MyabA.01356.a

Probable UDP-glucose 6-dehydrogenase (UdgA)

CENTER ID: MyabA.01356.a
ORGANISM: Mycobacterium abscessus ATCC 19977 / DSM 44196
ASSOCIATED DISEASE: Skin abscess
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyabA.01356.a.A2.GE30503 Mature protein( MyabA.01356.a ) 16 446
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|36809.5.peg.4375
RefSeq: YP_001705022.1
UniProt: B1MIZ7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRCTVFGTGY LGATHAACMA ELGHEVLGVD IDPGKVAKLS SGDIPFYEPG LRKILQSNIA SGRLRFTTSY AEAAEFADVH FLGVGTPQKK GEYGADLRHV HSVIDTLVPL LSRSAVIIGK STVPVGTARE LGQRAAGFAA AGVDVEVAWN PEFLREGYAV KDTLHPDRIV LGVQRDSDRA EKLVRELYGP ILDEEVPFLV TDLETAELVK VSANAFLATK ISFINAISEV CEAVGADVTT LADALGYDPR IGRRFLNAGL GFGGGCLPKD IRAFMARAGE LGASHALTFL REVDSINMRR RTRMVELATK ACGGSLLGAN VAVLGAAFKP ESDDVRDSPA LNVAGLLQLN GAAVNVYDPK ALENSRRLFP TLNYATSVLE AADRADAVLL LTEWQEFVDF DPDALAKVVR TKVIVDGRNC LDAAKWVNAG WRVFCLGKRV EELAHT
NT Sequence
ATGAGATGCA CCGTATTCGG AACCGGGTAC CTCGGAGCCA CCCATGCCGC ATGCATGGCC GAGCTGGGCC ACGAGGTGCT CGGCGTCGAC ATCGATCCGG GTAAGGTCGC CAAACTCTCG TCGGGCGACA TCCCGTTCTA CGAGCCCGGA CTGCGAAAGA TCTTGCAAAG CAACATCGCA TCCGGACGCC TGCGCTTTAC CACAAGCTAT GCCGAAGCCG CCGAGTTCGC CGATGTGCAC TTCCTGGGGG TCGGCACCCC CCAGAAGAAG GGTGAGTACG GCGCGGACCT GCGTCACGTC CACTCGGTGA TCGACACCCT GGTGCCCCTA CTGTCGCGCT CGGCGGTGAT CATCGGCAAG TCCACGGTTC CGGTCGGCAC CGCACGCGAA CTGGGCCAGC GGGCCGCAGG ATTCGCGGCA GCAGGAGTGG ACGTTGAGGT GGCCTGGAAC CCTGAGTTCC TGCGTGAGGG CTACGCCGTC AAGGACACGC TGCACCCCGA CCGCATTGTG CTGGGTGTGC AGCGGGATTC GGACCGCGCC GAGAAACTGG TGCGCGAGTT GTACGGGCCG ATCCTCGATG AAGAAGTTCC GTTCTTGGTC ACCGACCTGG AAACCGCAGA ACTGGTGAAG GTTTCGGCCA ATGCCTTCCT GGCCACCAAG ATCTCGTTCA TCAACGCCAT CTCCGAGGTA TGCGAGGCGG TGGGTGCGGA CGTGACCACG CTGGCCGACG CTCTGGGCTA CGACCCGCGA ATCGGCCGGC GGTTCCTCAA TGCGGGGCTG GGTTTCGGCG GAGGATGCCT GCCCAAGGAC ATCCGGGCGT TCATGGCGCG CGCGGGCGAA CTGGGAGCCA GTCACGCGCT GACCTTCCTG CGCGAGGTGG ACAGCATCAA CATGCGGCGG CGCACCCGCA TGGTGGAGCT GGCGACCAAG GCATGCGGCG GATCGCTGTT GGGCGCCAAT GTCGCGGTGC TGGGCGCGGC GTTCAAGCCC GAGTCCGATG ATGTCCGTGA TTCACCAGCG CTGAACGTGG CCGGACTGTT GCAGCTTAAC GGCGCGGCCG TCAACGTCTA TGACCCGAAG GCACTGGAGA ATTCGCGCCG CCTGTTCCCG ACGCTGAACT ACGCCACCTC GGTTCTGGAA GCCGCCGACC GCGCCGACGC GGTGCTGCTG CTCACCGAGT GGCAGGAGTT CGTCGACTTC GATCCGGATG CGCTGGCCAA GGTGGTGCGC ACCAAAGTTA TTGTGGACGG GCGTAATTGC CTGGATGCCG CCAAGTGGGT CAACGCCGGA TGGCGAGTGT TCTGCCTGGG CAAGCGAGTG GAAGAGCTGG CCCACACC
Details for MyabA.01356.a.A2.GE30503
HARVESTED ON: 9/21/2010
SEQUENCED ON: 9/28/2010
EXPECTED MW: 48kDa
OBSERVED MW: 49kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 91
PERCENT COVERAGE: 74
Validated AA Sequence
MAHHXHHHMG TLEAQTQGPG SMAACXXXXX HEVLGVDIDP GKVAKLSSGD IPFYEPGLRK ILQSNIASGR LRFTTSYAEA AEFADVHFLG VGTPQKKGEY GADLRHVHSV IDTLVPLLSR SAVIIGKSTV PVGTARELGQ RAAGFAAAGV DVEVAWNPEF LREGYAVKDT LHPDRIVLGV QRDSDRAEKL VRELYGPILD EEVPFLVTDL ETAELVKVSA NAFLATKISF INAISEVCEA VGADVTTLAD ALGYDPRIGR RXLNAGLGFG GXCLPKDIRA FMARAGELGA SHALTFLREV XSINMXRRTR XXXXXDHAXE XLXGANVXXL GXXXAXXX
Validated NT Sequence
ttnttnactt taagaaggag atataccatg gctcatcacc ntcaccatca tatgggtacc ctggaagctc agacccaggg tcctggttcg atggccgcat gcntggncga nnnnnnccac gaggtgctcg gcgtcgacat cgatccgggt aaggtcgcca aactctcgtc gggcgacatc ccgttctacg agcccggact gcgaaagatc ttgcaaagca acatcgcatc cggacgcctg cgctttacca caagctatgc cgaagccgcc gagttcgccg atgtgcactt cctgggggtc ggcacccccc agaagaaggg tgagtacggc gcggacctgc gtcacgtcca ctcggtgatc gacaccctgg tgcccctact gtcgcgctcg gcggtgatca tcggcaagtc cacggttccg gtcggcaccg cacgcgaact gggccagcgg gccgcaggat tcgcggcagc aggagtggac gttgaggtgg cctggaaccc tgagttcctg cgtgagggct acgccgtcaa ggacacgctg caccccgacc gcattgtgct gggtgtgcag cgggattcgg accgcgccga gaaactggtg cgcgagttgt acgggccgat cctcgatgaa gaagttccgt tcttggtcac cgacctggaa accgcagaac tggtgaaggt ttcggccaat gccttcctgg ccaccaagat ctcgttcatc aacgccatct ccgaggtatg cgaggcggtg ggtgcggacg tgaccacgct ggccgacgct ctgggctacg acccgcgaat cggccggcgg ttnctcaatg cggggctggg tttcggcgga ngatgcctgc ccaaggacat ccgggcgttc atggcgcgcg cgggcgaact gggagcnagt cacgcgctga ccttcctgcg cgaggtggan agcatcaaca tgcnncggcg cacccgcatn nnnnganctg ncgaccatgc nncggaancg ctnnngggcg ccaatgtcgn nnngctgggn ncngnncnag cccnannncn atgangtccn nganncaccn nnnctgaagg ncnnntnnan ntnancngnn nnnnnnnann ncnnntnann gnnnnnnnnn nnnnccnnnn nnnnnnnnna nnnnnnnnnn annnnncnnn nnngnngnc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAACMAELG HEVLGVDIDP GKVAKLSSGD IPFYEPGLRK ILQSNIASGR LRFTTSYAEA AEFADVHFLG VGTPQKKGEY GADLRHVHSV IDTLVPLLSR SAVIIGKSTV PVGTARELGQ RAAGFAAAGV DVEVAWNPEF LREGYAVKDT LHPDRIVLGV QRDSDRAEKL VRELYGPILD EEVPFLVTDL ETAELVKVSA NAFLATKISF INAISEVCEA VGADVTTLAD ALGYDPRIGR RFLNAGLGFG GGCLPKDIRA FMARAGELGA SHALTFLREV DSINMRRRTR MVELATKACG GSLLGANVAV LGAAFKPESD DVRDSPALNV AGLLQLNGAA VNVYDPKALE NSRRLFPTLN YATSVLEAAD RADAVLLLTE WQEFVDFDPD ALAKVVRTKV IVDGRNCLDA AKWVNAGWRV FCLGKRVEEL AHT
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggccgcatgc atggccgagc tgggccacga ggtgctcggc gtcgacatcg atccgggtaa ggtcgccaaa ctctcgtcgg gcgacatccc gttctacgag cccggactgc gaaagatctt gcaaagcaac atcgcatccg gacgcctgcg ctttaccaca agctatgccg aagccgccga gttcgccgat gtgcacttcc tgggggtcgg caccccccag aagaagggtg agtacggcgc ggacctgcgt cacgtccact cggtgatcga caccctggtg cccctactgt cgcgctcggc ggtgatcatc ggcaagtcca cggttccggt cggcaccgca cgcgaactgg gccagcgggc cgcaggattc gcggcagcag gagtggacgt tgaggtggcc tggaaccctg agttcctgcg tgagggctac gccgtcaagg acacgctgca ccccgaccgc attgtgctgg gtgtgcagcg ggattcggac cgcgccgaga aactggtgcg cgagttgtac gggccgatcc tcgatgaaga agttccgttc ttggtcaccg acctggaaac cgcagaactg gtgaaggttt cggccaatgc cttcctggcc accaagatct cgttcatcaa cgccatctcc gaggtatgcg aggcggtggg tgcggacgtg accacgctgg ccgacgctct gggctacgac ccgcgaatcg gccggcggtt cctcaatgcg gggctgggtt tcggcggagg atgcctgccc aaggacatcc gggcgttcat ggcgcgcgcg ggcgaactgg gagccagtca cgcgctgacc ttcctgcgcg aggtggacag catcaacatg cggcggcgca cccgcatggt ggagctggcg accaaggcat gcggcggatc gctgttgggc gccaatgtcg cggtgctggg cgcggcgttc aagcccgagt ccgatgatgt ccgtgattca ccagcgctga acgtggccgg actgttgcag cttaacggcg cggccgtcaa cgtctatgac ccgaaggcac tggagaattc gcgccgcctg ttcccgacgc tgaactacgc cacctcggtt ctggaagccg ccgaccgcgc cgacgcggtg ctgctgctca ccgagtggca ggagttcgtc gacttcgatc cggatgcgct ggccaaggtg gtgcgcacca aagttattgt ggacgggcgt aattgcctgg atgccgccaa gtgggtcaac gccggatggc gagtgttctg cctgggcaag cgagtggaag agctggccca cacctaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa