SSGCID: Target Page

MyulA.01585.a

NADH dehydrogenase I (Chain E) NuoE (NADH-ubiquinone oxidoreductase chain E)

CENTER ID:	MyulA.01585.a
ORGANISM:	Mycobacterium ulcerans Agy99
ASSOCIATED DISEASE:	Buruli, Bairnsdale ulcer
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyulA.01585.a.A1.GE29908	Full length( MyulA.01585.a )		1	251

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|362242.7.peg.2896
RefSeq:	YP_906282.1
UniProt:	A0PR42

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MNAPQSQPVF VRLGPPPEEP NQFTVEGAPQ AYPPDALARL EIEAKEIIGR YPDQRSALLP LLHLVQGEDS YLTPAGLQFC ANQLGLTGAE VSAVASFYTM YRRGPTGEYL VGVCTNTLCA VMGGDAIFET LKDHLGVGND ETTSDGSVTL GHIECNAACD YAPVVMVNWE FFDNQTPESA RELVDSLRSG EPIAPTRGAP LCGFRQTSRI LAGLPDTRPD QGQGGAGAAT LAGLRVAQDN DMQAPPAPGG Q

NT Sequence

GTGAACGCGC CACAGAGTCA GCCGGTATTC GTCCGGCTCG GACCGCCACC CGAGGAGCCC AACCAATTCA CGGTTGAGGG CGCCCCGCAG GCCTACCCGC CGGATGCGCT GGCACGGCTG GAGATCGAAG CCAAGGAGAT CATCGGCCGC TATCCCGACC AGCGCTCCGC GCTGCTGCCG TTGCTGCACC TGGTGCAGGG CGAGGACTCC TACCTGACCC CCGCGGGCCT GCAGTTCTGC GCAAATCAGT TGGGCCTGAC CGGAGCCGAG GTTTCGGCGG TGGCCAGCTT CTACACCATG TATCGCCGTG GGCCGACGGG CGAATACCTG GTCGGCGTCT GCACCAACAC CCTGTGCGCG GTGATGGGCG GCGACGCAAT CTTTGAGACC CTCAAAGACC ACCTCGGCGT CGGCAACGAC GAGACCACCT CCGATGGCTC GGTCACCTTG GGGCACATCG AATGCAACGC GGCCTGCGAC TACGCGCCGG TGGTGATGGT CAACTGGGAA TTCTTTGACA ACCAGACACC GGAATCGGCG CGCGAACTCG TTGACTCGCT GCGCTCGGGC GAACCGATCG CGCCTACCCG GGGCGCTCCG CTGTGCGGCT TCCGCCAGAC ATCGCGCATT CTGGCCGGCT TGCCCGACAC CCGGCCCGAC CAGGGCCAGG GCGGTGCGGG TGCCGCGACA TTAGCCGGAC TGCGGGTGGC GCAAGACAAC GACATGCAGG CACCGCCGGC ACCTGGAGGG CAG

Details for MyulA.01585.a.A1.GE29908

HARVESTED ON:	7/20/2010
SEQUENCED ON:	8/2/2010
EXPECTED MW:	28kDa
OBSERVED MW:	29kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with sequence variation
PERCENT IDENTITY:	97
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG STVNAPQSQP VFVRLGPPPE EPNQFTVEGA PQAYPPDALA RLEIEAKEII GRYPDQRSAL LPLLHLVQGE DSYLTPAGLQ FCANQLGLTG AEVSAVASFY TMYRRGPTGE YLVGVCTNTL CAVMGGDAIF ETLKDHLGVG NDETTSDGSV TLGHIECNAA CDYAPVVMVN WEFFDNQTPE SARELVXSLR SGEXIAPTRG APLCGFRQTS RILAGLXDTR PDXGQGGAGA ATLAGLRVAQ DNDMQAPPAP GX

Validated NT Sequence

ttttgtttac tttaagaagg agatatacca tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgacggtgaa cgcgccacag agtcagccgg tattcgtccg gctcggaccg ccacccgagg agcccaacca attcacggtt gagggcgccc cgcaggccta cccgccggat gcgctggcac ggctggagat cgaagccaag gagatcatcg gccgctatcc cgaccagcgc tccgcgctgc tgccgttgct gcacctggtg cagggcgagg actcctacct gacccccgcg ggcctgcagt tctgcgcaaa tcagttgggc ctgaccggag ccgaggtttc ggcggtggcc agcttctaca ccatgtatcg ccgtgggccg acgggcgaat acctggtcgg cgtctgcacc aacaccctgt gcgcggtgat gggcggcgac gcaatctttg agaccctcaa agaccacctc ggcgtcggca acgacgagac cacctccgat ggctcggtca ccttggggca catcgaatgc aacgcggcct gcgactacgc gccggtggtg atggtcaact gggaattctt tgacaaccag acaccggaat cggcgcgcga actcgttgan tcgctgcgct cgggcgaanc gatcgcgcct acccggggcg ctccgctgtg cggcttccgc cagacatcgc gcattctggc cggcttgncc gacacccggc ccgaccangg ccagggcggt gcgggtgccg cgacattagc cggactgcgg gtggcgcaag acaacgacat gcaggcaccg ccggcacctg gnnggtaata aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct ganttggctg ctgccnnnnn nnnnaanna

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMVNAPQSQP VFVRLGPPPE EPNQFTVEGA PQAYPPDALA RLEIEAKEII GRYPDQRSAL LPLLHLVQGE DSYLTPAGLQ FCANQLGLTG AEVSAVASFY TMYRRGPTGE YLVGVCTNTL CAVMGGDAIF ETLKDHLGVG NDETTSDGSV TLGHIECNAA CDYAPVVMVN WEFFDNQTPE SARELVDSLR SGEPIAPTRG APLCGFRQTS RILAGLPDTR PDQGQGGAGA ATLAGLRVAQ DNDMQAPPAP GG

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgaacgcg ccacagagtc agccggtatt cgtccggctc ggaccgccac ccgaggagcc caaccaattc acggttgagg gcgccccgca ggcctacccg ccggatgcgc tggcacggct ggagatcgaa gccaaggaga tcatcggccg ctatcccgac cagcgctccg cgctgctgcc gttgctgcac ctggtgcagg gcgaggactc ctacctgacc cccgcgggcc tgcagttctg cgcaaatcag ttgggcctga ccggagccga ggtttcggcg gtggccagct tctacaccat gtatcgccgt gggccgacgg gcgaatacct ggtcggcgtc tgcaccaaca ccctgtgcgc ggtgatgggc ggcgacgcaa tctttgagac cctcaaagac cacctcggcg tcggcaacga cgagaccacc tccgatggct cggtcacctt ggggcacatc gaatgcaacg cggcctgcga ctacgcgccg gtggtgatgg tcaactggga attctttgac aaccagacac cggaatcggc gcgcgaactc gttgactcgc tgcgctcggg cgaaccgatc gcgcctaccc ggggcgctcc gctgtgcggc ttccgccaga catcgcgcat tctggccggc ttgcccgaca cccggcccga ccagggccag ggcggtgcgg gtgccgcgac attagccgga ctgcgggtgg cgcaagacaa cgacatgcag gcaccgccgg cacctggagg gaaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa