MytuD.00472.a
Probable short-chain type dehydrogenase/reductase
| CENTER ID: | MytuD.00472.a |
| ORGANISM: | Mycobacterium tuberculosis H37Rv |
| ASSOCIATED DISEASE: | Tuberculosis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIC |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
Materials can be ordered from SSGCID using the button in the "order material" column.
Clicking the button will add the material to a virtual cart.
You may order multiple materials at a time at no cost to you, as this contract is funded
by NIAID. When you are ready to place your order, click the "Place Order" link which will
appear in the top right corner of the page after you place your first item in your cart.
Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MytuD.00472.a.A1.GE25846 | Full length( MytuD.00472.a ) | 1 | 256 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MytuD.00472.a.A1.PW25905 | Full length( MytuD.00472.a ) | 1 | 256 |
Structures
3GVC
DEPOSITED: 3/30/2009
DETERMINATION: XRay
CLONE: MytuD.00472.a.A1.GE25846
PROTEIN: MytuD.00472.a.A1.PW25905
Publications by SSGCID
Increasing the structural coverage of tuberculosis drug targets.
Abendroth J, Abramov A, Armour B, Barrett LK, Baugh L, Begley DW, Buchko GW, Choi R, Clifton MC, Dieterich SH, Dranow DM, Edwards TE, Fairman JW, Ferrell M, Fox D, Gardberg AS, Hewitt SN, Lorimer D, Lyons-Abbott S, Mundt E, Muruthi MM, Myers J, Myler PJ, Napuli AJ, Phan I, Sekar A, Serbzhinskiy D, Stacy R, Staker BL, Stewart LJ, Taylor BM, Thompkins K, Tran N, Van Voorhis WC, Zhang Y
Tuberculosis (Edinb) - 2014
volume 95, issue 2, pages 142-8
PMID: 25613812; PMCID: PMC4361283
PDB:
3GVC,
3GVG,
3GWC,
3H7F,
3H81,
3HE2,
3HWI,
3HWK,
3HZG,
3ICO,
3KHP,
3LLS,
3MOY,
3MPZ,
3MYB,
3NDN,
3NDO,
3NF4,
3NG3,
3NJD,
3NWO,
3O0M,
3O38,
3OC6,
3OC7,
3OEC,
3OI9,
3OKS,
3OL3,
3OL4,
3OME,
3P0T,
3P2Y,
3P4I,
3P4T,
3P5M,
3P85,
3PE8,
3PGX,
3PK0,
3PPI,
3PXX,
3PZY,
3Q1T,
3Q8N,
3QBP,
3QDF,
3QH4,
3QHA,
3QIV,
3QK8,
3QKA,
3QLJ,
3QMJ,
3QRE,
3QUA,
3QUV,
3QXI,
3QXZ,
3QYR,
3R0O,
3R1I,
3R1J,
3R20,
3R2N,
3R4T,
3R6H,
3R6O,
3R7K,
3R8C,
3R9P,
3R9Q,
3R9R,
3R9S,
3R9T,
3RD5,
3RD7,
3RD8,
3RFQ,
3RIH,
3RR2,
3RR6,
3RRP,
3RRV,
3RSI,
3RV2,
3S55,
3S82,
3SBX,
3SF6,
3SLL,
3SVK,
3SVT,
3SWO,
3SWT,
3SWX,
3SX2,
3T3W,
3T7C,
3TAV,
3TCR,
3TDE,
3TJR,
3TL3,
3TLF,
3TRR,
3TSC,
3TX2,
3TZQ,
3TZU,
3U0A,
3U0B,
3UCX,
3UVE,
4DI1,
4DIE,
4DQ8,
4DXL,
4ED4,
4EGE,
4EGF,
4EMD,
4EO9,
4EYE,
4F3W,
4F47,
4FFC,
4GK6,
4HDT,
4HR3,
4I1Y,
4IJN,
4IV6,
4IZ9,
4J5I,
4KAM,
4LGV,
4O2D,
5EJ2
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_129138 |
| PATRIC ID: | fig|83332.12.peg.2169 |
| RefSeq: | NP_216457.1 |
| TubercuList: | Rv1941 |
| MTB Network Portal: | Rv1941 |
| UniProt: | I6XZC4 |
Orthologues for Rv1941
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNHPDLAGKV AIVTGAGAGI GLAVARRLAD EGCHVLCADI DGDAADAAAT KIGCGAAACR VDVSDEQQII AMVDACVAAF GGVDKLVANA GVVHLASLID TTVEDFDRVI AINLRGAWLC TKHAAPRMIE RGGGAIVNLS SLAGQVAVGG TGAYGMSKAG IIQLSRITAA ELRSSGIRSN TLLPAFVDTP MQQTAMAMFD GALGAGGARS MIARLQGRMA APEEMAGIVV FLLSDDASMI TGTTQIADGG TIAALW
NT Sequence
TTGAACCATC CAGACCTGGC CGGCAAGGTC GCGATCGTTA CTGGGGCGGG CGCCGGAATC GGTCTGGCGG TTGCCCGGCG ACTCGCCGAC GAGGGCTGCC ATGTGCTGTG CGCGGACATC GATGGTGATG CCGCGGATGC CGCGGCCACC AAAATCGGTT GTGGCGCAGC GGCCTGCCGG GTTGACGTCA GCGACGAACA ACAGATCATC GCCATGGTCG ACGCCTGTGT TGCCGCGTTC GGCGGGGTGG ACAAGTTGGT CGCCAACGCC GGTGTCGTTC ATCTGGCTTC GCTCATCGAC ACCACCGTCG AGGACTTCGA TCGGGTCATC GCGATCAATC TCCGCGGCGC CTGGCTGTGC ACCAAGCATG CGGCACCGCG GATGATCGAG CGCGGCGGGG GAGCCATTGT CAACCTGTCG TCGTTAGCGG GCCAGGTAGC GGTGGGCGGC ACCGGCGCAT ACGGCATGTC GAAGGCCGGC ATCATCCAGC TCAGCCGCAT CACCGCCGCC GAACTGCGCT CGTCGGGCAT CCGCTCCAAC ACGCTGCTGC CCGCATTCGT CGACACCCCG ATGCAGCAGA CCGCCATGGC AATGTTCGAC GGGGCCCTGG GCGCGGGGGG TGCGCGCTCG ATGATTGCCC GGCTGCAGGG CCGCATGGCC GCACCGGAGG AGATGGCCGG CATCGTGGTG TTCCTGCTGT CCGACGATGC GTCGATGATC ACCGGCACCA CCCAGATCGC CGACGGCGGG ACGATTGCCG CGCTGTGG
Details for MytuD.00472.a.A1.GE25846
| HARVESTED ON: | 11/19/2008 |
| SEQUENCED ON: | 8/18/2010 |
| EXPECTED MW: | 28kDa |
| OBSERVED MW: | 30kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | Low Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMLNHPDLAG KVAIVTGAGA GIGLAVARRL ADEGCHVLCA DIDGDAADAA ATKIGCGAAA CRVDVSDEQQ IIAMVDACVA AFGGVDKLVA NAGVVHLASL IDTTVEDFDR VIAINLRGAW LCTKHAAPRM IERGGGAIVN LSSLAGQVAV GGTGAYGMSK AGIIQLSRIT AAELRSSGIR SNTLLPAFVD TPMQQTAMAM FDGALGAGGA RSMIARLQGR MAAPEEMAGI VVFLLSDDAS MITGTTQIAD GGTIAALW
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgttga accatccaga cctggccggc aaggtcgcga tcgttactgg ggcgggcgcc ggaatcggtc tggcggttgc ccggcgactc gccgacgagg gctgccatgt gctgtgcgcg gacatcgatg gtgatgccgc ggatgccgcg gccaccaaaa tcggttgtgg cgcagcggcc tgccgggttg acgtcagcga cgaacaacag atcatcgcca tggtcgacgc ctgtgttgcc gcgttcggcg gggtggacaa gttggtcgcc aacgccggtg tcgttcatct ggcttcgctc atcgacacca ccgtcgagga cttcgatcgg gtcatcgcga tcaatctccg cggcgcctgg ctgtgcacca agcatgcggc accgcggatg atcgagcgcg gcgggggagc cattgtcaac ctgtcgtcgt tagcgggcca ggtagcggtg ggcggcaccg gcgcatacgg catgtcgaag gccggcatca tccagctcag ccgcatcacc gccgccgaac tgcgctcgtc gggcatccgc tccaacacgc tgctgcccgc attcgtcgac accccgatgc agcagaccgc catggcaatg ttcgacgggg ccctgggcgc ggggggtgcg cgctcgatga ttgcccggct gcagggccgc atggccgcac cggaggagat ggccggcatc gtggtgttcc tgctgtccga cgatgcgtcg atgatcaccg gcaccaccca gatcgccgac ggcgggacga ttgccgcgct gtggtaataa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMLNHPDLAG KVAIVTGAGA GIGLAVARRL ADEGCHVLCA DIDGDAADAA ATKIGCGAAA CRVDVSDEQQ IIAMVDACVA AFGGVDKLVA NAGVVHLASL IDTTVEDFDR VIAINLRGAW LCTKHAAPRM IERGGGAIVN LSSLAGQVAV GGTGAYGMSK AGIIQLSRIT AAELRSSGIR SNTLLPAFVD TPMQQTAMAM FDGALGAGGA RSMIARLQGR MAAPEEMAGI VVFLLSDDAS MITGTTQIAD GGTIAALW
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gttgaaccat ccagacctgg ccggcaaggt cgcgatcgtt actggggcgg gcgccggaat cggtctggcg gttgcccggc gactcgccga cgagggctgc catgtgctgt gcgcggacat cgatggtgat gccgcggatg ccgcggccac caaaatcggt tgtggcgcag cggcctgccg ggttgacgtc agcgacgaac aacagatcat cgccatggtc gacgcctgtg ttgccgcgtt cggcggggtg gacaagttgg tcgccaacgc cggtgtcgtt catctggctt cgctcatcga caccaccgtc gaggacttcg atcgggtcat cgcgatcaat ctccgcggcg cctggctgtg caccaagcat gcggcaccgc ggatgatcga gcgcggcggg ggagccattg tcaacctgtc gtcgttagcg ggccaggtag cggtgggcgg caccggcgca tacggcatgt cgaaggccgg catcatccag ctcagccgca tcaccgccgc cgaactgcgc tcgtcgggca tccgctccaa cacgctgctg cccgcattcg tcgacacccc gatgcagcag accgccatgg caatgttcga cggggccctg ggcgcggggg gtgcgcgctc gatgattgcc cggctgcagg gccgcatggc cgcaccggag gagatggccg gcatcgtggt gttcctgctg tccgacgatg cgtcgatgat caccggcacc acccagatcg ccgacggcgg gacgattgcc gcgctgtgga aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a
Details for MytuD.00472.a.A1.PW25905
| PURIFICATION DATe: | 2/17/2009 |
| CONCENTRATION: | 11.76mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | Low Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 2 |
| VIAL VOLUME: | 100µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMLNHPDLAG KVAIVTGAGA GIGLAVARRL ADEGCHVLCA DIDGDAADAA ATKIGCGAAA CRVDVSDEQQ IIAMVDACVA AFGGVDKLVA NAGVVHLASL IDTTVEDFDR VIAINLRGAW LCTKHAAPRM IERGGGAIVN LSSLAGQVAV GGTGAYGMSK AGIIQLSRIT AAELRSSGIR SNTLLPAFVD TPMQQTAMAM FDGALGAGGA RSMIARLQGR MAAPEEMAGI VVFLLSDDAS MITGTTQIAD GGTIAALW
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgttga accatccaga cctggccggc aaggtcgcga tcgttactgg ggcgggcgcc ggaatcggtc tggcggttgc ccggcgactc gccgacgagg gctgccatgt gctgtgcgcg gacatcgatg gtgatgccgc ggatgccgcg gccaccaaaa tcggttgtgg cgcagcggcc tgccgggttg acgtcagcga cgaacaacag atcatcgcca tggtcgacgc ctgtgttgcc gcgttcggcg gggtggacaa gttggtcgcc aacgccggtg tcgttcatct ggcttcgctc atcgacacca ccgtcgagga cttcgatcgg gtcatcgcga tcaatctccg cggcgcctgg ctgtgcacca agcatgcggc accgcggatg atcgagcgcg gcgggggagc cattgtcaac ctgtcgtcgt tagcgggcca ggtagcggtg ggcggcaccg gcgcatacgg catgtcgaag gccggcatca tccagctcag ccgcatcacc gccgccgaac tgcgctcgtc gggcatccgc tccaacacgc tgctgcccgc attcgtcgac accccgatgc agcagaccgc catggcaatg ttcgacgggg ccctgggcgc ggggggtgcg cgctcgatga ttgcccggct gcagggccgc atggccgcac cggaggagat ggccggcatc gtggtgttcc tgctgtccga cgatgcgtcg atgatcaccg gcaccaccca gatcgccgac ggcgggacga ttgccgcgct gtggtaataa
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMLNHPDLAG KVAIVTGAGA GIGLAVARRL ADEGCHVLCA DIDGDAADAA ATKIGCGAAA CRVDVSDEQQ IIAMVDACVA AFGGVDKLVA NAGVVHLASL IDTTVEDFDR VIAINLRGAW LCTKHAAPRM IERGGGAIVN LSSLAGQVAV GGTGAYGMSK AGIIQLSRIT AAELRSSGIR SNTLLPAFVD TPMQQTAMAM FDGALGAGGA RSMIARLQGR MAAPEEMAGI VVFLLSDDAS MITGTTQIAD GGTIAALW
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GTTGAACCAT CCAGACCTGG CCGGCAAGGT CGCGATCGTT ACTGGGGCGG GCGCCGGAAT CGGTCTGGCG GTTGCCCGGC GACTCGCCGA CGAGGGCTGC CATGTGCTGT GCGCGGACAT CGATGGTGAT GCCGCGGATG CCGCGGCCAC CAAAATCGGT TGTGGCGCAG CGGCCTGCCG GGTTGACGTC AGCGACGAAC AACAGATCAT CGCCATGGTC GACGCCTGTG TTGCCGCGTT CGGCGGGGTG GACAAGTTGG TCGCCAACGC CGGTGTCGTT CATCTGGCTT CGCTCATCGA CACCACCGTC GAGGACTTCG ATCGGGTCAT CGCGATCAAT CTCCGCGGCG CCTGGCTGTG CACCAAGCAT GCGGCACCGC GGATGATCGA GCGCGGCGGG GGAGCCATTG TCAACCTGTC GTCGTTAGCG GGCCAGGTAG CGGTGGGCGG CACCGGCGCA TACGGCATGT CGAAGGCCGG CATCATCCAG CTCAGCCGCA TCACCGCCGC CGAACTGCGC TCGTCGGGCA TCCGCTCCAA CACGCTGCTG CCCGCATTCG TCGACACCCC GATGCAGCAG ACCGCCATGG CAATGTTCGA CGGGGCCCTG GGCGCGGGGG GTGCGCGCTC GATGATTGCC CGGCTGCAGG GCCGCATGGC CGCACCGGAG GAGATGGCCG GCATCGTGGT GTTCCTGCTG TCCGACGATG CGTCGATGAT CACCGGCACC ACCCAGATCG CCGACGGCGG GACGATTGCC GCGCTGTGGA AACAGCACGA ACAAGTTCTG CAGCCAAGCT TCTCGAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA A