MytuD.17946.a
DNA ligase A (Rv3014c)
| CENTER ID: | MytuD.17946.a |
| ORGANISM: | Mycobacterium tuberculosis H37Rv |
| ASSOCIATED DISEASE: | Tuberculosis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIC |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
Materials can be ordered from SSGCID using the button in the "order material" column.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MytuD.17946.a.B1.GE40372 | full length | 1 | 691 | ||
| MytuD.17946.a.B2.GE40367 | domain based on PDB 1ZAU | 1 | 328 | ||
| MytuD.17946.a.B3.GE40368 | domain based on PDB 3SGI | 2 | 605 | ||
| MytuD.17946.a.B4.GE40369 | Domain 4 | 78 | 605 | ||
| MytuD.17946.a.B5.GE40370 | Domain 5 | 78 | 328 | ||
| MytuD.17946.a.B6.GE40371 | Domain 6 | 1 | 417 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MytuD.17946.a.B3.PS38124 | domain based on PDB 3SGI | 2 | 605 | ||
| MytuD.17946.a.B2.PS38127 | domain based on PDB 1ZAU | 1 | 328 | ||
| MytuD.17946.a.B1.PS38422 | full length | 1 | 691 | ||
| MytuD.17946.a.B4.PW38452 | Domain 4 | 78 | 605 |
Structures
7K72
DEPOSITED: 9/22/2020
DETERMINATION: XRay
CLONE: MytuD.17946.a.B4.GE40369
PROTEIN: MytuD.17946.a.B4.PW38452
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| PATRIC ID: | fig|83332.12.peg.3363 |
| RefSeq: | WP_003415263.1 |
| TubercuList: | Rv3014c |
| MTB Network Portal: | Rv3014c |
| UniProt: | P9WNV1 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSSPDADQTA PEVLRQWQAL AEEVREHQFR YYVRDAPIIS DAEFDELLRR LEALEEQHPE LRTPDSPTQL VGGAGFATDF EPVDHLERML SLDNAFTADE LAAWAGRIHA EVGDAAHYLC ELKIDGVALS LVYREGRLTR ASTRGDGRTG EDVTLNARTI ADVPERLTPG DDYPVPEVLE VRGEVFFRLD DFQALNASLV EEGKAPFANP RNSAAGSLRQ KDPAVTARRR LRMICHGLGH VEGFRPATLH QAYLALRAWG LPVSEHTTLA TDLAGVRERI DYWGEHRHEV DHEIDGVVVK VDEVALQRRL GSTSRAPRWA IAYKYPPEEA QTKLLDIRVN VGRTGRITPF AFMTPVKVAG STVGQATLHN ASEIKRKGVL IGDTVVIRKA GDVIPEVLGP VVELRDGSER EFIMPTTCPE CGSPLAPEKE GDADIRCPNA RGCPGQLRER VFHVASRNGL DIEVLGYEAG VALLQAKVIA DEGELFALTE RDLLRTDLFR TKAGELSANG KRLLVNLDKA KAAPLWRVLV ALSIRHVGPT AARALATEFG SLDAIAAAST DQLAAVEGVG PTIAAAVTEW FAVDWHREIV DKWRAAGVRM VDERDESVPR TLAGLTIVVT GSLTGFSRDD AKEAIVARGG KAAGSVSKKT NYVVAGDSPG SKYDKAVELG VPILDEDGFR RLLADGPASR T
NT Sequence
gtgagctccc cagacgccga tcagaccgct cccgaggtgt tgcggcagtg gcaggcactg gccgaggagg tgcgtgagca ccagttccgt tattacgtgc gggacgcgcc gatcatcagc gacgcggaat tcgacgagct gctgcgccgt ctggaagccc tcgaggagca gcatcccgag ctgcgcacgc ccgattcgcc gacccagctg gtcggcggtg ccggcttcgc cacggatttc gagcccgtcg accatctcga acgaatgctc agcctcgaca acgcgttcac cgccgacgaa ctcgccgcct gggccggccg catccatgcc gaggtcggag acgccgcaca ttacctgtgt gagctcaaga tcgacggcgt cgcgctgtct ttggtctacc gcgagggacg gctgacccgg gcctccaccc gcggcgacgg gcgcaccggc gaggacgtca ccctgaacgc ccggaccatc gccgacgttc ccgaacggct cacccccggc gacgactacc cggtgcccga ggtcctcgag gtccgcggcg aggtcttctt ccggctggac gacttccagg cgctcaacgc cagcctcgtc gaggagggca aggcgccgtt cgccaacccc cgcaacagcg cggcgggatc gctgcgccag aaagacccgg cggtcaccgc gcgccgccgg ctgcggatga tctgccacgg gctgggccac gtggagggct ttcgcccggc caccctgcat caggcatacc tggcgttgcg ggcatgggga ctgccggttt ccgaacacac caccctggca accgacctgg ccggtgtgcg cgagcgcatc gactactggg gcgagcaccg ccacgaggtg gaccacgaaa tcgacggcgt ggtggtcaaa gtcgacgagg tggcgttgca gcgcaggctg ggttccacgt cgcgggcgcc gcgctgggcc atcgcctaca agtacccgcc cgaggaagcg cagaccaagc tgctcgacat ccgggtgaac gtcggccgca ccgggcggat cacgccgttt gcgttcatga cgccggtgaa ggtggccggg tcgacggtgg gacaggccac cctgcacaac gcctcggaga tcaagcgcaa gggcgtgctg atcggcgaca ccgtggtgat ccgcaaggcc ggcgacgtga tccccgaggt gctgggaccc gtcgtcgaac tgcgcgatgg ctccgaacgc gaattcatca tgcccaccac ctgcccggag tgcggttcgc cgttggcgcc ggagaaggaa ggcgacgccg acatccgttg ccccaacgcc cgcggctgcc cggggcaact gcgggagcgg gttttccacg tcgccagccg caacggccta gacatcgagg tgctcggtta cgaggcgggt gtggcgctct tgcaggcgaa ggtgatcgcc gacgagggcg agctgttcgc gctgaccgag cgggacttgc tgcgcaccga cctgttccga accaaggcag gcgaactgtc ggccaacggc aaacggctgc tggtcaacct cgacaaggcc aaggcggcac cgctgtggcg ggtgctggtg gcgctgtcca tccgccatgt cgggccgacg gcggcccgcg ccctggccac cgagttcggc agccttgacg ccatcgccgc ggcgtccacc gaccagctgg ccgccgtcga gggggtgggg ccgaccattg ccgccgcggt caccgagtgg ttcgccgtcg actggcaccg cgagatcgtc gacaagtggc gggccgccgg ggtgcgaatg gtcgacgagc gtgacgagag tgtgccacgc acgctggccg ggctgaccat cgtggtcacc ggctcgctga ccggtttctc ccgcgacgac gccaaggagg cgatcgtggc ccgcggcggc aaggccgccg gctcggtgtc gaagaagacc aactatgtcg tcgccggaga ctcgccggga tccaaatacg acaaggcggt ggagttgggg gtgccgattc tggacgagga tgggttccgg agactgctgg ccgacggacc cgcgtcacga acgtaa
Details for MytuD.17946.a.B1.GE40372
| HARVESTED ON: | 7/15/2016 |
| SEQUENCED ON: | 7/18/2016 |
| EXPECTED MW: | 76kDa |
| OBSERVED MW: | 76kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | Low Expression |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMS SPDADQTAPE VLRQWQALAE EVREHQFRYY VRDAPIISDA EFDELLRRLE ALEEQHPELR TPDSPTQLVG GAGFATDFEP VDHLERMLSL DNAFTADELA AWAGRIHAEV GDAAHYLCEL KIDGVALSLV YREGRLTRAS TRGDGRTGED VTLNARTIAD VPERLTPGDD YPVPEVLEVR GEVFFRLDDF QALNASLVEE GKAPFANPRN SAAGSLRQKD PAVTARRRLR MICHGLGHVE GFRPATLHQA YLALRAWGLP VSEHTTLATD LAGVRERIDY WGEHRHEVDH EIDGVVVKVD EVALQRRLGS TSRAPRWAIA YKYPPEEAQT KLLDIRVNVG RTGRITPFAF MTPVKVAGST VGQATLHNAS EIKRKGVLIG DTVVIRKAGD VIPEVLGPVV ELRDGSEREF IMPTTCPECG SPLAPEKEGD ADIRCPNARG CPGQLRERVF HVASRNGLDI EVLGYEAGVA LLQAKVIADE GELFALTERD LLRTDLFRTK AGELSANGKR LLVNLDKAKA APLWRVLVAL SIRHVGPTAA RALATEFGSL DAIAAASTDQ LAAVEGVGPT IAAAVTEWFA VDWHREIVDK WRAAGVRMVD ERDESVPRTL AGLTIVVTGS LTGFSRDDAK EAIVARGGKA AGSVSKKTNY VVAGDSPGSK YDKAVELGVP ILDEDGFRRL LADGPASRT
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtgagct ccccagacgc cgatcagacc gctcccgagg tgttgcggca gtggcaggca ctggccgagg aggtgcgtga gcaccagttc cgttattacg tgcgggacgc gccgatcatc agcgacgcgg aattcgacga gctgctgcgc cgtctggaag ccctcgagga gcagcatccc gagctgcgca cgcccgattc gccgacccag ctggtcggcg gtgccggctt cgccacggat ttcgagcccg tcgaccatct cgaacgaatg ctcagcctcg acaacgcgtt caccgccgac gaactcgccg cctgggccgg ccgcatccat gccgaggtcg gagacgccgc acattacctg tgtgagctca agatcgacgg cgtcgcgctg tctttggtct accgcgaggg acggctgacc cgggcctcca cccgcggcga cgggcgcacc ggcgaggacg tcaccctgaa cgcccggacc atcgccgacg ttcccgaacg gctcaccccc ggcgacgact acccggtgcc cgaggtcctc gaggtccgcg gcgaggtctt cttccggctg gacgacttcc aggcgctcaa cgccagcctc gtcgaggagg gcaaggcgcc gttcgccaac ccccgcaaca gcgcggcggg atcgctgcgc cagaaagacc cggcggtcac cgcgcgccgc cggctgcgga tgatctgcca cgggctgggc cacgtggagg gctttcgccc ggccaccctg catcaggcat acctggcgtt gcgggcatgg ggactgccgg tttccgaaca caccaccctg gcaaccgacc tggccggtgt gcgcgagcgc atcgactact ggggcgagca ccgccacgag gtggaccacg aaatcgacgg cgtggtggtc aaagtcgacg aggtggcgtt gcagcgcagg ctgggttcca cgtcgcgggc gccgcgctgg gccatcgcct acaagtaccc gcccgaggaa gcgcagacca agctgctcga catccgggtg aacgtcggcc gcaccgggcg gatcacgccg tttgcgttca tgacgccggt gaaggtggcc gggtcgacgg tgggacaggc caccctgcac aacgcctcgg agatcaagcg caagggcgtg ctgatcggcg acaccgtggt gatccgcaag gccggcgacg tgatccccga ggtgctggga cccgtcgtcg aactgcgcga tggctccgaa cgcgaattca tcatgcccac cacctgcccg gagtgcggtt cgccgttggc gccggagaag gaaggcgacg ccgacatccg ttgccccaac gcccgcggct gcccggggca actgcgggag cgggttttcc acgtcgccag ccgcaacggc ctagacatcg aggtgctcgg ttacgaggcg ggtgtggcgc tcttgcaggc gaaggtgatc gccgacgagg gcgagctgtt cgcgctgacc gagcgggact tgctgcgcac cgacctgttc cgaaccaagg caggcgaact gtcggccaac ggcaaacggc tgctggtcaa cctcgacaag gccaaggcgg caccgctgtg gcgggtgctg gtggcgctgt ccatccgcca tgtcgggccg acggcggccc gcgccctggc caccgagttc ggcagccttg acgccatcgc cgcggcgtcc accgaccagc tggccgccgt cgagggggtg gggccgacca ttgccgccgc ggtcaccgag tggttcgccg tcgactggca ccgcgagatc gtcgacaagt ggcgggccgc cggggtgcga atggtcgacg agcgtgacga gagtgtgcca cgcacgctgg ccgggctgac catcgtggtc accggctcgc tgaccggttt ctcccgcgac gacgccaagg aggcgatcgt ggcccgcggc ggcaaggccg ccggctcggt gtcgaagaag accaactatg tcgtcgccgg agactcgccg ggatccaaat acgacaaggc ggtggagttg ggggtgccga ttctggacga ggatgggttc cggagactgc tggccgacgg acccgcgtca cgaacgtgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t
Details for MytuD.17946.a.B2.GE40367
| HARVESTED ON: | 7/15/2016 |
| SEQUENCED ON: | 7/18/2016 |
| EXPECTED MW: | 38kDa |
| OBSERVED MW: | 38kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMS SPDADQTAPE VLRQWQALAE EVREHQFRYY VRDAPIISDA EFDELLRRLE ALEEQHPELR TPDSPTQLVG GAGFATDFEP VDHLERMLSL DNAFTADELA AWAGRIHAEV GDAAHYLCEL KIDGVALSLV YREGRLTRAS TRGDGRTGED VTLNARTIAD VPERLTPGDD YPVPEVLEVR GEVFFRLDDF QALNASLVEE GKAPFANPRN SAAGSLRQKD PAVTARRRLR MICHGLGHVE GFRPATLHQA YLALRAWGLP VSEHTTLATD LAGVRERIDY WGEHRHEVDH EIDGVVVKVD EVALQRRLGS TSRAPRWAIA YKYPPE
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtgagct ccccagacgc cgatcagacc gctcccgagg tgttgcggca gtggcaggca ctggccgagg aggtgcgtga gcaccagttc cgttattacg tgcgggacgc gccgatcatc agcgacgcgg aattcgacga gctgctgcgc cgtctggaag ccctcgagga gcagcatccc gagctgcgca cgcccgattc gccgacccag ctggtcggcg gtgccggctt cgccacggat ttcgagcccg tcgaccatct cgaacgaatg ctcagcctcg acaacgcgtt caccgccgac gaactcgccg cctgggccgg ccgcatccat gccgaggtcg gagacgccgc acattacctg tgtgagctca agatcgacgg cgtcgcgctg tctttggtct accgcgaggg acggctgacc cgggcctcca cccgcggcga cgggcgcacc ggcgaggacg tcaccctgaa cgcccggacc atcgccgacg ttcccgaacg gctcaccccc ggcgacgact acccggtgcc cgaggtcctc gaggtccgcg gcgaggtctt cttccggctg gacgacttcc aggcgctcaa cgccagcctc gtcgaggagg gcaaggcgcc gttcgccaac ccccgcaaca gcgcggcggg atcgctgcgc cagaaagacc cggcggtcac cgcgcgccgc cggctgcgga tgatctgcca cgggctgggc cacgtggagg gctttcgccc ggccaccctg catcaggcat acctggcgtt gcgggcatgg ggactgccgg tttccgaaca caccaccctg gcaaccgacc tggccggtgt gcgcgagcgc atcgactact ggggcgagca ccgccacgag gtggaccacg aaatcgacgg cgtggtggtc aaagtcgacg aggtggcgtt gcagcgcagg ctgggttcca cgtcgcgggc gccgcgctgg gccatcgcct acaagtaccc gcccgagtga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at
Details for MytuD.17946.a.B3.GE40368
| HARVESTED ON: | 7/15/2016 |
| SEQUENCED ON: | 7/18/2016 |
| EXPECTED MW: | 67kDa |
| OBSERVED MW: | 67kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHSS PDADQTAPEV LRQWQALAEE VREHQFRYYV RDAPIISDAE FDELLRRLEA LEEQHPELRT PDSPTQLVGG AGFATDFEPV DHLERMLSLD NAFTADELAA WAGRIHAEVG DAAHYLCELK IDGVALSLVY REGRLTRAST RGDGRTGEDV TLNARTIADV PERLTPGDDY PVPEVLEVRG EVFFRLDDFQ ALNASLVEEG KAPFANPRNS AAGSLRQKDP AVTARRRLRM ICHGLGHVEG FRPATLHQAY LALRAWGLPV SEHTTLATDL AGVRERIDYW GEHRHEVDHE IDGVVVKVDE VALQRRLGST SRAPRWAIAY KYPPEEAQTK LLDIRVNVGR TGRITPFAFM TPVKVAGSTV GQATLHNASE IKRKGVLIGD TVVIRKAGDV IPEVLGPVVE LRDGSEREFI MPTTCPECGS PLAPEKEGDA DIRCPNARGC PGQLRERVFH VASRNGLDIE VLGYEAGVAL LQAKVIADEG ELFALTERDL LRTDLFRTKA GELSANGKRL LVNLDKAKAA PLWRVLVALS IRHVGPTAAR ALATEFGSLD AIAAASTDQL AAVEGVGPTI AAAVTEWFAV DWHREIVDKW RAAGVRMVDE RD
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catagctccc cagacgccga tcagaccgct cccgaggtgt tgcggcagtg gcaggcactg gccgaggagg tgcgtgagca ccagttccgt tattacgtgc gggacgcgcc gatcatcagc gacgcggaat tcgacgagct gctgcgccgt ctggaagccc tcgaggagca gcatcccgag ctgcgcacgc ccgattcgcc gacccagctg gtcggcggtg ccggcttcgc cacggatttc gagcccgtcg accatctcga acgaatgctc agcctcgaca acgcgttcac cgccgacgaa ctcgccgcct gggccggccg catccatgcc gaggtcggag acgccgcaca ttacctgtgt gagctcaaga tcgacggcgt cgcgctgtct ttggtctacc gcgagggacg gctgacccgg gcctccaccc gcggcgacgg gcgcaccggc gaggacgtca ccctgaacgc ccggaccatc gccgacgttc ccgaacggct cacccccggc gacgactacc cggtgcccga ggtcctcgag gtccgcggcg aggtcttctt ccggctggac gacttccagg cgctcaacgc cagcctcgtc gaggagggca aggcgccgtt cgccaacccc cgcaacagcg cggcgggatc gctgcgccag aaagacccgg cggtcaccgc gcgccgccgg ctgcggatga tctgccacgg gctgggccac gtggagggct ttcgcccggc caccctgcat caggcatacc tggcgttgcg ggcatgggga ctgccggttt ccgaacacac caccctggca accgacctgg ccggtgtgcg cgagcgcatc gactactggg gcgagcaccg ccacgaggtg gaccacgaaa tcgacggcgt ggtggtcaaa gtcgacgagg tggcgttgca gcgcaggctg ggttccacgt cgcgggcgcc gcgctgggcc atcgcctaca agtacccgcc cgaggaagcg cagaccaagc tgctcgacat ccgggtgaac gtcggccgca ccgggcggat cacgccgttt gcgttcatga cgccggtgaa ggtggccggg tcgacggtgg gacaggccac cctgcacaac gcctcggaga tcaagcgcaa gggcgtgctg atcggcgaca ccgtggtgat ccgcaaggcc ggcgacgtga tccccgaggt gctgggaccc gtcgtcgaac tgcgcgatgg ctccgaacgc gaattcatca tgcccaccac ctgcccggag tgcggttcgc cgttggcgcc ggagaaggaa ggcgacgccg acatccgttg ccccaacgcc cgcggctgcc cggggcaact gcgggagcgg gttttccacg tcgccagccg caacggccta gacatcgagg tgctcggtta cgaggcgggt gtggcgctct tgcaggcgaa ggtgatcgcc gacgagggcg agctgttcgc gctgaccgag cgggacttgc tgcgcaccga cctgttccga accaaggcag gcgaactgtc ggccaacggc aaacggctgc tggtcaacct cgacaaggcc aaggcggcac cgctgtggcg ggtgctggtg gcgctgtcca tccgccatgt cgggccgacg gcggcccgcg ccctggccac cgagttcggc agccttgacg ccatcgccgc ggcgtccacc gaccagctgg ccgccgtcga gggggtgggg ccgaccattg ccgccgcggt caccgagtgg ttcgccgtcg actggcaccg cgagatcgtc gacaagtggc gggccgccgg ggtgcgaatg gtcgacgagc gtgactgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat
Details for MytuD.17946.a.B4.GE40369
| HARVESTED ON: | 7/15/2016 |
| SEQUENCED ON: | 7/18/2016 |
| EXPECTED MW: | 59kDa |
| OBSERVED MW: | 59kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Low Expression |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHTD FEPVDHLERM LSLDNAFTAD ELAAWAGRIH AEVGDAAHYL CELKIDGVAL SLVYREGRLT RASTRGDGRT GEDVTLNART IADVPERLTP GDDYPVPEVL EVRGEVFFRL DDFQALNASL VEEGKAPFAN PRNSAAGSLR QKDPAVTARR RLRMICHGLG HVEGFRPATL HQAYLALRAW GLPVSEHTTL ATDLAGVRER IDYWGEHRHE VDHEIDGVVV KVDEVALQRR LGSTSRAPRW AIAYKYPPEE AQTKLLDIRV NVGRTGRITP FAFMTPVKVA GSTVGQATLH NASEIKRKGV LIGDTVVIRK AGDVIPEVLG PVVELRDGSE REFIMPTTCP ECGSPLAPEK EGDADIRCPN ARGCPGQLRE RVFHVASRNG LDIEVLGYEA GVALLQAKVI ADEGELFALT ERDLLRTDLF RTKAGELSAN GKRLLVNLDK AKAAPLWRVL VALSIRHVGP TAARALATEF GSLDAIAAAS TDQLAAVEGV GPTIAAAVTE WFAVDWHREI VDKWRAAGVR MVDERD
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catacggatt tcgagcccgt cgaccatctc gaacgaatgc tcagcctcga caacgcgttc accgccgacg aactcgccgc ctgggccggc cgcatccatg ccgaggtcgg agacgccgca cattacctgt gtgagctcaa gatcgacggc gtcgcgctgt ctttggtcta ccgcgaggga cggctgaccc gggcctccac ccgcggcgac gggcgcaccg gcgaggacgt caccctgaac gcccggacca tcgccgacgt tcccgaacgg ctcacccccg gcgacgacta cccggtgccc gaggtcctcg aggtccgcgg cgaggtcttc ttccggctgg acgacttcca ggcgctcaac gccagcctcg tcgaggaggg caaggcgccg ttcgccaacc cccgcaacag cgcggcggga tcgctgcgcc agaaagaccc ggcggtcacc gcgcgccgcc ggctgcggat gatctgccac gggctgggcc acgtggaggg ctttcgcccg gccaccctgc atcaggcata cctggcgttg cgggcatggg gactgccggt ttccgaacac accaccctgg caaccgacct ggccggtgtg cgcgagcgca tcgactactg gggcgagcac cgccacgagg tggaccacga aatcgacggc gtggtggtca aagtcgacga ggtggcgttg cagcgcaggc tgggttccac gtcgcgggcg ccgcgctggg ccatcgccta caagtacccg cccgaggaag cgcagaccaa gctgctcgac atccgggtga acgtcggccg caccgggcgg atcacgccgt ttgcgttcat gacgccggtg aaggtggccg ggtcgacggt gggacaggcc accctgcaca acgcctcgga gatcaagcgc aagggcgtgc tgatcggcga caccgtggtg atccgcaagg ccggcgacgt gatccccgag gtgctgggac ccgtcgtcga actgcgcgat ggctccgaac gcgaattcat catgcccacc acctgcccgg agtgcggttc gccgttggcg ccggagaagg aaggcgacgc cgacatccgt tgccccaacg cccgcggctg cccggggcaa ctgcgggagc gggttttcca cgtcgccagc cgcaacggcc tagacatcga ggtgctcggt tacgaggcgg gtgtggcgct cttgcaggcg aaggtgatcg ccgacgaggg cgagctgttc gcgctgaccg agcgggactt gctgcgcacc gacctgttcc gaaccaaggc aggcgaactg tcggccaacg gcaaacggct gctggtcaac ctcgacaagg ccaaggcggc accgctgtgg cgggtgctgg tggcgctgtc catccgccat gtcgggccga cggcggcccg cgccctggcc accgagttcg gcagccttga cgccatcgcc gcggcgtcca ccgaccagct ggccgccgtc gagggggtgg ggccgaccat tgccgccgcg gtcaccgagt ggttcgccgt cgactggcac cgcgagatcg tcgacaagtg gcgggccgcc ggggtgcgaa tggtcgacga gcgtgactga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at
Details for MytuD.17946.a.B5.GE40370
| HARVESTED ON: | 7/15/2016 |
| SEQUENCED ON: | 7/18/2016 |
| EXPECTED MW: | 29kDa |
| OBSERVED MW: | 30kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | Low Expression |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHTD FEPVDHLERM LSLDNAFTAD ELAAWAGRIH AEVGDAAHYL CELKIDGVAL SLVYREGRLT RASTRGDGRT GEDVTLNART IADVPERLTP GDDYPVPEVL EVRGEVFFRL DDFQALNASL VEEGKAPFAN PRNSAAGSLR QKDPAVTARR RLRMICHGLG HVEGFRPATL HQAYLALRAW GLPVSEHTTL ATDLAGVRER IDYWGEHRHE VDHEIDGVVV KVDEVALQRR LGSTSRAPRW AIAYKYPPE
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catacggatt tcgagcccgt cgaccatctc gaacgaatgc tcagcctcga caacgcgttc accgccgacg aactcgccgc ctgggccggc cgcatccatg ccgaggtcgg agacgccgca cattacctgt gtgagctcaa gatcgacggc gtcgcgctgt ctttggtcta ccgcgaggga cggctgaccc gggcctccac ccgcggcgac gggcgcaccg gcgaggacgt caccctgaac gcccggacca tcgccgacgt tcccgaacgg ctcacccccg gcgacgacta cccggtgccc gaggtcctcg aggtccgcgg cgaggtcttc ttccggctgg acgacttcca ggcgctcaac gccagcctcg tcgaggaggg caaggcgccg ttcgccaacc cccgcaacag cgcggcggga tcgctgcgcc agaaagaccc ggcggtcacc gcgcgccgcc ggctgcggat gatctgccac gggctgggcc acgtggaggg ctttcgcccg gccaccctgc atcaggcata cctggcgttg cgggcatggg gactgccggt ttccgaacac accaccctgg caaccgacct ggccggtgtg cgcgagcgca tcgactactg gggcgagcac cgccacgagg tggaccacga aatcgacggc gtggtggtca aagtcgacga ggtggcgttg cagcgcaggc tgggttccac gtcgcgggcg ccgcgctggg ccatcgccta caagtacccg cccgagtgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t
Details for MytuD.17946.a.B6.GE40371
| HARVESTED ON: | 7/15/2016 |
| SEQUENCED ON: | 7/18/2016 |
| EXPECTED MW: | 47kDa |
| OBSERVED MW: | 47kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | Low Expression |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMS SPDADQTAPE VLRQWQALAE EVREHQFRYY VRDAPIISDA EFDELLRRLE ALEEQHPELR TPDSPTQLVG GAGFATDFEP VDHLERMLSL DNAFTADELA AWAGRIHAEV GDAAHYLCEL KIDGVALSLV YREGRLTRAS TRGDGRTGED VTLNARTIAD VPERLTPGDD YPVPEVLEVR GEVFFRLDDF QALNASLVEE GKAPFANPRN SAAGSLRQKD PAVTARRRLR MICHGLGHVE GFRPATLHQA YLALRAWGLP VSEHTTLATD LAGVRERIDY WGEHRHEVDH EIDGVVVKVD EVALQRRLGS TSRAPRWAIA YKYPPEEAQT KLLDIRVNVG RTGRITPFAF MTPVKVAGST VGQATLHNAS EIKRKGVLIG DTVVIRKAGD VIPEVLGPVV ELRDGSEREF IMPTT
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtgagct ccccagacgc cgatcagacc gctcccgagg tgttgcggca gtggcaggca ctggccgagg aggtgcgtga gcaccagttc cgttattacg tgcgggacgc gccgatcatc agcgacgcgg aattcgacga gctgctgcgc cgtctggaag ccctcgagga gcagcatccc gagctgcgca cgcccgattc gccgacccag ctggtcggcg gtgccggctt cgccacggat ttcgagcccg tcgaccatct cgaacgaatg ctcagcctcg acaacgcgtt caccgccgac gaactcgccg cctgggccgg ccgcatccat gccgaggtcg gagacgccgc acattacctg tgtgagctca agatcgacgg cgtcgcgctg tctttggtct accgcgaggg acggctgacc cgggcctcca cccgcggcga cgggcgcacc ggcgaggacg tcaccctgaa cgcccggacc atcgccgacg ttcccgaacg gctcaccccc ggcgacgact acccggtgcc cgaggtcctc gaggtccgcg gcgaggtctt cttccggctg gacgacttcc aggcgctcaa cgccagcctc gtcgaggagg gcaaggcgcc gttcgccaac ccccgcaaca gcgcggcggg atcgctgcgc cagaaagacc cggcggtcac cgcgcgccgc cggctgcgga tgatctgcca cgggctgggc cacgtggagg gctttcgccc ggccaccctg catcaggcat acctggcgtt gcgggcatgg ggactgccgg tttccgaaca caccaccctg gcaaccgacc tggccggtgt gcgcgagcgc atcgactact ggggcgagca ccgccacgag gtggaccacg aaatcgacgg cgtggtggtc aaagtcgacg aggtggcgtt gcagcgcagg ctgggttcca cgtcgcgggc gccgcgctgg gccatcgcct acaagtaccc gcccgaggaa gcgcagacca agctgctcga catccgggtg aacgtcggcc gcaccgggcg gatcacgccg tttgcgttca tgacgccggt gaaggtggcc gggtcgacgg tgggacaggc caccctgcac aacgcctcgg agatcaagcg caagggcgtg ctgatcggcg acaccgtggt gatccgcaag gccggcgacg tgatccccga ggtgctggga cccgtcgtcg aactgcgcga tggctccgaa cgcgaattca tcatgcccac cacctgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat
Details for MytuD.17946.a.B3.PS38124
| PURIFICATION DATe: | 11/22/2016 |
| CONCENTRATION: | 40.7mg/ml |
| OBSERVED MW: | 67kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | SEC Buffer |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| VIAL COUNT (approx.): | 2 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MAHHHHHHSS PDADQTAPEV LRQWQALAEE VREHQFRYYV RDAPIISDAE FDELLRRLEA LEEQHPELRT PDSPTQLVGG AGFATDFEPV DHLERMLSLD NAFTADELAA WAGRIHAEVG DAAHYLCELK IDGVALSLVY REGRLTRAST RGDGRTGEDV TLNARTIADV PERLTPGDDY PVPEVLEVRG EVFFRLDDFQ ALNASLVEEG KAPFANPRNS AAGSLRQKDP AVTARRRLRM ICHGLGHVEG FRPATLHQAY LALRAWGLPV SEHTTLATDL AGVRERIDYW GEHRHEVDHE IDGVVVKVDE VALQRRLGST SRAPRWAIAY KYPPEEAQTK LLDIRVNVGR TGRITPFAFM TPVKVAGSTV GQATLHNASE IKRKGVLIGD TVVIRKAGDV IPEVLGPVVE LRDGSEREFI MPTTCPECGS PLAPEKEGDA DIRCPNARGC PGQLRERVFH VASRNGLDIE VLGYEAGVAL LQAKVIADEG ELFALTERDL LRTDLFRTKA GELSANGKRL LVNLDKAKAA PLWRVLVALS IRHVGPTAAR ALATEFGSLD AIAAASTDQL AAVEGVGPTI AAAVTEWFAV DWHREIVDKW RAAGVRMVDE RD
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATAGCTCCC CAGACGCCGA TCAGACCGCT CCCGAGGTGT TGCGGCAGTG GCAGGCACTG GCCGAGGAGG TGCGTGAGCA CCAGTTCCGT TATTACGTGC GGGACGCGCC GATCATCAGC GACGCGGAAT TCGACGAGCT GCTGCGCCGT CTGGAAGCCC TCGAGGAGCA GCATCCCGAG CTGCGCACGC CCGATTCGCC GACCCAGCTG GTCGGCGGTG CCGGCTTCGC CACGGATTTC GAGCCCGTCG ACCATCTCGA ACGAATGCTC AGCCTCGACA ACGCGTTCAC CGCCGACGAA CTCGCCGCCT GGGCCGGCCG CATCCATGCC GAGGTCGGAG ACGCCGCACA TTACCTGTGT GAGCTCAAGA TCGACGGCGT CGCGCTGTCT TTGGTCTACC GCGAGGGACG GCTGACCCGG GCCTCCACCC GCGGCGACGG GCGCACCGGC GAGGACGTCA CCCTGAACGC CCGGACCATC GCCGACGTTC CCGAACGGCT CACCCCCGGC GACGACTACC CGGTGCCCGA GGTCCTCGAG GTCCGCGGCG AGGTCTTCTT CCGGCTGGAC GACTTCCAGG CGCTCAACGC CAGCCTCGTC GAGGAGGGCA AGGCGCCGTT CGCCAACCCC CGCAACAGCG CGGCGGGATC GCTGCGCCAG AAAGACCCGG CGGTCACCGC GCGCCGCCGG CTGCGGATGA TCTGCCACGG GCTGGGCCAC GTGGAGGGCT TTCGCCCGGC CACCCTGCAT CAGGCATACC TGGCGTTGCG GGCATGGGGA CTGCCGGTTT CCGAACACAC CACCCTGGCA ACCGACCTGG CCGGTGTGCG CGAGCGCATC GACTACTGGG GCGAGCACCG CCACGAGGTG GACCACGAAA TCGACGGCGT GGTGGTCAAA GTCGACGAGG TGGCGTTGCA GCGCAGGCTG GGTTCCACGT CGCGGGCGCC GCGCTGGGCC ATCGCCTACA AGTACCCGCC CGAGGAAGCG CAGACCAAGC TGCTCGACAT CCGGGTGAAC GTCGGCCGCA CCGGGCGGAT CACGCCGTTT GCGTTCATGA CGCCGGTGAA GGTGGCCGGG TCGACGGTGG GACAGGCCAC CCTGCACAAC GCCTCGGAGA TCAAGCGCAA GGGCGTGCTG ATCGGCGACA CCGTGGTGAT CCGCAAGGCC GGCGACGTGA TCCCCGAGGT GCTGGGACCC GTCGTCGAAC TGCGCGATGG CTCCGAACGC GAATTCATCA TGCCCACCAC CTGCCCGGAG TGCGGTTCGC CGTTGGCGCC GGAGAAGGAA GGCGACGCCG ACATCCGTTG CCCCAACGCC CGCGGCTGCC CGGGGCAACT GCGGGAGCGG GTTTTCCACG TCGCCAGCCG CAACGGCCTA GACATCGAGG TGCTCGGTTA CGAGGCGGGT GTGGCGCTCT TGCAGGCGAA GGTGATCGCC GACGAGGGCG AGCTGTTCGC GCTGACCGAG CGGGACTTGC TGCGCACCGA CCTGTTCCGA ACCAAGGCAG GCGAACTGTC GGCCAACGGC AAACGGCTGC TGGTCAACCT CGACAAGGCC AAGGCGGCAC CGCTGTGGCG GGTGCTGGTG GCGCTGTCCA TCCGCCATGT CGGGCCGACG GCGGCCCGCG CCCTGGCCAC CGAGTTCGGC AGCCTTGACG CCATCGCCGC GGCGTCCACC GACCAGCTGG CCGCCGTCGA GGGGGTGGGG CCGACCATTG CCGCCGCGGT CACCGAGTGG TTCGCCGTCG ACTGGCACCG CGAGATCGTC GACAAGTGGC GGGCCGCCGG GGTGCGAATG GTCGACGAGC GTGACTGAGT AAGATAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA ATTCTTGAAG ACGAAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC TTAGACGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTGTTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC CTGCAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCTGAT GCGGTATTTT CTCCTTACGC ATCTGTGCGG TATTTCACAC CGCATATATG GTGCACTCTC AGTACAATCT GCTCTGATGC CGCATAGTTA AGCCAGTATA CACTCCGCTA TCGCTACGTG ACTGGGTCAT GGCTGCGCCC CGACACCCGC CAACACCCGC TGACGCGCCC TGACGGGCTT GTCTGCTCCC GGCATCCGCT TACAGACAAG CTGTGACCGT CTCCGGGAGC TGCATGTGTC AGAGGTTTTC ACCGTCATCA CCGAAACGCG CGAGGCAGCT GCGGTAAAGC TCATCAGCGT GGTCGTGAAG CGATTCACAG ATGTCTGCCT GTTCATCCGC GTCCAGCTCG TTGAGTTTCT CCAGAAGCGT TAATGTCTGG CTTCTGATAA AGCGGGCCAT GTTAAGGGCG GTTTTTTCCT GTTTGGTCAC TGATGCCTCC GTGTAAGGGG GATTTCTGTT CATGGGGGTA ATGATACCGA TGAAACGAGA GAGGATGCTC ACGATACGGG TTACTGATGA TGAACATGCC CGGTTACTGG AACGTTGTGA GGGTAAACAA CTGGCGGTAT GGATGCGGCG GGACCAGAGA AAAATCACTC AGGGTCAATG CCAGCGCTTC GTTAATACAG ATGTAGGTGT TCCACAGGGT AGCCAGCAGC ATCCTGCGAT GCAGATCCGG AACATAATGG TGCAGGGCGC TGACTTCCGC GTTTCCAGAC TTTACGAAAC ACGGAAACCG AAGACCATTC ATGTTGTTGC TCAGGTCGCA GACGTTTTGC AGCAGCAGTC GCTTCACGTT CGCTCGCGTA TCGGTGATTC ATTCTGCTAA CCAGTAAGGC AACCCCGCCA GCCTAGCCGG GTCCTCAACG ACAGGAGCAC GATCATGCGC ACCCGTGGCC AGGACCCAAC GCTGCCCGAG ATGCGCCGCG TGCGGCTGCT GGAGATGGCG GACGCGATGG ATATGTTCTG CCAAGGGTTG GTTTGCGCAT TCACAGTTCT CCGCAAGAAT TGATTGGCTC CAATTCTTGG AGTGGTGAAT CCGTTAGCGA GGTGCCGCCG GCTTCCATTC AGGTCGAGGT GGCCCGGCTC CATGCACCGC GACGCAACGC GGGGAGGCAG ACAAGGTATA GGGCGGCGCC TACAATCCAT GCCAACCCGT TCCATGTGCT CGCCGAGGCG GCATAAATCG CCGTGACGAT CAGCGGTCCA GTGATCGAAG TTAGGCTGGT AAGAGCCGCG AGCGATCCTT GAAGCTGTCC CTGATGGTCG TCATCTACCT GCCTGGACAG CATGGCCTGC AACGCGGGCA TCCCGATGCC GCCGGAAGCG AGAAGAATCA TAATGGGGAA GGCCATCCAG CCTCGCGTCG CGAACGCCAG CAAGACGTAG CCCAGCGCGT CGGCCGCCAT GCCGGCGATA ATGGCCTGCT TCTCGCCGAA ACGTTTGGTG GCGGGACCAG TGACGAAGGC TTGAGCGAGG GCGTGCAAGA TTCCGAATAC CGCAAGCGAC AGGCCGATCA TCGTCGCGCT CCAGCGAAAG CGGTCCTCGC CGAAAATGAC CCAGAGCGCT GCCGGCACCT GTCCTACGAG TTGCATGATA AAGAAGACAG TCATAAGTGC GGCGACGATA GTCATGCCCC GCGCCCACCG GAAGGAGCTG ACTGGGTTGA AGGCTCTCAA GGGCATCGGT CGACGCTCTC CCTTATGCGA CTCCTGCATT AGGAAGCAGC CCAGTAGTAG GTTGAGGCCG TTGAGCACCG CCGCCGCAAG GAATGGTGCA TGCAAGGAGA TGGCGCCCAA CAGTCCCCCG GCCACGGGGC CTGCCACCAT ACCCACGCCG AAACAAGCGC TCATGAGCCC GAAGTGGCGA GCCCGATCTT CCCCATCGGT GATGTCGGCG ATATAGGCGC CAGCAACCGC ACCTGTGGCG CCGGTGATGC CGGCCACGAT GCGTCCGGCG TAGAGGATCG AGATCTCGAT CCCGCGAAAT
Details for MytuD.17946.a.B2.PS38127
| PURIFICATION DATe: | 11/17/2016 |
| CONCENTRATION: | 21.72mg/ml |
| OBSERVED MW: | 38kDa |
| EXPRESSION LEVEL: | Low Expression |
| PROTEIN PURIFICATION BUFFER: | SEC |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 3 |
| VIAL VOLUME: | 110µl |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MAHHHHHHMS SPDADQTAPE VLRQWQALAE EVREHQFRYY VRDAPIISDA EFDELLRRLE ALEEQHPELR TPDSPTQLVG GAGFATDFEP VDHLERMLSL DNAFTADELA AWAGRIHAEV GDAAHYLCEL KIDGVALSLV YREGRLTRAS TRGDGRTGED VTLNARTIAD VPERLTPGDD YPVPEVLEVR GEVFFRLDDF QALNASLVEE GKAPFANPRN SAAGSLRQKD PAVTARRRLR MICHGLGHVE GFRPATLHQA YLALRAWGLP VSEHTTLATD LAGVRERIDY WGEHRHEVDH EIDGVVVKVD EVALQRRLGS TSRAPRWAIA YKYPPE
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATGTGAGCT CCCCAGACGC CGATCAGACC GCTCCCGAGG TGTTGCGGCA GTGGCAGGCA CTGGCCGAGG AGGTGCGTGA GCACCAGTTC CGTTATTACG TGCGGGACGC GCCGATCATC AGCGACGCGG AATTCGACGA GCTGCTGCGC CGTCTGGAAG CCCTCGAGGA GCAGCATCCC GAGCTGCGCA CGCCCGATTC GCCGACCCAG CTGGTCGGCG GTGCCGGCTT CGCCACGGAT TTCGAGCCCG TCGACCATCT CGAACGAATG CTCAGCCTCG ACAACGCGTT CACCGCCGAC GAACTCGCCG CCTGGGCCGG CCGCATCCAT GCCGAGGTCG GAGACGCCGC ACATTACCTG TGTGAGCTCA AGATCGACGG CGTCGCGCTG TCTTTGGTCT ACCGCGAGGG ACGGCTGACC CGGGCCTCCA CCCGCGGCGA CGGGCGCACC GGCGAGGACG TCACCCTGAA CGCCCGGACC ATCGCCGACG TTCCCGAACG GCTCACCCCC GGCGACGACT ACCCGGTGCC CGAGGTCCTC GAGGTCCGCG GCGAGGTCTT CTTCCGGCTG GACGACTTCC AGGCGCTCAA CGCCAGCCTC GTCGAGGAGG GCAAGGCGCC GTTCGCCAAC CCCCGCAACA GCGCGGCGGG ATCGCTGCGC CAGAAAGACC CGGCGGTCAC CGCGCGCCGC CGGCTGCGGA TGATCTGCCA CGGGCTGGGC CACGTGGAGG GCTTTCGCCC GGCCACCCTG CATCAGGCAT ACCTGGCGTT GCGGGCATGG GGACTGCCGG TTTCCGAACA CACCACCCTG GCAACCGACC TGGCCGGTGT GCGCGAGCGC ATCGACTACT GGGGCGAGCA CCGCCACGAG GTGGACCACG AAATCGACGG CGTGGTGGTC AAAGTCGACG AGGTGGCGTT GCAGCGCAGG CTGGGTTCCA CGTCGCGGGC GCCGCGCTGG GCCATCGCCT ACAAGTACCC GCCCGAGTGA GTAAGATAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAATTCTTGA AGACGAAAGG GCCTCGTGAT ACGCCTATTT TTATAGGTTA ATGTCATGAT AATAATGGTT TCTTAGACGT CAGGTGGCAC TTTTCGGGGA AATGTGCGCG GAACCCCTAT TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT AACCCTGATA AATGCTTCAA TAATATTGAA AAAGGAAGAG TATGAGTATT CAACATTTCC GTGTCGCCCT TATTCCCTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT CACCCAGAAA CGCTGGTGAA AGTAAAAGAT GCTGAAGATC AGTTGGGTGC ACGAGTGGGT TACATCGAAC TGGATCTCAA CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC CGAAGAACGT TTTCCAATGA TGAGCACTTT TAAAGTTCTG CTATGTGGCG CGGTATTATC CCGTGTTGAC GCCGGGCAAG AGCAACTCGG TCGCCGCATA CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA CAGAAAAGCA TCTTACGGAT GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA TGAGTGATAA CACTGCGGCC AACTTACTTC TGACAACGAT CGGAGGACCG AAGGAGCTAA CCGCTTTTTT GCACAACATG GGGGATCATG TAACTCGCCT TGATCGTTGG GAACCGGAGC TGAATGAAGC CATACCAAAC GACGAGCGTG ACACCACGAT GCCTGCAGCA ATGGCAACAA CGTTGCGCAA ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG ACTGGATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGGTC TCGCGGTATC ATTGCAGCAC TGGGGCCAGA TGGTAAGCCC TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA CTATGGATGA ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT AACTGTCAGA CCAAGTTTAC TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTC CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC GAACGACCGA GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA AGAGCGCCTG ATGCGGTATT TTCTCCTTAC GCATCTGTGC GGTATTTCAC ACCGCATATA TGGTGCACTC TCAGTACAAT CTGCTCTGAT GCCGCATAGT TAAGCCAGTA TACACTCCGC TATCGCTACG TGACTGGGTC ATGGCTGCGC CCCGACACCC GCCAACACCC GCTGACGCGC CCTGACGGGC TTGTCTGCTC CCGGCATCCG CTTACAGACA AGCTGTGACC GTCTCCGGGA GCTGCATGTG TCAGAGGTTT TCACCGTCAT CACCGAAACG CGCGAGGCAG CTGCGGTAAA GCTCATCAGC GTGGTCGTGA AGCGATTCAC AGATGTCTGC CTGTTCATCC GCGTCCAGCT CGTTGAGTTT CTCCAGAAGC GTTAATGTCT GGCTTCTGAT AAAGCGGGCC ATGTTAAGGG CGGTTTTTTC CTGTTTGGTC ACTGATGCCT CCGTGTAAGG GGGATTTCTG TTCATGGGGG TAATGATACC GATGAAACGA GAGAGGATGC TCACGATACG GGTTACTGAT GATGAACATG CCCGGTTACT GGAACGTTGT GAGGGTAAAC AACTGGCGGT ATGGATGCGG CGGGACCAGA GAAAAATCAC TCAGGGTCAA TGCCAGCGCT TCGTTAATAC AGATGTAGGT GTTCCACAGG GTAGCCAGCA GCATCCTGCG ATGCAGATCC GGAACATAAT GGTGCAGGGC GCTGACTTCC GCGTTTCCAG ACTTTACGAA ACACGGAAAC CGAAGACCAT TCATGTTGTT GCTCAGGTCG CAGACGTTTT GCAGCAGCAG TCGCTTCACG TTCGCTCGCG TATCGGTGAT TCATTCTGCT AACCAGTAAG GCAACCCCGC CAGCCTAGCC GGGTCCTCAA CGACAGGAGC ACGATCATGC GCACCCGTGG CCAGGACCCA ACGCTGCCCG AGATGCGCCG CGTGCGGCTG CTGGAGATGG CGGACGCGAT GGATATGTTC TGCCAAGGGT TGGTTTGCGC ATTCACAGTT CTCCGCAAGA ATTGATTGGC TCCAATTCTT GGAGTGGTGA ATCCGTTAGC GAGGTGCCGC CGGCTTCCAT TCAGGTCGAG GTGGCCCGGC TCCATGCACC GCGACGCAAC GCGGGGAGGC AGACAAGGTA TAGGGCGGCG CCTACAATCC ATGCCAACCC GTTCCATGTG CTCGCCGAGG CGGCATAAAT CGCCGTGACG ATCAGCGGTC CAGTGATCGA AGTTAGGCTG GTAAGAGCCG CGAGCGATCC TTGAAGCTGT CCCTGATGGT CGTCATCTAC CTGCCTGGAC AGCATGGCCT GCAACGCGGG CATCCCGATG CCGCCGGAAG CGAGAAGAAT CATAATGGGG AAGGCCATCC AGCCTCGCGT CGCGAACGCC AGCAAGACGT AGCCCAGCGC GTCGGCCGCC ATGCCGGCGA TAATGGCCTG CTTCTCGCCG AAACGTTTGG TGGCGGGACC AGTGACGAAG GCTTGAGCGA GGGCGTGCAA GATTCCGAAT ACCGCAAGCG ACAGGCCGAT CATCGTCGCG CTCCAGCGAA AGCGGTCCTC GCCGAAAATG ACCCAGAGCG CTGCCGGCAC CTGTCCTACG AGTTGCATGA TAAAGAAGAC AGTCATAAGT GCGGCGACGA TAGTCATGCC CCGCGCCCAC CGGAAGGAGC TGACTGGGTT GAAGGCTCTC AAGGGCATCG GTCGACGCTC TCCCTTATGC GACTCCTGCA TTAGGAAGCA GCCCAGTAGT AGGTTGAGGC CGTTGAGCAC CGCCGCCGCA AGGAATGGTG CATGCAAGGA GATGGCGCCC AACAGTCCCC CGGCCACGGG GCCTGCCACC ATACCCACGC CGAAACAAGC GCTCATGAGC CCGAAGTGGC GAGCCCGATC TTCCCCATCG GTGATGTCGG CGATATAGGC GCCAGCAACC GCACCTGTGG CGCCGGTGAT GCCGGCCACG ATGCGTCCGG CGTAGAGGAT CGAGATCTCG ATCCCGCGAA AT
Details for MytuD.17946.a.B1.PS38422
| PURIFICATION DATe: | 2/27/2018 |
| CONCENTRATION: | 42.92mg/ml |
| OBSERVED MW: | 76kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | SEC: 20 mM HEPES pH 7.0, 300 mM NaCl,5% glycerol, 1 mM TCEP |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| VIAL COUNT (approx.): | 3 |
| VIAL VOLUME: | 110µl |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MAHHHHHHMS SPDADQTAPE VLRQWQALAE EVREHQFRYY VRDAPIISDA EFDELLRRLE ALEEQHPELR TPDSPTQLVG GAGFATDFEP VDHLERMLSL DNAFTADELA AWAGRIHAEV GDAAHYLCEL KIDGVALSLV YREGRLTRAS TRGDGRTGED VTLNARTIAD VPERLTPGDD YPVPEVLEVR GEVFFRLDDF QALNASLVEE GKAPFANPRN SAAGSLRQKD PAVTARRRLR MICHGLGHVE GFRPATLHQA YLALRAWGLP VSEHTTLATD LAGVRERIDY WGEHRHEVDH EIDGVVVKVD EVALQRRLGS TSRAPRWAIA YKYPPEEAQT KLLDIRVNVG RTGRITPFAF MTPVKVAGST VGQATLHNAS EIKRKGVLIG DTVVIRKAGD VIPEVLGPVV ELRDGSEREF IMPTTCPECG SPLAPEKEGD ADIRCPNARG CPGQLRERVF HVASRNGLDI EVLGYEAGVA LLQAKVIADE GELFALTERD LLRTDLFRTK AGELSANGKR LLVNLDKAKA APLWRVLVAL SIRHVGPTAA RALATEFGSL DAIAAASTDQ LAAVEGVGPT IAAAVTEWFA VDWHREIVDK WRAAGVRMVD ERDESVPRTL AGLTIVVTGS LTGFSRDDAK EAIVARGGKA AGSVSKKTNY VVAGDSPGSK YDKAVELGVP ILDEDGFRRL LADGPASRT
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATGTGAGCT CCCCAGACGC CGATCAGACC GCTCCCGAGG TGTTGCGGCA GTGGCAGGCA CTGGCCGAGG AGGTGCGTGA GCACCAGTTC CGTTATTACG TGCGGGACGC GCCGATCATC AGCGACGCGG AATTCGACGA GCTGCTGCGC CGTCTGGAAG CCCTCGAGGA GCAGCATCCC GAGCTGCGCA CGCCCGATTC GCCGACCCAG CTGGTCGGCG GTGCCGGCTT CGCCACGGAT TTCGAGCCCG TCGACCATCT CGAACGAATG CTCAGCCTCG ACAACGCGTT CACCGCCGAC GAACTCGCCG CCTGGGCCGG CCGCATCCAT GCCGAGGTCG GAGACGCCGC ACATTACCTG TGTGAGCTCA AGATCGACGG CGTCGCGCTG TCTTTGGTCT ACCGCGAGGG ACGGCTGACC CGGGCCTCCA CCCGCGGCGA CGGGCGCACC GGCGAGGACG TCACCCTGAA CGCCCGGACC ATCGCCGACG TTCCCGAACG GCTCACCCCC GGCGACGACT ACCCGGTGCC CGAGGTCCTC GAGGTCCGCG GCGAGGTCTT CTTCCGGCTG GACGACTTCC AGGCGCTCAA CGCCAGCCTC GTCGAGGAGG GCAAGGCGCC GTTCGCCAAC CCCCGCAACA GCGCGGCGGG ATCGCTGCGC CAGAAAGACC CGGCGGTCAC CGCGCGCCGC CGGCTGCGGA TGATCTGCCA CGGGCTGGGC CACGTGGAGG GCTTTCGCCC GGCCACCCTG CATCAGGCAT ACCTGGCGTT GCGGGCATGG GGACTGCCGG TTTCCGAACA CACCACCCTG GCAACCGACC TGGCCGGTGT GCGCGAGCGC ATCGACTACT GGGGCGAGCA CCGCCACGAG GTGGACCACG AAATCGACGG CGTGGTGGTC AAAGTCGACG AGGTGGCGTT GCAGCGCAGG CTGGGTTCCA CGTCGCGGGC GCCGCGCTGG GCCATCGCCT ACAAGTACCC GCCCGAGGAA GCGCAGACCA AGCTGCTCGA CATCCGGGTG AACGTCGGCC GCACCGGGCG GATCACGCCG TTTGCGTTCA TGACGCCGGT GAAGGTGGCC GGGTCGACGG TGGGACAGGC CACCCTGCAC AACGCCTCGG AGATCAAGCG CAAGGGCGTG CTGATCGGCG ACACCGTGGT GATCCGCAAG GCCGGCGACG TGATCCCCGA GGTGCTGGGA CCCGTCGTCG AACTGCGCGA TGGCTCCGAA CGCGAATTCA TCATGCCCAC CACCTGCCCG GAGTGCGGTT CGCCGTTGGC GCCGGAGAAG GAAGGCGACG CCGACATCCG TTGCCCCAAC GCCCGCGGCT GCCCGGGGCA ACTGCGGGAG CGGGTTTTCC ACGTCGCCAG CCGCAACGGC CTAGACATCG AGGTGCTCGG TTACGAGGCG GGTGTGGCGC TCTTGCAGGC GAAGGTGATC GCCGACGAGG GCGAGCTGTT CGCGCTGACC GAGCGGGACT TGCTGCGCAC CGACCTGTTC CGAACCAAGG CAGGCGAACT GTCGGCCAAC GGCAAACGGC TGCTGGTCAA CCTCGACAAG GCCAAGGCGG CACCGCTGTG GCGGGTGCTG GTGGCGCTGT CCATCCGCCA TGTCGGGCCG ACGGCGGCCC GCGCCCTGGC CACCGAGTTC GGCAGCCTTG ACGCCATCGC CGCGGCGTCC ACCGACCAGC TGGCCGCCGT CGAGGGGGTG GGGCCGACCA TTGCCGCCGC GGTCACCGAG TGGTTCGCCG TCGACTGGCA CCGCGAGATC GTCGACAAGT GGCGGGCCGC CGGGGTGCGA ATGGTCGACG AGCGTGACGA GAGTGTGCCA CGCACGCTGG CCGGGCTGAC CATCGTGGTC ACCGGCTCGC TGACCGGTTT CTCCCGCGAC GACGCCAAGG AGGCGATCGT GGCCCGCGGC GGCAAGGCCG CCGGCTCGGT GTCGAAGAAG ACCAACTATG TCGTCGCCGG AGACTCGCCG GGATCCAAAT ACGACAAGGC GGTGGAGTTG GGGGTGCCGA TTCTGGACGA GGATGGGTTC CGGAGACTGC TGGCCGACGG ACCCGCGTCA CGAACGTGAG TAAGATAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AATTCTTGAA GACGAAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC TATGTGGCGC GGTATTATCC CGTGTTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG ACGAGCGTGA CACCACGATG CCTGCAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCTGA TGCGGTATTT TCTCCTTACG CATCTGTGCG GTATTTCACA CCGCATATAT GGTGCACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGTAT ACACTCCGCT ATCGCTACGT GACTGGGTCA TGGCTGCGCC CCGACACCCG CCAACACCCG CTGACGCGCC CTGACGGGCT TGTCTGCTCC CGGCATCCGC TTACAGACAA GCTGTGACCG TCTCCGGGAG CTGCATGTGT CAGAGGTTTT CACCGTCATC ACCGAAACGC GCGAGGCAGC TGCGGTAAAG CTCATCAGCG TGGTCGTGAA GCGATTCACA GATGTCTGCC TGTTCATCCG CGTCCAGCTC GTTGAGTTTC TCCAGAAGCG TTAATGTCTG GCTTCTGATA AAGCGGGCCA TGTTAAGGGC GGTTTTTTCC TGTTTGGTCA CTGATGCCTC CGTGTAAGGG GGATTTCTGT TCATGGGGGT AATGATACCG ATGAAACGAG AGAGGATGCT CACGATACGG GTTACTGATG ATGAACATGC CCGGTTACTG GAACGTTGTG AGGGTAAACA ACTGGCGGTA TGGATGCGGC GGGACCAGAG AAAAATCACT CAGGGTCAAT GCCAGCGCTT CGTTAATACA GATGTAGGTG TTCCACAGGG TAGCCAGCAG CATCCTGCGA TGCAGATCCG GAACATAATG GTGCAGGGCG CTGACTTCCG CGTTTCCAGA CTTTACGAAA CACGGAAACC GAAGACCATT CATGTTGTTG CTCAGGTCGC AGACGTTTTG CAGCAGCAGT CGCTTCACGT TCGCTCGCGT ATCGGTGATT CATTCTGCTA ACCAGTAAGG CAACCCCGCC AGCCTAGCCG GGTCCTCAAC GACAGGAGCA CGATCATGCG CACCCGTGGC CAGGACCCAA CGCTGCCCGA GATGCGCCGC GTGCGGCTGC TGGAGATGGC GGACGCGATG GATATGTTCT GCCAAGGGTT GGTTTGCGCA TTCACAGTTC TCCGCAAGAA TTGATTGGCT CCAATTCTTG GAGTGGTGAA TCCGTTAGCG AGGTGCCGCC GGCTTCCATT CAGGTCGAGG TGGCCCGGCT CCATGCACCG CGACGCAACG CGGGGAGGCA GACAAGGTAT AGGGCGGCGC CTACAATCCA TGCCAACCCG TTCCATGTGC TCGCCGAGGC GGCATAAATC GCCGTGACGA TCAGCGGTCC AGTGATCGAA GTTAGGCTGG TAAGAGCCGC GAGCGATCCT TGAAGCTGTC CCTGATGGTC GTCATCTACC TGCCTGGACA GCATGGCCTG CAACGCGGGC ATCCCGATGC CGCCGGAAGC GAGAAGAATC ATAATGGGGA AGGCCATCCA GCCTCGCGTC GCGAACGCCA GCAAGACGTA GCCCAGCGCG TCGGCCGCCA TGCCGGCGAT AATGGCCTGC TTCTCGCCGA AACGTTTGGT GGCGGGACCA GTGACGAAGG CTTGAGCGAG GGCGTGCAAG ATTCCGAATA CCGCAAGCGA CAGGCCGATC ATCGTCGCGC TCCAGCGAAA GCGGTCCTCG CCGAAAATGA CCCAGAGCGC TGCCGGCACC TGTCCTACGA GTTGCATGAT AAAGAAGACA GTCATAAGTG CGGCGACGAT AGTCATGCCC CGCGCCCACC GGAAGGAGCT GACTGGGTTG AAGGCTCTCA AGGGCATCGG TCGACGCTCT CCCTTATGCG ACTCCTGCAT TAGGAAGCAG CCCAGTAGTA GGTTGAGGCC GTTGAGCACC GCCGCCGCAA GGAATGGTGC ATGCAAGGAG ATGGCGCCCA ACAGTCCCCC GGCCACGGGG CCTGCCACCA TACCCACGCC GAAACAAGCG CTCATGAGCC CGAAGTGGCG AGCCCGATCT TCCCCATCGG TGATGTCGGC GATATAGGCG CCAGCAACCG CACCTGTGGC GCCGGTGATG CCGGCCACGA TGCGTCCGGC GTAGAGGATC GAGATCTCGA TCCCGCGAAA T
Details for MytuD.17946.a.B4.PW38452
| PURIFICATION DATe: | 4/9/2018 |
| CONCENTRATION: | 19.33mg/ml |
| OBSERVED MW: | 60kDa |
| EXPRESSION LEVEL: | Low Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, 0.025% Azide |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| VIAL COUNT (approx.): | 1 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MAHHHHHHTD FEPVDHLERM LSLDNAFTAD ELAAWAGRIH AEVGDAAHYL CELKIDGVAL SLVYREGRLT RASTRGDGRT GEDVTLNART IADVPERLTP GDDYPVPEVL EVRGEVFFRL DDFQALNASL VEEGKAPFAN PRNSAAGSLR QKDPAVTARR RLRMICHGLG HVEGFRPATL HQAYLALRAW GLPVSEHTTL ATDLAGVRER IDYWGEHRHE VDHEIDGVVV KVDEVALQRR LGSTSRAPRW AIAYKYPPEE AQTKLLDIRV NVGRTGRITP FAFMTPVKVA GSTVGQATLH NASEIKRKGV LIGDTVVIRK AGDVIPEVLG PVVELRDGSE REFIMPTTCP ECGSPLAPEK EGDADIRCPN ARGCPGQLRE RVFHVASRNG LDIEVLGYEA GVALLQAKVI ADEGELFALT ERDLLRTDLF RTKAGELSAN GKRLLVNLDK AKAAPLWRVL VALSIRHVGP TAARALATEF GSLDAIAAAS TDQLAAVEGV GPTIAAAVTE WFAVDWHREI VDKWRAAGVR MVDERD
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATACGGATT TCGAGCCCGT CGACCATCTC GAACGAATGC TCAGCCTCGA CAACGCGTTC ACCGCCGACG AACTCGCCGC CTGGGCCGGC CGCATCCATG CCGAGGTCGG AGACGCCGCA CATTACCTGT GTGAGCTCAA GATCGACGGC GTCGCGCTGT CTTTGGTCTA CCGCGAGGGA CGGCTGACCC GGGCCTCCAC CCGCGGCGAC GGGCGCACCG GCGAGGACGT CACCCTGAAC GCCCGGACCA TCGCCGACGT TCCCGAACGG CTCACCCCCG GCGACGACTA CCCGGTGCCC GAGGTCCTCG AGGTCCGCGG CGAGGTCTTC TTCCGGCTGG ACGACTTCCA GGCGCTCAAC GCCAGCCTCG TCGAGGAGGG CAAGGCGCCG TTCGCCAACC CCCGCAACAG CGCGGCGGGA TCGCTGCGCC AGAAAGACCC GGCGGTCACC GCGCGCCGCC GGCTGCGGAT GATCTGCCAC GGGCTGGGCC ACGTGGAGGG CTTTCGCCCG GCCACCCTGC ATCAGGCATA CCTGGCGTTG CGGGCATGGG GACTGCCGGT TTCCGAACAC ACCACCCTGG CAACCGACCT GGCCGGTGTG CGCGAGCGCA TCGACTACTG GGGCGAGCAC CGCCACGAGG TGGACCACGA AATCGACGGC GTGGTGGTCA AAGTCGACGA GGTGGCGTTG CAGCGCAGGC TGGGTTCCAC GTCGCGGGCG CCGCGCTGGG CCATCGCCTA CAAGTACCCG CCCGAGGAAG CGCAGACCAA GCTGCTCGAC ATCCGGGTGA ACGTCGGCCG CACCGGGCGG ATCACGCCGT TTGCGTTCAT GACGCCGGTG AAGGTGGCCG GGTCGACGGT GGGACAGGCC ACCCTGCACA ACGCCTCGGA GATCAAGCGC AAGGGCGTGC TGATCGGCGA CACCGTGGTG ATCCGCAAGG CCGGCGACGT GATCCCCGAG GTGCTGGGAC CCGTCGTCGA ACTGCGCGAT GGCTCCGAAC GCGAATTCAT CATGCCCACC ACCTGCCCGG AGTGCGGTTC GCCGTTGGCG CCGGAGAAGG AAGGCGACGC CGACATCCGT TGCCCCAACG CCCGCGGCTG CCCGGGGCAA CTGCGGGAGC GGGTTTTCCA CGTCGCCAGC CGCAACGGCC TAGACATCGA GGTGCTCGGT TACGAGGCGG GTGTGGCGCT CTTGCAGGCG AAGGTGATCG CCGACGAGGG CGAGCTGTTC GCGCTGACCG AGCGGGACTT GCTGCGCACC GACCTGTTCC GAACCAAGGC AGGCGAACTG TCGGCCAACG GCAAACGGCT GCTGGTCAAC CTCGACAAGG CCAAGGCGGC ACCGCTGTGG CGGGTGCTGG TGGCGCTGTC CATCCGCCAT GTCGGGCCGA CGGCGGCCCG CGCCCTGGCC ACCGAGTTCG GCAGCCTTGA CGCCATCGCC GCGGCGTCCA CCGACCAGCT GGCCGCCGTC GAGGGGGTGG GGCCGACCAT TGCCGCCGCG GTCACCGAGT GGTTCGCCGT CGACTGGCAC CGCGAGATCG TCGACAAGTG GCGGGCCGCC GGGGTGCGAA TGGTCGACGA GCGTGACTGA GTAAGATAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAATTCTTGA AGACGAAAGG GCCTCGTGAT ACGCCTATTT TTATAGGTTA ATGTCATGAT AATAATGGTT TCTTAGACGT CAGGTGGCAC TTTTCGGGGA AATGTGCGCG GAACCCCTAT TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT AACCCTGATA AATGCTTCAA TAATATTGAA AAAGGAAGAG TATGAGTATT CAACATTTCC GTGTCGCCCT TATTCCCTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT CACCCAGAAA CGCTGGTGAA AGTAAAAGAT GCTGAAGATC AGTTGGGTGC ACGAGTGGGT TACATCGAAC TGGATCTCAA CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC CGAAGAACGT TTTCCAATGA TGAGCACTTT TAAAGTTCTG CTATGTGGCG CGGTATTATC CCGTGTTGAC GCCGGGCAAG AGCAACTCGG TCGCCGCATA CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA CAGAAAAGCA TCTTACGGAT GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA TGAGTGATAA CACTGCGGCC AACTTACTTC TGACAACGAT CGGAGGACCG AAGGAGCTAA CCGCTTTTTT GCACAACATG GGGGATCATG TAACTCGCCT TGATCGTTGG GAACCGGAGC TGAATGAAGC CATACCAAAC GACGAGCGTG ACACCACGAT GCCTGCAGCA ATGGCAACAA CGTTGCGCAA ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG ACTGGATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGGTC TCGCGGTATC ATTGCAGCAC TGGGGCCAGA TGGTAAGCCC TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA CTATGGATGA ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT AACTGTCAGA CCAAGTTTAC TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTC CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC GAACGACCGA GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA AGAGCGCCTG ATGCGGTATT TTCTCCTTAC GCATCTGTGC GGTATTTCAC ACCGCATATA TGGTGCACTC TCAGTACAAT CTGCTCTGAT GCCGCATAGT TAAGCCAGTA TACACTCCGC TATCGCTACG TGACTGGGTC ATGGCTGCGC CCCGACACCC GCCAACACCC GCTGACGCGC CCTGACGGGC TTGTCTGCTC CCGGCATCCG CTTACAGACA AGCTGTGACC GTCTCCGGGA GCTGCATGTG TCAGAGGTTT TCACCGTCAT CACCGAAACG CGCGAGGCAG CTGCGGTAAA GCTCATCAGC GTGGTCGTGA AGCGATTCAC AGATGTCTGC CTGTTCATCC GCGTCCAGCT CGTTGAGTTT CTCCAGAAGC GTTAATGTCT GGCTTCTGAT AAAGCGGGCC ATGTTAAGGG CGGTTTTTTC CTGTTTGGTC ACTGATGCCT CCGTGTAAGG GGGATTTCTG TTCATGGGGG TAATGATACC GATGAAACGA GAGAGGATGC TCACGATACG GGTTACTGAT GATGAACATG CCCGGTTACT GGAACGTTGT GAGGGTAAAC AACTGGCGGT ATGGATGCGG CGGGACCAGA GAAAAATCAC TCAGGGTCAA TGCCAGCGCT TCGTTAATAC AGATGTAGGT GTTCCACAGG GTAGCCAGCA GCATCCTGCG ATGCAGATCC GGAACATAAT GGTGCAGGGC GCTGACTTCC GCGTTTCCAG ACTTTACGAA ACACGGAAAC CGAAGACCAT TCATGTTGTT GCTCAGGTCG CAGACGTTTT GCAGCAGCAG TCGCTTCACG TTCGCTCGCG TATCGGTGAT TCATTCTGCT AACCAGTAAG GCAACCCCGC CAGCCTAGCC GGGTCCTCAA CGACAGGAGC ACGATCATGC GCACCCGTGG CCAGGACCCA ACGCTGCCCG AGATGCGCCG CGTGCGGCTG CTGGAGATGG CGGACGCGAT GGATATGTTC TGCCAAGGGT TGGTTTGCGC ATTCACAGTT CTCCGCAAGA ATTGATTGGC TCCAATTCTT GGAGTGGTGA ATCCGTTAGC GAGGTGCCGC CGGCTTCCAT TCAGGTCGAG GTGGCCCGGC TCCATGCACC GCGACGCAAC GCGGGGAGGC AGACAAGGTA TAGGGCGGCG CCTACAATCC ATGCCAACCC GTTCCATGTG CTCGCCGAGG CGGCATAAAT CGCCGTGACG ATCAGCGGTC CAGTGATCGA AGTTAGGCTG GTAAGAGCCG CGAGCGATCC TTGAAGCTGT CCCTGATGGT CGTCATCTAC CTGCCTGGAC AGCATGGCCT GCAACGCGGG CATCCCGATG CCGCCGGAAG CGAGAAGAAT CATAATGGGG AAGGCCATCC AGCCTCGCGT CGCGAACGCC AGCAAGACGT AGCCCAGCGC GTCGGCCGCC ATGCCGGCGA TAATGGCCTG CTTCTCGCCG AAACGTTTGG TGGCGGGACC AGTGACGAAG GCTTGAGCGA GGGCGTGCAA GATTCCGAAT ACCGCAAGCG ACAGGCCGAT CATCGTCGCG CTCCAGCGAA AGCGGTCCTC GCCGAAAATG ACCCAGAGCG CTGCCGGCAC CTGTCCTACG AGTTGCATGA TAAAGAAGAC AGTCATAAGT GCGGCGACGA TAGTCATGCC CCGCGCCCAC CGGAAGGAGC TGACTGGGTT GAAGGCTCTC AAGGGCATCG GTCGACGCTC TCCCTTATGC GACTCCTGCA TTAGGAAGCA GCCCAGTAGT AGGTTGAGGC CGTTGAGCAC CGCCGCCGCA AGGAATGGTG CATGCAAGGA GATGGCGCCC AACAGTCCCC CGGCCACGGG GCCTGCCACC ATACCCACGC CGAAACAAGC GCTCATGAGC CCGAAGTGGC GAGCCCGATC TTCCCCATCG GTGATGTCGG CGATATAGGC GCCAGCAACC GCACCTGTGG CGCCGGTGAT GCCGGCCACG ATGCGTCCGG CGTAGAGGAT CGAGATCTCG ATCCCGCGAA AT