MytuD.01347.a

PROBABLE ADHESION COMPONENT TRANSPORT ATP-BINDING PROTEIN ABC TRANSPORTER

CENTER ID: MytuD.01347.a
ORGANISM: Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE: Tuberculosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MytuD.01347.a.B1.GE27178 Full length( MytuD.01347.a ) 1 248
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|83332.12.peg.1100
RefSeq: NP_215501.1
TubercuList: Rv0986
MTB Network Portal: Rv0986
UniProt: P9WQK1

Orthologues for Rv0986

Species % Identity % Coverage Clones Proteins Structures
marinum 45 95 0 0 0
ulcerans 45 95 0 0 0
smegmatis 44 90 0 0 0
abscessus 42 93 0 0 0
thermoresistibile 40 96 1 0 0
fortuitum 40 88 0 0 0
smegmatis 39 87 0 0 0
thermoresistibile 38 82 0 0 0
thermoresistibile 35 95 1 0 0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNRQPIVQLS NLSWTFREGE TRRQVLDHIT FDFEPGEFVA LLGQSGSGKS TLLNLISGIE KPTTGDVTIN GFAITQKTER DRTLFRRDQI GIVFQFFNLI PTLTVLENIT LPQELAGVSQ RKAAVVARDL LEKVGMADRE RTFPDKLSGG EQQRVAISRA LAHNPMLVLA DEPTGNLDSD TGDKVLDVLL DLTRQAGKTL IMATHSPSMT QHADRVVNLQ GGRLIPAVNR ENQTDQPAST ILLPTSYE
NT Sequence
ATGAACCGGC AACCTATCGT TCAGCTGAGT AACTTGAGCT GGACATTCCG AGAAGGCGAA ACCCGACGAC AAGTCCTAGA CCACATCACC TTCGATTTCG AGCCCGGTGA GTTTGTCGCG CTGCTGGGGC AAAGTGGAAG TGGTAAAAGC ACTTTGCTGA ACCTCATCAG TGGCATAGAA AAGCCCACCA CAGGTGACGT CACAATTAAT GGGTTCGCTA TCACTCAGAA AACCGAGCGA GACCGGACGT TGTTCCGGCG CGATCAGATT GGCATCGTCT TTCAATTTTT CAACCTGATT CCCACTCTTA CCGTGTTGGA AAATATTACG CTGCCTCAGG AACTGGCCGG AGTTTCTCAG AGGAAAGCGG CCGTGGTCGC TCGTGACCTT CTCGAAAAAG TGGGCATGGC CGACCGTGAA CGCACCTTTC CCGATAAACT CTCCGGCGGA GAACAACAAC GGGTCGCTAT TTCCAGAGCG TTGGCGCATA ATCCCATGCT GGTGTTAGCC GATGAGCCGA CCGGCAACCT GGACTCCGAT ACCGGGGATA AAGTCTTGGA TGTTCTGCTT GATCTCACCC GCCAAGCAGG TAAAACCTTA ATCATGGCTA CGCATAGCCC GTCGATGACG CAGCATGCCG ACCGGGTAGT CAACTTACAG GGCGGCAGGT TGATACCTGC CGTGAACCGA GAAAATCAAA CCGACCAGCC GGCCAGCACG ATCCTATTGC CCACGTCATA TGAA
Details for MytuD.01347.a.B1.GE27178
HARVESTED ON: 8/17/2009
SEQUENCED ON: 9/29/2009
EXPECTED MW: 28kDa
OBSERVED MW: 28kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with sequence variation
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMN RQPIVQLSNL SWTFREGETR RQVLDHITFD FEPGEFVALL GQSGSGKSTL LNLISGIEKP TTGDVTINGF AITQKTERDR TLFRRDQIGI VFQFFNLIPT LTVLGNITLP QELAGVSQRK AAVVARDLLE KVGMADRERT FPDKLSGGEQ QRVAISRALA HNPMLVLADE PTGNLDSDTG DKVLDVLLDL TRQAGKTLIM ATHSPSMTQH ADRVVNLQGG RLIPAVNREN QTDQPASTIL LPTSYEK
Validated NT Sequence
atcttactca cttttcatat gacgtgggca ataggatcgt gctggccggc tggtcggttt gattttctcg gttcacggca ggtatcaacc tgccgccctg taagttgact acccggtcgg catgctgcgt catcgacggg ctatgcgtag ccatgattaa ggttttacct gcttggcggg tgagatcaag cagaacatcc aagactttat ccccggtatc ggagtccagg ttgccggtcg gctcatcggc taacaccagc atgggattat gcgccaacgc tctggaaata gcgacccgtt gttgttctcc gccggagagt ttatcgggaa aggtgcgttc acggtcggcc atgcccactt tttcgagaag gtcacgagcg accacggccg ctttcctctg agaaactccg gccagttcct gaggcagcgt aatatttccc aacacggtaa gagtgggaat caggttgaaa aattgaaaga cgatgccaat ctgatcgcgc cggaacaacg tccggtctcg ctcggttttc tgagtgatag cgaacccatt aattgtgacg tcacctgtgg tgggcttttc tatgccactg atgaggttca gcaaagtgct tttaccactt ccactttgcc ccagcagcgc gacaaactca ccgggctcga aatcgaaggt gatgtggtct aggacttgtc gtcgggtttc gccttctcgg aatgtccagc tcaagttact cagctgaacg ataggttgcc ggttcatatg gtggtggtgg tggtgagcca t
Expected Protein Sequence
MAHHHHHHMN RQPIVQLSNL SWTFREGETR RQVLDHITFD FEPGEFVALL GQSGSGKSTL LNLISGIEKP TTGDVTINGF AITQKTERDR TLFRRDQIGI VFQFFNLIPT LTVLENITLP QELAGVSQRK AAVVARDLLE KVGMADRERT FPDKLSGGEQ QRVAISRALA HNPMLVLADE PTGNLDSDTG DKVLDVLLDL TRQAGKTLIM ATHSPSMTQH ADRVVNLQGG RLIPAVNREN QTDQPASTIL LPTSYE
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaacc ggcaacctat cgttcagctg agtaacttga gctggacatt ccgagaaggc gaaacccgac gacaagtcct agaccacatc accttcgatt tcgagcccgg tgagtttgtc gcgctgctgg ggcaaagtgg aagtggtaaa agcactttgc tgaacctcat cagtggcata gaaaagccca ccacaggtga cgtcacaatt aatgggttcg ctatcactca gaaaaccgag cgagaccgga cgttgttccg gcgcgatcag attggcatcg tctttcaatt tttcaacctg attcccactc ttaccgtgtt ggaaaatatt acgctgcctc aggaactggc cggagtttct cagaggaaag cggccgtggt cgctcgtgac cttctcgaaa aagtgggcat ggccgaccgt gaacgcacct ttcccgataa actctccggc ggagaacaac aacgggtcgc tatttccaga gcgttggcgc ataatcccat gctggtgtta gccgatgagc cgaccggcaa cctggactcc gataccgggg ataaagtctt ggatgttctg cttgatctca cccgccaagc aggtaaaacc ttaatcatgg ctacgcatag cccgtcgatg acgcagcatg ccgaccgggt agtcaactta cagggcggca ggttgatacc tgccgtgaac cgagaaaatc aaaccgacca gccggccagc acgatcctat tgcccacgtc atatgaatga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at