MytuD.00774.a

phosphoribosylglycinamide formyltransferase 2

CENTER ID: MytuD.00774.a
ORGANISM: Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE: Tuberculosis
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MytuD.00774.a.A1.GE25905 Full length( MytuD.00774.a ) 1 419
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MytuD.00774.a.A1.PS00210 Full length( MytuD.00774.a ) 1 419

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|83332.12.peg.428
RefSeq: NP_214903.1
TubercuList: Rv0389
MTB Network Portal: Rv0389
UniProt: P95197

Orthologues for Rv0389

Species % Identity % Coverage Clones Proteins Structures
paratuberculosis 64 94 0 0 0
marinum 63 100 1 1 0
avium 61 99 0 0 0
smegmatis 55 92 0 0 0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MIDGWTEEQH EPTVRHERPA APQDVRRVML LGSAEPSREL AIALQGLGAE VIAVDGYVGA PAHRIADQSV VVTMTDAEEL TAVIRRLQPD FLVTVTAAVS VDALDAVEQA DGECTELVPN ARAVRCTADR EGLRRLAADQ LGLPTAPFWF VGSLGELQAV AVHAGFPLLV SPVAGVAGQG SSVVAGPNEV EPAWQRAAGH QVQPQTGGVS PRVCAESVVE IEFLVTMIVV CSQGPNGPLI EFCAPIGHRD ADAGELESWQ PQKLSTAALD AAKSIAARIV KALGGRGVFG VELMINGDEV YFADVTVCPA GSAWVTVRSQ RLSVFELQAR AILGLAVDTL MISPGAARVI NPDHTAGRAA VGAAPPADAL TGALGVPESD VVIFGRGLGV ALATAPEVAI ARERAREVAS RLNVPDSRE
NT Sequence
GTGATCGACG GCTGGACGGA AGAACAGCAC GAACCCACCG TTAGGCATGA GCGCCCAGCA GCTCCCCAAG ACGTTCGGCG GGTGATGTTG CTGGGTTCGG CCGAACCCAG CCGGGAGCTG GCGATCGCGT TGCAGGGCTT GGGCGCGGAG GTGATCGCCG TCGACGGCTA TGTCGGCGCG CCTGCCCACC GGATAGCCGA CCAGTCGGTG GTGGTCACCA TGACCGATGC TGAAGAGCTG ACGGCGGTGA TCCGGCGGCT GCAACCGGAT TTCTTGGTGA CGGTCACCGC CGCGGTGTCT GTGGATGCTC TCGATGCCGT CGAGCAAGCC GACGGCGAGT GCACTGAGCT GGTGCCGAAC GCCCGTGCCG TCCGGTGCAC GGCCGACCGG GAGGGCCTGC GCCGGCTGGC CGCCGATCAG CTCGGCCTGC CCACAGCCCC GTTCTGGTTC GTCGGATCCC TTGGCGAACT TCAAGCGGTG GCCGTCCATG CTGGGTTTCC GTTGCTGGTG AGCCCGGTGG CAGGGGTGGC TGGCCAGGGT AGCTCGGTGG TCGCCGGGCC CAACGAGGTC GAGCCCGCCT GGCAGCGCGC GGCAGGCCAT CAAGTACAGC CGCAGACTGG GGGAGTGAGC CCTCGGGTGT GCGCCGAGTC GGTGGTCGAG ATCGAGTTTT TGGTCACCAT GATCGTTGTG TGCAGTCAGG GCCCGAACGG GCCGCTCATC GAGTTCTGTG CACCTATCGG TCATCGCGAC GCCGATGCCG GTGAGTTGGA ATCCTGGCAA CCGCAGAAGC TGAGCACGGC GGCGCTGGAC GCGGCCAAGT CGATCGCCGC GCGCATCGTC AAGGCGCTCG GGGGACGCGG GGTTTTCGGC GTCGAATTGA TGATCAACGG CGATGAGGTG TATTTCGCCG ATGTCACCGT GTGTCCTGCC GGGAGTGCCT GGGTCACCGT GCGCAGCCAG CGGCTTTCGG TGTTCGAACT GCAGGCCCGG GCGATCCTGG GTCTGGCGGT GGACACCCTG ATGATCTCGC CGGGTGCCGC GCGGGTGATC AACCCGGACC ACACGGCAGG CCGGGCAGCG GTCGGCGCCG CACCACCTGC CGATGCGCTG ACCGGTGCGC TCGGTGTGCC GGAAAGCGAC GTCGTGATAT TCGGCCGCGG GCTTGGGGTG GCGCTGGCCA CCGCACCCGA GGTGGCAATC GCCCGCGAAC GCGCCCGCGA AGTTGCATCT CGGCTAAATG TGCCAGACTC ACGCGAG
Details for MytuD.00774.a.A1.GE25905
HARVESTED ON: 1/9/2009
SEQUENCED ON: 8/18/2010
EXPECTED MW: 46kDa
OBSERVED MW: 47kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVIDGWTEE QHEPTVRHER PAAPQDVRRV MLLGSAEPSR ELAIALQGLG AEVIAVDGYV GAPAHRIADQ SVVVTMTDAE ELTAVIRRLQ PDFLVTVTAA VSVDALDAVE QADGECTELV PNARAVRCTA DREGLRRLAA DQLGLPTAPF WFVGSLGELQ AVAVHAGFPL LVSPVAGVAG QGSSVVAGPN EVEPAWQRAA GHQVQPQTGG VSPRVCAESV VEIEFLVTMI VVCSQGPNGP LIEFCAPIGH RDADAGELES WQPQKLSTAA LDAAKSIAAR IVKALGGRGV FGVELMINGD EVYFADVTVC PAGSAWVTVR SQRLSVFELQ ARAILGLAVD TLMISPGAAR VINPDHTAGR AAVGAAPPAD ALTGALGVPE SDVVIFGRGL GVALATAPEV AIARERAREV ASRLNVPDSR E
Validated NT Sequence
ttattactcg cgtgagtctg gcacatttag ccgagatgca acttcgcggg cgcgttcgcg ggcgattgcc acctcgggtg cggtggccag cgccacccca agcccgcggc cgaatatcac gacgtcgctt tccggcacac cgagcgcacc ggtcagcgca tcggcaggtg gtgcggcgcc gaccgctgcc cggcctgccg tgtggtccgg gttgatcacc cgcgcggcac ccggcgagat catcagggtg tccaccgcca gacccaggat cgcccgggcc tgcagttcga acaccgaaag ccgctggctg cgcacggtga cccaggcact cccggcagga cacacggtga catcggcgaa atacacctca tcgccgttga tcatcaattc gacgccgaaa accccgcgtc ccccgagcgc cttgacgatg cgcgcggcga tcgacttggc cgcgtccagc gccgccgtgc tcagcttctg cggttgccag gattccaact caccggcatc ggcgtcgcga tgaccgatag gtgcacagaa ctcgatgagc ggcccgttcg ggccctgact gcacacaacg atcatggtga ccaaaaactc gatctcgacc accgactcgg cgcacacccg agggctcact cccccagtct gcggctgtac ttgatggcct gccgcgcgct gccaggcggg ctcgacctcg ttgggcccgg cgaccaccga gctaccctgg ccagccaccc ctgccaccgg gctcaccagc aacggaaacc cagcatggac ggccaccgct tgaagttcgc caagggatcc gacgaaccag aacggggctg tgggcaggcc gagctgatcg gcggccagcc ggcgcaggcc ctcccggtcg gccgtgcacc ggacggcacg ggcgttcggc accagctcag tgcactcgcc gtcggcttgc tcgacggcat cgagagcatc cacagacacc gcggcggtga ccgtcaccaa gaaatccggt tgcagccgcc ggatcaccgc cgtcagctct tcagcatcgg tcatggtgac caccaccgac tggtcggcta tccggtgggc aggcgcgccg acatagccgt cgacggcgat cacctccgcg cccaagccct gcaacgcgat cgccagctcc cggctgggtt cggccgaacc cagcaacatc acccgccgaa cgtcttgggg agctgctggg cgctcatgcc taacggtggg ttcgtgctgt tcttccgtcc agccgtcgat caccatcgaa ccaggaccct gggtctgagc ttccagggta cccatatgat ggtgatggtg atgagccatg gtatnnctcc ttc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVIDGWTEE QHEPTVRHER PAAPQDVRRV MLLGSAEPSR ELAIALQGLG AEVIAVDGYV GAPAHRIADQ SVVVTMTDAE ELTAVIRRLQ PDFLVTVTAA VSVDALDAVE QADGECTELV PNARAVRCTA DREGLRRLAA DQLGLPTAPF WFVGSLGELQ AVAVHAGFPL LVSPVAGVAG QGSSVVAGPN EVEPAWQRAA GHQVQPQTGG VSPRVCAESV VEIEFLVTMI VVCSQGPNGP LIEFCAPIGH RDADAGELES WQPQKLSTAA LDAAKSIAAR IVKALGGRGV FGVELMINGD EVYFADVTVC PAGSAWVTVR SQRLSVFELQ ARAILGLAVD TLMISPGAAR VINPDHTAGR AAVGAAPPAD ALTGALGVPE SDVVIFGRGL GVALATAPEV AIARERAREV ASRLNVPDSR E
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgatcgac ggctggacgg aagaacagca cgaacccacc gttaggcatg agcgcccagc agctccccaa gacgttcggc gggtgatgtt gctgggttcg gccgaaccca gccgggagct ggcgatcgcg ttgcagggct tgggcgcgga ggtgatcgcc gtcgacggct atgtcggcgc gcctgcccac cggatagccg accagtcggt ggtggtcacc atgaccgatg ctgaagagct gacggcggtg atccggcggc tgcaaccgga tttcttggtg acggtcaccg ccgcggtgtc tgtggatgct ctcgatgccg tcgagcaagc cgacggcgag tgcactgagc tggtgccgaa cgcccgtgcc gtccggtgca cggccgaccg ggagggcctg cgccggctgg ccgccgatca gctcggcctg cccacagccc cgttctggtt cgtcggatcc cttggcgaac ttcaagcggt ggccgtccat gctgggtttc cgttgctggt gagcccggtg gcaggggtgg ctggccaggg tagctcggtg gtcgccgggc ccaacgaggt cgagcccgcc tggcagcgcg cggcaggcca tcaagtacag ccgcagactg ggggagtgag ccctcgggtg tgcgccgagt cggtggtcga gatcgagttt ttggtcacca tgatcgttgt gtgcagtcag ggcccgaacg ggccgctcat cgagttctgt gcacctatcg gtcatcgcga cgccgatgcc ggtgagttgg aatcctggca accgcagaag ctgagcacgg cggcgctgga cgcggccaag tcgatcgccg cgcgcatcgt caaggcgctc gggggacgcg gggttttcgg cgtcgaattg atgatcaacg gcgatgaggt gtatttcgcc gatgtcaccg tgtgtcctgc cgggagtgcc tgggtcaccg tgcgcagcca gcggctttcg gtgttcgaac tgcaggcccg ggcgatcctg ggtctggcgg tggacaccct gatgatctcg ccgggtgccg cgcgggtgat caacccggac cacacggcag gccgggcagc ggtcggcgcc gcaccacctg ccgatgcgct gaccggtgcg ctcggtgtgc cggaaagcga cgtcgtgata ttcggccgcg ggcttggggt ggcgctggcc accgcacccg aggtggcaat cgcccgcgaa cgcgcccgcg aagttgcatc tcggctaaat gtgccagact cacgcgagaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa
Details for MytuD.00774.a.A1.PS00210
PURIFICATION DATe: 3/19/2009
CONCENTRATION: 18.84mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 5
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVIDGWTEE QHEPTVRHER PAAPQDVRRV MLLGSAEPSR ELAIALQGLG AEVIAVDGYV GAPAHRIADQ SVVVTMTDAE ELTAVIRRLQ PDFLVTVTAA VSVDALDAVE QADGECTELV PNARAVRCTA DREGLRRLAA DQLGLPTAPF WFVGSLGELQ AVAVHAGFPL LVSPVAGVAG QGSSVVAGPN EVEPAWQRAA GHQVQPQTGG VSPRVCAESV VEIEFLVTMI VVCSQGPNGP LIEFCAPIGH RDADAGELES WQPQKLSTAA LDAAKSIAAR IVKALGGRGV FGVELMINGD EVYFADVTVC PAGSAWVTVR SQRLSVFELQ ARAILGLAVD TLMISPGAAR VINPDHTAGR AAVGAAPPAD ALTGALGVPE SDVVIFGRGL GVALATAPEV AIARERAREV ASRLNVPDSR E
Validated NT Sequence
ttattactcg cgtgagtctg gcacatttag ccgagatgca acttcgcggg cgcgttcgcg ggcgattgcc acctcgggtg cggtggccag cgccacccca agcccgcggc cgaatatcac gacgtcgctt tccggcacac cgagcgcacc ggtcagcgca tcggcaggtg gtgcggcgcc gaccgctgcc cggcctgccg tgtggtccgg gttgatcacc cgcgcggcac ccggcgagat catcagggtg tccaccgcca gacccaggat cgcccgggcc tgcagttcga acaccgaaag ccgctggctg cgcacggtga cccaggcact cccggcagga cacacggtga catcggcgaa atacacctca tcgccgttga tcatcaattc gacgccgaaa accccgcgtc ccccgagcgc cttgacgatg cgcgcggcga tcgacttggc cgcgtccagc gccgccgtgc tcagcttctg cggttgccag gattccaact caccggcatc ggcgtcgcga tgaccgatag gtgcacagaa ctcgatgagc ggcccgttcg ggccctgact gcacacaacg atcatggtga ccaaaaactc gatctcgacc accgactcgg cgcacacccg agggctcact cccccagtct gcggctgtac ttgatggcct gccgcgcgct gccaggcggg ctcgacctcg ttgggcccgg cgaccaccga gctaccctgg ccagccaccc ctgccaccgg gctcaccagc aacggaaacc cagcatggac ggccaccgct tgaagttcgc caagggatcc gacgaaccag aacggggctg tgggcaggcc gagctgatcg gcggccagcc ggcgcaggcc ctcccggtcg gccgtgcacc ggacggcacg ggcgttcggc accagctcag tgcactcgcc gtcggcttgc tcgacggcat cgagagcatc cacagacacc gcggcggtga ccgtcaccaa gaaatccggt tgcagccgcc ggatcaccgc cgtcagctct tcagcatcgg tcatggtgac caccaccgac tggtcggcta tccggtgggc aggcgcgccg acatagccgt cgacggcgat cacctccgcg cccaagccct gcaacgcgat cgccagctcc cggctgggtt cggccgaacc cagcaacatc acccgccgaa cgtcttgggg agctgctggg cgctcatgcc taacggtggg ttcgtgctgt tcttccgtcc agccgtcgat caccatcgaa ccaggaccct gggtctgagc ttccagggta cccatatgat ggtgatggtg atgagccatg gtatnnctcc ttc
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVIDGWTEE QHEPTVRHER PAAPQDVRRV MLLGSAEPSR ELAIALQGLG AEVIAVDGYV GAPAHRIADQ SVVVTMTDAE ELTAVIRRLQ PDFLVTVTAA VSVDALDAVE QADGECTELV PNARAVRCTA DREGLRRLAA DQLGLPTAPF WFVGSLGELQ AVAVHAGFPL LVSPVAGVAG QGSSVVAGPN EVEPAWQRAA GHQVQPQTGG VSPRVCAESV VEIEFLVTMI VVCSQGPNGP LIEFCAPIGH RDADAGELES WQPQKLSTAA LDAAKSIAAR IVKALGGRGV FGVELMINGD EVYFADVTVC PAGSAWVTVR SQRLSVFELQ ARAILGLAVD TLMISPGAAR VINPDHTAGR AAVGAAPPAD ALTGALGVPE SDVVIFGRGL GVALATAPEV AIARERAREV ASRLNVPDSR E
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTGATCGAC GGCTGGACGG AAGAACAGCA CGAACCCACC GTTAGGCATG AGCGCCCAGC AGCTCCCCAA GACGTTCGGC GGGTGATGTT GCTGGGTTCG GCCGAACCCA GCCGGGAGCT GGCGATCGCG TTGCAGGGCT TGGGCGCGGA GGTGATCGCC GTCGACGGCT ATGTCGGCGC GCCTGCCCAC CGGATAGCCG ACCAGTCGGT GGTGGTCACC ATGACCGATG CTGAAGAGCT GACGGCGGTG ATCCGGCGGC TGCAACCGGA TTTCTTGGTG ACGGTCACCG CCGCGGTGTC TGTGGATGCT CTCGATGCCG TCGAGCAAGC CGACGGCGAG TGCACTGAGC TGGTGCCGAA CGCCCGTGCC GTCCGGTGCA CGGCCGACCG GGAGGGCCTG CGCCGGCTGG CCGCCGATCA GCTCGGCCTG CCCACAGCCC CGTTCTGGTT CGTCGGATCC CTTGGCGAAC TTCAAGCGGT GGCCGTCCAT GCTGGGTTTC CGTTGCTGGT GAGCCCGGTG GCAGGGGTGG CTGGCCAGGG TAGCTCGGTG GTCGCCGGGC CCAACGAGGT CGAGCCCGCC TGGCAGCGCG CGGCAGGCCA TCAAGTACAG CCGCAGACTG GGGGAGTGAG CCCTCGGGTG TGCGCCGAGT CGGTGGTCGA GATCGAGTTT TTGGTCACCA TGATCGTTGT GTGCAGTCAG GGCCCGAACG GGCCGCTCAT CGAGTTCTGT GCACCTATCG GTCATCGCGA CGCCGATGCC GGTGAGTTGG AATCCTGGCA ACCGCAGAAG CTGAGCACGG CGGCGCTGGA CGCGGCCAAG TCGATCGCCG CGCGCATCGT CAAGGCGCTC GGGGGACGCG GGGTTTTCGG CGTCGAATTG ATGATCAACG GCGATGAGGT GTATTTCGCC GATGTCACCG TGTGTCCTGC CGGGAGTGCC TGGGTCACCG TGCGCAGCCA GCGGCTTTCG GTGTTCGAAC TGCAGGCCCG GGCGATCCTG GGTCTGGCGG TGGACACCCT GATGATCTCG CCGGGTGCCG CGCGGGTGAT CAACCCGGAC CACACGGCAG GCCGGGCAGC GGTCGGCGCC GCACCACCTG CCGATGCGCT GACCGGTGCG CTCGGTGTGC CGGAAAGCGA CGTCGTGATA TTCGGCCGCG GGCTTGGGGT GGCGCTGGCC ACCGCACCCG AGGTGGCAAT CGCCCGCGAA CGCGCCCGCG AAGTTGCATC TCGGCTAAAT GTGCCAGACT CACGCGAGAA ACAGCACGAA CAAGTTCTGC AGCCAAGCTT CTCGAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA