtgaacac tatgccggaa aagacaattg acgccgtcgc tgatcatggc gtgatccaag gcgacacggt caccggaact gcatccgatg cccaggcggt gttcgaccag ctgggggcga tcggcatcga cctcaccgac gtgtttgccg tcctcgagga ggaaggcgtg cggaagttcg aggcctcctg gaacgagcta ctccaggaaa cccgggcgca tctcgacacc gctgcccaat ga
Details for MytuD.18254.a.A2.GE34212
HARVESTED ON: 12/19/2011
SEQUENCED ON: 12/28/2011
EXPECTED MW: 41kDa
OBSERVED MW: 41kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 83
PERCENT COVERAGE: 97
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMLDDLSRDR LRSGNLQELI DTKSVVGVTT NPSIFQKALS EGHTYDAQIA ELAARGADVD ATIRTVTTDD VRSACDVLVP QWEDSDGVDG RVSIEVDPRL AHETEKTIQQ AIELWKIVDR PNLFIKIPAT KAGLPAISAV LAEGISVNVT LIFSVQRYRE VMDAYLTGME KARQAGHSLS KIHSVASFFV SRVDTEIDKR LDRIGSRQAL ELRGQAGVAN ARLAYATYRE VFEDSDRYRS LKVDGARVXR PLWASTGVKN PDYSDTLYVT XLVAXHTVXT MXKXXXTPSL IXXXSXATXX XXXAXDXXXX DXXGXIXIXX XXXXXSXXXX XXXTXXXXXX XXXXX
Validated NT Sequence
attttnttta ctttaagaag gaganatacc atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgctgg atgacctgtc gcgggaccgg ctgcgctcgg gcaatctgca ggagctgatc gacaccaaga gcgtcgtcgg ggtgaccacc aacccgtcga tctttcagaa ggcgctgtcg gagggccaca cctacgacgc ccagatcgcc gagctggccg cgcgcggcgc cgacgtggat gccaccatcc gcaccgtcac cactgacgac gtgcgcagcg cgtgcgacgt gctggtcccc cagtgggagg actccgacgg ggtcgacggc cgggtgtcga tcgaggtcga cccgcggctg gcacacgaaa ccgaaaaaac gatccaacag gcaatcgagc tgtggaagat cgtcgaccgg ccgaaccttt tcatcaagat cccggccact aaggccggtc tgcccgccat cagtgccgtt ctggcggaag ggatttcggt caacgttacg ctgatcttct ccgtgcagcg gtaccgcgag gtgatggacg cctacctgac cgggatggaa aaggcccggc aagccggaca cagcctgtcc aagatccatt cggtggcatc gtttttcgtc tcccgggtgg acaccgaaat cgacaagcgg ctggacagaa tcggctcgcg gcaagcgctc gaactgcgcg gccaggccgg tgtcgccaat gcccgcctgg cctatgccac ctaccgggag gtcttcgaag acagcgatcg ttatcggtcc ctcaaggtcg acggcgcccg ggtccngcgc ccgttgtggg cttccaccgg cgtgaagaac cccgattact cggacaccct ctacgtcacc nagttggtcg cccnacacac cgtgannact atgnggaaan nacnantgac gccgtcgctg atcntggngn natccnnggc gacncngncn ncngnanntg catncgatnn nnnnnnnntn gacnnctngg gggngatcnn natcnnnnnn nnncnnnnnn nnncntcnan nnnnnnnnnn nnnnnnnnta cnncngnnnn ngnnnntnnn nnnannnnnn nannaa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMLDDLSRDR LRSGNLQELI DTKSVVGVTT NPSIFQKALS EGHTYDAQIA ELAARGADVD ATIRTVTTDD VRSACDVLVP QWEDSDGVDG RVSIEVDPRL AHETEKTIQQ AIELWKIVDR PNLFIKIPAT KAGLPAISAV LAEGISVNVT LIFSVQRYRE VMDAYLTGME KARQAGHSLS KIHSVASFFV SRVDTEIDKR LDRIGSRQAL ELRGQAGVAN ARLAYATYRE VFEDSDRYRS LKVDGARVQR PLWASTGVKN PDYSDTLYVT ELVAPHTVNT MPEKTIDAVA DHGVIQGDTV TGTASDAQAV FDQLGAIGID LTDVFAVLEE EGVRKFEASW NELLQETRAH LDTAAQ
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gctggatgac ctgtcgcggg accggctgcg ctcgggcaat ctgcaggagc tgatcgacac caagagcgtc gtcggggtga ccaccaaccc gtcgatcttt cagaaggcgc tgtcggaggg ccacacctac gacgcccaga tcgccgagct ggccgcgcgc ggcgccgacg tggatgccac catccgcacc gtcaccactg acgacgtgcg cagcgcgtgc gacgtgctgg tcccccagtg ggaggactcc gacggggtcg acggccgggt gtcgatcgag gtcgacccgc ggctggcaca cgaaaccgaa aaaacgatcc aacaggcaat cgagctgtgg aagatcgtcg accggccgaa ccttttcatc aagatcccgg ccactaaggc cggtctgccc gccatcagtg ccgttctggc ggaagggatt tcggtcaacg ttacgctgat cttctccgtg cagcggtacc gcgaggtgat ggacgcctac ctgaccggga tggaaaaggc ccggcaagcc ggacacagcc tgtccaagat ccattcggtg gcatcgtttt tcgtctcccg ggtggacacc gaaatcgaca agcggctgga cagaatcggc tcgcggcaag cgctcgaact gcgcggccag gccggtgtcg ccaatgcccg cctggcctat gccacctacc gggaggtctt cgaagacagc gatcgttatc ggtccctcaa ggtcgacggc gcccgggtcc agcgcccgtt gtgggcttcc accggcgtga agaaccccga ttactcggac accctctacg tcaccgagtt ggtcgcccca cacaccgtga acactatgcc ggaaaagaca attgacgccg tcgctgatca tggcgtgatc caaggcgaca cggtcaccgg aactgcatcc gatgcccagg cggtgttcga ccagctgggg gcgatcggca tcgacctcac cgacgtgttt gccgtcctcg aggaggaagg cgtgcggaag ttcgaggcct cctggaacga gctactccag gaaacccggg cgcatctcga caccgctgcc caataaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa