MyabA.00690.b

Putative ABC transporter, ATP-binding protein

CENTER ID: MyabA.00690.b
ORGANISM: Mycobacterium abscessus ATCC 19977 / DSM 44196
ASSOCIATED DISEASE: Skin abscess
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyabA.00690.b.A1.GE30334 Full length( MyabA.00690.b ) 1 324
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|36809.5.peg.4294
RefSeq: YP_001704942.1
UniProt: B1MIR7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MIELRSLTKV YGQTPAVDDL SVTIESGVVT GFLGPNGAGK STTLRMIVGL ARPTSGTATI GGKQYHEIDH PLREVGALLD PKQFHPNRSA RNHLRWIAAA NDISAQRVDE VLEMVGLTEV ANRHAGTFSL GMSQRLGIAV ALLGDPKVLL FDEPVNGLDP EGIHWIRTLM QSLAAQGRTV LVSSHLLSEM ANTADQLVVI GRGRLIAATS MHEFVSRAGA DVVRVRSPQI DILRSAAEES GFSVVGGERT GEPGVLEISG ALIEQVGELA ARRGVTLYEL SPQRVSLEDA YLSLTDDAVQ YRSQVNDPLA ARATGAVADA EGTH
NT Sequence
ATGATCGAAC TGCGGTCGCT GACGAAGGTC TATGGCCAGA CCCCTGCGGT CGACGACCTC TCCGTCACCA TCGAGTCGGG GGTCGTGACG GGGTTCCTGG GTCCCAACGG GGCAGGTAAG TCGACCACGT TGCGGATGAT CGTCGGGCTG GCCCGCCCGA CATCGGGGAC GGCGACGATC GGCGGCAAGC AGTACCACGA GATCGACCAC CCGCTTCGCG AGGTCGGCGC ACTGTTGGAT CCCAAGCAGT TTCATCCCAA TCGATCGGCG CGCAACCATC TACGGTGGAT CGCGGCCGCC AATGACATCT CCGCCCAGCG GGTCGACGAA GTGCTGGAGA TGGTCGGGCT GACCGAGGTG GCCAATCGCC ATGCCGGCAC CTTCTCACTC GGCATGTCGC AGCGGTTGGG CATTGCGGTC GCACTGCTTG GTGACCCTAA GGTGCTGCTG TTCGACGAGC CGGTCAACGG CCTGGATCCG GAAGGCATTC ATTGGATTCG CACCTTGATG CAGAGCCTGG CGGCACAGGG ACGCACCGTG CTGGTCTCCA GCCACCTGCT CTCTGAAATG GCCAATACCG CAGACCAATT GGTGGTCATC GGCCGTGGGC GATTGATCGC GGCGACCTCG ATGCACGAAT TTGTGAGCCG AGCGGGCGCC GATGTGGTGC GGGTGCGCAG CCCGCAAATC GATATCTTGC GTTCGGCCGC GGAGGAAAGT GGTTTCTCGG TGGTGGGTGG GGAACGCACC GGAGAGCCGG GCGTGCTCGA AATCAGCGGC GCCCTCATCG AGCAGGTCGG CGAGCTTGCC GCCCGTCGTG GCGTCACGCT GTACGAACTT TCTCCGCAAC GAGTTTCGCT GGAGGACGCG TACCTGTCGC TGACCGATGA TGCCGTGCAG TACCGCAGCC AGGTCAATGA TCCGCTTGCG GCAAGAGCGA CGGGCGCAGT GGCCGATGCG GAGGGTACGC AC
Details for MyabA.00690.b.A1.GE30334
HARVESTED ON: 9/8/2010
SEQUENCED ON: 9/17/2010
EXPECTED MW: 36kDa
OBSERVED MW: 35kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 85
PERCENT COVERAGE: 91
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMIELRSLTK VYGQTPAVDD LSVTIESGVV TGFLGPNGAG KSTTLRMIVG LARPTSGTAT IGGKQYHEID HPLREVGALL DPKQFHPNRS ARNHLRWIAA ANDISAQRVD EVLEMVGLTE VANRHAGTFS LGMSQRLGIA VALLGDPKVL LFDEPVNGLD PEGIHWIRTL MXSLAAQGRT VLVSSHLLSE MANTAXQLVV IGRGRLIXAT SMHXFVSRAG ADVVRVRNPX IXILRSAAXE SGFSXVGGEX TGEPGVLXNX XRPHXXXRXA CRPSXXXAXX XSXXSFAXRT RXCR
Validated NT Sequence
attttgttta ctttaagaag gagatatacc atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgatcg aactgcggtc gctgacgaag gtctatggcc agacccctgc ggtcgacgac ctctccgtca ccatcgagtc gggggtcgtg acggggttcc tgggtcccaa cggggcaggt aagtcgacca cgttgcggat gatcgtcggg ctggcccgcc cgacatcggg gacggcgacg atcggcggca agcagtacca cgagatcgac cacccgcttc gcgaggtcgg cgcactgttg gatcccaagc agtttcatcc caatcgatcg gcgcgcaacc atctacggtg gatcgcggcc gccaatgaca tctccgccca gcgggtcgac gaagtgctgg agatggtcgg gctgaccgag gtggccaatc gccatgccgg caccttctca ctcggcatgt cgcagcggtt gggcattgcg gtcgcactgc ttggtgaccc taaggtgctg ctgttcgacg agccggtcaa cggcctggat ccggaaggca ttcattggat tcgcaccttg atgcanagcc tggcggcaca gggacgcacc gtgctggtct ccagccacct gctctctgaa atggccaata ccgcanacca attggtggtc atcggccgtg ggcgattgat cncggcgacc tcnatgcacn aatttgtgag ccgagcgggc gccgatgtgg tgcgggtgcg caacccgcan atcnatatct tgcgttcngc cgcggangaa agtggtttct cggnggtggg tggggaangc accggagagc cnggcgtgct cnnaaatcnn nngcgccctc atcnnncang tcggcnagct tgccgcccgt cnnnnnntcn cgctnnacnn ntctccgnnn cnagtttcgc tngnaggacg cgtanntgtc gctgancgnt nnngncnngc agtaccgcan cnnnnncnnt gatccgcttn nngcgnnnnn nncnnnngnn gatgnnnnng gtnnncnnnn naannaannn nnangnnnna nntnnncanc nannnnnnnn ngnatccnnn nnntannnaa nnnnnnanc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMIELRSLTK VYGQTPAVDD LSVTIESGVV TGFLGPNGAG KSTTLRMIVG LARPTSGTAT IGGKQYHEID HPLREVGALL DPKQFHPNRS ARNHLRWIAA ANDISAQRVD EVLEMVGLTE VANRHAGTFS LGMSQRLGIA VALLGDPKVL LFDEPVNGLD PEGIHWIRTL MQSLAAQGRT VLVSSHLLSE MANTADQLVV IGRGRLIAAT SMHEFVSRAG ADVVRVRSPQ IDILRSAAEE SGFSVVGGER TGEPGVLEIS GALIEQVGEL AARRGVTLYE LSPQRVSLED AYLSLTDDAV QYRSQVNDPL AARATGAVAD AEGTH
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gatcgaactg cggtcgctga cgaaggtcta tggccagacc cctgcggtcg acgacctctc cgtcaccatc gagtcggggg tcgtgacggg gttcctgggt cccaacgggg caggtaagtc gaccacgttg cggatgatcg tcgggctggc ccgcccgaca tcggggacgg cgacgatcgg cggcaagcag taccacgaga tcgaccaccc gcttcgcgag gtcggcgcac tgttggatcc caagcagttt catcccaatc gatcggcgcg caaccatcta cggtggatcg cggccgccaa tgacatctcc gcccagcggg tcgacgaagt gctggagatg gtcgggctga ccgaggtggc caatcgccat gccggcacct tctcactcgg catgtcgcag cggttgggca ttgcggtcgc actgcttggt gaccctaagg tgctgctgtt cgacgagccg gtcaacggcc tggatccgga aggcattcat tggattcgca ccttgatgca gagcctggcg gcacagggac gcaccgtgct ggtctccagc cacctgctct ctgaaatggc caataccgca gaccaattgg tggtcatcgg ccgtgggcga ttgatcgcgg cgacctcgat gcacgaattt gtgagccgag cgggcgccga tgtggtgcgg gtgcgcagcc cgcaaatcga tatcttgcgt tcggccgcgg aggaaagtgg tttctcggtg gtgggtgggg aacgcaccgg agagccgggc gtgctcgaaa tcagcggcgc cctcatcgag caggtcggcg agcttgccgc ccgtcgtggc gtcacgctgt acgaactttc tccgcaacga gtttcgctgg aggacgcgta cctgtcgctg accgatgatg ccgtgcagta ccgcagccag gtcaatgatc cgcttgcggc aagagcgacg ggcgcagtgg ccgatgcgga gggtacgcac taaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa