MytuD.01058.a

hypothetical protein

CENTER ID: MytuD.01058.a
ORGANISM: Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE: Tuberculosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MytuD.01058.a.B1.GE27174 Full length( MytuD.01058.a ) 1 307
MytuD.01058.a.B1.GE27593 Full length( MytuD.01058.a ) 1 307
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|83332.12.peg.1519
RefSeq: NP_215873.1
TubercuList: Rv1357c
MTB Network Portal: Rv1357c
UniProt: P9WM07

Orthologues for Rv1357c

Species % Identity % Coverage Clones Proteins Structures
leprae 73 86 1 0 0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MDRCCQRATA FACALRPTKL IDYEEMFRGA MQARAMVANP DQWADSDRDQ VNTRHYLSTS MRVALDRGEF FLVYQPIIRL ADNRIIGAEA LLRWEHPTLG TLLPGRFIDR AENNGLMVPL TAFVLEQACR HVRSWRDHST DPQPFVSVNV SASTICDPGF LVLVEGVLGE TGLPAHALQL ELAEDARLSR DEKAVTRLQE LSALGVGIAI DDFGIGFSSL AYLPRLPVDV VKLGGKFIEC LDGDIQARLA NEQITRAMID LGDKLGITVT AKLVETPSQA ARLRAFGCKA AQGWHFAKAL PVDFFRE
NT Sequence
ATGGATCGTT GTTGTCAGCG CGCTACAGCG TTCGCTTGCG CGCTCAGGCC GACCAAGTTG ATCGACTACG AAGAGATGTT TAGGGGCGCG ATGCAAGCGC GAGCGATGGT AGCCAATCCT GACCAATGGG CGGACTCCGA CCGCGACCAG GTCAACACTC GCCATTATCT GTCCACTTCG ATGCGCGTGG CACTGGATCG CGGTGAATTC TTCCTCGTCT ACCAGCCAAT CATCCGGCTT GCCGACAACC GCATCATCGG CGCCGAGGCC CTGCTGCGCT GGGAACACCC GACGTTGGGC ACGCTACTCC CGGGCCGGTT CATCGACCGT GCCGAGAACA ACGGACTGAT GGTGCCGCTC ACGGCCTTCG TGCTCGAGCA GGCCTGCCGC CACGTCCGCA GTTGGCGTGA CCACAGCACC GACCCGCAAC CGTTTGTCAG CGTCAACGTC TCCGCCAGCA CCATCTGCGA TCCCGGCTTC CTGGTGCTGG TCGAAGGTGT GCTCGGCGAA ACCGGCCTGC CCGCCCATGC CCTGCAGCTC GAACTGGCCG AGGACGCGCG CCTTAGCAGA GACGAGAAGG CGGTGACCAG GCTACAAGAA TTGTCCGCTC TCGGCGTCGG CATCGCCATC GACGACTTCG GCATTGGATT CTCCAGCCTC GCCTACCTTC CCCGCCTCCC CGTCGACGTG GTCAAACTCG GGGGAAAGTT CATCGAGTGC CTCGATGGCG ACATTCAAGC TCGGCTGGCC AACGAACAGA TCACCCGGGC AATGATCGAC CTTGGCGACA AGCTCGGTAT CACCGTCACT GCAAAGCTAG TCGAAACCCC CAGCCAAGCC GCCCGGTTGC GCGCCTTCGG CTGTAAAGCC GCACAAGGCT GGCACTTTGC CAAGGCACTG CCGGTCGACT TTTTCAGAGA G
Details for MytuD.01058.a.B1.GE27174
HARVESTED ON: 7/20/2009
SEQUENCED ON: 7/28/2009
EXPECTED MW: 35kDa
OBSERVED MW: 35kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMD RCCQRATAFA CALRPTKLID YEEMFRGAMQ ARAMVANPDQ WADSDRDQVN TRHYLSTSMR VALDRGEFFL VYQPIIRLAD NRIIGAEALL RWEHPTLGTL LPGRFIDRAE NNGLMVPLTA FVLEQACRHV RSWRDHSTDP QPFVSVNVSA STICDPGFLV LVEGVLGETG LPAHALQLEL AEDARLSRDE KAVTRLQELS ALGVGIAIDD FGIGFSSLAY LPRLPVDVVK LGGKFIECLD GDIQARLANE QITRAMIDLG DKLGITVTAK LVETPSQAAR LRAFGCKAAQ GWHFAKALPV DFFRE
Validated NT Sequence
tcttactcac ttactctctg aaaaagtcga ccggcagtgc cttggcaaag tgccagcctt gtgcggcttt acagccgaag gcgcgcaacc gggcggcttg gctgggggtt tcgactagct ttgcagtgac ggtgataccg agcttgtcgc caaggtcgat cattgcccgg gtgatctgtt cgttggccag ccgagcttga atgtcgccat cgaggcactc gatgaacttt cccccgagtt tgaccacgtc gacggggagg cggggaaggt aggcgaggct ggagaatcca atgccgaagt cgtcgatggc gatgccgacg ccgagagcgg acaattcttg tagcctggtc accgccttct cgtctctgct aaggcgcgcg tcctcggcca gttcgagctg cagggcatgg gcgggcaggc cggtttcgcc gagcacacct tcgaccagca ccaggaagcc gggatcgcag atggtgctgg cggagacgtt gacgctgaca aacggttgcg ggtcggtgct gtggtcacgc caactgcgga cgtggcggca ggcctgctcg agcacgaagg ccgtgagcgg caccatcagt ccgttgttct cggcacggtc gatgaaccgg cccgggagta gcgtgcccaa cgtcgggtgt tcccagcgca gcagggcctc ggcgccgatg atgcggttgt cggcaagccg gatgattggc tggtagacga ggaagaattc accgcgatcc agtgccacgc gcatcgaagt ggacagataa tggcgagtgt tgacctggtc gcggtcggag tccgcccatt ggtcaggatt ggctaccatc gctcgcgctt gcatcgcgcc cctaaacatc tcttcgtagt cgatcaactt ggtcggcctg agcgcgcaag cgaacgctgt agcgcgctga caacaacgat ccatatggtg gtggtggtgg tgagccatgg tatnnctcct t
Expected Protein Sequence
MAHHHHHHMD RCCQRATAFA CALRPTKLID YEEMFRGAMQ ARAMVANPDQ WADSDRDQVN TRHYLSTSMR VALDRGEFFL VYQPIIRLAD NRIIGAEALL RWEHPTLGTL LPGRFIDRAE NNGLMVPLTA FVLEQACRHV RSWRDHSTDP QPFVSVNVSA STICDPGFLV LVEGVLGETG LPAHALQLEL AEDARLSRDE KAVTRLQELS ALGVGIAIDD FGIGFSSLAY LPRLPVDVVK LGGKFIECLD GDIQARLANE QITRAMIDLG DKLGITVTAK LVETPSQAAR LRAFGCKAAQ GWHFAKALPV DFFRE
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggatc gttgttgtca gcgcgctaca gcgttcgctt gcgcgctcag gccgaccaag ttgatcgact acgaagagat gtttaggggc gcgatgcaag cgcgagcgat ggtagccaat cctgaccaat gggcggactc cgaccgcgac caggtcaaca ctcgccatta tctgtccact tcgatgcgcg tggcactgga tcgcggtgaa ttcttcctcg tctaccagcc aatcatccgg cttgccgaca accgcatcat cggcgccgag gccctgctgc gctgggaaca cccgacgttg ggcacgctac tcccgggccg gttcatcgac cgtgccgaga acaacggact gatggtgccg ctcacggcct tcgtgctcga gcaggcctgc cgccacgtcc gcagttggcg tgaccacagc accgacccgc aaccgtttgt cagcgtcaac gtctccgcca gcaccatctg cgatcccggc ttcctggtgc tggtcgaagg tgtgctcggc gaaaccggcc tgcccgccca tgccctgcag ctcgaactgg ccgaggacgc gcgccttagc agagacgaga aggcggtgac caggctacaa gaattgtccg ctctcggcgt cggcatcgcc atcgacgact tcggcattgg attctccagc ctcgcctacc ttccccgcct ccccgtcgac gtggtcaaac tcgggggaaa gttcatcgag tgcctcgatg gcgacattca agctcggctg gccaacgaac agatcacccg ggcaatgatc gaccttggcg acaagctcgg tatcaccgtc actgcaaagc tagtcgaaac ccccagccaa gccgcccggt tgcgcgcctt cggctgtaaa gccgcacaag gctggcactt tgccaaggca ctgccggtcg actttttcag agagtgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat
Details for MytuD.01058.a.B1.GE27593
HARVESTED ON: 8/17/2009
SEQUENCED ON: 9/29/2009
EXPECTED MW: 35kDa
OBSERVED MW: 35kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMD RCCQRATAFA CALRPTKLID YEEMFRGAMQ ARAMVANPDQ WADSDRDQVN TRHYLSTSMR VALDRGEFFL VYQPIIRLAD NRIIGAEALL RWEHPTLGTL LPGRFIDRAE NNGLMVPLTA FVLEQACRHV RSWRDHSTDP QPFVSVNVSA STICDPGFLV LVEGVLGETG LPAHALQLEL AEDARLSRDE KAVTRLQELS ALGVGIAIDD FGIGFSSLAY LPRLPVDVVK LGGKFIECLD GDIQARLANE QITRAMIDLG DKLGITVTAK LVETPSQAAR LRAFGCKAAQ GWHFAKALPV DFFRE
Validated NT Sequence
tcttactcac ttactctctg aaaaagtcga ccggcagtgc cttggcaaag tgccagcctt gtgcggcttt acagccgaag gcgcgcaacc gggcggcttg gctgggggtt tcgactagct ttgcagtgac ggtgataccg agcttgtcgc caaggtcgat cattgcccgg gtgatctgtt cgttggccag ccgagcttga atgtcgccat cgaggcactc gatgaacttt cccccgagtt tgaccacgtc gacggggagg cggggaaggt aggcgaggct ggagaatcca atgccgaagt cgtcgatggc gatgccgacg ccgagagcgg acaattcttg tagcctggtc accgccttct cgtctctgct aaggcgcgcg tcctcggcca gttcgagctg cagggcatgg gcgggcaggc cggtttcgcc gagcacacct tcgaccagca ccaggaagcc gggatcgcag atggtgctgg cggagacgtt gacgctgaca aacggttgcg ggtcggtgct gtggtcacgc caactgcgga cgtggcggca ggcctgctcg agcacgaagg ccgtgagcgg caccatcagt ccgttgttct cggcacggtc gatgaaccgg cccgggagta gcgtgcccaa cgtcgggtgt tcccagcgca gcagggcctc ggcgccgatg atgcggttgt cggcaagccg gatgattggc tggtagacga ggaagaattc accgcgatcc agtgccacgc gcatcgaagt ggacagataa tggcgagtgt tgacctggtc gcggtcggag tccgcccatt ggtcaggatt ggctaccatc gctcgcgctt gcatcgcgcc cctaaacatc tcttcgtagt cgatcaactt ggtcggcctg agcgcgcaag cgaacgctgt agcgcgctga caacaacgat ccatatggtg gtggtggtgg tgagccatgg tatnnctcct t
Expected Protein Sequence
MAHHHHHHMD RCCQRATAFA CALRPTKLID YEEMFRGAMQ ARAMVANPDQ WADSDRDQVN TRHYLSTSMR VALDRGEFFL VYQPIIRLAD NRIIGAEALL RWEHPTLGTL LPGRFIDRAE NNGLMVPLTA FVLEQACRHV RSWRDHSTDP QPFVSVNVSA STICDPGFLV LVEGVLGETG LPAHALQLEL AEDARLSRDE KAVTRLQELS ALGVGIAIDD FGIGFSSLAY LPRLPVDVVK LGGKFIECLD GDIQARLANE QITRAMIDLG DKLGITVTAK LVETPSQAAR LRAFGCKAAQ GWHFAKALPV DFFRE
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggatc gttgttgtca gcgcgctaca gcgttcgctt gcgcgctcag gccgaccaag ttgatcgact acgaagagat gtttaggggc gcgatgcaag cgcgagcgat ggtagccaat cctgaccaat gggcggactc cgaccgcgac caggtcaaca ctcgccatta tctgtccact tcgatgcgcg tggcactgga tcgcggtgaa ttcttcctcg tctaccagcc aatcatccgg cttgccgaca accgcatcat cggcgccgag gccctgctgc gctgggaaca cccgacgttg ggcacgctac tcccgggccg gttcatcgac cgtgccgaga acaacggact gatggtgccg ctcacggcct tcgtgctcga gcaggcctgc cgccacgtcc gcagttggcg tgaccacagc accgacccgc aaccgtttgt cagcgtcaac gtctccgcca gcaccatctg cgatcccggc ttcctggtgc tggtcgaagg tgtgctcggc gaaaccggcc tgcccgccca tgccctgcag ctcgaactgg ccgaggacgc gcgccttagc agagacgaga aggcggtgac caggctacaa gaattgtccg ctctcggcgt cggcatcgcc atcgacgact tcggcattgg attctccagc ctcgcctacc ttccccgcct ccccgtcgac gtggtcaaac tcgggggaaa gttcatcgag tgcctcgatg gcgacattca agctcggctg gccaacgaac agatcacccg ggcaatgatc gaccttggcg acaagctcgg tatcaccgtc actgcaaagc tagtcgaaac ccccagccaa gccgcccggt tgcgcgcctt cggctgtaaa gccgcacaag gctggcactt tgccaaggca ctgccggtcg actttttcag agagtgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat