MytuD.00191.a

NADH dehydrogenase subunit D

CENTER ID: MytuD.00191.a
ORGANISM: Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE: Tuberculosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MytuD.00191.a.B1.GE27153 Full length( MytuD.00191.a ) 1 440
MytuD.00191.a.B1.GE27580 Full length( MytuD.00191.a ) 1 440
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|83332.12.peg.3514
RefSeq: NP_217664.1
TubercuList: Rv3148
MTB Network Portal: Rv3148
UniProt: P9WJH5

Orthologues for Rv3148

Species % Identity % Coverage Clones Proteins Structures
avium 90 100 0 0 0
smegmatis 82 98 1 0 0
thermoresistibile 82 97 0 0 0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTAIADSAGG AGETVLVAGG QDWQQVVDAA RSADPGERIV VNMGPQHPST HGVLRLILEI EGETVVEARC GIGYLHTGIE KNLEYRYWTQ GVTFVTRMDY LSPFFNETAY CLGVEKLLGI TDEIPERVNV IRVLMMELNR ISSHLVALAT GGMELGAMTP MFVGFRAREI VLTLFEKITG LRMNSAYIRP GGVAQDLPPN AATEIAEALK QLRQPLREMG ELLNENAIWK ARTQGVGYLD LTGCMALGIT GPILRSTGLP HDLRKSEPYC GYQHYEFDVI TDDSCDAYGR YMIRVKEMWE SMKIVEQCLD KLRPGPTMIS DRKLAWPADL QVGPDGLGNS PKHIAKIMGS SMEALIHHFK LVTEGIRVPA GQVYVAVESP RGELGVHMVS DGGTRPYRVH YRDPSFTNLQ SVAAMCEGGM VADLIAAVAS IDPVMGGVDR
NT Sequence
ATGACGGCAA TCGCCGACTC GGCTGGCGGC GCCGGCGAGA CCGTCCTGGT CGCTGGCGGG CAGGACTGGC AGCAGGTCGT GGACGCCGCG CGCAGCGCGG ATCCCGGTGA ACGCATCGTC GTCAACATGG GGCCCCAGCA CCCGTCTACC CACGGGGTGT TGCGGTTAAT CCTGGAGATC GAGGGCGAAA CAGTCGTCGA AGCCCGGTGC GGAATCGGCT ACCTGCACAC CGGAATCGAG AAGAACCTCG AATACCGGTA CTGGACCCAG GGCGTCACCT TCGTGACCCG AATGGATTAC CTGTCACCGT TTTTCAACGA AACCGCCTAC TGCCTCGGCG TGGAGAAGCT GCTCGGCATC ACCGATGAGA TACCCGAGCG GGTCAACGTC ATCCGCGTGC TGATGATGGA GCTCAACCGG ATCTCGTCGC ATTTGGTCGC ATTGGCGACC GGGGGCATGG AATTGGGCGC CATGACTCCG ATGTTCGTCG GCTTCCGGGC ACGCGAGATC GTGCTCACGC TGTTCGAAAA GATCACCGGT TTGCGGATGA ACAGCGCCTA CATCCGACCC GGCGGCGTGG CGCAGGACTT ACCGCCCAAC GCGGCCACCG AAATCGCGGA AGCACTCAAG CAGTTGCGCC AACCACTGCG CGAAATGGGC GAGCTGCTCA ACGAAAACGC CATCTGGAAG GCCCGCACCC AGGGCGTCGG ATACCTGGAT CTGACCGGAT GCATGGCACT GGGCATCACC GGCCCGATAC TGCGTTCCAC TGGGTTGCCC CACGACCTGC GGAAAAGCGA GCCCTACTGC GGATACCAGC ACTATGAATT CGATGTGATC ACCGACGACA GCTGTGATGC CTACGGGCGC TACATGATTC GCGTCAAAGA GATGTGGGAG TCGATGAAGA TCGTGGAGCA GTGTCTGGAC AAGTTACGAC CCGGCCCGAC CATGATCTCC GATCGCAAGC TCGCCTGGCC GGCCGACCTG CAGGTGGGGC CCGACGGCCT GGGCAACTCA CCCAAGCACA TCGCCAAAAT CATGGGCTCC TCGATGGAAG CGCTGATCCA CCACTTCAAA CTGGTCACCG AGGGCATCCG GGTGCCGGCG GGCCAGGTCT ACGTCGCGGT GGAGTCCCCC CGTGGTGAGC TCGGCGTACA CATGGTCAGC GACGGTGGCA CCCGCCCCTA CCGGGTGCAC TACCGGGATC CCTCCTTCAC CAACCTGCAG TCCGTCGCCG CGATGTGCGA AGGCGGGATG GTCGCCGATT TGATCGCGGC GGTCGCCAGC ATTGACCCGG TCATGGGCGG GGTGGACCGG
Details for MytuD.00191.a.B1.GE27153
HARVESTED ON: 7/20/2009
SEQUENCED ON: 7/28/2009
EXPECTED MW: 49kDa
OBSERVED MW: 49kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMT AIADSAGGAG ETVLVAGGQD WQQVVDAARS ADPGERIVVN MGPQHPSTHG VLRLILEIEG ETVVEARCGI GYLHTGIEKN LEYRYWTQGV TFVTRMDYLS PFFNETAYCL GVEKLLGITD EIPERVNVIR VLMMELNRIS SHLVALATGG MELGAMTPMF VGFRAREIVL TLFEKITGLR MNSAYIRPGG VAQDLPPNAA TEIAEALKQL RQPLREMGEL LNENAIWKAR TQGVGYLDLT GCMALGITGP ILRSTGLPHD LRKSEPYCGY QHYEFDVITD DSCDAYGRYM IRVKEMWESM KIVEQCLDKL RPGPTMISDR KLAWPADLQV GPDGLGNSPK HIAKIMGSSM EALIHHFKLV TEGIRVPAGQ VYVAVESPRG ELGVHMVSDG GTRPYRVHYR DPSFTNLQSV AAMCEGGMVA DLIAAVASID PVMGGVDR
Validated NT Sequence
atggctcacc accaccacca ccatatgacg gcaatcgccg actcggctgg cggcgccggc gagaccgtcc tggtcgctgg cgggcaggac tggcagcagg tcgtggacgc cgcgcgcagc gcggatcccg gtgaacgcat cgtcgtcaac atggggcccc agcacccgtc tacccacggg gtgttgcggt taatcctgga gatcgagggc gaaacagtcg tcgaagcccg gtgcggaatc ggctacctgc acaccggaat cgagaagaac ctcgaatacc ggtactggac ccagggcgtc accttcgtga cccgaatgga ttacctgtca ccgtttttca acgaaaccgc ctactgcctc ggcgtggaga agctgctcgg catcaccgat gagatacccg agcgggtcaa cgtcatccgc gtgctgatga tggagctcaa ccggatctcg tcgcatttgg tcgcattggc gaccgggggc atggaattgg gcgccatgac tccgatgttc gtcggcttcc gggcacgcga gatcgtgctc acgctgttcg aaaagatcac cggtttgcgg atgaacagcg cctacatccg acccggcggc gtggcgcagg acttaccgcc caacgcggcc accgaaatcg cggaagcact caagcagttg cgccaaccac tgcgcgaaat gggcgagctg ctcaacgaaa acgccatctg gaaggcccgc acccagggcg tcggatacct ggatctgacc ggatgcatgg cactgggcat caccggcccg atactgcgtt ccactgggtt gccccacgac ctgcggaaaa gcgagcccta ctgcggatac cagcactatg aattcgatgt gatcaccgac gacagctgtg atgcctacgg gcgctacatg attcgcgtca aagagatgtg ggagtcgatg aagatcgtgg agcagtgtct ggacaagtta cgacccggcc cgaccatgat ctccgatcgc aagctcgcct ggccggccga cctgcaggtg gggcccgacg gcctgggcaa ctcacccaag cacatcgcca aaatcatggg ctcctcgatg gaagcgctga tccaccactt caaactggtc accgagggca tccgggtgcc ggcgggccag gtctacgtcg cggtggagtc cccccgtggt gagctcggcg tacacatggt cagcgacggt ggcacccgcc cctaccgggt gcactaccgg gatccctcct tcaccaacct gcagtccgtc gccgcgatgt gcgaaggcgg gatggtcgcc gatttgatcg cggcggtcgc cagcattgac ccggtcatgg gcggggtgga ccggtaagtg agtaagat
Expected Protein Sequence
MAHHHHHHMT AIADSAGGAG ETVLVAGGQD WQQVVDAARS ADPGERIVVN MGPQHPSTHG VLRLILEIEG ETVVEARCGI GYLHTGIEKN LEYRYWTQGV TFVTRMDYLS PFFNETAYCL GVEKLLGITD EIPERVNVIR VLMMELNRIS SHLVALATGG MELGAMTPMF VGFRAREIVL TLFEKITGLR MNSAYIRPGG VAQDLPPNAA TEIAEALKQL RQPLREMGEL LNENAIWKAR TQGVGYLDLT GCMALGITGP ILRSTGLPHD LRKSEPYCGY QHYEFDVITD DSCDAYGRYM IRVKEMWESM KIVEQCLDKL RPGPTMISDR KLAWPADLQV GPDGLGNSPK HIAKIMGSSM EALIHHFKLV TEGIRVPAGQ VYVAVESPRG ELGVHMVSDG GTRPYRVHYR DPSFTNLQSV AAMCEGGMVA DLIAAVASID PVMGGVDR
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgacgg caatcgccga ctcggctggc ggcgccggcg agaccgtcct ggtcgctggc gggcaggact ggcagcaggt cgtggacgcc gcgcgcagcg cggatcccgg tgaacgcatc gtcgtcaaca tggggcccca gcacccgtct acccacgggg tgttgcggtt aatcctggag atcgagggcg aaacagtcgt cgaagcccgg tgcggaatcg gctacctgca caccggaatc gagaagaacc tcgaataccg gtactggacc cagggcgtca ccttcgtgac ccgaatggat tacctgtcac cgtttttcaa cgaaaccgcc tactgcctcg gcgtggagaa gctgctcggc atcaccgatg agatacccga gcgggtcaac gtcatccgcg tgctgatgat ggagctcaac cggatctcgt cgcatttggt cgcattggcg accgggggca tggaattggg cgccatgact ccgatgttcg tcggcttccg ggcacgcgag atcgtgctca cgctgttcga aaagatcacc ggtttgcgga tgaacagcgc ctacatccga cccggcggcg tggcgcagga cttaccgccc aacgcggcca ccgaaatcgc ggaagcactc aagcagttgc gccaaccact gcgcgaaatg ggcgagctgc tcaacgaaaa cgccatctgg aaggcccgca cccagggcgt cggatacctg gatctgaccg gatgcatggc actgggcatc accggcccga tactgcgttc cactgggttg ccccacgacc tgcggaaaag cgagccctac tgcggatacc agcactatga attcgatgtg atcaccgacg acagctgtga tgcctacggg cgctacatga ttcgcgtcaa agagatgtgg gagtcgatga agatcgtgga gcagtgtctg gacaagttac gacccggccc gaccatgatc tccgatcgca agctcgcctg gccggccgac ctgcaggtgg ggcccgacgg cctgggcaac tcacccaagc acatcgccaa aatcatgggc tcctcgatgg aagcgctgat ccaccacttc aaactggtca ccgagggcat ccgggtgccg gcgggccagg tctacgtcgc ggtggagtcc ccccgtggtg agctcggcgt acacatggtc agcgacggtg gcacccgccc ctaccgggtg cactaccggg atccctcctt caccaacctg cagtccgtcg ccgcgatgtg cgaaggcggg atggtcgccg atttgatcgc ggcggtcgcc agcattgacc cggtcatggg cggggtggac cggtgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat
Details for MytuD.00191.a.B1.GE27580
HARVESTED ON: 8/17/2009
SEQUENCED ON: 9/29/2009
EXPECTED MW: 49kDa
OBSERVED MW: 45kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMT AIADSAGGAG ETVLVAGGQD WQQVVDAARS ADPGERIVVN MGPQHPSTHG VLRLILEIEG ETVVEARCGI GYLHTGIEKN LEYRYWTQGV TFVTRMDYLS PFFNETAYCL GVEKLLGITD EIPERVNVIR VLMMELNRIS SHLVALATGG MELGAMTPMF VGFRAREIVL TLFEKITGLR MNSAYIRPGG VAQDLPPNAA TEIAEALKQL RQPLREMGEL LNENAIWKAR TQGVGYLDLT GCMALGITGP ILRSTGLPHD LRKSEPYCGY QHYEFDVITD DSCDAYGRYM IRVKEMWESM KIVEQCLDKL RPGPTMISDR KLAWPADLQV GPDGLGNSPK HIAKIMGSSM EALIHHFKLV TEGIRVPAGQ VYVAVESPRG ELGVHMVSDG GTRPYRVHYR DPSFTNLQSV AAMCEGGMVA DLIAAVASID PVMGGVDR
Validated NT Sequence
atggctcacc accaccacca ccatatgacg gcaatcgccg actcggctgg cggcgccggc gagaccgtcc tggtcgctgg cgggcaggac tggcagcagg tcgtggacgc cgcgcgcagc gcggatcccg gtgaacgcat cgtcgtcaac atggggcccc agcacccgtc tacccacggg gtgttgcggt taatcctgga gatcgagggc gaaacagtcg tcgaagcccg gtgcggaatc ggctacctgc acaccggaat cgagaagaac ctcgaatacc ggtactggac ccagggcgtc accttcgtga cccgaatgga ttacctgtca ccgtttttca acgaaaccgc ctactgcctc ggcgtggaga agctgctcgg catcaccgat gagatacccg agcgggtcaa cgtcatccgc gtgctgatga tggagctcaa ccggatctcg tcgcatttgg tcgcattggc gaccgggggc atggaattgg gcgccatgac tccgatgttc gtcggcttcc gggcacgcga gatcgtgctc acgctgttcg aaaagatcac cggtttgcgg atgaacagcg cctacatccg acccggcggc gtggcgcagg acttaccgcc caacgcggcc accgaaatcg cggaagcact caagcagttg cgccaaccac tgcgcgaaat gggcgagctg ctcaacgaaa acgccatctg gaaggcccgc acccagggcg tcggatacct ggatctgacc ggatgcatgg cactgggcat caccggcccg atactgcgtt ccactgggtt gccccacgac ctgcggaaaa gcgagcccta ctgcggatac cagcactatg aattcgatgt gatcaccgac gacagctgtg atgcctacgg gcgctacatg attcgcgtca aagagatgtg ggagtcgatg aagatcgtgg agcagtgtct ggacaagtta cgacccggcc cgaccatgat ctccgatcgc aagctcgcct ggccggccga cctgcaggtg gggcccgacg gcctgggcaa ctcacccaag cacatcgcca aaatcatggg ctcctcgatg gaagcgctga tccaccactt caaactggtc accgagggca tccgggtgcc ggcgggccag gtctacgtcg cggtggagtc cccccgtggt gagctcggcg tacacatggt cagcgacggt ggcacccgcc cctaccgggt gcactaccgg gatccctcct tcaccaacct gcagtccgtc gccgcgatgt gcgaaggcgg gatggtcgcc gatttgatcg cggcggtcgc cagcattgac ccggtcatgg gcggggtgga ccggtaagtg agtaagat
Expected Protein Sequence
MAHHHHHHMT AIADSAGGAG ETVLVAGGQD WQQVVDAARS ADPGERIVVN MGPQHPSTHG VLRLILEIEG ETVVEARCGI GYLHTGIEKN LEYRYWTQGV TFVTRMDYLS PFFNETAYCL GVEKLLGITD EIPERVNVIR VLMMELNRIS SHLVALATGG MELGAMTPMF VGFRAREIVL TLFEKITGLR MNSAYIRPGG VAQDLPPNAA TEIAEALKQL RQPLREMGEL LNENAIWKAR TQGVGYLDLT GCMALGITGP ILRSTGLPHD LRKSEPYCGY QHYEFDVITD DSCDAYGRYM IRVKEMWESM KIVEQCLDKL RPGPTMISDR KLAWPADLQV GPDGLGNSPK HIAKIMGSSM EALIHHFKLV TEGIRVPAGQ VYVAVESPRG ELGVHMVSDG GTRPYRVHYR DPSFTNLQSV AAMCEGGMVA DLIAAVASID PVMGGVDR
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgacgg caatcgccga ctcggctggc ggcgccggcg agaccgtcct ggtcgctggc gggcaggact ggcagcaggt cgtggacgcc gcgcgcagcg cggatcccgg tgaacgcatc gtcgtcaaca tggggcccca gcacccgtct acccacgggg tgttgcggtt aatcctggag atcgagggcg aaacagtcgt cgaagcccgg tgcggaatcg gctacctgca caccggaatc gagaagaacc tcgaataccg gtactggacc cagggcgtca ccttcgtgac ccgaatggat tacctgtcac cgtttttcaa cgaaaccgcc tactgcctcg gcgtggagaa gctgctcggc atcaccgatg agatacccga gcgggtcaac gtcatccgcg tgctgatgat ggagctcaac cggatctcgt cgcatttggt cgcattggcg accgggggca tggaattggg cgccatgact ccgatgttcg tcggcttccg ggcacgcgag atcgtgctca cgctgttcga aaagatcacc ggtttgcgga tgaacagcgc ctacatccga cccggcggcg tggcgcagga cttaccgccc aacgcggcca ccgaaatcgc ggaagcactc aagcagttgc gccaaccact gcgcgaaatg ggcgagctgc tcaacgaaaa cgccatctgg aaggcccgca cccagggcgt cggatacctg gatctgaccg gatgcatggc actgggcatc accggcccga tactgcgttc cactgggttg ccccacgacc tgcggaaaag cgagccctac tgcggatacc agcactatga attcgatgtg atcaccgacg acagctgtga tgcctacggg cgctacatga ttcgcgtcaa agagatgtgg gagtcgatga agatcgtgga gcagtgtctg gacaagttac gacccggccc gaccatgatc tccgatcgca agctcgcctg gccggccgac ctgcaggtgg ggcccgacgg cctgggcaac tcacccaagc acatcgccaa aatcatgggc tcctcgatgg aagcgctgat ccaccacttc aaactggtca ccgagggcat ccgggtgccg gcgggccagg tctacgtcgc ggtggagtcc ccccgtggtg agctcggcgt acacatggtc agcgacggtg gcacccgccc ctaccgggtg cactaccggg atccctcctt caccaacctg cagtccgtcg ccgcgatgtg cgaaggcggg atggtcgccg atttgatcgc ggcggtcgcc agcattgacc cggtcatggg cggggtggac cggtgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat