SSGCID: Target Page

MytuD.00058.a

Thioredoxin-disulfide reductase (TrxR)

CENTER ID:	MytuD.00058.a
ORGANISM:	Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE:	Tuberculosis
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MytuD.00058.a.B1.GE43500	full length		1	335

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|83332.12.peg.4363
TubercuList:	Rv3913
MTB Network Portal:	Rv3913
UniProt:	P9WHH1

Orthologues for Rv3913

Species	% Identity	% Coverage	Clones	Proteins	Structures
leprae	83	95	1	1	0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTAPPVHDRA HHPVRDVIVI GSGPAGYTAA LYAARAQLAP LVFEGTSFGG ALMTTTDVEN YPGFRNGITG PELMDEMREQ ALRFGADLRM EDVESVSLHG PLKSVVTADG QTHRARAVIL AMGAAARYLQ VPGEQELLGR GVSSCATCDG FFFRDQDIAV IGGGDSAMEE ATFLTRFARS VTLVHRRDEF RASKIMLDRA RNNDKIRFLT NHTVVAVDGD TTVTGLRVRD TNTGAETTLP VTGVFVAIGH EPRSGLVREA IDVDPDGYVL VQGRTTSTSL PGVFAAGDLV DRTYRQAVTA AGSGCAAAID AERWLAEHAA TGEADSTDAL IGAQR

NT Sequence

atgaccgccc cgcctgtcca tgaccgcgca caccaccccg ttcgcgacgt gatcgttatc ggctccggtc ccgcggggta cactgcggcg ctctacgccg cccgtgccca gctggcgccg ctggtcttcg agggcacgtc tttcggcggc gcgctgatga ccaccaccga cgtggagaac tacccgggat ttcgcaacgg catcaccggt ccagagttga tggatgagat gcgggaacag gcgctgcgat tcggcgcgga cctgcgtatg gaagacgtcg agtcggtatc acttcacggg ccgctgaaat cggtcgtcac cgccgacgga cagacccacc gggcccgagc cgtgatcctg gcaatgggcg cacgggcacg ctatctgcag gtgcccggcg aacaggaatt gctcgggcgc ggggtgagct cgtgcgccac ctgcgacgga ttcttcttcc gcgatcagga catcgccgtc atcggcggcg gtgactcggc aatggaggaa gctaccttcc tgacccgatt cgctcgcagt gtgacgctgg tgcatcgccg cgacgagttc cgggcttcca aaatcatgct cgatcgcgcc cgcaacaacg acaagatacg gttcctcacc aaccacaccg tgtgcgcggt ggacggggac accacagtga ccggcttgcg gtaccgcgac accaacaccg gtgccgaaac caccctgccg gtaaccggtg ttttcgtcgc gatcggccac gagccgcggt cgggcttggt gcgcgaggcc atcgacgtcg acccggacgg ctacgtgttg gtgcaggggc gtaccaccag cacctcactg ccgggcgtgt ttgctgccgg cgacctggtg gatcgcacct atcgccaggc ggttaccgca gcgggcagtg gctgcgccgc ggctatcgac gccgagcgct ggctcgccga gcacgcagca accggagaag ctgacagtac cgacgcattg ataggagcac aacgatga

Details for MytuD.00058.a.B1.GE43500

HARVESTED ON:	11/19/2019
SEQUENCED ON:	12/3/2019
EXPECTED MW:	37kDa
OBSERVED MW:	37kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	99
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMT APPVHDRAHH PVRDVIVIGS GPAGYTAALY AARAQLAPLV FEGTSFGGAL MTTTDVENYP GFRNGITGPE LMDEMREQAL RFGADLRMED VESVSLHGPL KSVVTADGQT HRARAVILAM GAAARYLQVP GEQELLGRGV SSCATCDGFF FRDQDIAVIG GGDSAMEEAT FLTRFARSVT LVHRRDEFRA SKIMLDRARN NDKIRFLTNH TVVAVDGDTT VTGLRVRDTN TGAETTLPVT GVFVAIGHEP RSGLVREAID VDPDGYVLVQ GRTTSTSLPG VFAAGDLVDR TYRQAVTAAG SGCAAAIDAE RWLAEHAATG EADSTXALIG AQR

Validated NT Sequence

ccggntccta tcttactcnc ttatcgttgt gctcctatca atgcntcngt actgtcagct tctccggttg ctgcgtgctc ggcgagccag cgctcggcgt cgatagccgc ggcgcagcca ctgcccgctg cggtaaccgc ctggcgatag gtgcgatcca ccaggtcgcc ggcagcgaac acgcccggca gtgaggtgct ggtggtacgc ccctgcacca acacgtagcc gtccgggtcg acgtcgatgg cctcgcgcac caagcccgac cgcggctcgt ggccgatcgc gacgaaaaca ccggttaccg gcagggtggt ttcggcaccg gtgttggtgt cgcgtacccg caagccggtc actgtggtgt ccccgtccac cgcgaccacg gtgtggttgg tgaggaaccg tatcttgtcg ttgttgcggg cgcgatcgag catgattttg gaagcccgga actcgtcgcg gcgatgcacc agcgtcacac tgcgagcgaa tcgggtcagg aaggtagctt cctccattgc cgagtcaccg ccgccgatga cggcgatgtc ctgatcgcgg aagaagaatc cgtcgcaggt ggcgcacgag ctcaccccgc gcccgagcaa ttcctgttcg ccgggcacct gcagatagcg tgccgctgcg cccattgcca ggatcacggc tcgggcccgg tgggtctgtc cgtcggcggt gacgaccgat ttcagcggcc cgtgaagtga taccgactcg acgtcttcca tacgcaggtc cgcgccgaat cgcagcgcct gttcccgcat ctcatccatc aactctggac cggtgatgcc gttgcgaaat cccgggtagt tctccacgtc ggtggtggtc atcagcgcgc cgccgaaaga cgtgccctcg aagaccagcg gcgccagctg ggcacgggcg gcgtagagcg ccgcagtgta ccccgcggga ccggagccga taacgatcac gtcgcgaacg gggtggtgtg cgcggtcatg gacaggcggg gcggtcatat ggtggtggtg gtggtgagcc at

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaccg ccccgcctgt ccatgaccgc gcacaccacc ccgttcgcga cgtgatcgtt atcggctccg gtcccgcggg gtacactgcg gcgctctacg ccgcccgtgc ccagctggcg ccgctggtct tcgagggcac gtctttcggc ggcgcgctga tgaccaccac cgacgtggag aactacccgg gatttcgcaa cggcatcacc ggtccagagt tgatggatga gatgcgggaa caggcgctgc gattcggcgc ggacctgcgt atggaagacg tcgagtcggt atcacttcac gggccgctga aatcggtcgt caccgccgac ggacagaccc accgggcccg agccgtgatc ctggcaatgg gcgcacgggc acgctatctg caggtgcccg gcgaacagga attgctcggg cgcggggtga gctcgtgcgc cacctgcgac ggattcttct tccgcgatca ggacatcgcc gtcatcggcg gcggtgactc ggcaatggag gaagctacct tcctgacccg attcgctcgc agtgtgacgc tggtgcatcg ccgcgacgag ttccgggctt ccaaaatcat gctcgatcgc gcccgcaaca acgacaagat acggttcctc accaaccaca ccgtgtgcgc ggtggacggg gacaccacag tgaccggctt gcggtaccgc gacaccaaca ccggtgccga aaccaccctg ccggtaaccg gtgttttcgt cgcgatcggc cacgagccgc ggtcgggctt ggtgcgcgag gccatcgacg tcgacccgga cggctacgtg ttggtgcagg ggcgtaccac cagcacctca ctgccgggcg tgtttgctgc cggcgacctg gtggatcgca cctatcgcca ggcggttacc gcagcgggca gtggctgcgc cgcggctatc gacgccgagc gctggctcgc cgagcacgca gcaaccggag aagctgacag taccgacgca ttgataggag cacaacgatg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat