MysmA.00191.a

NADH-quinone oxidoreductase subunit D

CENTER ID: MysmA.00191.a
ORGANISM: Mycobacterium smegmatis ATCC 700084 / mc(2)155
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MysmA.00191.a.A1.GE28580 Full length( MysmA.00191.a ) 1 442
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|246196.19.peg.2036
RefSeq: YP_886421.1
UniProt: A0QU33

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSTSTVPPDG GEKVVVVGGN DWHEVVAAAR AGAAAQAGER IVVNMGPQHP STHGVLRLIL EIEGEIITEA RCGIGYLHTG IEKNLEYRNW TQGVTFVTRM DYLSPFFNET AYCLGVEKLL GITDDIPERA SVIRVMLMEL NRISSHLVAL ATGGMELGAM SAMFYGFRER EEILRVFESI TGLRMNHAYI RPGGLAADLP DDAITQVRRL VEILPKRLKD LEDLLNENYI WKARTVGVGY LDLTGCMALG ITGPILRSTG LPHDLRKAQP YCGYENYEFD VITDDRCDSY GRYIIRVKEM HESVKIVEQC LARLKPGPVM ISDKKLAWPA DLKLGPDGLG NSPEHIAKIM GRSMEGLIHH FKLVTEGIRV PPGQVYVAVE SPRGELGVHM VSDGGTRPYR VHYRDPSFTN LQAVAATCEG GMVADAIAAV ASIDPVMGGV DR
NT Sequence
ATGAGTACAT CGACGGTCCC GCCCGACGGC GGGGAGAAGG TTGTCGTCGT CGGCGGCAAC GACTGGCACG AGGTGGTCGC CGCCGCGCGG GCAGGCGCAG CGGCGCAGGC CGGCGAACGG ATCGTGGTCA ACATGGGCCC GCAGCATCCG TCCACCCACG GTGTGCTGCG CCTGATCCTG GAGATCGAGG GCGAGATCAT CACCGAGGCC CGGTGCGGTA TCGGCTATCT GCACACCGGC ATCGAGAAGA ACCTCGAGTA CCGCAACTGG ACGCAGGGCG TCACGTTCGT CACCCGGATG GACTACCTGT CACCGTTTTT CAACGAGACG GCGTACTGCC TGGGCGTGGA GAAGCTGCTG GGGATCACCG ACGACATCCC CGAGCGCGCC AGCGTGATCC GCGTGATGCT CATGGAACTC AACCGCATCT CGTCGCACCT GGTGGCGCTG GCCACCGGCG GGATGGAACT GGGCGCGATG AGCGCCATGT TCTACGGCTT CCGTGAACGC GAGGAGATCC TGCGGGTCTT CGAGTCGATC ACCGGTCTGC GGATGAACCA CGCCTACATT CGTCCCGGCG GTCTGGCCGC CGACCTGCCC GACGACGCGA TCACGCAGGT GCGCAGGCTG GTCGAGATCC TGCCGAAACG CCTGAAGGAT CTGGAAGATC TGCTCAACGA GAACTACATC TGGAAGGCCC GCACGGTCGG GGTCGGCTAT CTGGATCTCA CCGGCTGTAT GGCGCTGGGC ATCACCGGTC CGATCCTGCG GTCCACCGGT CTGCCCCACG ACCTGCGCAA GGCGCAACCG TACTGTGGTT ACGAGAACTA CGAATTCGAC GTCATCACCG ACGACCGCTG TGACTCCTAC GGCCGGTACA TCATCCGGGT CAAGGAGATG CACGAGTCGG TGAAGATCGT CGAACAGTGC CTGGCCAGGC TGAAGCCTGG GCCGGTGATG ATCAGCGACA AGAAACTGGC GTGGCCGGCC GATCTCAAAC TCGGGCCCGA CGGCCTGGGC AACTCGCCCG AGCACATCGC CAAGATCATG GGCCGCTCCA TGGAGGGCCT GATCCACCAC TTCAAGCTCG TCACCGAAGG CATCCGCGTA CCACCGGGGC AGGTGTACGT CGCAGTCGAA TCCCCGCGCG GTGAGCTCGG CGTGCACATG GTCTCCGACG GCGGAACCCG GCCCTACCGC GTGCACTACC GCGATCCGTC GTTCACCAAT CTGCAAGCGG TGGCGGCGAC GTGCGAGGGC GGCATGGTGG CCGACGCGAT CGCCGCGGTG GCGTCCATCG ACCCCGTGAT GGGCGGAGTG GACCGC
Details for MysmA.00191.a.A1.GE28580
HARVESTED ON: 2/17/2010
SEQUENCED ON: 2/24/2010
EXPECTED MW: 50kDa
OBSERVED MW: 50kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Few (1-10)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 95
Validated AA Sequence
MSTSTVPPDG GEKVVVVGGN DWHEVVAAAR AGAAAQAGER IVVNMGPQHP STHGVLRLIL EIEGEIITEA RCGIGYLHTG IEKNLEYRNW TQGVTFVTRM DYLSPFFNET AYCLGVEKLL GITDDIPERA SVIRVMLMEL NRISSHLVAL ATGGMELGAM SAMFYGFRER EEILRVFESI TGLRMNHAYI RPGGLAADLP DDAITQVRRL VEILPKRLKD LEDLLNENYI WKARTVGVGY LDLTGCMALG ITGPILRSTG LPHDLRKAQP YCGYENYEFD VITDDRCDSY GRYIIRVKEM HESVKIVEQC LARLKPGPVM ISDKKLAWPA DLKLGPDGLG NSPEHIAKIM GRSMEGLIHH FKLVTEGIRV PPGQVYVAVE SPRGELGVHM VSDGGTRPYR VHYRDPSFTN LQAVAATCEG GMVADAIAAV ASIDPVMGGV
Validated NT Sequence
ccactccgcc catcacgggg tcgatggacg ccaccgcggc gatcgcgtcg gccaccatgc cgccctcgca cgtcgccgcc accgcttgca gattggtgaa cgacggatcg cggtagtgca cgcggtaggg ccgggttccg ccgtcggaga ccatgtgcac gccgagctca ccgcgcgggg attcgactgc gacgtacacc tgccccggtg gtacgcggat gccttcggtg acgagcttga agtggtggat caggccctcc atggagcggc ccatgatctt ggcgatgtgc tcgggcgagt tgcccaggcc gtcgggcccg agtttgagat cggccggcca cgccagtttc ttgtcgctga tcatcaccgg cccaggcttc agcctggcca ggcactgttc gacgatcttc accgactcgt gcatctcctt gacccggatg atgtaccggc cgtaggagtc acagcggtcg tcggtgatga cgtcgaattc gtagttctcg taaccacagt acggttgcgc cttgcgcagg tcgtggggca gaccggtgga ccgcaggatc ggaccggtga tgcccagcgc catacagccg gtgagatcca gatagccgac cccgaccgtg cgggccttcc agatgtagtt ctcgttgagc agatcttcca gatccttcag gcgtttcggc aggatctcga ccagcctgcg cacctgcgtg atcgcgtcgt cgggcaggtc ggcggccaga ccgccgggac gaatgtaggc gtggttcatc cgcagaccgg tgatcgactc gaagacccgc aggatctcct cgcgttcacg gaagccgtag aacatggcgc tcatcgcgcc cagttccatc ccgccggtgg ccagcgccac caggtgcgac gagatgcggt tgagttccat gagcatcacg cggatcacgc tggcgcgctc ggggatgtcg tcggtgatcc ccagcagctt ctccacgccc aggcagtacg ccgtctcgtt gaaaaacggt gacaggtagt ccatccgggt gacgaacgtg acgccctgcg tccagttgcg gtactcgagg ttcttctcga tgccggtgtg cagatagccg ataccgcacc gggcctcggt gatgatctcg ccctcgatct ccaggatcag gcgcagcaca ccgtgggtgg acggatgctg cgggcccatg ttgaccacga tccgttcgcc ggcctgcgcc gctgcgcctg cccgcgcggc ggcgaccacc tcgtgccagt cgttgccgcc gacgacgaca accttctccc cgccgtcggg cgggaccgtc gatgtactca tcgaaccagg accctgggtc tgagcttcca gggtaccc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSTSTVPPD GGEKVVVVGG NDWHEVVAAA RAGAAAQAGE RIVVNMGPQH PSTHGVLRLI LEIEGEIITE ARCGIGYLHT GIEKNLEYRN WTQGVTFVTR MDYLSPFFNE TAYCLGVEKL LGITDDIPER ASVIRVMLME LNRISSHLVA LATGGMELGA MSAMFYGFRE REEILRVFES ITGLRMNHAY IRPGGLAADL PDDAITQVRR LVEILPKRLK DLEDLLNENY IWKARTVGVG YLDLTGCMAL GITGPILRST GLPHDLRKAQ PYCGYENYEF DVITDDRCDS YGRYIIRVKE MHESVKIVEQ CLARLKPGPV MISDKKLAWP ADLKLGPDGL GNSPEHIAKI MGRSMEGLIH HFKLVTEGIR VPPGQVYVAV ESPRGELGVH MVSDGGTRPY RVHYRDPSFT NLQAVAATCE GGMVADAIAA VASIDPVMGG VDR
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gagtacatcg acggtcccgc ccgacggcgg ggagaaggtt gtcgtcgtcg gcggcaacga ctggcacgag gtggtcgccg ccgcgcgggc aggcgcagcg gcgcaggccg gcgaacggat cgtggtcaac atgggcccgc agcatccgtc cacccacggt gtgctgcgcc tgatcctgga gatcgagggc gagatcatca ccgaggcccg gtgcggtatc ggctatctgc acaccggcat cgagaagaac ctcgagtacc gcaactggac gcagggcgtc acgttcgtca cccggatgga ctacctgtca ccgtttttca acgagacggc gtactgcctg ggcgtggaga agctgctggg gatcaccgac gacatccccg agcgcgccag cgtgatccgc gtgatgctca tggaactcaa ccgcatctcg tcgcacctgg tggcgctggc caccggcggg atggaactgg gcgcgatgag cgccatgttc tacggcttcc gtgaacgcga ggagatcctg cgggtcttcg agtcgatcac cggtctgcgg atgaaccacg cctacattcg tcccggcggt ctggccgccg acctgcccga cgacgcgatc acgcaggtgc gcaggctggt cgagatcctg ccgaaacgcc tgaaggatct ggaagatctg ctcaacgaga actacatctg gaaggcccgc acggtcgggg tcggctatct ggatctcacc ggctgtatgg cgctgggcat caccggtccg atcctgcggt ccaccggtct gccccacgac ctgcgcaagg cgcaaccgta ctgtggttac gagaactacg aattcgacgt catcaccgac gaccgctgtg actcctacgg ccggtacatc atccgggtca aggagatgca cgagtcggtg aagatcgtcg aacagtgcct ggccaggctg aagcctgggc cggtgatgat cagcgacaag aaactggcgt ggccggccga tctcaaactc gggcccgacg gcctgggcaa ctcgcccgag cacatcgcca agatcatggg ccgctccatg gagggcctga tccaccactt caagctcgtc accgaaggca tccgcgtacc accggggcag gtgtacgtcg cagtcgaatc cccgcgcggt gagctcggcg tgcacatggt ctccgacggc ggaacccggc cctaccgcgt gcactaccgc gatccgtcgt tcaccaatct gcaagcggtg gcggcgacgt gcgagggcgg catggtggcc gacgcgatcg ccgcggtggc gtccatcgac cccgtgatgg gcggagtgga ccgcaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa