SSGCID: Target Page

MytuD.17258.a

helicase-nuclease AddA Rv3201c

CENTER ID:	MytuD.17258.a
ORGANISM:	Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE:	Tuberculosis
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MytuD.17258.a.A3.GE30104	UvrD-like helicase C-terminal domain		357	670
MytuD.17258.a.A4.GE30105	C-terminal domain (HHPred)		901	1070

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|83332.12.peg.3579
RefSeq:	NP_217717.1
TubercuList:	Rv3201c
MTB Network Portal:	Rv3201c
UniProt:	O53347

Orthologues for Rv3201c

Species	% Identity	% Coverage	Clones	Proteins	Structures
marinum	79	99	0	0	0
smegmatis	69	99	2	1	0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTQTAAPARY SPAELACALG LFPPTAEQAA VIAAPPGPLV VIAGAGAGKT ETMAARVVWL VANGYAEPGQ VLGLTFTRKA AGQLLRRVRS RLARLAGIGL GCGDPAACAP VVSTYHAFAG SLLRDYGLLL PLEPDTRLLS ETELWQLAFD VVSGYDGVLC TDKSPAAVTS IVVRLWGQLG EHLVDTRALR DTHVELERLV HALPAGRYQR DRGPSQWLLR MLATQTQRAE LVPLLDALGE RMHAGKVMDF AMQMASAARL AATSPQVGQD LRRRYRVVLL DEYQDTGHAQ RVVLSSLFGG GVDDGLALTA VGDPIQSIYG WRGASATNLP RFTTDFPLSD GTPAPVLELL TSWRNPPQAL RVANGISAEA RRRSVAVRAL RPRPDAPPGA VRCALLPDVQ AEREWIADHL RMRYQRAEAD GVKPPTAAVL VRRNADAAAI ADTLRARGIP AEVVGLAGLL SIPEVAEVVA MLRLVADPTA GAAAMRVLTG PRWRLGARDL AALWRRALTL SGESPSTASP ESIAMAASAD ADNPCLADAI SDPGSAEGYS VAGYGRIGAL AGELSALRGR LGHSLPDLVA EVRRVLGVDC EVRASAPVSG GWAGPEHLDA FADVVAGYAE RASARSSEAS VAGLLAYLDV AEVVENGLPP AELTVACDRV QVLTVHAAKG LEWQVVAVAH LSRGVFPSTV SRSSWLTDPA ELPPLLRGDR ASAGAHGIPV LDTSAVADRK QLSDKISEHR RLLDRRRVDE ERRLLYVAVT RAEDTLLVSG HHWGPTGTKP RGPSEFLCEL KDIIDRSAAA GDPCGVVEQW ASAPAGDERN PLCDNAIEAV WPADPLAARR GDVERGAALV AAAMSADLPG STTDIDHPPR PGDAPWSTDV DALLAERAHA ARGAPARGLP NHLSVSSLVE LVGDPVGARQ RLMCRLPKRP DPHAWLGDAF HAWVQQFYGA ELLFDLGDLP GAADREVGDP EELAALQRAF TASSWAARTP AAVEVPFEMP IGDTVVRGRI DAVFVDPDGG ATVVDWKTGK PPHGPAAMRQ AAVQLAVYRL AWAALRGCPT SSVRTAFYYV RSGITVVPDE LPAPGELAML LTDCAGRRSD T

NT Sequence

atgacccaaa ccgcggcacc ggcacgctac agtccagctg aactagcctg cgcgctagga cttttcccgc ccaccgctga gcaggccgcg gtaatcgccg cacctcccgg cccgctggtc gtcatcgcgg gagccggcgc cggaaaaacc gaaacgatgg ctgcccgggt ggtgtggctg gttgccaacg gctacgcgga acccggccag gtgctgggat tgacgttcac ccgcaaggcc gcgggccagc tgttgcgccg cgttcggtcc cgtctggcca ggttggccgg tatcggcctc ggctgcggcg acccggcggc ctgcgctccg gtagtcagca cctaccacgc ctttgccggc tcgctactac gcgactacgg cctgctgcta ccgctggaac ccgataccag gttgctgagc gagaccgagc tgtggcagct ggcattcgat gtggtcagcg ggtacgacgg cgtgctgtgc accgacaaga gcccggcggc ggtcacctcg atcgtggtgc ggttatgggg ccagcttggc gagcatctgg tcgacacccg cgcgcttcgc gacacccacg tcgagctgga acggctggtc cacgctctgc cggcgggccg ctatcagcgt gaccgcggcc ccagccagtg gctgctgcgg atgctggcca cccagaccca gcgcgcggag ttggtgccgt tgcttgacgc gttgggtgag cggatgcacg ccggcaaagt gatggatttc gccatgcaga tggcctcggc cgcacggttg gcggcgacct ctccccaagt tggccaggat ctgcggaggc gctaccgggt ggtgttactg gacgaatacc aggacacggg gcacgcgcag cgcgttgtgt tgtcgtcgct gtttggcggc ggcgttgatg acgggttggc gctgacggct gtcggtgacc cgattcagtc gatctacggg tggcgcggcg cttcggcgac gaacctgccc cggttcacca ccgacttccc cttatcggac ggcacccccg cgccggtgct ggagctgctg accagctggc gcaacccgcc gcaagcgctg cgcgtggcca atggcatatc ggcggaggcg cggcggcgct cggttgcggt gcgcgcgttg cggccgcgtc cggatgcccc gcctggagcc gtccgctgcg cgttgcttcc cgacgtgcag gccgaacgcg aatggatcgc cgaccacttg cggatgcgtt accagcgggc agaagcggac ggtgtcaagc cgcccaccgc ggcggtgctg gtacgccgca acgccgacgc cgccgctatc gctgataccc tgcgggcccg cggaatcccg gctgaagttg tcgggctggc cggcctactg tccatccccg aggtcgccga ggtggtggcc atgctgcgcc tggttgccga cccaacggcc ggggcggccg cgatgcgggt gctgactggc ccacggtggc ggctcggcgc ccgggacctc gccgcgctgt ggcggcgtgc gctgacgctg agtggcgaat caccgtcaac ggcgtcgccc gaatcgatcg cgatggcggc cagcgcggac gccgacaatc cgtgtctggc cgacgccatc agcgatccag gttccgccga ggggtattcg gtggcgggat atgggcgcat cggcgccttg gccggtgaac tgagcgcgct gcgcggtcgc ctcggtcatt ccctgcccga cctagtagcc gaggtgcgcc gcgtgcttgg cgtcgattgc gaggtgcggg cctcggcccc ggtctcaggt ggctgggccg gacccgagca ccttgatgcg ttcgccgacg tggtcgcggg gtacgccgaa cgggccagcg ctaggtccag cgaggcgtcg gttgcgggcc tgctggctta tttggacgtc gccgaggtgg tcgagaacgg tttgccgcct gccgagttga ccgtcgcctg cgaccgggtc caggtgctga ccgtgcacgc cgccaagggc ttggaatggc aggtggtggc ggtggcacac ctgtcgcgcg gagtgtttcc gtcgacggtg tcgcgcagca gctggcttac cgaccccgcc gaactaccgc cgctgctgcg tggggatcgc gcctcggccg gcgcgcacgg catccccgta ctggacacgt cagctgtcgc tgaccgaaaa cagttgtcgg acaagatctc cgaacatcgt cgcctgctcg atcggcggcg cgttgacgag gagcgtcgac tgttgtacgt ggccgttacc cgggctgagg acactctgct ggtgtccggc caccattggg ggcccaccgg gacaaagccg cgcgggccat cggaattcct gtgcgaactc aaggacatca ttgaccgttc ggccgctgcc ggtgatcctt gcggagtcgt cgagcagtgg gcgtcggcgc ccgccggcga tgagcgaaac ccattgtgtg acaacgctat cgaggcggtt tggcccgctg atccgttggc cgcacgtcgc ggcgatgtgg agcggggcgc ggcgctggtg gcggcggcca tgtcggccga cttgcccgga agcaccaccg acatcgatca tcccccgcgc ccgggcgatg ccccctggtc cactgacgtc gatgcactgt tggctgaacg tgcgcacgcg gcgcggggag cacctgcccg tggtctgccg aaccatttgt cggtcagcag tttggtggag ctggtcggcg acccggtcgg tgcgcggcag cggctgatgt gtcggctgcc aaaacgcccg gacccgcacg cgtggttggg cgatgcattt cacgcctggg ttcagcaatt ttacggtgct gaactgctgt ttgacctggg tgacctgcca ggtgcagcgg accgcgaggt gggcgatcct gaggagttgg ccgcgttgca gagggcgttc accgcatcat cgtgggcggc tcgcaccccg gcggcggtcg aagtgccgtt cgaaatgccg atcggcgata ccgtggtgcg cggccgcatc gacgcggtgt tcgtcgaccc cgacggcggt gccaccgtgg tggactggaa gaccggcaag ccgccgcacg ggccggcagc catgcggcag gccgctgtcc agctcgctgt ctatcgactg gcctgggccg cattgcgggg atgtccgaca tcctcggtgc gtaccgcctt ctattacgtg cgcagcggca tcacggtcgt ccccgacgag ttacccgctc ccggcgagtt ggccatgcta ctgaccgact gcgccggtcg gcggtcggac acctga

Details for MytuD.17258.a.A3.GE30104

HARVESTED ON:	8/15/2010
SEQUENCED ON:	8/25/2010
EXPECTED MW:	34kDa
OBSERVED MW:	35kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	93

Validated AA Sequence

MPQALRVANG ISAEARRRSV AVRALRPRPD APPGAVRCAL LPDVQAEREW IADHLRMRYQ RAEADGVKPP TAAVLVRRNA DAAAIADTLR ARGIPAEVVG LAGLLSIPEV AEVVAMLRLV ADPTAGAAAM RVLTGPRWRL GARDLAALWR RALTLSGESP STASPESIAM AASADADNPC LADAISDPGS AEGYSVAGYG RIGALAGELS ALRGRLGHSL PDLVAEVRRV LGVDCEVRAS APVSGGWAGP EHLDAFADVV AGYAERASAR SSEASVAGLL AYLDVAEVVE NGLPPAELTV ACDRVQVLTV HAA

Validated NT Sequence

tggcggcgtg cacggtcagc acctggaccc ggtcgcaggc gacggtcaac tcggcaggcg gcaaaccgtt ctcgaccacc tcggcgacgt ccaaataagc cagcaggccc gcaaccgacg cctcgctgga cctagcgctg gcccgttcgg cgtaccccgc gaccacgtcg gcgaacgcat caaggtgctc gggtccggcc cagccacctg agaccggggc cgaggcccgc acctcgcaat cgacgccaag cacgcggcgc acctcggcta ctaggtcggg cagggaatga ccgaggcgac cgcgcagcgc gctcagttca ccggccaagg cgccgatgcg cccatatccc gccaccgaat acccctcggc ggaacctgga tcgctgatgg cgtcggccag acacggattg tcggcgtccg cgctggccgc catcgcgatc gattcgggcg acgccgttga cggtgattcg ccactcagcg tcagcgcacg ccgccacagc gcggcgaggt cccgggcgcc gagccgccac cgtgggccag tcagcacccg catcgcggcc gccccggccg ttgggtcggc aaccaggcgc agcatggcca ccacctcggc gacctcgggg atggacagta ggccggccag cccgacaact tcagccggga ttccgcgggc ccgcagggta tcagcgatag cggcggcgtc ggcgttgcgg cgtaccagca ccgccgcggt gggcggcttg acaccgtccg cttctgcccg ctggtaacgc atccgcaagt ggtcggcgat ccattcgcgt tcggcctgca cgtcgggaag caacgcgcag cggacggctc caggcggggc atccggacgc ggccgcaacg cgcgcaccgc aaccgagcgc cgccgcgcct ccgccgatat gccattggcc acgcgcagcg cttgcggcat cgaaccagga ccctgggtct gagcttccag

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMPQALRVAN GISAEARRRS VAVRALRPRP DAPPGAVRCA LLPDVQAERE WIADHLRMRY QRAEADGVKP PTAAVLVRRN ADAAAIADTL RARGIPAEVV GLAGLLSIPE VAEVVAMLRL VADPTAGAAA MRVLTGPRWR LGARDLAALW RRALTLSGES PSTASPESIA MAASADADNP CLADAISDPG SAEGYSVAGY GRIGALAGEL SALRGRLGHS LPDLVAEVRR VLGVDCEVRA SAPVSGGWAG PEHLDAFADV VAGYAERASA RSSEASVAGL LAYLDVAEVV ENGLPPAELT VACDRVQVLT VHAAKG

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gccgcaagcg ctgcgcgtgg ccaatggcat atcggcggag gcgcggcggc gctcggttgc ggtgcgcgcg ttgcggccgc gtccggatgc cccgcctgga gccgtccgct gcgcgttgct tcccgacgtg caggccgaac gcgaatggat cgccgaccac ttgcggatgc gttaccagcg ggcagaagcg gacggtgtca agccgcccac cgcggcggtg ctggtacgcc gcaacgccga cgccgccgct atcgctgata ccctgcgggc ccgcggaatc ccggctgaag ttgtcgggct ggccggccta ctgtccatcc ccgaggtcgc cgaggtggtg gccatgctgc gcctggttgc cgacccaacg gccggggcgg ccgcgatgcg ggtgctgact ggcccacggt ggcggctcgg cgcccgggac ctcgccgcgc tgtggcggcg tgcgctgacg ctgagtggcg aatcaccgtc aacggcgtcg cccgaatcga tcgcgatggc ggccagcgcg gacgccgaca atccgtgtct ggccgacgcc atcagcgatc caggttccgc cgaggggtat tcggtggcgg gatatgggcg catcggcgcc ttggccggtg aactgagcgc gctgcgcggt cgcctcggtc attccctgcc cgacctagta gccgaggtgc gccgcgtgct tggcgtcgat tgcgaggtgc gggcctcggc cccggtctca ggtggctggg ccggacccga gcaccttgat gcgttcgccg acgtggtcgc ggggtacgcc gaacgggcca gcgctaggtc cagcgaggcg tcggttgcgg gcctgctggc ttatttggac gtcgccgagg tggtcgagaa cggtttgccg cctgccgagt tgaccgtcgc ctgcgaccgg gtccaggtgc tgaccgtgca cgccgccaag ggcaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa

Details for MytuD.17258.a.A4.GE30105

HARVESTED ON:	8/15/2010
SEQUENCED ON:	8/25/2010
EXPECTED MW:	20kDa
OBSERVED MW:	20kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMNHLSVSSL VELVGDPVGA RQRLMCRLPK RPDPHAWLGD AFHAWVQQFY GAELLFDLGD LPGAADREVG DPEELAALQR AFTASSWAAR TPAAVEVPFE MPIGDTVVRG RIDAVFVDPD GGATVVDWKT GKPPHGPAAM RQAAVQLAVY RLAWAALRGC PTSSVRTAFY YV

Validated NT Sequence

ttttgtttac tttaagaagg agatatacca tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatgaacca tttgtcggtc agcagtttgg tggagctggt cggcgacccg gtcggtgcgc ggcagcggct gatgtgtcgg ctgccaaaac gcccggaccc gcacgcgtgg ttgggcgatg catttcacgc ctgggttcag caattttacg gtgctgaact gctgtttgac ctgggtgacc tgccaggtgc agcggaccgc gaggtgggcg atcctgagga gttggccgcg ttgcagaggg cgttcaccgc atcatcgtgg gcggctcgca ccccggcggc ggtcgaagtg ccgttcgaaa tgccgatcgg cgataccgtg gtgcgcggcc gcatcgacgc ggtgttcgtc gaccccgacg gcggtgccac cgtggtggac tggaagaccg gcaagccgcc gcacgggccg gcagccatgc ggcaggccgc tgtccagctc gctgtctatc gactggcctg ggccgcattg cggggatgtc cgacatcctc ggtgcgtacc gccttctatt acgtgtaata aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct ganttggctg ctgccaccgc nnannnnnnn nnnnnnnna

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaaccatttg tcggtcagca gtttggtgga gctggtcggc gacccggtcg gtgcgcggca gcggctgatg tgtcggctgc caaaacgccc ggacccgcac gcgtggttgg gcgatgcatt tcacgcctgg gttcagcaat tttacggtgc tgaactgctg tttgacctgg gtgacctgcc aggtgcagcg gaccgcgagg tgggcgatcc tgaggagttg gccgcgttgc agagggcgtt caccgcatca tcgtgggcgg ctcgcacccc ggcggcggtc gaagtgccgt tcgaaatgcc gatcggcgat accgtggtgc gcggccgcat cgacgcggtg ttcgtcgacc ccgacggcgg tgccaccgtg gtggactgga agaccggcaa gccgccgcac gggccggcag ccatgcggca ggccgctgtc cagctcgctg tctatcgact ggcctgggcc gcattgcggg gatgtccgac atcctcggtg cgtaccgcct tctattacgt gaaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa