MysmA.17258.a

helicase-nuclease AddA (AdnB)

CENTER ID:	MysmA.17258.a
ORGANISM:	Mycobacterium smegmatis ATCC 700084 / mc(2)155
ASSOCIATED DISEASE:
CURRENT STATUS:	crystallized
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MysmA.17258.a.A2.GE30118	UvrD-like helicase ATP-binding domain		17	352
MysmA.17258.a.A4.GE30120	C-terminal domain		881	1074

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MysmA.17258.a.A4.PS00785	C-terminal domain		881	1074

External Resources

RESOURCE	REFERENCE ID
OrthoMCL:	OG5_239164
PATRIC ID:	fig\|246196.19.peg.1922
RefSeq:	YP_886308.1
UniProt:	A0QTS0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTQVASPVVQ ARYSPVELSA ALGLFPPTDE QAAVIAAPPG PLVVIAGAGA GKTETMAARV VWLVANGFAT PSQVLGLTFT RKAAGQLLRR VRTRLARLAG AGLAPGSGAS DESATVSTYH AFAGTLLREH GLLLPVEPDT RLLSETELWQ LAYDVVCAHP GHLDTEKTPA AVTAMVLRLS GALAEHLVDT DQLRDTHVEL ERLVHTLPAG PYQRDRGPSQ WLLRMLATQT ERTELVPLID ALHQRMRAEK VMDFGMQMAA AARLAARFPQ VGEQLRQRFR VVLLDEYQDT GHAQRIALSS LFGGGADDGL ALTAVGDPIQ SIYGWRGASA TNLPRFTTDF PYSDGTPAPT LELRTSWRNP PSTLHVANAV SEEARRRSVA VRALRPRPDA EPGTIRCALL NNVAAERDWV ADHLARAYHG AIGRGEAAPT AAVLVRRNAD AAPMAEALTA RGVPVEVVGV AGLLAVPEVA DLVAMLRLIA DPTAGSAVMR ILTGPRWRFG ARDIAALWRR AVELDDRPKG ELGTADIVAQ AAPDADTACV ADAICDPGDA ERYSPAGYER IVALGRELTM LRAHLGHPLP ELVAEVRRVL GLDAEARAAR PVAAGWAGTE NLDRFSDLVS DFAGHAGASV SALLAYLDAA VEVENGLAPA ELTVSHDRVQ ILTVHAAKGL EWQVVAVPHL SARVFPSTTQ ARTWLTDASD LPPLLRGDRA TESEIGVPVL DTSDIYDRKI LSDKISDHKK SLDQRRVDEE RRLLYVAITR AEDTLLLSGH HWGATESKPR GPSEFLCELK TILEEATAAG TPCGEIEHWA PDPAPGETNP LRDQVVEALW PPVASADDHV HRGAQLVAAA MAGEVSAEAD QEGWAADVDA LLAERERPPQ QEDTELPGQL SVSTLVELSR DPKAALTRLR RRLPQRPDPH ALLGTTFHEW VQRYFHAERL FDLDDLPGAV DSDSGRAVEE SLAELQDAFV KSPWAARTPV EVEVPFDMVL GETVVRGRID AVFAEPDGTT MVLDWKTGDP PETPEAKEHA AVQLAVYRLA WAAMRGCPPE SVRAAFHYVR SGQTVIPETL PGAEELVKLL AAAPTETAEE ADRIT

NT Sequence

gtgacgcagg tagcatcgcc cgtcgtgcag gcgcgataca gcccggtcga gctctcggcg gccctcgggc ttttcccgcc caccgatgaa caggccgcgg tgatcgccgc gccgccaggg ccgctggtcg tgatcgccgg tgccggtgcg ggcaagaccg agaccatggc tgcgcgcgtg gtgtggttgg tggccaacgg gttcgccacg ccgtcgcagg tgctgggtct gacattcacg cgcaaggccg ccggtcagct gctgcgccgg gtccgcacgc ggctcgcgcg tctggccggt gccggactgg caccgggctc gggtgcgtcc gacgaatcgg cgaccgtcag cacctaccac gcgttcgcgg gaaccctgct gcgtgagcac ggcctattgc tgcccgtcga acccgacacc cggctgctca gcgagaccga gctgtggcag ctggcctacg acgtggtgtg tgcccacccc ggacacctcg acaccgagaa gaccccggcc gccgtcaccg cgatggtgtt gcggctctcg ggcgcactcg ccgagcatct cgtggacacc gaccagttgc gggacacaca cgtcgaactg gagcgactgg tccacaccct gcccgccggg ccgtatcagc gtgaccgcgg gcccagccag tggctgctgc gcatgctcgc cacccaaacc gagcgcaccg aactggtgcc gctcatcgac gcgctgcacc agcgcatgcg cgccgagaag gtcatggact tcggcatgca gatggccgcg gccgcacgct tggcggcgcg gttcccgcag gtgggcgagc aactgcggca gcgctttcgc gtggtgctgc tcgacgagta ccaggacacc ggccacgcgc agcgcatcgc gttgtcgtcg ttgttcggcg gcggtgccga cgacggtctc gcgctcaccg cggtgggcga tccgatccag tcgatctacg gctggcgcgg ggcctcggcg accaacctgc cgcggttcac caccgatttc ccgtactccg acggcacccc cgcgccgacg ctggagctgc ggaccagttg gcgcaacccg cccagcacgc tgcacgtggc caacgcggtc tccgaggaag cgcgccgccg ctcggtggcc gtccgcgcac tgcggccccg gcccgacgcc gaacctggca cgatccgctg cgcgttgctg aacaacgtcg ccgccgaacg cgactgggtc gcagaccatc tcgcccgtgc ctaccacggc gccatcggac gcggcgaggc cgcgcccacc gcggcggtgc tcgtgcggcg caacgccgac gccgcgccga tggcagaggc gctcaccgcc cgcggcgttc ccgtggaggt cgtcggtgtc gccgggctgc tcgccgtgcc cgaggtcgcc gacctggtcg cgatgctgcg gctgatcgcc gacccgaccg ccggttcggc ggtcatgcgg atcctcaccg gtccgcgctg gcggttcggt gcccgtgaca tcgccgcgct gtggcggcgt gccgtcgaac tcgacgaccg gccgaaaggc gaactgggca cggcggacat cgtcgcgcag gcagcacccg acgccgacac cgcgtgcgtg gccgacgcga tctgcgaccc gggagatgcc gaacggtatt cgcctgccgg gtacgagcgc atcgtcgcgc tgggacgtga actcaccatg cttcgcgcgc acctgggcca tccgctgccg gaactggtcg ccgaggtgcg ccgcgtgctg ggcctggacg ccgaggcccg cgccgcacgc cccgtcgcgg ccgggtgggc aggcaccgag aacctcgacc ggttcagcga tctggtgtcc gatttcgccg ggcacgcagg cgcatcggta tcggcgttgc tcgcctacct cgatgccgcg gtggaggtcg agaacggtct ggccccagcg gaactcacgg tgtcgcacga ccgcgtgcag atcctcaccg tccacgcggc caagggactg gaatggcagg tggtggcggt gccgcacctg agtgcccggg tgttcccgtc gaccacccag gcgcggacat ggctgaccga tgcctccgac ctgccgccgc tgctgcgcgg tgaccgtgcc accgagtccg agatcggcgt cccggtgctg gacacgtctg acatctacga ccggaaaatc ctgtccgaca agatttctga ccacaagaag agcctggatc agcggcgggt ggatgaagag cgtcggctgc tgtacgtcgc gatcacccgt gcggaggaca cgctgctgct gtccgggcat cactggggcg ccaccgagag caaaccgcgc gggccctcgg agttcctgtg cgagctgaag accatcctgg aagaggccac ggcagcgggt acgccgtgcg gtgagatcga gcactgggca ccggatcccg caccgggcga gaccaatcca ctgcgcgatc aggtggtcga ggcgctgtgg ccgccggtgg cctccgccga tgaccatgtg caccgcggtg cgcaactggt cgccgccgcg atggccggtg aggtgagcgc cgaggccgat caggagggct gggccgccga cgtcgacgca ctgctggccg aacgcgagcg cccaccgcaa caggaggaca ccgaactgcc gggtcagctg tcggtgagca ccctggtcga actcagccgt gacccgaagg ccgcgctgac gcggctgcgg cgccgcctac cgcaacggcc tgatccacat gccctgctgg gcaccacatt ccacgagtgg gtgcagcgct acttccatgc cgagcgcctc ttcgacctcg acgaccttcc cggtgcggtc gacagtgaca gcggacgcgc cgtcgaggag agcctggccg aactgcagga cgcgttcgtc aaatccccgt gggccgcgcg caccccggtg gaggtcgagg tgccgttcga catggtgctc ggcgagaccg tcgtgcgggg ccgcatcgac gcggtgttcg ccgaaccgga cggcaccacc atggttctcg actggaagac cggcgacccg ccggaaactc cggaggccaa ggaacatgcg gcggttcagc tcgcggtgta ccggctggcg tgggcggcaa tgcgcggctg cccgcccgag tcggtgcgcg cggcgttcca ctatgtccgt tccggtcaga ccgtcatccc cgaaaccctg cccggagcag aagaactggt gaaactgctc gccgcagcgc cgaccgaaac agccgaggaa gccgaccgga tcacgtga

Details for MysmA.17258.a.A2.GE30118

HARVESTED ON:	8/15/2010
SEQUENCED ON:	8/25/2010
EXPECTED MW:	38kDa
OBSERVED MW:	38kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	94

Validated AA Sequence

MELSAALGLF PPTDEQAAVI AAPPGPLVVI AGAGAGKTET MAARVVWLVA NGFATPSQVL GLTFTRKAAG QLLRRVRTRL ARLAGAGLAP GSGASDESAT VSTYHAFAGT LLREHGLLLP VEPDTRLLSE TELWQLAYDV VCAHPGHLDT EKTPAAVTAM VLRLSGALAE HLVDTDQLRD THVELERLVH TLPAGPYQRD RGPSQWLLRM LATQTERTEL VPLIDALHQR MRAEKVMDFG MQMAAAARLA ARFPQVGEQL RQRFRVVLLD EYQDTGHAQR IALSSLFGGG ADDGLALTAV GDPIQSIYGW RGASATNLPR FTTDFPYSDG TPAPTLE

Validated NT Sequence

tattactcca gcgtcggcgc gggggtgccg tcggagtacg ggaaatcggt ggtgaaccgc ggcaggttgg tcgccgaggc cccgcgccag ccgtagatcg actggatcgg atcgcccacc gcggtgagcg cgagaccgtc gtcggcaccg ccgccgaaca acgacgacaa cgcgatgcgc tgcgcgtggc cggtgtcctg gtactcgtcg agcagcacca cgcgaaagcg ctgccgcagt tgctcgccca cctgcgggaa ccgcgccgcc aagcgtgcgg ccgcggccat ctgcatgccg aagtccatga ccttctcggc gcgcatgcgc tggtgcagcg cgtcgatgag cggcaccagt tcggtgcgct cggtttgggt ggcgagcatg cgcagcagcc actggctggg cccgcggtca cgctgatacg gcccggcggg cagggtgtgg accagtcgct ccagttcgac gtgtgtgtcc cgcaactggt cggtgtccac gagatgctcg gcgagtgcgc ccgagagccg caacaccatc gcggtgacgg cggccggggt cttctcggtg tcgaggtgtc cggggtgggc acacaccacg tcgtaggcca gctgccacag ctcggtctcg ctgagcagcc gggtgtcggg ttcgacgggc agcaataggc cgtgctcacg cagcagggtt cccgcgaacg cgtggtaggt gctgacggtc gccgattcgt cggacgcacc cgagcccggt gccagtccgg caccggccag acgcgcgagc cgcgtgcgga cccggcgcag cagctgaccg gcggccttgc gcgtgaatgt cagacccagc acctgcgacg gcgtggcgaa cccgttggcc accaaccaca ccacgcgcgc agccatggtc tcggtcttgc ccgcaccggc accggcgatc acgaccagcg gccctggcgg cgcggcgatc accgcggcct gttcatcggt gggcgggaaa agcccgaggg ccgccgagag ctccatcgaa ccaggaccct gggtctgagc ttcca

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMELSAALGL FPPTDEQAAV IAAPPGPLVV IAGAGAGKTE TMAARVVWLV ANGFATPSQV LGLTFTRKAA GQLLRRVRTR LARLAGAGLA PGSGASDESA TVSTYHAFAG TLLREHGLLL PVEPDTRLLS ETELWQLAYD VVCAHPGHLD TEKTPAAVTA MVLRLSGALA EHLVDTDQLR DTHVELERLV HTLPAGPYQR DRGPSQWLLR MLATQTERTE LVPLIDALHQ RMRAEKVMDF GMQMAAAARL AARFPQVGEQ LRQRFRVVLL DEYQDTGHAQ RIALSSLFGG GADDGLALTA VGDPIQSIYG WRGASATNLP RFTTDFPYSD GTPAPTLE

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggagctctcg gcggccctcg ggcttttccc gcccaccgat gaacaggccg cggtgatcgc cgcgccgcca gggccgctgg tcgtgatcgc cggtgccggt gcgggcaaga ccgagaccat ggctgcgcgc gtggtgtggt tggtggccaa cgggttcgcc acgccgtcgc aggtgctggg tctgacattc acgcgcaagg ccgccggtca gctgctgcgc cgggtccgca cgcggctcgc gcgtctggcc ggtgccggac tggcaccggg ctcgggtgcg tccgacgaat cggcgaccgt cagcacctac cacgcgttcg cgggaaccct gctgcgtgag cacggcctat tgctgcccgt cgaacccgac acccggctgc tcagcgagac cgagctgtgg cagctggcct acgacgtggt gtgtgcccac cccggacacc tcgacaccga gaagaccccg gccgccgtca ccgcgatggt gttgcggctc tcgggcgcac tcgccgagca tctcgtggac accgaccagt tgcgggacac acacgtcgaa ctggagcgac tggtccacac cctgcccgcc gggccgtatc agcgtgaccg cgggcccagc cagtggctgc tgcgcatgct cgccacccaa accgagcgca ccgaactggt gccgctcatc gacgcgctgc accagcgcat gcgcgccgag aaggtcatgg acttcggcat gcagatggcc gcggccgcac gcttggcggc gcggttcccg caggtgggcg agcaactgcg gcagcgcttt cgcgtggtgc tgctcgacga gtaccaggac accggccacg cgcagcgcat cgcgttgtcg tcgttgttcg gcggcggtgc cgacgacggt ctcgcgctca ccgcggtggg cgatccgatc cagtcgatct acggctggcg cggggcctcg gcgaccaacc tgccgcggtt caccaccgat ttcccgtact ccgacggcac ccccgcgccg acgctggaga aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a

Details for MysmA.17258.a.A4.GE30120

HARVESTED ON:	8/15/2010
SEQUENCED ON:	8/25/2010
EXPECTED MW:	23kDa
OBSERVED MW:	26kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMQEDTELPG QLSVSTLVEL SRDPKAALTR LRRRLPQRPD PHALLGTTFH EWVQRYFHAE RLFDLDDLPG AVDSDSGRAV EESLAELQDA FVKSPWAART PVEVEVPFDM VLGETVVRGR IDAVFAEPDG TTMVLDWKTG DPPETPEAKE HAAVQLAVYR LAWAAMRGCP PESVRAAFHY VRSGQTVIPE TLPGAE

Validated NT Sequence

ttattattct gctccgggca gggtttcggg gatgacggtc tgaccggaac ggacatagtg gaacgccgcg cgcaccgact cgggcgggca gccgcgcatt gccgcccacg ccagccggta caccgcgagc tgaaccgccg catgttcctt ggcctccgga gtttccggcg ggtcgccggt cttccagtcg agaaccatgg tggtgccgtc cggttcggcg aacaccgcgt cgatgcggcc ccgcacgacg gtctcgccga gcaccatgtc gaacggcacc tcgacctcca ccggggtgcg cgcggcccac ggggatttga cgaacgcgtc ctgcagttcg gccaggctct cctcgacggc gcgtccgctg tcactgtcga ccgcaccggg aaggtcgtcg aggtcgaaga ggcgctcggc atggaagtag cgctgcaccc actcgtggaa tgtggtgccc agcagggcat gtggatcagg ccgttgcggt aggcggcgcc gcagccgcgt cagcgcggcc ttcgggtcac ggctgagttc gaccagggtg ctcaccgaca gctgacccgg cagttcggtg tcctcctgca tcgaaccagg accctgggtc tgagcttcca gggtacccat atgatggtga tggtgatgag ccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcaggaggac accgaactgc cgggtcagct gtcggtgagc accctggtcg aactcagccg tgacccgaag gccgcgctga cgcggctgcg gcgccgccta ccgcaacggc ctgatccaca tgccctgctg ggcaccacat tccacgagtg ggtgcagcgc tacttccatg ccgagcgcct cttcgacctc gacgaccttc ccggtgcggt cgacagtgac agcggacgcg ccgtcgagga gagcctggcc gaactgcagg acgcgttcgt caaatccccg tgggccgcgc gcaccccggt ggaggtcgag gtgccgttcg acatggtgct cggcgagacc gtcgtgcggg gccgcatcga cgcggtgttc gccgaaccgg acggcaccac catggttctc gactggaaga ccggcgaccc gccggaaact ccggaggcca aggaacatgc ggcggttcag ctcgcggtgt accggctggc gtgggcggca atgcgcggct gcccgcccga gtcggtgcgc gcggcgttcc actatgtccg ttccggtcag accgtcatcc ccgaaaccct gcccggagca gaaaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa

Details for MysmA.17258.a.A4.PS00785

PURIFICATION DATe:	9/28/2010
CONCENTRATION:	41.8mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	6
VIAL VOLUME:	data unavailable
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GCAGGAGGAC ACCGAACTGC CGGGTCAGCT GTCGGTGAGC ACCCTGGTCG AACTCAGCCG TGACCCGAAG GCCGCGCTGA CGCGGCTGCG GCGCCGCCTA CCGCAACGGC CTGATCCACA TGCCCTGCTG GGCACCACAT TCCACGAGTG GGTGCAGCGC TACTTCCATG CCGAGCGCCT CTTCGACCTC GACGACCTTC CCGGTGCGGT CGACAGTGAC AGCGGACGCG CCGTCGAGGA GAGCCTGGCC GAACTGCAGG ACGCGTTCGT CAAATCCCCG TGGGCCGCGC GCACCCCGGT GGAGGTCGAG GTGCCGTTCG ACATGGTGCT CGGCGAGACC GTCGTGCGGG GCCGCATCGA CGCGGTGTTC GCCGAACCGG ACGGCACCAC CATGGTTCTC GACTGGAAGA CCGGCGACCC GCCGGAAACT CCGGAGGCCA AGGAACATGC GGCGGTTCAG CTCGCGGTGT ACCGGCTGGC GTGGGCGGCA ATGCGCGGCT GCCCGCCCGA GTCGGTGCGC GCGGCGTTCC ACTATGTCCG TTCCGGTCAG ACCGTCATCC CCGAAACCCT GCCCGGAGCA GAAAAACAGC ACGAACAAGT TCTGCAGCCA AGCTTCTCGA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAA