MyleA.18254.a

PROBABLE TRANSALDOLASE TAL

CENTER ID: MyleA.18254.a
ORGANISM: Mycobacterium leprae Br4923
ASSOCIATED DISEASE: Hansen's disease, leprosy
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyleA.18254.a.A2.GE34370 mature protein 22 375
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|561304.4.peg.1014
RefSeq: YP_002503124.1
UniProt: B8ZUQ2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTSKIQNPNL AALSAAGVSV WLDDLSRDRL QSGNLQKLID TKSVVGVTTN PSIFQKAFAN GHAYDAQIAE LAKRGANVDV TVRTVTTDDV RHACDVLACE WEASHGKDGR VSIEVDPRLA HDTDKTIAQA VELWRIVDHP NLFIKIPATK AGLPAITAVL AEGISVNVTL IFSVQRHREV IDAYLAGIEK AAEAGRDLSK IVSVASFFVS RVDTEIDKRL EKLGSEQALA LRGQAGVANA RLAYAAYQKA FEGGQRYQTL MARGAQVQRP LWASTGVKNP DYADTLYVTE LVAPNTVNTM PETTIDAVAD HGVIRGDTIS GTTLSSQKVF DSLVAVGVDL IDVFAVLEHE GVQKFVKSWN ELLKETQEQL DSVAK
NT Sequence
atgacctcaa aaattcagaa ccccaacctc gccgcattga gcgctgccgg agtgtccgtt tggttggacg acttgtcgcg agaccggttg cagtcaggca acctacagaa gctgattgac accaagagtg tcgttggcgt gaccaccaac ccgtccatct tccagaaagc attcgcaaac ggccacgcct acgatgccca aattgccgag ctggccaagc gcggcgccaa cgtagatgtc accgtccgca ccgtcaccac tgacgatgtt cgccacgcat gcgacgtgtt ggcgtgtgaa tgggaagctt cccacggaaa agatggccga gtctcgattg aagtcgatcc ccgtttagca catgacaccg acaagactat cgcgcaagcc gtcgaactat ggagaatagt tgaccacccg aacttgttca tcaagatccc ggcgactaag gctggcctac cggcgatcac cgccgtttta gcggaaggca tttcggtcaa cgtcacgctg attttctctg tacagcggca tcgcgaggta attgacgcct acctggccgg gatagaaaag gccgccgaag ctggacgcga cctatccaag atagtctcag tagcatcgtt ttttgtctcc cgcgtggaca ccgaaatcga caagcgacta gaaaaacttg gttccgagca agcactcgcg ctacgcggcc aggccggtgt cgccaacgcc cgattggcct acgcggccta ccagaaggcc ttcgaaggtg gccagcgcta ccagacactc atggcccgcg gagcccaagt acagcgcccg ctgtgggcgt caaccggtgt caagaacccc gattacgccg acaccctgta cgtcaccgag ctggtagctc caaacacggt gaacactatg ccggagacga cgattgatgc cgtagccgat cacggtgtga tccgtggtga cacgatcagc ggtactactt tgtcctccca aaaagtgttc gactcactgg tggcggtcgg agtcgatctg attgatgtgt tcgcggtact agaacatgag ggcgtgcaga agtttgtgaa atcctggaac gagctactca aggaaactca ggaacagctg gactccgtcg ccaaatga
Details for MyleA.18254.a.A2.GE34370
HARVESTED ON: 1/10/2012
SEQUENCED ON: 1/13/2012
EXPECTED MW: 40kDa
OBSERVED MW: 40kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 83
PERCENT COVERAGE: 102
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMLDDLSRDR LQSGNLQKLI DTKSVVGVTT NPSIFQKAFA NGHAYDAQIA ELAKRGANVD VTVRTVTTDD VRHACDVLAC EWEASHGKDG RVSIEVDPRL AHDTDKTIAQ AVELWRIVDH PNLFIKIPAT KAGLPAITAV LAEGISVNVT LIFSVQRHRE VIDAYLAGIE KAAEAGRDLS KIVSVASFFV SRVDTEIDKR LEKLGSEQAL ALRGQAGVAN ARLAYAAYQK XFEGGQRYQT LMARGAQVQR PLWASTGVKN PDYADTLYVT ELVAPNTVNT MPXTXXXAXX DHGXIRXTRS XVLLXXPXSX RLLXXXXXSX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXIX XXXXXX
Validated NT Sequence
ttttntttaa ctttaagaag gagatatacc atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgttgg acgacttgtc gcgagaccgg ttgcagtcag gcaacctaca gaagctgatt gacaccaaga gtgtcgttgg cgtgaccacc aacccgtcca tcttccagaa agcattcgca aacggccacg cctacgatgc ccaaattgcc gagctggcca agcgcggcgc caacgtagat gtcaccgtcc gcaccgtcac cactgacgat gttcgccacg catgcgacgt gttggcgtgt gaatgggaag cttcccacgg aaaagatggc cgagtctcga ttgaagtcga tccccgttta gcacatgaca ccgacaagac tatcgcgcaa gccgtcgaac tatggagaat agttgaccac ccgaacttgt tcatcaagat cccggcgact aaggctggcc taccggcgat caccgccgtt ttagcggaag gcatttcggt caacgtcacg ctgattttct ctgtacagcg gcatcgcgag gtaattgacg cctacctggc cgggatagaa aaggccgccg aagctggacg cgacctatcc aagatagtct cagtagcatc gttttttgtc tcccgcgtgg acaccgaaat cgacaagcga ctagaaaaac ttggttccga gcaagcactc gcgctacgcg gccaggccgg tgtcgccaac gcccgattgg cctacgcggc ctaccagaag gncttcgaag gtggccagcg ctaccagaca ctcatggccc gcggagccca agtacagcgc ccgctgtggg cgtcaaccgg tgtcaagaac cccgattacg ccgacaccct gtacgtcacc gagctggtag ctccnaacac ggtgaacact atgccggana cgangannnn tgccntancc gatcacggng ngatccgggn gacncgatca nnngtactac ttngtcntcc nnaaagtnnt cgactcctgg ngnggnngnn tcnatctgnt nnnnnnnnnn ngnactanan ntgnnggcnt gnnnaanttn gngnanncnn nnnnnctann nnnnanngnn tncntcnnnn nnanannnnn nnaannannn nngnannnnn tnnnatcnnn nnnnnnnnnn nnnnnnnnnt
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMLDDLSRDR LQSGNLQKLI DTKSVVGVTT NPSIFQKAFA NGHAYDAQIA ELAKRGANVD VTVRTVTTDD VRHACDVLAC EWEASHGKDG RVSIEVDPRL AHDTDKTIAQ AVELWRIVDH PNLFIKIPAT KAGLPAITAV LAEGISVNVT LIFSVQRHRE VIDAYLAGIE KAAEAGRDLS KIVSVASFFV SRVDTEIDKR LEKLGSEQAL ALRGQAGVAN ARLAYAAYQK AFEGGQRYQT LMARGAQVQR PLWASTGVKN PDYADTLYVT ELVAPNTVNT MPETTIDAVA DHGVIRGDTI SGTTLSSQKV FDSLVAVGVD LIDVFAVLEH EGVQKFVKSW NELLKETQEQ LDSVAK
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gttggacgac ttgtcgcgag accggttgca gtcaggcaac ctacagaagc tgattgacac caagagtgtc gttggcgtga ccaccaaccc gtccatcttc cagaaagcat tcgcaaacgg ccacgcctac gatgcccaaa ttgccgagct ggccaagcgc ggcgccaacg tagatgtcac cgtccgcacc gtcaccactg acgatgttcg ccacgcatgc gacgtgttgg cgtgtgaatg ggaagcttcc cacggaaaag atggccgagt ctcgattgaa gtcgatcccc gtttagcaca tgacaccgac aagactatcg cgcaagccgt cgaactatgg agaatagttg accacccgaa cttgttcatc aagatcccgg cgactaaggc tggcctaccg gcgatcaccg ccgttttagc ggaaggcatt tcggtcaacg tcacgctgat tttctctgta cagcggcatc gcgaggtaat tgacgcctac ctggccggga tagaaaaggc cgccgaagct ggacgcgacc tatccaagat agtctcagta gcatcgtttt ttgtctcccg cgtggacacc gaaatcgaca agcgactaga aaaacttggt tccgagcaag cactcgcgct acgcggccag gccggtgtcg ccaacgcccg attggcctac gcggcctacc agaaggcctt cgaaggtggc cagcgctacc agacactcat ggcccgcgga gcccaagtac agcgcccgct gtgggcgtca accggtgtca agaaccccga ttacgccgac accctgtacg tcaccgagct ggtagctcca aacacggtga acactatgcc ggagacgacg attgatgccg tagccgatca cggtgtgatc cgtggtgaca cgatcagcgg tactactttg tcctcccaaa aagtgttcga ctcactggtg gcggtcggag tcgatctgat tgatgtgttc gcggtactag aacatgaggg cgtgcagaag tttgtgaaat cctggaacga gctactcaag gaaactcagg aacagctgga ctccgtcgcc aaataaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa