MyabA.17102.a

Putative uncharacterized protein

CENTER ID:	MyabA.17102.a
ORGANISM:	Mycobacterium abscessus ATCC 19977 / DSM 44196
ASSOCIATED DISEASE:	Skin abscess
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyabA.17102.a.A1.GE34907	Full length( MyabA.17102.a )		1	320

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
OrthoMCL:	OG5_131163
PATRIC ID:	fig\|36809.5.peg.441
RefSeq:	YP_001701160.1
UniProt:	B1MFV6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSTSLNTVIK RAAAYSAGSA ALGIGYASII ERNAFTVREA TMDVLDPGAS PLRVLHISDI HMRPGQRLKQ AWLRELAQWQ PDLVINTGDN LAHPKAVSSV VQSLGDLLSI PGLFVFGSND YFGPRLKNPA KYLYKSHERK HGEPLPWQDL RAAFTERGWH DMTHTRREIE VGGVRIAAAG VDDPHLHRDR YDTIAGMPNP LATLRLGLTH SPEPRVLDRF AEDGYQLVMA GHTHGGQLCL PLYGAIVTNC ELDRSRVKGP SQWGSHMQLH VSAGIGTSPF APMRFFCRPE ATLLTLVPAR TSGHGADAES GDRSPSATVQ

NT Sequence

ATGAGCACGT CGTTGAACAC GGTTATCAAG CGGGCGGCTG CCTACTCGGC GGGCTCGGCC GCGCTTGGCA TCGGGTACGC GTCGATCATC GAACGCAACG CGTTCACGGT CCGCGAGGCG ACGATGGATG TGCTCGATCC CGGCGCGTCT CCACTGCGGG TGCTGCACAT CAGTGATATC CACATGCGGC CCGGTCAGCG TCTCAAGCAG GCTTGGCTGC GTGAGTTGGC GCAGTGGCAA CCGGACCTGG TGATCAACAC CGGCGACAAT CTCGCACACC CCAAGGCGGT CTCGTCGGTG GTGCAATCCC TGGGTGATTT GCTTTCCATC CCCGGGCTTT TCGTCTTCGG AAGCAACGAC TACTTCGGCC CCCGCCTCAA GAACCCGGCC AAATATCTGT ACAAGAGCCA TGAGCGCAAG CATGGCGAGC CGCTGCCGTG GCAGGACCTG CGCGCTGCGT TCACCGAGCG TGGTTGGCAC GACATGACCC ACACCCGCCG CGAGATCGAG GTCGGCGGTG TCCGCATCGC GGCTGCCGGG GTGGATGATC CGCATCTGCA CCGGGACCGA TACGACACCA TCGCCGGCAT GCCGAACCCC CTGGCGACAT TGCGACTGGG CCTGACTCAC TCCCCCGAAC CGCGGGTGCT GGATCGCTTC GCCGAGGACG GCTATCAGCT GGTGATGGCC GGACACACCC ACGGCGGTCA GCTGTGCCTG CCGCTCTACG GCGCGATCGT CACCAACTGC GAGCTGGACC GCTCTCGGGT GAAAGGCCCC TCGCAGTGGG GTTCACACAT GCAGCTGCAC GTGTCGGCGG GCATCGGAAC GTCGCCGTTC GCGCCCATGC GATTCTTCTG CCGCCCGGAG GCGACACTGC TGACCCTGGT TCCGGCTCGC ACCAGCGGCC ACGGGGCCGA CGCCGAATCC GGCGATCGCT CGCCCAGCGC CACCGTGCAG

Details for MyabA.17102.a.A1.GE34907

HARVESTED ON:	4/17/2012
SEQUENCED ON:	4/17/2012
EXPECTED MW:	37kDa
OBSERVED MW:	37kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	93
PERCENT COVERAGE:	105

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMSTSLNTVI KRAAAYSAGS AALGIGYASI IERNAFTVRE ATMDVLDPGA SPLRVLHISD IHMRPGQRLK QAWLRELAQW QPDLVINTGD NLAHPKAVSS VVQSLGDLLS IPGLFVFGSN DYFGPRLKNP AKYLYKSHER KHGEPLPWQD LRAAFTERGW HDMTHTRREI EVGGVRIAAA GVDDPHLHRD RYDTIAGMPN PLATLRLGLT HSPEPRVLDR FAEDGYQLVM AGHTHGGQLC LXLYGAIVTN CELDRSRVKG PSQWGSHMQL HVSAGIGTSP FXPMXFFCRP EXXLLXLVXX XTSGHGAXXX SXXXXXXXXX AXIXXXXXXX XXXXXXXXQK K

Validated NT Sequence

anattttnnn nnnnttaaga aggaganata ccatggctca tcaccatcac catcatatgg gtaccctgga agctcagacc cagggtcctg gttcgatgag cacgtcgttg aacacggtta tcaagcgggc ggctgcctac tcggcgggct cggccgcgct tggcatcggg tacgcgtcga tcatcgaacg caacgcgttc acggtccgcg aggcgacgat ggatgtgctc gatcccggcg cgtctccact gcgggtgctg cacatcagtg atatccacat gcggcccggt cagcgtctca agcaggcttg gctgcgtgag ttggcgcagt ggcaaccgga cctggtgatc aacaccggcg acaatctcgc acaccccaag gcggtctcgt cggtggtgca atccctgggt gatttgcttt ccatccccgg gcttttcgtc ttcggaagca acgactactt cggcccccgc ctcaagaacc cggccaaata tctgtacaag agccatgagc gcaagcatgg cgagccgctg ccgtggcagg acctgcgcgc tgcgttcacc gagcgtggtt ggcacgacat gacccacacc cgccgcgaga tcgaggtcgg cggtgtccgc atcgcggctg ccggggtgga tgatccgcat ctgcaccggg accgatacga caccatcgcc ggcatgccga accccctggc gacattgcga ctgggcctga ctcactcccc cgaaccgcgg gtgctggatc gcttcgccga ggacggctat cagctggtga tggccggaca cacccacggc ggtcagctgt gcctgncgct ctacggcgcg atcgtcacca actgcgagct ggaccgctct cgggtgaaag gnccctcgca gtggggttca cacatgcagc tgcacgtgtc ggcgggcatc ggaacgtcgc cgttcncgcc catgcnattc ttctgccgcc cggaggnnan nctgctganc ctggttcnnn nnnnnaccag cggccacggg gccnannccn aatcnnnnna tnnnnncgnc cnncnccnnc nngcantaat aaannncnnn annnantnnn cancatnnnn nnnnnnnnnn nnnncccaaa aaaaaa

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMSTSLNTVI KRAAAYSAGS AALGIGYASI IERNAFTVRE ATMDVLDPGA SPLRVLHISD IHMRPGQRLK QAWLRELAQW QPDLVINTGD NLAHPKAVSS VVQSLGDLLS IPGLFVFGSN DYFGPRLKNP AKYLYKSHER KHGEPLPWQD LRAAFTERGW HDMTHTRREI EVGGVRIAAA GVDDPHLHRD RYDTIAGMPN PLATLRLGLT HSPEPRVLDR FAEDGYQLVM AGHTHGGQLC LPLYGAIVTN CELDRSRVKG PSQWGSHMQL HVSAGIGTSP FAPMRFFCRP EATLLTLVPA RTSGHGADAE SGDRSPSATV Q

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gagcacgtcg ttgaacacgg ttatcaagcg ggcggctgcc tactcggcgg gctcggccgc gcttggcatc gggtacgcgt cgatcatcga acgcaacgcg ttcacggtcc gcgaggcgac gatggatgtg ctcgatcccg gcgcgtctcc actgcgggtg ctgcacatca gtgatatcca catgcggccc ggtcagcgtc tcaagcaggc ttggctgcgt gagttggcgc agtggcaacc ggacctggtg atcaacaccg gcgacaatct cgcacacccc aaggcggtct cgtcggtggt gcaatccctg ggtgatttgc tttccatccc cgggcttttc gtcttcggaa gcaacgacta cttcggcccc cgcctcaaga acccggccaa atatctgtac aagagccatg agcgcaagca tggcgagccg ctgccgtggc aggacctgcg cgctgcgttc accgagcgtg gttggcacga catgacccac acccgccgcg agatcgaggt cggcggtgtc cgcatcgcgg ctgccggggt ggatgatccg catctgcacc gggaccgata cgacaccatc gccggcatgc cgaaccccct ggcgacattg cgactgggcc tgactcactc ccccgaaccg cgggtgctgg atcgcttcgc cgaggacggc tatcagctgg tgatggccgg acacacccac ggcggtcagc tgtgcctgcc gctctacggc gcgatcgtca ccaactgcga gctggaccgc tctcgggtga aaggcccctc gcagtggggt tcacacatgc agctgcacgt gtcggcgggc atcggaacgt cgccgttcgc gcccatgcga ttcttctgcc gcccggaggc gacactgctg accctggttc cggctcgcac cagcggccac ggggccgacg ccgaatccgg cgatcgctcg cccagcgcca ccgtgcagta aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a