MythA.01570.a

2-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinase (Rv3606c ortholog)

CENTER ID:	MythA.01570.a
ORGANISM:	Mycobacterium thermoresistibile ATCC 19527 / NCTC 10409
ASSOCIATED DISEASE:	Respiratory Infection
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MythA.01570.a.B1.GE37647	full length		1	174

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
PATRIC ID:	fig\|1078020.3.peg.1563
UniProt:	G7CF19

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MVLSIGSNLG DRLAHLQSVV DALGAAVSAV SPVYETEAWG GVEQGRFLNA TLIAETPALD CHGWLDTAQQ LERAADRVRE RRWGPRTLDV DLITCHDGDR EVRMDDERLI LPHPYAHQRA FVLVPWLAID PGAVLTVAGT PRPVAGLLAG LDPAERAGVH RTDLTLRIDP RRGA

NT Sequence

gtggtgctgt cgatcggttc gaacctcggg gaccggctgg cgcatctgca gtcggtcgtc gacgcgctcg gcgccgcggt gtcggcggtc tcaccggtgt acgagaccga ggcctggggt ggtgtcgagc agggccgctt cctcaatgcg acgctgatcg ccgagacgcc cgcactggac tgccacggct ggctggacac cgcccagcag ctggagcgcg ccgccgaccg cgtccgcgaa cggcgttggg ggccacgcac cctcgacgtc gacctgatca cctgccacga cggtgaccgg gaagtgcgga tggacgacga gcggttgatc ctgccgcacc cctacgcgca tcagcgggcg ttcgtactgg tgccgtggct ggcgatcgac ccgggcgccg tgctgacggt ggccggcacc ccgcggccgg tcgccggtct gctggccgga ctggaccccg ccgagcgggc cggggtgcac cgcacggacc tgacgctgcg gatcgatccg cggcgcgggg cgtga

Details for MythA.01570.a.B1.GE37647

HARVESTED ON:	1/13/2014
SEQUENCED ON:	1/9/2014
EXPECTED MW:	20kDa
OBSERVED MW:	20kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMV VLSIGSNLGD RLAHLQSVVD ALGAAVSAVS PVYETEAWGG VEQGRFLNAT LIAETPALDC HGWLDTAQQL ERAADRVRER RWGPRTLDVD LITCHDGDRE VRMDDERLIL PHPYAHQRAF VLVPWLAIDP GAVLTVAGTP RPVAGLLAGL DPAERAGVHR TDLTLRIDPR RGA

Validated NT Sequence

ggggggntgn tagcagccgg atcctatctt actcacttac gccccgcgcc gcggatcgat ccgcagcgtc aggtccgtgc ggtgcacccc ggcccgctcg gcggggtcca gtccggccag cagaccggcg accggccgcg gggtgccggc caccgtcagc acggcgcccg ggtcgatcgc cagccacggc accagtacga acgcccgctg atgcgcgtag gggtgcggca ggatcaaccg ctcgtcgtcc atccgcactt cccggtcacc gtcgtggcag gtgatcaggt cgacgtcgag ggtgcgtggc ccccaacgcc gttcgcggac gcggtcggcg gcgcgctcca gctgctgggc ggtgtccagc cagccgtggc agtccagtgc gggcgtctcg gcgatcagcg tcgcattgag gaagcggccc tgctcgacac caccccaggc ctcggtctcg tacaccggtg agaccgccga caccgcggcg ccgagcgcgt cgacgaccga ctgcagatgc gccagccggt ccccgaggtt cgaaccgatc gacagcacca ccatatggtg gtggtggtgg tgagccat

Expected Protein Sequence

MAHHHHHHMV LSIGSNLGDR LAHLQSVVDA LGAAVSAVSP VYETEAWGGV EQGRFLNATL IAETPALDCH GWLDTAQQLE RAADRVRERR WGPRTLDVDL ITCHDGDREV RMDDERLILP HPYAHQRAFV LVPWLAIDPG AVLTVAGTPR PVAGLLAGLD PAERAGVHRT DLTLRIDPRR GA

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtggtgc tgtcgatcgg ttcgaacctc ggggaccggc tggcgcatct gcagtcggtc gtcgacgcgc tcggcgccgc ggtgtcggcg gtctcaccgg tgtacgagac cgaggcctgg ggtggtgtcg agcagggccg cttcctcaat gcgacgctga tcgccgagac gcccgcactg gactgccacg gctggctgga caccgcccag cagctggagc gcgccgccga ccgcgtccgc gaacggcgtt gggggccacg caccctcgac gtcgacctga tcacctgcca cgacggtgac cgggaagtgc ggatggacga cgagcggttg atcctgccgc acccctacgc gcatcagcgg gcgttcgtac tggtgccgtg gctggcgatc gacccgggcg ccgtgctgac ggtggccggc accccgcggc cggtcgccgg tctgctggcc ggactggacc ccgccgagcg ggccggggtg caccgcacgg acctgacgct gcggatcgat ccgcggcgcg gggcgtgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat