MytuD.01585.a

NADH dehydrogenase subunit E

CENTER ID: MytuD.01585.a
ORGANISM: Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE: Tuberculosis
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MytuD.01585.a.A1.GE26572 Full length( MytuD.01585.a ) 1 252
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|83332.12.peg.3515
RefSeq: NP_217665.1
TubercuList: Rv3149
MTB Network Portal: Rv3149
UniProt: P9WIV5

Orthologues for Rv3149

Species % Identity % Coverage Clones Proteins Structures
ulcerans 87 99 1 0 0
marinum 86 99 1 0 0
avium 84 98 1 0 0
paratuberculosis 84 97 1 0 0
smegmatis 66 95 1 0 0
abscessus 63 91 1 0 0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTQPPGQPVF IRLGPPPDEP NQFVVEGAPR SYPPDVLARL EVDAKEIIGR YPDRRSALLP LLHLVQGEDS YLTPAGLRFC ADQLGLTGAE VSAVASFYTM YRRRPTGEYL VGVCTNTLCA VMGGDAIFDR LKEHLGVGHD ETTSDGVVTL QHIECNAACD YAPVVMVNWE FFDNQTPESA RELVDSLRSD TPKAPTRGAP LCGFRQTSRI LAGLPDQRPD EGQGGPGAPT LAGLQVARKN DMQAPPTPGA DE
NT Sequence
GTGACACAGC CACCCGGTCA GCCGGTGTTC ATCCGGCTCG GACCGCCACC GGACGAACCC AACCAGTTTG TCGTCGAGGG CGCTCCGCGG TCGTATCCGC CGGACGTACT GGCGCGGCTG GAGGTCGACG CCAAGGAGAT CATCGGCCGC TATCCCGACA GGCGCTCGGC GCTGTTGCCG TTGCTGCACC TGGTGCAGGG CGAGGATTCC TACCTGACGC CGGCGGGTTT GCGGTTCTGC GCCGATCAAC TCGGGCTGAC CGGGGCCGAG GTGTCGGCGG TGGCCAGCTT CTACACCATG TACCGCCGGC GCCCCACCGG CGAGTACCTG GTGGGTGTGT GCACGAACAC GCTGTGCGCC GTCATGGGCG GCGACGCCAT CTTCGACCGC CTCAAAGAGC ATCTCGGCGT CGGCCACGAC GAAACCACCT CCGACGGTGT GGTCACCTTG CAACACATCG AATGCAACGC CGCCTGCGAT TACGCACCGG TGGTGATGGT CAACTGGGAA TTCTTCGACA ACCAGACGCC GGAGTCCGCG CGCGAACTCG TCGACTCGCT GCGCTCCGAC ACACCGAAGG CGCCCACCCG CGGCGCGCCG CTGTGCGGCT TCCGGCAAAC ATCGCGCATC CTGGCGGGTC TACCCGACCA GCGTCCCGAC GAAGGCCAGG GCGGTCCCGG CGCGCCCACC CTGGCCGGGC TGCAGGTGGC AAGGAAGAAC GACATGCAGG CGCCACCAAC CCCCGGAGCG GACGAA
Details for MytuD.01585.a.A1.GE26572
HARVESTED ON: 4/23/2009
SEQUENCED ON: 5/21/2009
EXPECTED MW: 29kDa
OBSERVED MW: 30kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVTQPPGQP VFIRLGPPPD EPNQFVVEGA PRSYPPDVLA RLEVDAKEII GRYPDRRSAL LPLLHLVQGE DSYLTPAGLR FCADQLGLTG AEVSAVASFY TMYRRRPTGE YLVGVCTNTL CAVMGGDAIF DRLKEHLGVG HDETTSDGVV TLQHIECNAA CDYAPVVMVN WEFFDNQTPE SARELVDSLR SDTPKAPTRG APLCGFRQTS RILAGLPDQR PDEGQGGPGA PTLAGLQVAR KNDMQAPPTP GA
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggtga cacagccacc cggtcagccg gtgttcatcc ggctcggacc gccaccggac gaacccaacc agtttgtcgt cgagggcgct ccgcggtcgt atccgccgga cgtactggcg cggctggagg tcgacgccaa ggagatcatc ggccgctatc ccgacaggcg ctcggcgctg ttgccgttgc tgcacctggt gcagggcgag gattcctacc tgacgccggc gggtttgcgg ttctgcgccg atcaactcgg gctgaccggg gccgaggtgt cggcggtggc cagcttctac accatgtacc gccggcgccc caccggcgag tacctggtgg gtgtgtgcac gaacacgctg tgcgccgtca tgggcggcga cgccatcttc gaccgcctca aagagcatct cggcgtcggc cacgacgaaa ccacctccga cggtgtggtc accttgcaac acatcgaatg caacgccgcc tgcgattacg caccggtggt gatggtcaac tgggaattct tcgacaacca gacgccggag tccgcgcgcg aactcgtcga ctcgctgcgc tccgacacac cgaaggcgcc cacccgcggc gcgccgctgt gcggcttccg gcaaacatcg cgcatcctgg cgggtctacc cgaccagcgt cccgacgaag gccagggcgg tcccggcgcg cccaccctgg ccgggctgca ggtggcaagg aagaacgaca tgcaggcgcc accaaccccc ggagcgg
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVTQPPGQP VFIRLGPPPD EPNQFVVEGA PRSYPPDVLA RLEVDAKEII GRYPDRRSAL LPLLHLVQGE DSYLTPAGLR FCADQLGLTG AEVSAVASFY TMYRRRPTGE YLVGVCTNTL CAVMGGDAIF DRLKEHLGVG HDETTSDGVV TLQHIECNAA CDYAPVVMVN WEFFDNQTPE SARELVDSLR SDTPKAPTRG APLCGFRQTS RILAGLPDQR PDEGQGGPGA PTLAGLQVAR KNDMQAPPTP GADE
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgacacag ccacccggtc agccggtgtt catccggctc ggaccgccac cggacgaacc caaccagttt gtcgtcgagg gcgctccgcg gtcgtatccg ccggacgtac tggcgcggct ggaggtcgac gccaaggaga tcatcggccg ctatcccgac aggcgctcgg cgctgttgcc gttgctgcac ctggtgcagg gcgaggattc ctacctgacg ccggcgggtt tgcggttctg cgccgatcaa ctcgggctga ccggggccga ggtgtcggcg gtggccagct tctacaccat gtaccgccgg cgccccaccg gcgagtacct ggtgggtgtg tgcacgaaca cgctgtgcgc cgtcatgggc ggcgacgcca tcttcgaccg cctcaaagag catctcggcg tcggccacga cgaaaccacc tccgacggtg tggtcacctt gcaacacatc gaatgcaacg ccgcctgcga ttacgcaccg gtggtgatgg tcaactggga attcttcgac aaccagacgc cggagtccgc gcgcgaactc gtcgactcgc tgcgctccga cacaccgaag gcgcccaccc gcggcgcgcc gctgtgcggc ttccggcaaa catcgcgcat cctggcgggt ctacccgacc agcgtcccga cgaaggccag ggcggtcccg gcgcgcccac cctggccggg ctgcaggtgg caaggaagaa cgacatgcag gcgccaccaa cccccggagc ggacgaaaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa