MytuD.01356.a

PROBABLE UDP-GLUCOSE 6-DEHYDROGENASE UDGA (UDP-GLC DEHYDROGENASE) (UDP-GLCDH) (UDPGDH)

CENTER ID: MytuD.01356.a
ORGANISM: Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE: Tuberculosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MytuD.01356.a.B1.GE27180 Full length( MytuD.01356.a ) 1 443
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|83332.12.peg.356
RefSeq: NP_214836.1
TubercuList: Rv0322
MTB Network Portal: Rv0322
UniProt: O07248

Orthologues for Rv0322

Species % Identity % Coverage Clones Proteins Structures
marinum 90 99 1 0 0
smegmatis 85 98 0 0 0
abscessus 80 100 1 0 0
smegmatis 68 99 0 0 0
avium 39 98 1 0 0
paratuberculosis 38 98 0 0 0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRCSVFGTGY LGATHAVGMA QLGHEVVGVD IDPGKVAKLA GGDIPFYEPG LRKLLTDNLA AGRLRFTTDY DMAADFADVH FLGVGTPQKI GEYGADLRHV HAVIDALVPR LVRASILVGK STVPVGTAAE LGHRAGALAP RGVDVEIAWN PEFLREGFAV HDTLNPDRIV LGVQDDSTRA EVAVRELYAP LLAAGVPFLV TDLQTAELVK VSANAFLATK ISFINAISEV CEAAGADVSQ LADALGYDPR IGRQCLNAGL GFGGGCLPKD IRAFMARAGE LGADQALTFL REVDSINMRR RTKMVELATT ACGGSLLGAN IAVLGAAFKP ESDDVRDSPA LNVAGQLQLN GATVHVYDPK ALDNAHRLFP TLNYAVSVAE ACERADAVLV LTEWREFIDL EPADLANRVR ARVIVDGRNC LDVTRWRRAG WRVFRLGVPR LGH
NT Sequence
GTGCGATGCA GCGTCTTCGG CACTGGGTAT CTGGGTGCCA CCCACGCCGT CGGTATGGCG CAACTGGGAC ACGAGGTCGT CGGGGTCGAT ATCGATCCCG GTAAGGTCGC CAAGCTCGCC GGGGGTGACA TTCCGTTCTA CGAACCCGGC CTGCGAAAGC TGTTGACTGA TAACCTGGCT GCCGGCCGCT TGCGGTTCAC CACCGACTAC GACATGGCGG CCGATTTCGC CGACGTGCAT TTCCTGGGGG TCGGCACGCC GCAAAAGATA GGCGAATATG GCGCCGACCT GCGGCATGTC CACGCCGTCA TCGATGCGCT GGTGCCGCGT CTGGTCAGGG CGTCGATTCT GGTCGGCAAG TCGACAGTCC CAGTGGGCAC CGCAGCCGAA CTGGGACATC GGGCCGGTGC ACTGGCACCC CGGGGAGTCG ACGTGGAAAT TGCCTGGAAT CCGGAATTCC TGCGCGAGGG CTTCGCGGTG CACGACACCC TCAACCCCGA CCGTATCGTC CTTGGGGTAC AAGATGATTC GACGCGCGCC GAGGTAGCCG TCCGCGAGCT GTACGCGCCG CTGCTGGCAG CGGGCGTGCC GTTTCTGGTG ACCGATCTGC AGACCGCGGA GTTGGTCAAG GTATCCGCCA ATGCCTTTCT GGCGACCAAG ATTTCGTTTA TCAATGCGAT CTCCGAAGTG TGCGAGGCGG CGGGTGCCGA CGTTAGCCAG CTGGCCGATG CGCTCGGATA CGACCCGCGG ATCGGACGCC AATGCCTCAA CGCGGGCTTG GGTTTCGGCG GCGGCTGCTT GCCCAAGGAC ATCCGCGCTT TCATGGCCCG CGCCGGCGAA CTGGGAGCCG ACCAGGCGTT GACGTTCCTG CGTGAAGTGG ACAGCATCAA CATGCGCCGG CGCACCAAGA TGGTGGAACT GGCCACCACC GCATGCGGTG GCTCGTTGCT GGGCGCCAAT ATTGCGGTGC TCGGCGCGGC GTTCAAACCC GAATCCGATG ACGTGCGCGA TTCGCCCGCC CTCAATGTGG CGGGCCAGCT GCAGCTCAAC GGCGCCACGG TCCACGTGTA CGATCCAAAG GCCTTGGACA ACGCCCACCG ACTGTTCCCT ACCTTGAACT ATGCGGTTTC GGTTGCGGAG GCCTGCGAGC GCGCGGACGC CGTGTTGGTG CTTACCGAAT GGCGGGAGTT CATCGATCTC GAACCCGCTG ATCTAGCCAA CCGGGTGCGG GCCCGGGTGA TCGTGGACGG CCGCAACTGC CTCGACGTGA CCCGCTGGCG GCGGGCAGGC TGGCGGGTGT TCCGGCTGGG AGTGCCGCGA TTAGGGCAC
Details for MytuD.01356.a.B1.GE27180
HARVESTED ON: 8/17/2009
SEQUENCED ON: 9/29/2009
EXPECTED MW: 48kDa
OBSERVED MW: 48kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with sequence variation
PERCENT IDENTITY: 99
PERCENT COVERAGE: 92
Validated AA Sequence
MAQLGHEVVG VDIDPGKVAK LAGGDIPFYE PGLRKLLTDN LAAGRLRFTT DYDMAADFAD VHFLGVGTPQ KIGEYGADLR HVHAVIDALV PRLVRASILV GKSTVPVGTA AELGHRAGAL APRGVDVEIA WNPEFLREGF AVHDTLNPDR IVLGVQDDST RAEVAVRELY APLLAAGVPF LVTDLQTAEL VKVSANAFLA TKISFINAIS EVCEAAGADV SQLADALGYD PRIGRQCLNA GLGFGGGCLP KDIRAFMARA GEPGADQALT FLREVDSINM RRRTKMVELA TTACGGSLLG ANIAVLGAAF KPESDDVRDS PALNVAGQLQ LNGATVHVYD PKALDNAHRL FPTLNYAVSV AEACERADAV LVLTEWREFI DLEPADLANR VRARVIVDGR NCLDVTRWRR AGWRVFR
Validated NT Sequence
gccggaacac ccgccagcct gcccgccgcc agcgggtcac gtcgaggcag ttgcggccgt ccacgatcac ccgggcccgc acccggttgg ctagatcagc gggttcgaga tcgatgaact cccgccattc ggtaagcacc aacacggcgt ccgcgcgctc gcaggcctcc gcaaccgaaa ccgcatagtt caaggtaggg aacagtcggt gggcgttgtc caaggccttt ggatcgtaca cgtggaccgt ggcgccgttg agctgcagct ggcccgccac attgagggcg ggcgaatcgc gcacgtcatc ggattcgggt ttgaacgccg cgccgagcac cgcaatattg gcgcccagca acgagccacc gcatgcggtg gtggccagtt ccaccatctt ggtgcgccgg cgcatgttga tgctgtccac ttcacgcagg aacgtcaacg cctggtcggc tcccggttcg ccggcgcggg ccatgaaagc gcggatgtcc ttgggcaagc agccgccgcc gaaacccaag cccgcgttga ggcattggcg tccgatccgc gggtcgtatc cgagcgcatc ggccagctgg ctaacgtcgg cacccgccgc ctcgcacact tcggagatcg cattgataaa cgaaatcttg gtcgccagaa aggcattggc ggataccttg accaactccg cggtctgcag atcggtcacc agaaacggca cgcccgctgc cagcagcggc gcgtacagct cgcggacggc tacctcggcg cgcgtcgaat catcttgtac cccaaggacg atacggtcgg ggttgagggt gtcgtgcacc gcgaagccct cgcgcaggaa ttccggattc caggcaattt ccacgtcgac tccccggggt gccagtgcac cggcccgatg tcccagttcg gctgcggtgc ccactgggac tgtcgacttg ccgaccagaa tcgacgccct gaccagacgc ggcaccagcg catcgatgac ggcgtggaca tgccgcaggt cggcgccata ttcgcctatc ttttgcggcg tgccgacccc caggaaatgc acgtcggcga aatcggccgc catgtcgtag tcggtggtga accgcaagcg gccggcagcc aggttatcag tcaacagctt tcgcaggccg ggttcgtaga acggaatgtc acccccggcg agcttggcga ccttaccggg atcgatatcg accccgacga cctcgtgtcc cagttgcgcc ataccgacgg cgtgggtggc acccagatac ccagtgccga agacgctgca tc
Expected Protein Sequence
MAHHHHHHMV RCSVFGTGYL GATHAVGMAQ LGHEVVGVDI DPGKVAKLAG GDIPFYEPGL RKLLTDNLAA GRLRFTTDYD MAADFADVHF LGVGTPQKIG EYGADLRHVH AVIDALVPRL VRASILVGKS TVPVGTAAEL GHRAGALAPR GVDVEIAWNP EFLREGFAVH DTLNPDRIVL GVQDDSTRAE VAVRELYAPL LAAGVPFLVT DLQTAELVKV SANAFLATKI SFINAISEVC EAAGADVSQL ADALGYDPRI GRQCLNAGLG FGGGCLPKDI RAFMARAGEL GADQALTFLR EVDSINMRRR TKMVELATTA CGGSLLGANI AVLGAAFKPE SDDVRDSPAL NVAGQLQLNG ATVHVYDPKA LDNAHRLFPT LNYAVSVAEA CERADAVLVL TEWREFIDLE PADLANRVRA RVIVDGRNCL DVTRWRRAGW RVFRLGVPRL GH
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggtgc gatgcagcgt cttcggcact gggtatctgg gtgccaccca cgccgtcggt atggcgcaac tgggacacga ggtcgtcggg gtcgatatcg atcccggtaa ggtcgccaag ctcgccgggg gtgacattcc gttctacgaa cccggcctgc gaaagctgtt gactgataac ctggctgccg gccgcttgcg gttcaccacc gactacgaca tggcggccga tttcgccgac gtgcatttcc tgggggtcgg cacgccgcaa aagataggcg aatatggcgc cgacctgcgg catgtccacg ccgtcatcga tgcgctggtg ccgcgtctgg tcagggcgtc gattctggtc ggcaagtcga cagtcccagt gggcaccgca gccgaactgg gacatcgggc cggtgcactg gcaccccggg gagtcgacgt ggaaattgcc tggaatccgg aattcctgcg cgagggcttc gcggtgcacg acaccctcaa ccccgaccgt atcgtccttg gggtacaaga tgattcgacg cgcgccgagg tagccgtccg cgagctgtac gcgccgctgc tggcagcggg cgtgccgttt ctggtgaccg atctgcagac cgcggagttg gtcaaggtat ccgccaatgc ctttctggcg accaagattt cgtttatcaa tgcgatctcc gaagtgtgcg aggcggcggg tgccgacgtt agccagctgg ccgatgcgct cggatacgac ccgcggatcg gacgccaatg cctcaacgcg ggcttgggtt tcggcggcgg ctgcttgccc aaggacatcc gcgctttcat ggcccgcgcc ggcgaactgg gagccgacca ggcgttgacg ttcctgcgtg aagtggacag catcaacatg cgccggcgca ccaagatggt ggaactggcc accaccgcat gcggtggctc gttgctgggc gccaatattg cggtgctcgg cgcggcgttc aaacccgaat ccgatgacgt gcgcgattcg cccgccctca atgtggcggg ccagctgcag ctcaacggcg ccacggtcca cgtgtacgat ccaaaggcct tggacaacgc ccaccgactg ttccctacct tgaactatgc ggtttcggtt gcggaggcct gcgagcgcgc ggacgccgtg ttggtgctta ccgaatggcg ggagttcatc gatctcgaac ccgctgatct agccaaccgg gtgcgggccc gggtgatcgt ggacggccgc aactgcctcg acgtgacccg ctggcggcgg gcaggctggc gggtgttccg gctgggagtg ccgcgattag ggcactgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat