MytuD.00690.a

PROBABLE UNIDENTIFIED ANTIBIOTIC-TRANSPORT ATP-BINDING PROTEIN ABC TRANSPORTER

CENTER ID: MytuD.00690.a
ORGANISM: Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE: Tuberculosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MytuD.00690.a.B1.GE27588 Full length( MytuD.00690.a ) 1 313
MytuD.00690.a.B1.GE27167 Full length( MytuD.00690.a ) 1 313
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|83332.12.peg.1629
RefSeq: NP_215974.1
TubercuList: Rv1458c
MTB Network Portal: Rv1458c
UniProt: O53149

Orthologues for Rv1458c

Species % Identity % Coverage Clones Proteins Structures
marinum 91 97 1 0 0
avium 91 96 0 0 0
paratuberculosis 90 98 0 0 0
leprae 88 98 1 0 0
smegmatis 83 100 1 0 0
abscessus 80 91 1 0 0
smegmatis 47 69 1 0 0
marinum 34 90 1 0 0
ulcerans 34 90 1 0 0
abscessus 32 70 0 0 0
abscessus 31 95 1 0 0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNRAPDTPEV VLRLRGVCKR YGSITAVSNL DLDVHDAEVM ALLGPNGAGK TTTVEMCEGF VRPDAGSIEV LGLDPITDNA RLRARIGVML QGGGGYPAAR AGEMLDLVAS YAANPLDPHW LLDTLGLTEA ARTTYRRLSG GQQQRLALAC ALVGRPQLVF LDEPTAGMDA HARVLVWELI DALRRDGVTV VLTTHHLKEA EELADRLVII DHGVTVAAGT PAELMRSGAK DQLRFTAPPR LDLSLLASAL PEGYQATELT PGEYLVEGPV DPQVLATVTA WCAQIDVLAT DMRVEQRSLE DVFLDLTGRK LRQ
NT Sequence
GTGAACCGCG CCCCAGACAC CCCCGAGGTG GTACTGCGGC TTCGCGGGGT ATGTAAGCGA TACGGGTCAA TAACCGCCGT TTCCAACCTC GATCTCGACG TGCATGACGC CGAAGTGATG GCGCTGCTGG GCCCCAACGG CGCGGGCAAG ACCACGACCG TCGAGATGTG CGAGGGCTTC GTCCGCCCGG ACGCCGGCAG CATTGAGGTA CTTGGACTGG ACCCGATCAC CGACAACGCA CGCCTGCGCG CACGCATCGG CGTGATGTTG CAGGGTGGCG GCGGCTACCC GGCGGCACGC GCCGGCGAAA TGCTGGATCT GGTCGCGTCC TACGCCGCCA ACCCGCTGGA CCCGCACTGG CTACTGGACA CCTTGGGTCT CACCGAGGCC GCCCGCACCA CCTATCGACG GCTCTCCGGC GGGCAGCAGC AACGACTCGC GCTGGCCTGC GCGCTGGTCG GGCGTCCCCA ACTGGTGTTC CTCGACGAGC CCACCGCGGG CATGGATGCC CATGCCCGGG TACTGGTGTG GGAGTTGATC GACGCGCTGC GCCGCGATGG CGTGACCGTG GTGCTGACCA CGCATCATCT CAAGGAGGCC GAGGAACTCG CCGATCGGTT GGTGATCATC GACCACGGGG TGACGGTGGC CGCAGGCACA CCGGCAGAAC TGATGCGCAG CGGCGCCAAA GACCAGTTGC GGTTCACCGC GCCGCCGCGG CTCGACCTGT CGCTGCTGGC CTCGGCACTG CCCGAGGGCT ACCAGGCCAC CGAGCTGACC CCGGGCGAGT ACCTGGTCGA AGGTCCGGTT GACCCGCAGG TGCTGGCGAC GGTCACCGCG TGGTGCGCAC AAATCGACGT GCTAGCCACC GACATGCGGG TCGAGCAACG CAGCCTCGAA GACGTGTTCC TGGATCTCAC CGGCAGGAAG TTGCGGCAG
Details for MytuD.00690.a.B1.GE27588
HARVESTED ON: 8/17/2009
SEQUENCED ON: 9/29/2009
EXPECTED MW: 34kDa
OBSERVED MW: 35kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMV NRAPDTPEVV LRLRGVCKRY GSITAVSNLD LDVHDAEVMA LLGPNGAGKT TTVEMCEGFV RPDAGSIEVL GLDPITDNAR LRARIGVMLQ GGGGYPAARA GEMLDLVASY AANPLDPHWL LDTLGLTEAA RTTYRRLSGG QQQRLALACA LVGRPQLVFL DEPTAGMDAH ARVLVWELID ALRRDGVTVV LTTHHLKEAE ELADRLVIID HGVTVAAGTP AELMRSGAKD QLRFTAPPRL DLSLLASALP EGYQATELTP GEYLVEGPVD PQVLATVTAW CAQIDVLATD MRVEQRSLED VFLDLTGRKL RQ
Validated NT Sequence
atggctcacc accaccacca ccatatggtg aaccgcgccc cagacacccc cgaggtggta ctgcggcttc gcggggtatg taagcgatac gggtcaataa ccgccgtttc caacctcgat ctcgacgtgc atgacgccga agtgatggcg ctgctgggcc ccaacggcgc gggcaagacc acgaccgtcg agatgtgcga gggcttcgtc cgcccggacg ccggcagcat tgaggtactt ggactggacc cgatcaccga caacgcacgc ctgcgcgcac gcatcggcgt gatgttgcag ggtggcggcg gctacccggc ggcacgcgcc ggcgaaatgc tggatctggt cgcgtcctac gccgccaacc cgctggaccc gcactggcta ctggacacct tgggtctcac cgaggccgcc cgcaccacct atcgacggct ctccggcggg cagcagcaac gactcgcgct ggcctgcgcg ctggtcgggc gtccccaact ggtgttcctc gacgagccca ccgcgggcat ggatgcccat gcccgggtac tggtgtggga gttgatcgac gcgctgcgcc gcgatggcgt gaccgtggtg ctgaccacgc atcatctcaa ggaggccgag gaactcgccg atcggttggt gatcatcgac cacggggtga cggtggccgc aggcacaccg gcagaactga tgcgcagcgg cgccaaagac cagttgcggt tcaccgcgcc gccgcggctc gacctgtcgc tgctggcctc ggcactgccc gagggctacc aggccaccga gctgaccccg ggcgagtacc tggtcgaagg tccggttgac ccgcaggtgc tggcgacggt caccgcgtgg tgcgcacaaa tcgacgtgct agccaccgac atgcgggtcg agcaacgcag cctcgaagac gtgttcctgg atctcaccgg caggaagttg cggcagtaag tgagta
Expected Protein Sequence
MAHHHHHHMV NRAPDTPEVV LRLRGVCKRY GSITAVSNLD LDVHDAEVMA LLGPNGAGKT TTVEMCEGFV RPDAGSIEVL GLDPITDNAR LRARIGVMLQ GGGGYPAARA GEMLDLVASY AANPLDPHWL LDTLGLTEAA RTTYRRLSGG QQQRLALACA LVGRPQLVFL DEPTAGMDAH ARVLVWELID ALRRDGVTVV LTTHHLKEAE ELADRLVIID HGVTVAAGTP AELMRSGAKD QLRFTAPPRL DLSLLASALP EGYQATELTP GEYLVEGPVD PQVLATVTAW CAQIDVLATD MRVEQRSLED VFLDLTGRKL RQ
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggtga accgcgcccc agacaccccc gaggtggtac tgcggcttcg cggggtatgt aagcgatacg ggtcaataac cgccgtttcc aacctcgatc tcgacgtgca tgacgccgaa gtgatggcgc tgctgggccc caacggcgcg ggcaagacca cgaccgtcga gatgtgcgag ggcttcgtcc gcccggacgc cggcagcatt gaggtacttg gactggaccc gatcaccgac aacgcacgcc tgcgcgcacg catcggcgtg atgttgcagg gtggcggcgg ctacccggcg gcacgcgccg gcgaaatgct ggatctggtc gcgtcctacg ccgccaaccc gctggacccg cactggctac tggacacctt gggtctcacc gaggccgccc gcaccaccta tcgacggctc tccggcgggc agcagcaacg actcgcgctg gcctgcgcgc tggtcgggcg tccccaactg gtgttcctcg acgagcccac cgcgggcatg gatgcccatg cccgggtact ggtgtgggag ttgatcgacg cgctgcgccg cgatggcgtg accgtggtgc tgaccacgca tcatctcaag gaggccgagg aactcgccga tcggttggtg atcatcgacc acggggtgac ggtggccgca ggcacaccgg cagaactgat gcgcagcggc gccaaagacc agttgcggtt caccgcgccg ccgcggctcg acctgtcgct gctggcctcg gcactgcccg agggctacca ggccaccgag ctgaccccgg gcgagtacct ggtcgaaggt ccggttgacc cgcaggtgct ggcgacggtc accgcgtggt gcgcacaaat cgacgtgcta gccaccgaca tgcgggtcga gcaacgcagc ctcgaagacg tgttcctgga tctcaccggc aggaagttgc ggcagtgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat
Details for MytuD.00690.a.B1.GE27167
HARVESTED ON: 7/20/2009
SEQUENCED ON: 7/28/2009
EXPECTED MW: 34kDa
OBSERVED MW: 35kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMV NRAPDTPEVV LRLRGVCKRY GSITAVSNLD LDVHDAEVMA LLGPNGAGKT TTVEMCEGFV RPDAGSIEVL GLDPITDNAR LRARIGVMLQ GGGGYPAARA GEMLDLVASY AANPLDPHWL LDTLGLTEAA RTTYRRLSGG QQQRLALACA LVGRPQLVFL DEPTAGMDAH ARVLVWELID ALRRDGVTVV LTTHHLKEAE ELADRLVIID HGVTVAAGTP AELMRSGAKD QLRFTAPPRL DLSLLASALP EGYQATELTP GEYLVEGPVD PQVLATVTAW CAQIDVLATD MRVEQRSLED VFLDLTGRKL RQ
Validated NT Sequence
atggctcacc accaccacca ccatatggtg aaccgcgccc cagacacccc cgaggtggta ctgcggcttc gcggggtatg taagcgatac gggtcaataa ccgccgtttc caacctcgat ctcgacgtgc atgacgccga agtgatggcg ctgctgggcc ccaacggcgc gggcaagacc acgaccgtcg agatgtgcga gggcttcgtc cgcccggacg ccggcagcat tgaggtactt ggactggacc cgatcaccga caacgcacgc ctgcgcgcac gcatcggcgt gatgttgcag ggtggcggcg gctacccggc ggcacgcgcc ggcgaaatgc tggatctggt cgcgtcctac gccgccaacc cgctggaccc gcactggcta ctggacacct tgggtctcac cgaggccgcc cgcaccacct atcgacggct ctccggcggg cagcagcaac gactcgcgct ggcctgcgcg ctggtcgggc gtccccaact ggtgttcctc gacgagccca ccgcgggcat ggatgcccat gcccgggtac tggtgtggga gttgatcgac gcgctgcgcc gcgatggcgt gaccgtggtg ctgaccacgc atcatctcaa ggaggccgag gaactcgccg atcggttggt gatcatcgac cacggggtga cggtggccgc aggcacaccg gcagaactga tgcgcagcgg cgccaaagac cagttgcggt tcaccgcgcc gccgcggctc gacctgtcgc tgctggcctc ggcactgccc gagggctacc aggccaccga gctgaccccg ggcgagtacc tggtcgaagg tccggttgac ccgcaggtgc tggcgacggt caccgcgtgg tgcgcacaaa tcgacgtgct agccaccgac atgcgggtcg agcaacgcag cctcgaagac gtgttcctgg atctcaccgg caggaagttg cggcagtaag tgagta
Expected Protein Sequence
MAHHHHHHMV NRAPDTPEVV LRLRGVCKRY GSITAVSNLD LDVHDAEVMA LLGPNGAGKT TTVEMCEGFV RPDAGSIEVL GLDPITDNAR LRARIGVMLQ GGGGYPAARA GEMLDLVASY AANPLDPHWL LDTLGLTEAA RTTYRRLSGG QQQRLALACA LVGRPQLVFL DEPTAGMDAH ARVLVWELID ALRRDGVTVV LTTHHLKEAE ELADRLVIID HGVTVAAGTP AELMRSGAKD QLRFTAPPRL DLSLLASALP EGYQATELTP GEYLVEGPVD PQVLATVTAW CAQIDVLATD MRVEQRSLED VFLDLTGRKL RQ
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggtga accgcgcccc agacaccccc gaggtggtac tgcggcttcg cggggtatgt aagcgatacg ggtcaataac cgccgtttcc aacctcgatc tcgacgtgca tgacgccgaa gtgatggcgc tgctgggccc caacggcgcg ggcaagacca cgaccgtcga gatgtgcgag ggcttcgtcc gcccggacgc cggcagcatt gaggtacttg gactggaccc gatcaccgac aacgcacgcc tgcgcgcacg catcggcgtg atgttgcagg gtggcggcgg ctacccggcg gcacgcgccg gcgaaatgct ggatctggtc gcgtcctacg ccgccaaccc gctggacccg cactggctac tggacacctt gggtctcacc gaggccgccc gcaccaccta tcgacggctc tccggcgggc agcagcaacg actcgcgctg gcctgcgcgc tggtcgggcg tccccaactg gtgttcctcg acgagcccac cgcgggcatg gatgcccatg cccgggtact ggtgtgggag ttgatcgacg cgctgcgccg cgatggcgtg accgtggtgc tgaccacgca tcatctcaag gaggccgagg aactcgccga tcggttggtg atcatcgacc acggggtgac ggtggccgca ggcacaccgg cagaactgat gcgcagcggc gccaaagacc agttgcggtt caccgcgccg ccgcggctcg acctgtcgct gctggcctcg gcactgcccg agggctacca ggccaccgag ctgaccccgg gcgagtacct ggtcgaaggt ccggttgacc cgcaggtgct ggcgacggtc accgcgtggt gcgcacaaat cgacgtgcta gccaccgaca tgcgggtcga gcaacgcagc ctcgaagacg tgttcctgga tctcaccggc aggaagttgc ggcagtgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat