MymaA.00472.a

Short-chain type dehydrogenase/reductase

CENTER ID: MymaA.00472.a
ORGANISM: Mycobacterium marinum ATCC BAA-535 / M
ASSOCIATED DISEASE: Cutaneous ulcerations, deep abscesses
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MymaA.00472.a.A1.GE30030 Full length( MymaA.00472.a ) 1 256
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216594.6.peg.3064
RefSeq: YP_001851164.1
UniProt: B2HE29

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRYPDLAGKA TIVTGAGAGI GLAIAERLAA EGAAVLCADI DGASAEQAAA KIGSGAVGYQ VDVSAEEQVA GMVEACVTSF GGLDKLVANA GVVHLAPILD TAVEDFDRVI GINLRGAWLC TKHAAPKMVE RGGGAIVNMS SLAGHIGVGG TAAYGMSKAG ISHLSRVTAA ELRSANVRSN ALLPAFVDTP MQQSAMAMFD EALGTGGANT MIARLQGRMA RPEEMASIVA FLLSDDSSMI TGTTQIADGG TIAALW
NT Sequence
GTGAGGTACC CGGACCTGGC CGGCAAAGCC ACGATCGTGA CCGGGGCCGG GGCCGGGATC GGCCTCGCGA TCGCCGAGCG GCTGGCCGCC GAAGGCGCCG CGGTACTCTG CGCGGACATC GACGGCGCTT CGGCCGAGCA AGCAGCGGCA AAAATCGGCT CCGGCGCAGT CGGGTATCAG GTCGATGTCA GTGCCGAGGA ACAGGTCGCC GGCATGGTCG AAGCCTGCGT CACATCGTTC GGCGGGCTCG ACAAGCTCGT CGCCAATGCC GGAGTCGTTC ATCTGGCCCC GATCCTCGAC ACCGCGGTCG AGGACTTTGA TCGGGTCATC GGCATCAACC TGCGTGGAGC CTGGTTGTGC ACCAAGCATG CGGCACCCAA GATGGTCGAG CGCGGCGGGG GAGCGATCGT CAACATGTCG TCACTGGCGG GCCATATCGG GGTGGGCGGA ACCGCGGCGT ACGGCATGTC GAAGGCCGGG ATCAGCCACC TCAGCCGAGT CACGGCCGCC GAACTGCGCT CGGCCAATGT GCGCTCCAAT GCGTTGTTGC CCGCGTTCGT GGATACCCCC ATGCAACAGA GTGCGATGGC GATGTTCGAC GAGGCCCTAG GAACGGGGGG CGCGAACACC ATGATTGCCA GGCTGCAGGG CCGCATGGCC AGACCCGAGG AGATGGCTAG CATCGTGGCG TTCCTGCTGT CCGACGATTC GTCGATGATT ACCGGAACAA CCCAGATCGC GGACGGCGGA ACCATCGCCG CACTGTGG
Details for MymaA.00472.a.A1.GE30030
HARVESTED ON: 8/10/2010
SEQUENCED ON: 8/16/2010
EXPECTED MW: 28kDa
OBSERVED MW: 28kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 97
PERCENT COVERAGE: 110
Validated AA Sequence
MGTLEAQTQG PGSMVRYPDL AGKATIVTGA GAGIGLAIAE RLAAEGAAVL CADIDGASAE QAAAKIGSGA VGYQVDVSAE EQVAGMVEAC VTSFGGLDKL VANAGVVHLA PILDTAVEDF DRVIGINLRG AWLCTKHAAP KMVERGGGAI VNMSSLAGHI GVGGTAAYGM SKAGISHLSR VTAAELRSAN VRSNALLPAF VDTPMQQSAM AMFDEALGTG GANTMIARLQ GRMARPEEMA SIVAFLLSDD SSMITGTTQI AXXXNHRRTV INXTNKFCSQ ASRGSXANKA RXEXELAAAX AXQXXX
Validated NT Sequence
attttntntn ntttnanaag gaganntnca tggnctcatc accntcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggtga ggtacccgga cctggccggc aaagccacga tcgtgaccgg ggccggggcc gggatcggcc tcgcgatcgc cgagcggctg gccgccgaag gcgccgcggt actctgcgcg gacatcgacg gcgcttcggc cgagcaagca gcggcaaaaa tcggctccgg cgcagtcggg tatcaggtcg atgtcagtgc cgaggaacag gtcgccggca tggtcgaagc ctgcgtcaca tcgttcggcg ggctcgacaa gctcgtcgcc aatgccggag tcgttcatct ggccccgatc ctcgacaccg cggtcgagga ctttgatcgg gtcatcggca tcaacctgcg tggagcctgg ttgtgcacca agcatgcggc acccaagatg gtcgagcgcg gcgggggagc gatcgtcaac atgtcgtcac tggcgggcca tatcggggtg ggcggaaccg cggcgtacgg catgtcgaag gccgggatca gccacctcag ccgagtcacg gccgccgaac tgcgctcggc caatgtgcgc tccaatgcgt tgttgcccgc gttcgtggat acccccatgc aacagagtgc gatggcgatg ttcgacgagg ccctaggaac ggggggcgcg aacaccatga ttgccaggct gcagggccgc atggccagac ccgaggagat ggctagcatc gtggcgttcc tgctgtccga cgattcgtcg atgattaccg gaacaaccca gatcgcggan ngcnggaacc atcgccgcac tgtaataaac ancacgaaca agttctgcag ccaagcttcn cgaggatcnn ntgctaacaa agcccgaang gaagntgagt tggctgctgc cnccgctnan caaanaantn nnnna
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVRYPDLAG KATIVTGAGA GIGLAIAERL AAEGAAVLCA DIDGASAEQA AAKIGSGAVG YQVDVSAEEQ VAGMVEACVT SFGGLDKLVA NAGVVHLAPI LDTAVEDFDR VIGINLRGAW LCTKHAAPKM VERGGGAIVN MSSLAGHIGV GGTAAYGMSK AGISHLSRVT AAELRSANVR SNALLPAFVD TPMQQSAMAM FDEALGTGGA NTMIARLQGR MARPEEMASI VAFLLSDDSS MITGTTQIAD GGTIAAL
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgaggtac ccggacctgg ccggcaaagc cacgatcgtg accggggccg gggccgggat cggcctcgcg atcgccgagc ggctggccgc cgaaggcgcc gcggtactct gcgcggacat cgacggcgct tcggccgagc aagcagcggc aaaaatcggc tccggcgcag tcgggtatca ggtcgatgtc agtgccgagg aacaggtcgc cggcatggtc gaagcctgcg tcacatcgtt cggcgggctc gacaagctcg tcgccaatgc cggagtcgtt catctggccc cgatcctcga caccgcggtc gaggactttg atcgggtcat cggcatcaac ctgcgtggag cctggttgtg caccaagcat gcggcaccca agatggtcga gcgcggcggg ggagcgatcg tcaacatgtc gtcactggcg ggccatatcg gggtgggcgg aaccgcggcg tacggcatgt cgaaggccgg gatcagccac ctcagccgag tcacggccgc cgaactgcgc tcggccaatg tgcgctccaa tgcgttgttg cccgcgttcg tggatacccc catgcaacag agtgcgatgg cgatgttcga cgaggcccta ggaacggggg gcgcgaacac catgattgcc aggctgcagg gccgcatggc cagacccgag gagatggcta gcatcgtggc gttcctgctg tccgacgatt cgtcgatgat taccggaaca acccagatcg cggacggcgg aaccatcgcc gcactgaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa