SSGCID: Target Page

MymaA.00774.a

Formate-dependent phosphoribosylglycinamide formyltransferase PurT

CENTER ID:	MymaA.00774.a
ORGANISM:	Mycobacterium marinum ATCC BAA-535 / M
ASSOCIATED DISEASE:	Cutaneous ulcerations, deep abscesses
CURRENT STATUS:	crystallized
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MymaA.00774.a.A1.GE30070	Full length( MymaA.00774.a )		1	445

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MymaA.00774.a.A1.PS00834	Full length( MymaA.00774.a )		1	445

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|216594.6.peg.736
RefSeq:	YP_001849000.1
UniProt:	B2HPW8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTDGLTEGHD EAIGKHQRPA TEGANAPEGD KTTTIAVPPV GTPRRAEADV HVRPRVVLLG GGEQGRELTI ALQGLGAEVI AVDTDADAPA HRVADQALVV AMTDADELKV VFTRLRPDFV VPLTDAVSVA ALDALADRAP SEGGSIGLVP SARCVRLAGD REALRKLAAD QLGLPTAPFW FVGSLGELEA VAAHVGYPLL VKPVAGADVP RRSLVRGPDE VEAAWARAVA GGPAGAPPRV WAETQVEVEF LVTLIAVCSQ GPQGPRIDFC APIGHGGDGG DQESWQPQKV SVAALDAAKS IAARIVKALG GCGVFGVELM VNGDEVYFAD VQPFPRDTAW VTLRSQRLSV FELQARAILG FAVDTLMVSP GAARLIGAAT VGPVPGADAL AAALAVPESD VRVFGRPRAG SSVLGVALAT APDVAVARDR AREVVDRLNV RDSRG

NT Sequence

GTGACTGACG GCTTGACCGA AGGACACGAC GAAGCCATCG GCAAGCATCA ACGCCCGGCG ACCGAAGGCG CCAACGCTCC CGAAGGCGAC AAGACGACCA CCATCGCGGT TCCGCCCGTT GGGACACCGC GGCGAGCCGA AGCCGACGTC CACGTCAGGC CTCGGGTCGT GCTCTTGGGA GGTGGCGAGC AGGGCCGCGA GCTGACGATC GCGCTGCAGG GCCTTGGAGC CGAGGTGATC GCCGTTGACA CAGACGCCGA TGCGCCCGCG CACCGCGTCG CCGATCAGGC GCTGGTGGTG GCGATGACCG ATGCCGACGA GCTGAAGGTG GTCTTCACGC GGCTACGGCC GGATTTTGTG GTGCCGCTTA CCGACGCGGT GAGCGTCGCT GCCCTCGACG CCCTGGCTGA CCGCGCGCCC TCCGAAGGCG GTTCGATCGG GCTGGTGCCC AGCGCCCGGT GTGTCCGGCT TGCTGGCGAT CGCGAGGCCC TGCGCAAGCT GGCCGCCGAC CAGCTCGGAC TGCCCACCGC GCCATTCTGG TTCGTCGGAT CCCTTGGCGA GCTCGAGGCG GTCGCCGCAC ACGTGGGCTA CCCGCTGCTC GTCAAGCCGG TAGCCGGCGC CGACGTTCCG CGGCGGTCTT TGGTCCGTGG GCCCGACGAA GTGGAGGCCG CCTGGGCGCG GGCTGTCGCG GGCGGGCCTG CGGGAGCCCC ACCCCGGGTG TGGGCCGAGA CCCAGGTTGA GGTCGAGTTT TTGGTCACTT TGATCGCGGT GTGCAGCCAG GGGCCGCAAG GTCCGCGCAT CGACTTCTGC GCACCCATCG GCCACGGTGG CGACGGTGGT GACCAGGAAT CCTGGCAGCC GCAGAAGGTG AGTGTGGCCG CACTCGATGC GGCGAAGTCG ATCGCCGCCC GCATCGTCAA GGCGCTGGGG GGATGTGGGG TTTTCGGTGT CGAGTTGATG GTCAACGGCG ACGAGGTCTA CTTTGCCGAC GTCCAACCAT TCCCGAGGGA TACCGCGTGG GTTACCCTGC GTAGCCAGCG CCTTTCTGTC TTCGAGTTGC AAGCAAGGGC GATCTTGGGC TTTGCGGTGG ACACGTTGAT GGTGTCACCC GGTGCCGCTC GGTTGATCGG TGCGGCGACC GTTGGTCCGG TGCCGGGCGC CGATGCGCTG GCCGCGGCAC TCGCCGTGCC CGAAAGCGAT GTCCGGGTGT TCGGGCGTCC GCGTGCCGGT TCCTCCGTCC TCGGCGTGGC ACTGGCTACC GCACCCGATG TCGCTGTCGC TCGCGATCGC GCCCGCGAGG TCGTGGACCG GCTGAATGTG CGAGACTCAC GCGGG

Details for MymaA.00774.a.A1.GE30070

HARVESTED ON:	8/10/2010
SEQUENCED ON:	8/16/2010
EXPECTED MW:	48kDa
OBSERVED MW:	49kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	99
PERCENT COVERAGE:	95

Validated AA Sequence

MVTDGLTEGH DEAIGKHQRP ATEGANAPEG DKTTTIAVPP VGTPRRAEAD VHVRPRVVLL GGGEQGRELT IALQGLGAEV IAVDTDADAP AHRVADQALV VAMTDADELK VVFTRLRPDF VVPLTDAVSV AALDALADRA PSEGGSIGLV PSARCVRLAG DREALRKLAA DQLGLPTAPF WFVGSLGELE AVAAHVGYPL LVKPVAGADV PRRSLVRGPD EVEAAWARAV AGGPAGAPPR VWAETQVEVE FLVTLIAVCS QGPQGPRIDF CAPIGHGGDG GDQESWQPQK VSVAALDAAK SIAARIVKAL GGCGVFGVEL MVNGDEVYFA DVQPFPRDTA WVTLRSQRLS VFELQARAIL GFAVDTLMVS PGAARLIGAA TVGPVPXXDA LAAALAVPES DVRVFGRPRA GSSVLGVALA TAPDVAVARD RAREVVDRLN VRDS

Validated NT Sequence

cgtgagtctc gcacattcag ccggtccacg acctcgcggg cgcgatcgcg agcgacagcg acatcgggtg cggtagccag tgccacgccg aggacggagg aaccggcacg cggacgcccg aacacccgga catcgctttc gggcacggcg agtgccgcgg ccagcgcatc nnnnnncggc accggaccaa cggtcgccgc accgatcaac cgagcggcac cgggtgacac catcaacgtg tccaccgcaa agcccaagat cgcccttgct tgcaactcga agacagaaag gcgctggcta cgcagggtaa cccacgcggt atccctcggg aatggttgga cgtcggcaaa gtagacctcg tcgccgttga ccatcaactc gacaccgaaa accccacatc cccccagcgc cttgacgatg cgggcggcga tcgacttcgc cgcatcgagt gcggccacac tcaccttctg cggctgccag gattcctggt caccaccgtc gccaccgtgg ccgatgggtg cgcagaagtc gatgcgcgga ccttgcggcc cctggctgca caccgcgatc aaagtgacca aaaactcgac ctcaacctgg gtctcggccc acacccgggg tggggctccc gcaggcccgc ccgcgacagc ccgcgcccag gcggcctcca cttcgtcggg cccacggacc aaagaccgcc gcggaacgtc ggcgccggct accggcttga cgagcagcgg gtagcccacg tgtgcggcga ccgcctcgag ctcgccaagg gatccgacga accagaatgg cgcggtgggc agtccgagct ggtcggcggc cagcttgcgc agggcctcgc gatcgccagc aagccggaca caccgggcgc tgggcaccag cccgatcgaa ccgccttcgg agggcgcgcg gtcagccagg gcgtcgaggg cagcgacgct caccgcgtcg gtaagcggca ccacaaaatc cggccgtagc cgcgtgaaga ccaccttcag ctcgtcggca tcggtcatcg ccaccaccag cgcctgatcg gcgacgcggt gcgcgggcgc atcggcgtct gtgtcaacgg cgatcacctc ggctccaagg ccctgcagcg cgatcgtcag ctcgcggccc tgctcgccac ctcccaagag cacgacccga ggcctgacgt ggacgtcggc ttcggctcgc cgcggtgtcc caacgggcgg aaccgcgatg gtggtcgtct tgtcgccttc gggagcgttg gcgccttcgg tcgccgggcg ttgatgcttg ccgatggctt cgtcgtgtcc ttcggtcaag ccgtcagtca ccatcgaacc aggaccctgg gtctgagctt ccagg

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMVTDGLTEG HDEAIGKHQR PATEGANAPE GDKTTTIAVP PVGTPRRAEA DVHVRPRVVL LGGGEQGREL TIALQGLGAE VIAVDTDADA PAHRVADQAL VVAMTDADEL KVVFTRLRPD FVVPLTDAVS VAALDALADR APSEGGSIGL VPSARCVRLA GDREALRKLA ADQLGLPTAP FWFVGSLGEL EAVAAHVGYP LLVKPVAGAD VPRRSLVRGP DEVEAAWARA VAGGPAGAPP RVWAETQVEV EFLVTLIAVC SQGPQGPRID FCAPIGHGGD GGDQESWQPQ KVSVAALDAA KSIAARIVKA LGGCGVFGVE LMVNGDEVYF ADVQPFPRDT AWVTLRSQRL SVFELQARAI LGFAVDTLMV SPGAARLIGA ATVGPVPGAD ALAAALAVPE SDVRVFGRPR AGSSVLGVAL ATAPDVAVAR DRAREVVDRL NVRDSR

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgactgac ggcttgaccg aaggacacga cgaagccatc ggcaagcatc aacgcccggc gaccgaaggc gccaacgctc ccgaaggcga caagacgacc accatcgcgg ttccgcccgt tgggacaccg cggcgagccg aagccgacgt ccacgtcagg cctcgggtcg tgctcttggg aggtggcgag cagggccgcg agctgacgat cgcgctgcag ggccttggag ccgaggtgat cgccgttgac acagacgccg atgcgcccgc gcaccgcgtc gccgatcagg cgctggtggt ggcgatgacc gatgccgacg agctgaaggt ggtcttcacg cggctacggc cggattttgt ggtgccgctt accgacgcgg tgagcgtcgc tgccctcgac gccctggctg accgcgcgcc ctccgaaggc ggttcgatcg ggctggtgcc cagcgcccgg tgtgtccggc ttgctggcga tcgcgaggcc ctgcgcaagc tggccgccga ccagctcgga ctgcccaccg cgccattctg gttcgtcgga tcccttggcg agctcgaggc ggtcgccgca cacgtgggct acccgctgct cgtcaagccg gtagccggcg ccgacgttcc gcggcggtct ttggtccgtg ggcccgacga agtggaggcc gcctgggcgc gggctgtcgc gggcgggcct gcgggagccc caccccgggt gtgggccgag acccaggttg aggtcgagtt tttggtcact ttgatcgcgg tgtgcagcca ggggccgcaa ggtccgcgca tcgacttctg cgcacccatc ggccacggtg gcgacggtgg tgaccaggaa tcctggcagc cgcagaaggt gagtgtggcc gcactcgatg cggcgaagtc gatcgccgcc cgcatcgtca aggcgctggg gggatgtggg gttttcggtg tcgagttgat ggtcaacggc gacgaggtct actttgccga cgtccaacca ttcccgaggg ataccgcgtg ggttaccctg cgtagccagc gcctttctgt cttcgagttg caagcaaggg cgatcttggg ctttgcggtg gacacgttga tggtgtcacc cggtgccgct cggttgatcg gtgcggcgac cgttggtccg gtgccgggcg ccgatgcgct ggccgcggca ctcgccgtgc ccgaaagcga tgtccgggtg ttcgggcgtc cgcgtgccgg ttcctccgtc ctcggcgtgg cactggctac cgcacccgat gtcgctgtcg ctcgcgatcg cgcccgcgag gtcgtggacc ggctgaatgt gcgagactca cgcaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa

Details for MymaA.00774.a.A1.PS00834

PURIFICATION DATe:	11/1/2010
CONCENTRATION:	36.11mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	High Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	4
VIAL VOLUME:	200µl
PERCENT IDENTITY:	99
PERCENT COVERAGE:	95

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTGACTGAC GGCTTGACCG AAGGACACGA CGAAGCCATC GGCAAGCATC AACGCCCGGC GACCGAAGGC GCCAACGCTC CCGAAGGCGA CAAGACGACC ACCATCGCGG TTCCGCCCGT TGGGACACCG CGGCGAGCCG AAGCCGACGT CCACGTCAGG CCTCGGGTCG TGCTCTTGGG AGGTGGCGAG CAGGGCCGCG AGCTGACGAT CGCGCTGCAG GGCCTTGGAG CCGAGGTGAT CGCCGTTGAC ACAGACGCCG ATGCGCCCGC GCACCGCGTC GCCGATCAGG CGCTGGTGGT GGCGATGACC GATGCCGACG AGCTGAAGGT GGTCTTCACG CGGCTACGGC CGGATTTTGT GGTGCCGCTT ACCGACGCGG TGAGCGTCGC TGCCCTCGAC GCCCTGGCTG ACCGCGCGCC CTCCGAAGGC GGTTCGATCG GGCTGGTGCC CAGCGCCCGG TGTGTCCGGC TTGCTGGCGA TCGCGAGGCC CTGCGCAAGC TGGCCGCCGA CCAGCTCGGA CTGCCCACCG CGCCATTCTG GTTCGTCGGA TCCCTTGGCG AGCTCGAGGC GGTCGCCGCA CACGTGGGCT ACCCGCTGCT CGTCAAGCCG GTAGCCGGCG CCGACGTTCC GCGGCGGTCT TTGGTCCGTG GGCCCGACGA AGTGGAGGCC GCCTGGGCGC GGGCTGTCGC GGGCGGGCCT GCGGGAGCCC CACCCCGGGT GTGGGCCGAG ACCCAGGTTG AGGTCGAGTT TTTGGTCACT TTGATCGCGG TGTGCAGCCA GGGGCCGCAA GGTCCGCGCA TCGACTTCTG CGCACCCATC GGCCACGGTG GCGACGGTGG TGACCAGGAA TCCTGGCAGC CGCAGAAGGT GAGTGTGGCC GCACTCGATG CGGCGAAGTC GATCGCCGCC CGCATCGTCA AGGCGCTGGG GGGATGTGGG GTTTTCGGTG TCGAGTTGAT GGTCAACGGC GACGAGGTCT ACTTTGCCGA CGTCCAACCA TTCCCGAGGG ATACCGCGTG GGTTACCCTG CGTAGCCAGC GCCTTTCTGT CTTCGAGTTG CAAGCAAGGG CGATCTTGGG CTTTGCGGTG GACACGTTGA TGGTGTCACC CGGTGCCGCT CGGTTGATCG GTGCGGCGAC CGTTGGTCCG GTGCCGGGCG CCGATGCGCT GGCCGCGGCA CTCGCCGTGC CCGAAAGCGA TGTCCGGGTG TTCGGGCGTC CGCGTGCCGG TTCCTCCGTC CTCGGCGTGG CACTGGCTAC CGCACCCGAT GTCGCTGTCG CTCGCGATCG CGCCCGCGAG GTCGTGGACC GGCTGAATGT GCGAGACTCA CGCAAACAGC ACGAACAAGT TCTGCAGCCA AGCTTCTCGA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAA