SSGCID: Target Page

MytuD.01155.a

phosphoserine phosphatase

CENTER ID:	MytuD.01155.a
ORGANISM:	Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE:	Tuberculosis
CURRENT STATUS:	purified
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MytuD.01155.a.A1.GE25937	Full length( MytuD.01155.a )		1	409

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|83332.12.peg.3394
RefSeq:	NP_217558.1
TubercuList:	Rv3042c
MTB Network Portal:	Rv3042c
UniProt:	O53289

Orthologues for Rv3042c

Species	% Identity	% Coverage	Clones	Proteins	Structures
leprae	86	100	1	0	0
marinum	86	100	1	0	0
ulcerans	86	100	0	0	0
avium	84	100	1	1	1
paratuberculosis	84	100	1	0	0
smegmatis	82	99	0	0	0
abscessus	70	100	1	1	0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MPAKVSVLIT VTGMDQPGVT SALFEVLAQH GVELLNVEQV VIRGRLTLGV LVSCPLDVAD GTALRDDVAA AIHGVGLDVA IERSDDLPII RQPSTHTIFV LGRPITAGAF SAVARGVAAL GVNIDFIRGI SDYPVTGLEL RVSVPPGCVG PLQIALTKVA AEEHVDVAVE DYGLAWRTKR LIVFDVDSTL VQGEVIEMLA ARAGAQGQVA AITEAAMRGE LDFAESLQRR VATLAGLPAT VIDDVAEQLE LMPGARTTIR TLRRLGFRCG VVSGGFRRII EPLARELMLD FVASNELEIV DGILTGRVVG PIVDRPGKAK ALRDFASQYG VPMEQTVAVG DGANDIDMLG AAGLGIAFNA KPALREVADA SLSHPYLDTV LFLLGVTRGE IEAADAGDCG VRRVEIPAD

NT Sequence

ATGCCAGCCA AGGTGTCGGT GCTGATCACG GTCACCGGCA TGGATCAGCC AGGCGTGACA TCCGCGCTCT TCGAGGTGCT CGCGCAGCAC GGAGTCGAGC TGCTCAACGT CGAACAAGTG GTGATCCGGG GCCGGCTCAC GCTGGGTGTG CTGGTGTCGT GCCCGCTGGA CGTTGCCGAC GGTACCGCGC TGCGCGACGA CGTCGCGGCC GCCATCCACG GGGTGGGTCT AGACGTCGCC ATCGAGCGCA GCGACGATCT GCCAATCATT CGGCAACCGT CCACCCACAC CATTTTCGTG CTGGGACGCC CGATTACCGC CGGAGCGTTC AGCGCGGTGG CCAGGGGAGT GGCCGCACTC GGCGTCAACA TCGACTTCAT CCGCGGCATT TCCGACTACC CGGTCACCGG CCTGGAGTTG CGGGTCTCGG TGCCGCCTGG ATGCGTGGGT CCGCTGCAGA TCGCCCTGAC CAAGGTGGCC GCCGAAGAGC ATGTCGATGT GGCCGTCGAG GACTACGGCT TGGCTTGGCG CACCAAACGC CTGATCGTCT TCGACGTCGA CTCCACTCTG GTCCAAGGCG AGGTCATCGA GATGCTGGCA GCCCGTGCGG GTGCCCAGGG ACAGGTCGCC GCGATCACCG AGGCCGCGAT GCGTGGTGAG CTGGACTTCG CCGAGTCGCT GCAGCGTCGT GTTGCCACCT TGGCGGGGCT GCCCGCGACG GTGATAGACG ACGTCGCCGA GCAGCTCGAA CTGATGCCCG GTGCTCGGAC CACGATCCGG ACCTTGCGGC GTCTGGGTTT TCGCTGCGGT GTGGTTTCCG GCGGCTTTCG GCGGATCATC GAGCCGCTCG CACGCGAGTT GATGTTGGAT TTCGTTGCGT CCAACGAGCT GGAAATCGTC GATGGCATTC TTACCGGCCG GGTCGTGGGG CCGATTGTTG ACCGGCCCGG CAAGGCCAAG GCGCTTCGGG ACTTCGCGAG CCAGTATGGG GTGCCGATGG AGCAGACCGT CGCGGTCGGC GACGGCGCCA ACGATATCGA CATGCTGGGC GCGGCCGGGC TGGGAATAGC ATTCAACGCC AAGCCGGCGT TGCGTGAGGT CGCCGATGCG TCGTTGAGCC ATCCGTACCT GGACACGGTG TTGTTCTTGT TGGGCGTGAC TCGCGGTGAG ATCGAGGCCG CCGACGCCGG CGACTGTGGG GTGCGTCGCG TCGAAATCCC GGCCGAC

Details for MytuD.01155.a.A1.GE25937

HARVESTED ON:	1/9/2009
SEQUENCED ON:	4/11/2013
EXPECTED MW:	45kDa
OBSERVED MW:	46kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMPAKVSVLI TVTGMDQPGV TSALFEVLAQ HGVELLNVEQ VVIRGRLTLG VLVSCPLDVA DGTALRDDVA AAIHGVGLDV AIERSDDLPI IRQPSTHTIF VLGRPITAGA FSAVARGVAA LGVNIDFIRG ISDYPVTGLE LRVSVPPGCV GPLQIALTKV AAEEHVDVAV EDYGLAWRTK RLIVFDVDST LVQGEVIEML AARAGAQGQV AAITEAAMRG ELDFAESLQR RVATLAGLPA TVIDDVAEQL ELMPGARTTI RTLRRLGFRC GVVSGGFRRI IEPLARELML DFVASNELEI VDGILTGRVV GPIVDRPGKA KALRDFASQY GVPMEQTVAV GDGANDIDML GAAGLGIAFN AKPALREVAD ASLSHPYLDT VLFLLGVTRG EIEAADAGDC GVRRVEIPAD

Validated NT Sequence

ttaagaagga ganataccat ggctcatcac catcaccatc atatgggtac cctggaagct cagacccagg gtcctggttc gatgccagcc aaggtgtcgg tgctgatcac ggtcaccggc atggatcagc caggcgtgac atccgcgctc ttcgaggtgc tcgcgcagca cggagtcgag ctgctcaacg tcgaacaagt ggtgatccgg ggccggctca cgctgggtgt gctggtgtcg tgcccgctgg acgttgccga cggtaccgcg ctgcgcgacg acgtcgcggc cgccatccac ggggtgggtc tagacgtcgc catcgagcgc agcgacgatc tgccaatcat tcggcaaccg tccacccaca ccattttcgt gctgggacgc ccgattaccg ccggagcgtt cagcgcggtg gccaggggag tggccgcact cggcgtcaac atcgacttca tccgcggcat ttccgactac ccggtcaccg gcctggagtt gcgggtctcg gtgccgcctg gatgcgtggg tccgctgcag atcgccctga ccaaggtggc cgccgaagag catgtcgatg tggccgtcga ggactacggc ttggcttggc gcaccaaacg cctgatcgtc ttcgacgtcg actccactct ggtccaaggc gaggtcatcg agatgctggc agcccgtgcg ggtgcccagg gacaggtcgc cgcgatcacc gaggccgcga tgcgtggtga gctggacttc gccgagtcgc tgcagcgtcg tgttgccacc ttggcggggc tgcccgcgac ggtgatagac gacgtcgccg agcagctcga actgatgccc ggtgctcgga ccacgatccg gaccttgcgg cgtctgggtt ttcgctgcgg tgtggtttcc ggcggctttc ggcggatcat cgagccgctc gcacgcgagt tgatgttgga tttcgttgcg tccaacgagc tggaaatcgt cgatggcatt cttaccggcc gggtcgtggg gccgattgtt gaccggcccg gcaaggccaa ggcgcttcgg gacttcgcga gccagtatgg ggtgccgatg gagcagaccg tcgcggtcgg cgacggcgcc aacgatatcg acatgctggg cgcggccggg ctgggaatag cattcaacgc caagccggcg ttgcgtgagg tcgccgatgc gtcgttgagc catccgtacc tggacacggt gttgttcttg ttgggcgtga ctcgcggtga gatcgaggcc gccgacgccg gcgactgtgg ggtgcgtcgc gtcgaaatcc cggccgacta a

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gccagccaag gtgtcggtgc tgatcacggt caccggcatg gatcagccag gcgtgacatc cgcgctcttc gaggtgctcg cgcagcacgg agtcgagctg ctcaacgtcg aacaagtggt gatccggggc cggctcacgc tgggtgtgct ggtgtcgtgc ccgctggacg ttgccgacgg taccgcgctg cgcgacgacg tcgcggccgc catccacggg gtgggtctag acgtcgccat cgagcgcagc gacgatctgc caatcattcg gcaaccgtcc acccacacca ttttcgtgct gggacgcccg attaccgccg gagcgttcag cgcggtggcc aggggagtgg ccgcactcgg cgtcaacatc gacttcatcc gcggcatttc cgactacccg gtcaccggcc tggagttgcg ggtctcggtg ccgcctggat gcgtgggtcc gctgcagatc gccctgacca aggtggccgc cgaagagcat gtcgatgtgg ccgtcgagga ctacggcttg gcttggcgca ccaaacgcct gatcgtcttc gacgtcgact ccactctggt ccaaggcgag gtcatcgaga tgctggcagc ccgtgcgggt gcccagggac aggtcgccgc gatcaccgag gccgcgatgc gtggtgagct ggacttcgcc gagtcgctgc agcgtcgtgt tgccaccttg gcggggctgc ccgcgacggt gatagacgac gtcgccgagc agctcgaact gatgcccggt gctcggacca cgatccggac cttgcggcgt ctgggttttc gctgcggtgt ggtttccggc ggctttcggc ggatcatcga gccgctcgca cgcgagttga tgttggattt cgttgcgtcc aacgagctgg aaatcgtcga tggcattctt accggccggg tcgtggggcc gattgttgac cggcccggca aggccaaggc gcttcgggac ttcgcgagcc agtatggggt gccgatggag cagaccgtcg cggtcggcga cggcgccaac gatatcgaca tgctgggcgc ggccgggctg ggaatagcat tcaacgccaa gccggcgttg cgtgaggtcg ccgatgcgtc gttgagccat ccgtacctgg acacggtgtt gttcttgttg ggcgtgactc gcggtgagat cgaggccgcc gacgccggcg actgtggggt gcgtcgcgtc gaaatcccgg ccgacaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa