MyleA.01058.a

Putative uncharacterized protein

CENTER ID: MyleA.01058.a
ORGANISM: Mycobacterium leprae Br4923
ASSOCIATED DISEASE: Hansen's disease, leprosy
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyleA.01058.a.A1.GE29246 Full length( MyleA.01058.a ) 1 302
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|561304.4.peg.3319
RefSeq: YP_002503831.1
UniProt: A0A0H3MRB6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MPIEGAMRSS SLPDGCSDRS ADSRGSGEVK DRSSGWFYGH HDREQRSVHD GLLQVIPAAL DRGEFFLVYQ PIVRLNDKRF IGVEALLRWA HPTLGTLLPA RFISLAERNG MIVPLTTFVI EQACRHIRNW RDDSTNPRPF VSVNVSARNI RDPSFLSMVE RVLGETGLPA HALRLELTEN ASLSIDEASI TRLQELSALG VGIAIDDFGT GFSSLAYLRN LPVDMVKLAG EFIENLGGDI RGRLADEQIT RAMIDLAFKL GIIVCAKRVE TPGQAARLRD FGCDAAQGWH FAKALPADFF TE
NT Sequence
TTGCCTATCG AGGGTGCTAT GCGGAGCAGT TCGCTGCCGG ATGGGTGTAG TGACAGGTCA GCCGATTCGC GCGGCAGTGG CGAGGTAAAA GACCGAAGTA GTGGATGGTT TTACGGCCAT CACGATCGCG AGCAGCGGAG CGTCCACGAT GGTCTGTTGC AGGTGATTCC TGCTGCACTG GATCGCGGAG AATTTTTCCT CGTCTACCAG CCGATTGTCC GGCTTAATGA CAAACGCTTC ATCGGCGTCG AGGCGTTACT GCGTTGGGCG CATCCGACGC TGGGTACCCT ACTCCCAGCG CGATTCATCA GCCTTGCCGA GCGAAACGGC ATGATAGTAC CACTCACTAC CTTCGTGATT GAGCAGGCCT GCCGACATAT CCGCAACTGG CGTGACGACA GCACCAATCC GCGACCGTTC GTCAGCGTCA ACGTCTCGGC CCGCAACATT CGTGATCCCA GTTTTCTCTC GATGGTCGAG CGGGTGCTTG GCGAAACTGG TCTGCCTGCC CACGCGTTGC GGCTCGAACT CACTGAGAAC GCGAGCCTGA GCATAGATGA GGCATCGATC ACCAGGCTGC AGGAGCTGTC CGCGTTAGGC GTGGGTATCG CGATCGACGA CTTCGGTACT GGATTTTCCA GCCTCGCTTA TTTACGCAAC TTACCCGTCG ACATGGTCAA ACTCGCCGGC GAGTTCATCG AGAACCTCGG TGGTGATATC CGTGGCCGGT TGGCTGATGA ACAGATCACC CGGGCAATGA TTGACCTTGC CTTCAAACTT GGGATCATTG TTTGCGCCAA GCGAGTTGAA ACGCCCGGTC AAGCTGCTAG GTTGCGTGAT TTCGGCTGCG ACGCTGCCCA AGGCTGGCAT TTTGCGAAAG CGCTACCGGC CGATTTTTTC ACTGAG
Details for MyleA.01058.a.A1.GE29246
HARVESTED ON: 4/13/2010
SEQUENCED ON: 4/24/2010
EXPECTED MW: 35kDa
OBSERVED MW: 35kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMLPIEGAMR SSSLPDGCSD RSADSRGSGE VKDRSSGWFY GHHDREQRSV HDGLLQVIPA ALDRGEFFLV YQPIVRLNDK RFIGVEALLR WAHPTLGTLL PARFISLAER NGMIVPLTTF VIEQACRHIR NWRDDSTNPR PFVSVNVSAR NIRDPSFLSM VERVLGETGL PAHALRLELT ENASLSIDEA SITRLQELSA LGVGIAIDDF GTGFSSLAYL RNLPVDMVKL AGEFIENLGG DIRGRLADEQ ITRAMIDLAF KLGIIVCAKR VETPGQAARL RDFGCDAAQG WHFAKALPAD FFTE
Validated NT Sequence
ttattactca gtgaaaaaat cggccggtag cgctttcgca aaatgccagc cttgggcagc gtcgcagccg aaatcacgca acctagcagc ttgaccgggc gtttcaactc gcttggcgca aacaatgatc ccaagtttga aggcaaggtc aatcattgcc cgggtgatct gttcatcagc caaccggcca cggatatcac caccgaggtt ctcgatgaac tcgccggcga gtttgaccat gtcgacgggt aagttgcgta aataagcgag gctggaaaat ccagtaccga agtcgtcgat cgcgataccc acgcctaacg cggacagctc ctgcagcctg gtgatcgatg cctcatctat gctcaggctc gcgttctcag tgagttcgag ccgcaacgcg tgggcaggca gaccagtttc gccaagcacc cgctcgacca tcgagagaaa actgggatca cgaatgttgc gggccgagac gttgacgctg acgaacggtc gcggattggt gctgtcgtca cgccagttgc ggatatgtcg gcaggcctgc tcaatcacga aggtagtgag tggtactatc atgccgtttc gctcggcaag gctgatgaat cgcgctggga gtagggtacc cagcgtcgga tgcgcccaac gcagtaacgc ctcgacgccg atgaagcgtt tgtcattaag ccggacaatc ggctggtaga cgaggaaaaa ttctccgcga tccagtgcag caggaatcac ctgcaacaga ccatcgtgga cgctccgctg ctcgcgatcg tgatggccgt aaaaccatcc actacttcgg tcttttacct cgccactgcc gcgcgaatcg gctgacctgt cactacaccc atccggcagc gaactgctcc gcatagcacc ctcgataggc aacatcgaac caggaccctg ggtctgagct tccagggtac ccatatgatg gtgatggtga tgagccat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMLPIEGAMR SSSLPDGCSD RSADSRGSGE VKDRSSGWFY GHHDREQRSV HDGLLQVIPA ALDRGEFFLV YQPIVRLNDK RFIGVEALLR WAHPTLGTLL PARFISLAER NGMIVPLTTF VIEQACRHIR NWRDDSTNPR PFVSVNVSAR NIRDPSFLSM VERVLGETGL PAHALRLELT ENASLSIDEA SITRLQELSA LGVGIAIDDF GTGFSSLAYL RNLPVDMVKL AGEFIENLGG DIRGRLADEQ ITRAMIDLAF KLGIIVCAKR VETPGQAARL RDFGCDAAQG WHFAKALPAD FFTE
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gttgcctatc gagggtgcta tgcggagcag ttcgctgccg gatgggtgta gtgacaggtc agccgattcg cgcggcagtg gcgaggtaaa agaccgaagt agtggatggt tttacggcca tcacgatcgc gagcagcgga gcgtccacga tggtctgttg caggtgattc ctgctgcact ggatcgcgga gaatttttcc tcgtctacca gccgattgtc cggcttaatg acaaacgctt catcggcgtc gaggcgttac tgcgttgggc gcatccgacg ctgggtaccc tactcccagc gcgattcatc agccttgccg agcgaaacgg catgatagta ccactcacta ccttcgtgat tgagcaggcc tgccgacata tccgcaactg gcgtgacgac agcaccaatc cgcgaccgtt cgtcagcgtc aacgtctcgg cccgcaacat tcgtgatccc agttttctct cgatggtcga gcgggtgctt ggcgaaactg gtctgcctgc ccacgcgttg cggctcgaac tcactgagaa cgcgagcctg agcatagatg aggcatcgat caccaggctg caggagctgt ccgcgttagg cgtgggtatc gcgatcgacg acttcggtac tggattttcc agcctcgctt atttacgcaa cttacccgtc gacatggtca aactcgccgg cgagttcatc gagaacctcg gtggtgatat ccgtggccgg ttggctgatg aacagatcac ccgggcaatg attgaccttg ccttcaaact tgggatcatt gtttgcgcca agcgagttga aacgcccggt caagctgcta ggttgcgtga tttcggctgc gacgctgccc aaggctggca ttttgcgaaa gcgctaccgg ccgatttttt cactgagaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa