MythA.10014.b

Phosphonate ABC transporter ATPase subunit (Rv1747 ortholog)

CENTER ID: MythA.10014.b
ORGANISM: Mycobacterium thermoresistibile ATCC 19527 / NCTC 10409
ASSOCIATED DISEASE: Respiratory Infection
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MythA.10014.b.B1.GE37752 full length 1 275
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|1078020.3.peg.366
RefSeq: WP_003923796.1
UniProt: G7CBN3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTAPATGAPT APPDPTAART ATPGTSTAIE VTDLRVRFRR CRTDALAGVN LTVRQGETVA LIGTNGAGKS TLLRSLVRLV EPVGGRITLG DTDVTAASRR ELRTLRRDVG FVFQRFHLVP RLTAFHNVVH GAMGRRGTRC ASPLTAPGEV RREAMRALER VGLADHADRR VDTLSGGQQQ RVAVARMLMQ RPRIVLADEP VASLDPSSAN TVLELLTSVA TEGGVTVVMA LHQIDLALRY ADRVIGLRNG TLDFDQPARS CDTARLDPIF ARADT
NT Sequence
atgaccgccc cggccaccgg cgctcccacc gcgccgccgg atcccaccgc ggcgcgcacg gccacgcccg gcacgtccac tgcgatcgag gtgaccgacc tgcgggtgcg gttccgccgc tgtcgcaccg acgcgctggc cggcgtgaat ctgaccgtcc ggcagggcga aacggtcgcg ctgatcggca ccaacggcgc cggcaagtcg actctgctgc gctccctggt gcgtttggtc gaacccgtcg gcgggcggat cacgttgggc gacaccgatg tcaccgcggc gtcccggcgc gaactgcgga cactgcgccg cgatgtcgga ttcgtgttcc agcggttcca tctggtgccg cggctcaccg ccttccacaa tgtggtgcac ggggcgatgg gccggcgcgg cacccggtgc gcgtcaccgc tgaccgcacc gggggaggtc cggcgggagg cgatgcgcgc tctggagcgg gtggggctgg ccgatcacgc cgatcgccgg gtcgacacgc tcagcggcgg tcagcagcaa cgggtcgcgg tcgcacggat gctgatgcaa cgcccacgga tcgtgctcgc cgacgagccg gtcgccagcc tcgacccgag ttcggccaac accgtgctgg agctgctcac ctcggtcgcc accgaaggcg gagtgacggt ggtgatggcc ctgcaccaga tcgacctcgc cctgcggtat gccgaccggg tgatcgggtt gcgcaacggg acactggatt tcgatcaacc cgcccggtcg tgcgatacgg cacgactgga tccgatcttc gcgcgggcag acacatga
Details for MythA.10014.b.B1.GE37752
HARVESTED ON: 2/5/2014
SEQUENCED ON: 2/6/2014
EXPECTED MW: 31kDa
OBSERVED MW: 31kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 99
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHMTA PATGAPTAPP DPTAARTATP GTSTAIEVTD LRVRFRRCRT DALAGVNLTV RQGETVALIG TNGAGKSTLL RSLVRLVEPV GGRITLGDTD VTAASRRELR TLRRDVGFVF QRFHLVPRLT AFHNVVHGAM GRRGTRCASP LTAPGEVRRE AMRALERVGL ADHADRRVDT LSGGQQQRVA VARMLMQRPR IVLADEPVAS LDPSSANTVL ELLTSVATEG GVTVVMALHQ IDLALRYADR VIGLRNGTLD FDQPARSCDT ARLDPIFARA DT
Validated NT Sequence
cnnnnnnnnn ncctctanan nttttnttta actttaagaa ggagatatac catggctcac caccaccacc atatgaccgc cccggccacc ggcgctccca ccgcgccgcc ggatcccacc gcggcgcgca cggccacgcc cggcacgtcc actgcgatcg aggtgaccga cctgcgggtg cggttccgcc gctgtcgcac cgacgcgctg gccggcgtga atctgaccgt ccggcagggc gaaacggtcg cgctgatcgg caccaacggc gccggcaagt cgactctgct gcgctccctg gtgcgtttgg tcgaacccgt cggcgggcgg atcacgttgg gcgacaccga tgtcaccgcg gcgtcccggc gcgaactgcg gacactgcgc cgcgatgtcg gattcgtgtt ccagcggttc catctggtgc cgcggctcac cgccttccac aatgtggtgc acggggcgat gggccggcgc ggcacccggt gcgcgtcacc gctgaccgca ccgggggagg tccggcggga ggcgatgcgc gctctggagc gggtggggct ggccgatcac gccgatcgcc gggtcgacac gctcagcggc ggtcagcagc aacgggtcgc ggtcgcacgg atgctgatgc aacgcccacg gatcgtgctc gccgacgagc cggtcgccag cctcgacccg agttcggcca acaccgtgct ggagctgctc acctcggtcg ccaccgaagg cggagtgacg gtggtgatgg ccctgcacca gatcgacctc gccctgcggt atgccgaccg ggtgatcggg ttgcgcaacg ggacactgga tttcgatcaa cccgcccggt cgtgcgatac ggcacgactg gatccgatct tcgcgcgggc agacacataa gtgagtaaga taggatccgn ngnnnncccc cnnnnngnnn nnnnnnnnaa aaancnnnnn nnnnttttnt ccgagcnccc ctttcctcaa attcgtcntg nngnantatn cccccgcaga cct
Expected Protein Sequence
MAHHHHHHMT APATGAPTAP PDPTAARTAT PGTSTAIEVT DLRVRFRRCR TDALAGVNLT VRQGETVALI GTNGAGKSTL LRSLVRLVEP VGGRITLGDT DVTAASRREL RTLRRDVGFV FQRFHLVPRL TAFHNVVHGA MGRRGTRCAS PLTAPGEVRR EAMRALERVG LADHADRRVD TLSGGQQQRV AVARMLMQRP RIVLADEPVA SLDPSSANTV LELLTSVATE GGVTVVMALH QIDLALRYAD RVIGLRNGTL DFDQPARSCD TARLDPIFAR ADT
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaccg ccccggccac cggcgctccc accgcgccgc cggatcccac cgcggcgcgc acggccacgc ccggcacgtc cactgcgatc gaggtgaccg acctgcgggt gcggttccgc cgctgtcgca ccgacgcgct ggccggcgtg aatctgaccg tccggcaggg cgaaacggtc gcgctgatcg gcaccaacgg cgccggcaag tcgactctgc tgcgctccct ggtgcgtttg gtcgaacccg tcggcgggcg gatcacgttg ggcgacaccg atgtcaccgc ggcgtcccgg cgcgaactgc ggacactgcg ccgcgatgtc ggattcgtgt tccagcggtt ccatctggtg ccgcggctca ccgccttcca caatgtggtg cacggggcga tgggccggcg cggcacccgg tgcgcgtcac cgctgaccgc accgggggag gtccggcggg aggcgatgcg cgctctggag cgggtggggc tggccgatca cgccgatcgc cgggtcgaca cgctcagcgg cggtcagcag caacgggtcg cggtcgcacg gatgctgatg caacgcccac ggatcgtgct cgccgacgag ccggtcgcca gcctcgaccc gagttcggcc aacaccgtgc tggagctgct cacctcggtc gccaccgaag gcggagtgac ggtggtgatg gccctgcacc agatcgacct cgccctgcgg tatgccgacc gggtgatcgg gttgcgcaac gggacactgg atttcgatca acccgcccgg tcgtgcgata cggcacgact ggatccgatc ttcgcgcggg cagacacatg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga 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