MytuD.01570.a

2-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinase

CENTER ID: MytuD.01570.a
ORGANISM: Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE: Tuberculosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MytuD.01570.a.B1.GE40366 Full length(MytuD.01570.a) 1 188
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
External Resources
RESOURCE REFERENCE ID
PATRIC ID: fig|83332.12.peg.4023
TubercuList: Rv3606c
MTB Network Portal: Rv3606c
UniProt: P9WNC7
Orthologues for Rv3606c
Species % Identity % Coverage Clones Proteins Structures
avium 74 89 1 1 0
paratuberculosis 74 89 1 1 0
marinum 67 90 0 0 0
ulcerans 66 90 0 0 0
leprae 65 96 1 4 0
smegmatis 65 89 1 2 0
fortuitum 63 89 1 2 0
thermoresistibile 60 87 1 0 0
abscessus 53 88 1 1 0
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTRVVLSVGS NLGDRLARLR SVADGLGDAL IAASPIYEAD PWGGVEQGQF LNAVLIADDP TCEPREWLRR AQEFERAAGR VRGQRWGPRN LDVDLIACYQ TSATEALVEV TARENHLTLP HPLAHLRAFV LIPWIAVDPT AQLTVAGCPR PVTRLLAELE PADRDSVRLF RPSFDLNSRH PVSRAPES
NT Sequence
ATGACGCGGG TAGTGCTCTC GGTTGGCTCC AACCTGGGTG ACCGCCTGGC ACGATTGCGG TCGGTCGCCG ACGGTCTCGG CGATGCGTTG ATTGCGGCTT CCCCGATATA TGAGGCCGAC CCCTGGGGTG GGGTGGAGCA GGGGCAGTTC CTCAATGCGG TGCTGATCGC CGACGATCCT ACCTGCGAAC CGCGGGAGTG GCTGCGGCGG GCGCAGGAGT TCGAGCGCGC TGCGGGCAGG GTGCGTGGCC AGCGCTGGGG TCCACGAAAT CTCGACGTCG ACCTGATCGC CTGCTACCAG ACCTCGGCCA CCGAGGCTCT GGTCGAAGTG ACCGCGCGGG AGAACCACCT CACGCTGCCG CACCCACTGG CGCATCTGCG GGCCTTTGTG TTGATCCCGT GGATTGCCGT CGACCCAACG GCGCAGCTGA CGGTTGCCGG GTGCCCGCGG CCCGTCACGC GACTGCTGGC CGAGCTGGAG CCCGCCGACC GCGACAGTGT GCGGTTGTTT AGGCCGTCGT TCGATCTGAA TAGCAGACAC CCCGTCAGTC GGGCACCGGA AAGC
Details for MytuD.01570.a.B1.GE40366
HARVESTED ON: 7/15/2016
SEQUENCED ON: 7/18/2016
EXPECTED MW: 22kDa
OBSERVED MW: 22kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMT RVVLSVGSNL GDRLARLRSV ADGLGDALIA ASPIYEADPW GGVEQGQFLN AVLIADDPTC EPREWLRRAQ EFERAAGRVR GQRWGPRNLD VDLIACYQTS ATEALVEVTA RENHLTLPHP LAHLRAFVLI PWIAVDPTAQ LTVAGCPRPV TRLLAELEPA DRDSVRLFRP SFDLNSRHPV SRAPES
Validated NT Sequence
atcttactca cttagctttc cggtgcccga ctgacggggt gtctgctatt cagatcgaac gacggcctaa acaaccgcac actgtcgcgg tcggcgggct ccagctcggc cagcagtcgc gtgacgggcc gcgggcaccc ggcaaccgtc agctgcgccg ttgggtcgac ggcaatccac gggatcaaca caaaggcccg cagatgcgcc agtgggtgcg gcagcgtgag gtggttctcc cgcgcggtca cttcgaccag agcctcggtg gccgaggtct ggtagcaggc gatcaggtcg acgtcgagat ttcgtggacc ccagcgctgg ccacgcaccc tgcccgcagc gcgctcgaac tcctgcgccc gccgcagcca ctcccgcggt tcgcaggtag gatcgtcggc gatcagcacc gcattgagga actgcccctg ctccacccca ccccaggggt cggcctcata tatcggggaa gccgcaatca acgcatcgcc gagaccgtcg gcgaccgacc gcaatcgtgc caggcggtca cccaggttgg agccaaccga gagcactacc cgcgtcatat ggtggtggtg gtggtgagcc at
Expected Protein Sequence
MAHHHHHHMT RVVLSVGSNL GDRLARLRSV ADGLGDALIA ASPIYEADPW GGVEQGQFLN AVLIADDPTC EPREWLRRAQ EFERAAGRVR GQRWGPRNLD VDLIACYQTS ATEALVEVTA RENHLTLPHP LAHLRAFVLI PWIAVDPTAQ LTVAGCPRPV TRLLAELEPA DRDSVRLFRP SFDLNSRHPV SRAPES
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgacgc gggtagtgct ctcggttggc tccaacctgg gtgaccgcct ggcacgattg cggtcggtcg ccgacggtct cggcgatgcg ttgattgcgg cttccccgat atatgaggcc gacccctggg gtggggtgga gcaggggcag ttcctcaatg cggtgctgat cgccgacgat cctacctgcg aaccgcggga gtggctgcgg cgggcgcagg agttcgagcg cgctgcgggc agggtgcgtg gccagcgctg gggtccacga aatctcgacg tcgacctgat cgcctgctac cagacctcgg ccaccgaggc tctggtcgaa gtgaccgcgc gggagaacca cctcacgctg ccgcacccac tggcgcatct gcgggccttt gtgttgatcc cgtggattgc cgtcgaccca acggcgcagc tgacggttgc cgggtgcccg cggcccgtca cgcgactgct ggccgagctg gagcccgccg accgcgacag tgtgcggttg tttaggccgt cgttcgatct gaatagcaga caccccgtca gtcgggcacc ggaaagctga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at