MytuD.17102.a

Putative uncharacterized protein Rv3683

CENTER ID: MytuD.17102.a
ORGANISM: Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE: Tuberculosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MytuD.17102.a.A2.GE27757 mature protein 30 319
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
External Resources
RESOURCE REFERENCE ID
OrthoMCL: OG5_131163
PATRIC ID: fig|83332.12.peg.4104
RefSeq: NP_218200.1
TubercuList: Rv3683
MTB Network Portal: Rv3683
UniProt: I6X827
Orthologues for Rv3683
Species % Identity % Coverage Clones Proteins Structures
ulcerans 91 98 0 0 0
paratuberculosis 87 99 1 0 0
leprae 85 94 0 0 0
smegmatis 80 98 0 0 0
abscessus 71 100 1 0 0
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAAVLPTLIR TGAVALGSAI AGIGYAALVE RNAFVLREVT MPVLTPGSTP LRVLHISDLH MLPNQHRKQA WLRELASWEP DLVVNTGDNL AHPKAVPAVV QTLSDLLSRP GVFVFGSNDY FGPRLKNPMN YLTSPDHRVR GAALPWQDLR AAFTERGWLD LTHTRREFEV AGLHIAAAGV DDPHIDRDRY DTIAGPASPA ANLRLGLTHS PEPRVLDRFA ADGYQLVLAG HTHGGQLCLP LYGALVTNCG LDRSRAKGAS HWGANMRLHV SAGIGTSPFA PVRFCCRPEA TLLTLIATPM GGRDSSSNLG RSQPTVSVR
NT Sequence
atggctgctg tcttgcccac cttgatccgc accggcgccg tggcgttggg ctcggccatc gccgggattg gttacgctgc gctggtcgag cgcaatgcat tcgtcctgcg cgaggtgacc atgccagtct tgactccggg ctccacaccg ctgcgggtgc tgcacatcag cgatctgcat atgctgccca accagcaccg caaacaggcc tggctgcgcg agctcgccag ctgggagccg gatctggtcg tcaacaccgg tgacaacctg gctcacccca aggcggtgcc cgccgtcgtc caaaccctga gcgatctgct gtcccggccg ggtgtcttcg tgttcggcag caacgactac tttgggccgc gcctgaagaa cccaatgaac tatctgacca gcccggatca ccgcgtccgc ggagcagcgc tgccctggca ggatctgcgg gcggcgttca ccgaacgtgg gtggctcgac ctaacccata cccgccgcga gttcgaagtt gccggtctgc acatcgccgc tgcgggcgtc gacgacccgc atatcgaccg agaccgctac gacaccatcg ccggcccggc cagcccggcc gccaacctgc ggctggggct cacccattca ccggagccgc gggtgttgga ccgcttcgcc gccgatggtt accagttggt gctggccggc cacacccacg gcgggcagct gtgcctgccg ttgtacgggg cgctggtcac taactgcggt ctggaccgct cccgggccaa aggagcgtca cactggggtg caaacatgcg gctgcacgtc tccgccggga tcggcacttc gccgtttgcg ccggtgagat tctgctgccg gcccgaagca accctgctga cgttgatcgc gaccccaatg ggcgggcgcg attcgagcag caacctgggc cgctcacagc cgacagtgtc ggtgcgttga
Details for MytuD.17102.a.A2.GE27757
HARVESTED ON: 11/16/2009
SEQUENCED ON: 11/23/2009
EXPECTED MW: 34kDa
OBSERVED MW: 34kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMERNAFVLR EVTMPVLTPG STPLRVLHIS DLHMLPNQHR KQAWLRELAS WEPDLVVNTG DNLAHPKAVP AVVQTLSDLL SRPGVFVFGS NDYFGPRLKN PMNYLTSPDH RVRGAALPWQ DLRAAFTERG WLDLTHTRRE FEVAGLHIAA AGVDDPHIDR DRYDTIAGPA SPAANLRLGL THSPEPRVLD RFAADGYQLV LAGHTHGGQL CLPLYGALVT NCGLDRSRAK GASHWGANMR LHVSAGIGTS PFAPVRFCCR PEATLLTLIA TPMGGRDSSS NLGRSQPTVS VR
Validated NT Sequence
ttattaacgc accgacactg tcggctgtga gcggcccagg ttgctgctcg aatcgcgccc gcccattggg gtcgcgatca acgtcagcag ggttgcttcg ggccggcagc agaatctcac cggcgcaaac ggcgaagtgc cgatcccggc ggagacgtgc agccgcatgt ttgcacccca gtgtgacgct cctttggccc gggagcggtc cagaccgcag ttagtgacca gcgccccgta caacggcagg cacagctgcc cgccgtgggt gtggccggcc agcaccaact ggtaaccatc ggcggcgaag cggtccaaca cccgcggctc cggtgaatgg gtgagcccca gccgcaggtt ggcggccggg ctggccgggc cggcgatggt gtcgtagcgg tctcggtcga tatgcgggtc gtcgacgccc gcagcggcga tgtgcagacc ggcaacttcg aactcgcggc gggtatgggt taggtcgagc cacccacgtt cggtgaacgc cgcccgcaga tcctgccagg gcagcgctgc tccgcggacg cggtgatccg ggctggtcag atagttcatt gggttcttca ggcgcggccc aaagtagtcg ttgctgccga acacgaagac acccggccgg gacagcagat cgctcagggt ttggacgacg gcgggcaccg ccttggggtg agccaggttg tcaccggtgt tgacgaccag atccggctcc cagctggcga gctcgcgcag ccaggcctgt ttgcggtgct ggttgggcag catatgcaga tcgctgatgt gcagcacccg cagcggtgtg gagcccggag tcaagactgg catggtcacc tcgcgcagga cgaatgcatt gcgctccatc gaaccaggac cctgggtctg agcttccagg gtacccatat gatggtgatg gtgatgagcc at
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMERNAFVLR EVTMPVLTPG STPLRVLHIS DLHMLPNQHR KQAWLRELAS WEPDLVVNTG DNLAHPKAVP AVVQTLSDLL SRPGVFVFGS NDYFGPRLKN PMNYLTSPDH RVRGAALPWQ DLRAAFTERG WLDLTHTRRE FEVAGLHIAA AGVDDPHIDR DRYDTIAGPA SPAANLRLGL THSPEPRVLD RFAADGYQLV LAGHTHGGQL CLPLYGALVT NCGLDRSRAK GASHWGANMR LHVSAGIGTS PFAPVRFCCR PEATLLTLIA TPMGGRDSSS NLGRSQPTVS VR
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggagcgcaat gcattcgtcc tgcgcgaggt gaccatgcca gtcttgactc cgggctccac accgctgcgg gtgctgcaca tcagcgatct gcatatgctg cccaaccagc accgcaaaca ggcctggctg cgcgagctcg ccagctggga gccggatctg gtcgtcaaca ccggtgacaa cctggctcac cccaaggcgg tgcccgccgt cgtccaaacc ctgagcgatc tgctgtcccg gccgggtgtc ttcgtgttcg gcagcaacga ctactttggg ccgcgcctga agaacccaat gaactatctg accagcccgg atcaccgcgt ccgcggagca gcgctgccct ggcaggatct gcgggcggcg ttcaccgaac gtgggtggct cgacctaacc catacccgcc gcgagttcga agttgccggt ctgcacatcg ccgctgcggg cgtcgacgac ccgcatatcg accgagaccg ctacgacacc atcgccggcc cggccagccc ggccgccaac ctgcggctgg ggctcaccca ttcaccggag ccgcgggtgt tggaccgctt cgccgccgat ggttaccagt tggtgctggc cggccacacc cacggcgggc agctgtgcct gccgttgtac ggggcgctgg tcactaactg cggtctggac cgctcccggg ccaaaggagc gtcacactgg ggtgcaaaca tgcggctgca cgtctccgcc gggatcggca cttcgccgtt tgcgccggtg agattctgct gccggcccga agcaaccctg ctgacgttga tcgcgacccc aatgggcggg cgcgattcga gcagcaacct gggccgctca cagccgacag tgtcggtgcg taaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa