SSGCID: Target Page

MyleA.01155.a

Putative phosphoserine phosphatase

CENTER ID:	MyleA.01155.a
ORGANISM:	Mycobacterium leprae Br4923
ASSOCIATED DISEASE:	Hansen's disease, leprosy
CURRENT STATUS:	purified
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyleA.01155.a.A1.GE29256	Full length( MyleA.01155.a )		1	411

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|561304.4.peg.3262
RefSeq:	YP_002503819.1
UniProt:	A0A0H3MVN4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MNTPPKVSVL ITVTGVDQPG VTSALFAVLS RHGGELLNVE QVVIRGRLTL GVLVLCPPEV AAYTALRDDV QAAIRRVGLD VTIERSDDLP IIREPSTHTI FVLGRPITAR AFGAVAREVA ALDVNIDVIR GISDYPVTGL ELRVSAPLEA AGSLRTALNR VATEEHVDVA VEGYGLARRI KRLIVFDVDS TLVQGEVIEM LAARAGAQGT VAAITEAAMR GELDFAESLQ QRVATLAGLP VTVVDEVANQ LQLMPGARTT LRTLRRLGFR CGVVSGGFRR IIEPLAHELM LDFVAANELE IVDGILTGRV VGTIVDRPGK AKALRYFSEQ AGVPMEQTVA VGDGANDIDM LTAAGLGIAF NAKPALRAVA DASLSHPYLD TVLFVLGVTR GEIEAADAAD GGVRRVEIPP D

NT Sequence

GTGAATACGC CACCCAAGGT GTCGGTGCTG ATCACTGTCA CCGGCGTGGA TCAGCCAGGT GTGACGTCAG CGCTCTTCGC GGTGCTTTCG CGGCACGGGG GCGAACTGCT CAACGTCGAG CAGGTGGTAA TTCGGGGTCG GCTCACACTG GGTGTGCTGG TGTTGTGCCC GCCGGAAGTT GCCGCTTATA CCGCGTTGCG AGATGACGTG CAAGCCGCGA TTCGCCGGGT CGGCCTTGAT GTCACGATCG AGCGCAGTGA CGACTTGCCG ATCATCCGGG AACCGTCGAC CCACACGATT TTTGTGCTGG GCCGTCCGAT TACCGCTCGG GCGTTCGGCG CTGTAGCACG TGAGGTTGCG GCACTGGATG TCAATATCGA CGTCATTCGA GGCATCTCCG ACTACCCGGT TACCGGGCTG GAGTTGCGAG TCTCGGCGCC GTTGGAAGCC GCGGGATCGC TGCGGACAGC TCTCAACCGG GTGGCTACCG AGGAACATGT CGACGTGGCA GTGGAGGGTT ACGGTCTGGC CCGGCGCATC AAACGCCTCA TTGTGTTTGA CGTCGATTCC ACTTTGGTCC AGGGTGAGGT GATTGAAATG CTGGCCGCCC GAGCTGGCGC CCAGGGAACG GTCGCCGCGA TCACCGAGGC CGCGATGCGC GGTGAACTGG ATTTCGCGGA ATCGTTGCAG CAGCGGGTGG CCACCTTGGC GGGACTGCCT GTCACGGTGG TCGATGAGGT GGCCAATCAG TTGCAGCTGA TGCCAGGTGC TCGGACCACT CTGCGTACGC TGCGACGGCT GGGGTTTCGT TGCGGGGTGG TGTCCGGAGG CTTTCGGCGG ATCATCGAGC CGCTTGCGCA CGAGCTGATG CTGGACTTCG TCGCGGCCAA TGAGTTAGAG ATCGTCGACG GCATACTTAC TGGCCGGGTG GTCGGCACAA TCGTCGATCG GCCCGGTAAG GCCAAGGCGT TACGCTACTT CTCAGAGCAG GCTGGTGTGC CGATGGAGCA GACCGTTGCC GTCGGTGACG GCGCCAATGA CATCGACATG CTGACAGCGG CTGGGCTGGG AATTGCGTTT AACGCTAAGC CGGCGTTGCG TGCCGTCGCC GATGCGTCGT TGAGCCATCC CTACCTGGAC ACGGTGCTTT TTGTGTTGGG TGTGACACGC GGAGAGATCG AGGCCGCGGA CGCGGCCGAC GGCGGGGTGC GCCGCGTCGA GATCCCTCCC GAC

Details for MyleA.01155.a.A1.GE29256

HARVESTED ON:	4/13/2010
SEQUENCED ON:	4/24/2010
EXPECTED MW:	45kDa
OBSERVED MW:	46kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMVNTPPKVS VLITVTGVDQ PGVTSALFAV LSRHGGELLN VEQVVIRGRL TLGVLVLCPP EVAAYTALRD DVQAAIRRVG LDVTIERSDD LPIIREPSTH TIFVLGRPIT ARAFGAVARE VAALDVNIDV IRGISDYPVT GLELRVSAPL EAAGSLRTAL NRVATEEHVD VAVEGYGLAR RIKRLIVFDV DSTLVQGEVI EMLAARAGAQ GTVAAITEAA MRGELDFAES LQQRVATLAG LPVTVVDEVA NQLQLMPGAR TTLRTLRRLG FRCGVVSGGF RRIIEPLAHE LMLDFVAANE LEIVDGILTG RVVGTIVDRP GKAKALRYFS EQAGVPMEQT VAVGDGANDI DMLTAAGLGI AFNAKPALRA VADASLSHPY LDTVLFVLGV TRGEIEAADA ADGGVRRVEI PPD

Validated NT Sequence

atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggtga atacgccacc caaggtgtcg gtgctgatca ctgtcaccgg cgtggatcag ccaggtgtga cgtcagcgct cttcgcggtg ctttcgcggc acgggggcga actgctcaac gtcgagcagg tggtaattcg gggtcggctc acactgggtg tgctggtgtt gtgcccgccg gaagttgccg cttataccgc gttgcgagat gacgtgcaag ccgcgattcg ccgggtcggc cttgatgtca cgatcgagcg cagtgacgac ttgccgatca tccgggaacc gtcgacccac acgatttttg tgctgggccg tccgattacc gctcgggcgt tcggcgctgt agcacgtgag gttgcggcac tggatgtcaa tatcgacgtc attcgaggca tctccgacta cccggttacc gggctggagt tgcgagtctc ggcgccgttg gaagccgcgg gatcgctgcg gacagctctc aaccgggtgg ctaccgagga acatgtcgac gtggcagtgg agggttacgg tctggcccgg cgcatcaaac gcctcattgt gtttgacgtc gattccactt tggtccaggg tgaggtgatt gaaatgctgg ccgcccgagc tggcgcccag ggaacggtcg ccgcgatcac cgaggccgcg atgcgcggtg aactggattt cgcggaatcg ttgcagcagc gggtggccac cttggcggga ctgcctgtca cggtggtcga tgaggtggcc aatcagttgc agctgatgcc aggtgctcgg accactctgc gtacgctgcg acggctgggg tttcgttgcg gggtggtgtc cggaggcttt cggcggatca tcgagccgct tgcgcacgag ctgatgctgg acttcgtcgc ggccaatgag ttagagatcg tcgacggcat acttactggc cgggtggtcg gcacaatcgt cgatcggccc ggtaaggcca aggcgttacg ctacttctca gagcaggctg gtgtgccgat ggagcagacc gttgccgtcg gtgacggcgc caatgacatc gacatgctga cagcggctgg gctgggaatt gcgtttaacg ctaagccggc gttgcgtgcc gtcgccgatg cgtcgttgag ccatccctac ctggacacgg tgctttttgt gttgggtgtg acacgcggag agatcgaggc cgcggacgcg gccgacggcg gggtgcgccg cgtcgagatc cctcccgact aataa

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgaatacg ccacccaagg tgtcggtgct gatcactgtc accggcgtgg atcagccagg tgtgacgtca gcgctcttcg cggtgctttc gcggcacggg ggcgaactgc tcaacgtcga gcaggtggta attcggggtc ggctcacact gggtgtgctg gtgttgtgcc cgccggaagt tgccgcttat accgcgttgc gagatgacgt gcaagccgcg attcgccggg tcggccttga tgtcacgatc gagcgcagtg acgacttgcc gatcatccgg gaaccgtcga cccacacgat ttttgtgctg ggccgtccga ttaccgctcg ggcgttcggc gctgtagcac gtgaggttgc ggcactggat gtcaatatcg acgtcattcg aggcatctcc gactacccgg ttaccgggct ggagttgcga gtctcggcgc cgttggaagc cgcgggatcg ctgcggacag ctctcaaccg ggtggctacc gaggaacatg tcgacgtggc agtggagggt tacggtctgg cccggcgcat caaacgcctc attgtgtttg acgtcgattc cactttggtc cagggtgagg tgattgaaat gctggccgcc cgagctggcg cccagggaac ggtcgccgcg atcaccgagg ccgcgatgcg cggtgaactg gatttcgcgg aatcgttgca gcagcgggtg gccaccttgg cgggactgcc tgtcacggtg gtcgatgagg tggccaatca gttgcagctg atgccaggtg ctcggaccac tctgcgtacg ctgcgacggc tggggtttcg ttgcggggtg gtgtccggag gctttcggcg gatcatcgag ccgcttgcgc acgagctgat gctggacttc gtcgcggcca atgagttaga gatcgtcgac ggcatactta ctggccgggt ggtcggcaca atcgtcgatc ggcccggtaa ggccaaggcg ttacgctact tctcagagca ggctggtgtg ccgatggagc agaccgttgc cgtcggtgac ggcgccaatg acatcgacat gctgacagcg gctgggctgg gaattgcgtt taacgctaag ccggcgttgc gtgccgtcgc cgatgcgtcg ttgagccatc cctacctgga cacggtgctt tttgtgttgg gtgtgacacg cggagagatc gaggccgcgg acgcggccga cggcggggtg cgccgcgtcg agatccctcc cgacaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa