MyleA.17946.a

PROBABLE DNA LIGASE [NAD DEPENDENT] LIGA (POLYDEOXYRIBONUCLEOTIDE SYNTHASE [NAD+])

CENTER ID: MyleA.17946.a
ORGANISM: Mycobacterium leprae Br4923
ASSOCIATED DISEASE: Hansen's disease, leprosy
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyleA.17946.a.A1.GE34209 full length 1 694
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
External Resources
RESOURCE REFERENCE ID
OrthoMCL: OG5_131384
PATRIC ID: fig|561304.4.peg.3224
RefSeq: YP_002503804.1
UniProt: B8ZS27
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSSPEADPIG PEVLQQWRKL TEEVREHQFR YYVRDAPIIS DAEFDALLDR LTVLEEQHPE LCTPDSPTQL VGGAGFMTDF VATEHLERMF SLDNAFSGDE LNAWVTRIRA EIGNDAHYLC ELKIDGVALS LVYRDGRLTR ATTRGDGRTG EDVTLNARTI EDVPERLTVS DIYPLPEVLE VRGEVFFRVV DFQALNTSLV EEGKNPFANP RNSAAGSLRQ KDPAVTAHRK LRMICHGLGH IEGFRPATQH QAYLALRDWG LPVSEHTTLV NDIAGVQGCI AYWAEHRHEV DHEIDGIVVK IDEVTLQRRL GSTSRAPRWA IAYKYLPEEA QTKLLDIRVN VGRTGRVTPF AFMTPVKVAG STVGQATLHN PSEVKRKGVL IGDTVVIRKA GDVIPEVLGP VVDLRDGSER EFVMPTTCPE CGTTLAPEKE GDADIRCPNA RSCPGQLRER VFHVASRSAL DIQGLGYEAG VALLAAQVIT SEGDLFTLTE KALLRTELFR NKAGELSANG KRLLVNVDKA KTAPLWRVLV ALSIRHVGPT AARALATEFG SVDAILAASP EQLAAVEGVG TTIAAAVTEW FTVDWHRVIV NKWRAAGVRM VDERDTSVLP TCEGLTIVVT GSLAGFSRDD AKEAIVARGG KVAGSVSKKI AYVVVGDLPG YKYDKAVELG VPILNEDGFR LLLEQGPPVQ QVVD
NT Sequence
gtgagttccc ccgaagctga ccctatcgga cccgaggtgt tgcagcagtg gcgaaagctg accgaagagg tgcgtgaaca ccagttccgt tactatgtgc gtgacgcgcc gatcatctct gacgcggagt ttgatgctct gctggacagg ctcactgtgc tggaggaaca gcatcccgag ctgtgcacgc ccgattcacc tacccagctg gtcggcggtg ctggcttcat gactgatttc gtggcgaccg agcatctcga acggatgttc agcctcgaca acgccttcag tggggatgaa ctcaatgcct gggtcactcg catccgcgca gagatcggta atgacgccca ttatttgtgt gagctcaaga tcgacggtgt cgcattgtcg ttggtctacc gggatggacg cctgacccgc gccacgactc ggggcgatgg tcgcaccggc gaagacgtca cgctgaacgc gcggaccatc gaagatgtgc cggaacggct gaccgtcagc gatatttacc cgctgccaga ggttcttgag gtccgcggcg aggtgttctt ccgagtggtg gactttcaag cgcttaacac gagcctcgtt gaggagggca agaatccgtt cgctaaccca cgaaacagcg cggcgggctc gctgcgccag aaggatccgg cggtcacggc tcaccgtaaa ctgcgaatga tctgtcatgg actggggcac atcgaaggct ttcgtcccgc tacccagcat caggcttact tagctttacg ggactggggg ttgccggtgt ccgagcatac gacgctggtc aacgacatag ctggcgtcca gggatgcatc gcatactggg ccgaacatcg tcacgaggtg gaccatgaaa tcgacgggat agtggtcaaa atcgacgagg ttacgttgca gcgcaggctg ggttccacct cgcgagctcc acgatgggcc atcgcttaca agtacctacc cgaggaggcg cagaccaagc tgctcgacat ccgggtgaac gtcggccgca ccggtcgggt tacgccattc gcgttcatga cgcccgtgaa agttgccggg tcgacggtcg ggcaggcgac cttgcacaac ccttctgagg tcaaacgcaa aggtgtgctg atcggtgaca ccgtggtgat ccgcaaggcc ggcgacgtga ttcccgaagt tttgggacct gtggttgact tgcgcgacgg gtccgaacgt gaatttgtga tgcccacaac gtgtcccgag tgcggtacta cgttagcgcc agaaaaggaa ggcgatgccg acatccgttg ccccaatgcc aggagctgcc cggggcaact gcgagagcga gtgtttcacg tcgctagccg cagcgctctg gacattcagg ggttgggtta cgaggccggt gtggcgttgc tggctgcgca agtgatcact agcgagggcg acctgttcac cctcaccgag aaggccttgt tgcgcaccga gcttttccgc aacaaggcag gggagttgtc ggcgaatggc aaacggttgc tagtcaacgt cgacaaagcc aaaactgcac cgctgtggcg agtgctggtg gcgttgtcga tccggcatgt cgggccgacg gctgcgcgag ccttggccac cgagttcggc agcgttgacg caatcttggc cgcgtcccct gaacaactgg ccgctgtcga gggtgtaggg accaccatcg cggccgcggt taccgagtgg ttcaccgtcg actggcatcg cgtgattgtc aataagtggc gggctgccgg ggtgcggatg gtcgatgagc gtgacaccag cgtgctgccc acatgcgaag ggttgaccat cgtggtcact ggttctttag cagggttttc ccgtgacgat gccaaggagg cgatcgtggc gcgcggaggc aaggtcgctg gttcggtatc gaagaagatc gcctacgtgg tcgtaggcga cttgccggga tacaaatatg acaaggcggt cgagctcggg gtgccgatcc tcaacgagga cgggtttcgc ctgttgcttg agcagggacc tccggtgcag caagtcgttg actag
Details for MyleA.17946.a.A1.GE34209
HARVESTED ON: 12/6/2011
SEQUENCED ON: 12/8/2011
EXPECTED MW: 78kDa
OBSERVED MW: 79kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 94
PERCENT COVERAGE: 44
Validated AA Sequence
MAHHHHHXXX XLEAQTQGPG SMVSSPEADP IGPEVLQQWR KLTEEVREHQ FRYYVRDAPI ISDAEFDALL DRLTVLEEQH PELCTPDSPT QLVGGAGFMT DFVATEHLER MFSLDNAFSG DELNAWVTRI RAEIGNDAHY LCELKIDGVA LSLVYRDGRL TRATTRGDGR TGEDVTLNAR TIEDVPERLT VSDIYPLPEV LEVRGEVFFR VVDFQALNTS LVEEGKNPFA NPRNSAAGSL RQKDPAVTAH RKLRMICHGL GHIXGFRPAT QHQAYLALRD WGLPVSEHTT LVNDIAGVQG HRILXXHRHX XXMKSXG
Validated NT Sequence
attttgttta ctttaagaag gagatatacc atggctcatc accatcacca tcnnnnnnnn nccctggaag ctcagaccca gggtcctggt tcgatggtga gttcccccga agctgaccct atcggacccg aggtgttgca gcagtggcga aagctgaccg aagaggtgcg tgaacaccag ttccgttact atgtgcgtga cgcgccgatc atctctgacg cggagtttga tgctctgctg gacaggctca ctgtgctgga ggaacagcat cccgagctgt gcacgcccga ttcacctacc cagctggtcg gcggtgctgg cttcatgact gatttcgtgg cgaccgagca tctcgaacgg atgttcagcc tcgacaacgc cttcagtggg gatgaactca atgcctgggt cactcgcatc cgcgcagaga tcggtaatga cgcccattat ttgtgtgagc tcaagatcga cggtgtcgca ttgtcgttgg tctaccggga tggacgcctg acccgcgcca cgactcgggg cgatggtcgc accggcgaag acgtcacgct gaacgcgcgg accatcgaag atgtgccgga acggctgacc gtcagcgata tttacccgct gccagaggtt cttgaggtcc gcggcgaggt gttcttccga gtggtggact ttcaagcgct taacacgagc ctcgttgagg agggcaagaa tccgttcgct aacccacgaa acagcgcggc gggctcgctg cgccagaagg atccggcggt cacggctcac cgtaaactgc gaatgatctg tcatggactg gggcacatcg anggctttcg tcccgctacc cagcatcagg cttacttagc tttacgggac tgggggttgc cggtgtccga gcatacnacg ctggtcaacg acatagctgg cgtccagggg catcgcatac tnnnnganca tcgtcacgan gngnnnatga aatcganggg ntagnngnca aatcgacnag nncnttgcan cgcnnntggn nnnctnncnn cncnnnangg nnntcnntnn agtncnancc nnngnngnnn nnannnntnn nnnnnnnnng nnnanntnnn nnnnnnnnnn nnnnnncntn annnccnnnn nnnnnnnnnn nnnngnng
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVSSPEADP IGPEVLQQWR KLTEEVREHQ FRYYVRDAPI ISDAEFDALL DRLTVLEEQH PELCTPDSPT QLVGGAGFMT DFVATEHLER MFSLDNAFSG DELNAWVTRI RAEIGNDAHY LCELKIDGVA LSLVYRDGRL TRATTRGDGR TGEDVTLNAR TIEDVPERLT VSDIYPLPEV LEVRGEVFFR VVDFQALNTS LVEEGKNPFA NPRNSAAGSL RQKDPAVTAH RKLRMICHGL GHIEGFRPAT QHQAYLALRD WGLPVSEHTT LVNDIAGVQG CIAYWAEHRH EVDHEIDGIV VKIDEVTLQR RLGSTSRAPR WAIAYKYLPE EAQTKLLDIR VNVGRTGRVT PFAFMTPVKV AGSTVGQATL HNPSEVKRKG VLIGDTVVIR KAGDVIPEVL GPVVDLRDGS EREFVMPTTC PECGTTLAPE KEGDADIRCP NARSCPGQLR ERVFHVASRS ALDIQGLGYE AGVALLAAQV ITSEGDLFTL TEKALLRTEL FRNKAGELSA NGKRLLVNVD KAKTAPLWRV LVALSIRHVG PTAARALATE FGSVDAILAA SPEQLAAVEG VGTTIAAAVT EWFTVDWHRV IVNKWRAAGV RMVDERDTSV LPTCEGLTIV VTGSLAGFSR DDAKEAIVAR GGKVAGSVSK KIAYVVVGDL PGYKYDKAVE LGVPILNEDG FRLLLEQGPP VQQVVD
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgagttcc cccgaagctg accctatcgg acccgaggtg ttgcagcagt ggcgaaagct gaccgaagag gtgcgtgaac accagttccg ttactatgtg cgtgacgcgc cgatcatctc tgacgcggag tttgatgctc tgctggacag gctcactgtg ctggaggaac agcatcccga gctgtgcacg cccgattcac ctacccagct ggtcggcggt gctggcttca tgactgattt cgtggcgacc gagcatctcg aacggatgtt cagcctcgac aacgccttca gtggggatga actcaatgcc tgggtcactc gcatccgcgc agagatcggt aatgacgccc attatttgtg tgagctcaag atcgacggtg tcgcattgtc gttggtctac cgggatggac gcctgacccg cgccacgact cggggcgatg gtcgcaccgg cgaagacgtc acgctgaacg cgcggaccat cgaagatgtg ccggaacggc tgaccgtcag cgatatttac ccgctgccag aggttcttga ggtccgcggc gaggtgttct tccgagtggt ggactttcaa gcgcttaaca cgagcctcgt tgaggagggc aagaatccgt tcgctaaccc acgaaacagc gcggcgggct cgctgcgcca gaaggatccg gcggtcacgg ctcaccgtaa actgcgaatg atctgtcatg gactggggca catcgaaggc tttcgtcccg ctacccagca tcaggcttac ttagctttac gggactgggg gttgccggtg tccgagcata cgacgctggt caacgacata gctggcgtcc agggatgcat cgcatactgg gccgaacatc gtcacgaggt ggaccatgaa atcgacggga tagtggtcaa aatcgacgag gttacgttgc agcgcaggct gggttccacc tcgcgagctc cacgatgggc catcgcttac aagtacctac ccgaggaggc gcagaccaag ctgctcgaca tccgggtgaa cgtcggccgc accggtcggg ttacgccatt cgcgttcatg acgcccgtga aagttgccgg gtcgacggtc gggcaggcga ccttgcacaa cccttctgag gtcaaacgca aaggtgtgct gatcggtgac accgtggtga tccgcaaggc cggcgacgtg attcccgaag ttttgggacc tgtggttgac ttgcgcgacg ggtccgaacg tgaatttgtg atgcccacaa cgtgtcccga gtgcggtact acgttagcgc cagaaaagga aggcgatgcc gacatccgtt gccccaatgc caggagctgc ccggggcaac tgcgagagcg agtgtttcac gtcgctagcc gcagcgctct ggacattcag gggttgggtt acgaggccgg tgtggcgttg ctggctgcgc aagtgatcac tagcgagggc gacctgttca ccctcaccga gaaggccttg ttgcgcaccg agcttttccg caacaaggca ggggagttgt cggcgaatgg caaacggttg ctagtcaacg tcgacaaagc caaaactgca ccgctgtggc gagtgctggt ggcgttgtcg atccggcatg tcgggccgac ggctgcgcga gccttggcca ccgagttcgg cagcgttgac gcaatcttgg ccgcgtcccc tgaacaactg gccgctgtcg agggtgtagg gaccaccatc gcggccgcgg ttaccgagtg gttcaccgtc gactggcatc gcgtgattgt caataagtgg cgggctgccg gggtgcggat ggtcgatgag cgtgacacca gcgtgctgcc cacatgcgaa gggttgacca tcgtggtcac tggttcttta gcagggtttt cccgtgacga tgccaaggag gcgatcgtgg cgcgcggagg caaggtcgct ggttcggtat cgaagaagat cgcctacgtg gtcgtaggcg acttgccggg atacaaatat gacaaggcgg tcgagctcgg ggtgccgatc ctcaacgagg acgggtttcg cctgttgctt gagcagggac ctccggtgca gcaagtcgtt gactaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa