MyleA.00058.a

PROBABLE THIOREDOXIN REDUCTASE TRXB2 (TRXR) (TR)

CENTER ID: MyleA.00058.a
ORGANISM: Mycobacterium leprae Br4923
ASSOCIATED DISEASE: Hansen's disease, leprosy
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyleA.00058.a.A1.GE34150 full length 1 458
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyleA.00058.a.A1.PW34792 full length 1 458

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|561304.4.peg.5227
RefSeq: YP_002504354.1
UniProt: A0A0H3MWR3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNTTPSAHET IHEVIVIGSG PAGYTAALYA ARAQLTPLVF EGTSFGGALM TTTEVENYPG FRNGITGPEL MDDMREQALR FGAELRTEDV ESVSLRGPIK SVVTAEGQTY QARAVILAMG TSVRYLQIPG EQELLGRGVS ACATCDGSFF RGQDIAVIGG GDSAMEEALF LTRFARSVTL VHRRDEFRAS KIMLGRARNN DKIKFITNHT VVAVNGYTTV TGLRLRNTTT GEETTLVVTG VFVAIGHEPR SSLVSDVVDI DPDGYVLVKG RTTSTSMDGV FAAGDLVDRT YRQAITAAGS GCAAAIDAER WLAEHAGSKA NETTEETGDV DSTDTTDWST AMTDAKNAGV TIEVTDASFF ADVLSSNKPV LVDFWATWCG PCKMVAPVLE EIASEQRNQL TVAKLDVDTN PEMAREFQVV SIPTMILFQG GQPVKRIVGA KGKAALLRDL SDVVPNLN
NT Sequence
atgaacacca ctccttctgc gcatgagacg atacacgaag tgatcgttat tggctccggt ccagcaggct acactgctgc cctgtacgcc gctcgtgcac agctaacacc gctggtattt gagggtacct cattcggcgg cgcgctgatg accaccaccg aggtggaaaa ctacccaggt tttcgcaacg gcataaccgg cccggagttg atggacgata tgcgtgaaca ggcactgcga ttcggcgcgg aactgcggac cgaagacgtc gagtcggtat cattgcgtgg cccgatcaaa tcggtcgtca ccgctgaagg acagacttat caggcccgag ccgtcatcct cgccatgggt acctccgtgc gttatctaca gatccccggc gagcaagaat tgctaggacg tggcgtgagt gcatgcgcga cctgcgacgg gtcctttttc cgcggccaag acattgccgt cattggcggt ggagactcag cgatggagga agccctcttt ttgacccggt tcgcccgcag cgtcacgctc gtgcaccgcc gcgacgaatt ccgagcttct aagatcatgc tcggtcgcgc ccgtaacaat gacaagatca aattcatcac caaccacacc gtggtcgcgg tgaacgggta tacaacagtg accggattgc ggttgcgtaa caccacaacg ggagaggaaa ccacgctagt agtgaccggg gtttttgttg caattggcca tgaaccacgt tccagcctgg tgagcgatgt cgtcgacata gacccggatg gctacgtcct ggtgaaagga cgtacgacga gtacatcgat ggacggcgtt tttgcggccg gcgacctggt agatcgcacc taccggcagg cgatcactgc cgcaggtagt ggctgtgccg ccgccatcga cgccgaacgt tggttggcgg agcatgccgg gtcaaaagct aacgaaacaa cagaggaaac tggagacgtt gacagtaccg acacaaccga ttggagcact gcgatgactg acgccaagaa cgccggggtc acaatagaag tgaccgatgc ttcctttttc gcagacgtct tatccagtaa taagcctgtg ttagttgatt tttgggcaac atggtgtgga ccctgcaaga tggtagcgcc ggtactcgaa gagatcgcgt ccgaacaacg aaaccagctc actgtcgcca agttagatgt agacaccaac ccggaaatgg cacgcgagtt ccaggtcgtg tcgataccca caatgattct gttccagggt ggccaaccag taaaacgcat cgttggcgct aagggcaaag cagcgttact acgtgacctt tccgacgtgg tacctaacct caattag
Details for MyleA.00058.a.A1.GE34150
HARVESTED ON: 12/6/2011
SEQUENCED ON: 12/8/2011
EXPECTED MW: 51kDa
OBSERVED MW: 51kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 99
PERCENT COVERAGE: 70
Validated AA Sequence
MNTTPSAHET IHEVIVIGSG PAGYTAALYA ARAQLTPLVF EGTSFGGALM TTTEVENYPG FRNGITGPEL MDDMREQALR FGAELRTEDV ESVSLRGPIK SVVTAEGQTY QARAVILAMG TSVRYLQIPG EQELLGRGVS ACATCDGSFF RGQDIAVIGG GDSAMEEALF LTRFARSVTL VHRRDEFRAS KIMLGRARNN DKIKFITNHT VVAVNGYTTV TGXRLRNTTT GEETTLVVTG VFVAIGHEPR SSLVSDVVDI DPDGYVLVKG RTTSTSMDGV FAAGDLVDRT YRQAITAAGS GCAAAIDAER WLAEHAGSKA NETTEETGDV DSTDTT
Validated NT Sequence
tcggttgtgt cggtactgtc aacgtctcca gtttcctctg ttgtttcgtt agcttttgac ccggcatgct ccgccaacca acgttcggcg tcgatggcgg cggcacagcc actacctgcg gcagtgatcg cctgccggta ggtgcgatct accaggtcgc cggccgcaaa aacgccgtcc atcgatgtac tcgtcgtacg tcctttcacc aggacgtagc catccgggtc tatgtcgacg acatcgctca ccaggctgga acgtggttca tggccaattg caacaaaaac cccggtcact actagcgtgg tttcctctcc cgttgtggtg ttacgcaacc gcnatccggt cactgttgta tacccgttca ccgcgaccac ggtgtggttg gtgatgaatt tgatcttgtc attgttacgg gcgcgaccga gcatgatctt agaagctcgg aattcgtcgc ggcggtgcac gagcgtgacg ctgcgggcga accgggtcaa aaagagggct tcctccatcg ctgagtctcc accgccaatg acggcaatgt cttggccgcg gaaaaaggac ccgtcgcagg tcgcgcatgc actcacgcca cgtcctagca attcttgctc gccggggatc tgtagataac gcacggaggt acccatggcg aggatgacgg ctcgggcctg ataagtctgt ccttcagcgg tgacgaccga tttgatcggg ccacgcaatg ataccgactc gacgtcttcg gtccgcagtt ccgcgccgaa tcgcagtgcc tgttcacgca tatcgtccat caactccggg ccggttatgc cgttgcgaaa acctgggtag ttttccacct cggtggtggt catcagcgcg ccgccgaatg aggtaccctc aaataccagc ggtgttagct gtgcacgagc ggcgtacagg gcagcagtgt agcctgctgg accggagcca ataacgatca cttcgtgtat cgtctcatgc gcagaaggag tggtgttcat cgaaccagga ccctgggtct gagcttccag g
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMNTTPSAHE TIHEVIVIGS GPAGYTAALY AARAQLTPLV FEGTSFGGAL MTTTEVENYP GFRNGITGPE LMDDMREQAL RFGAELRTED VESVSLRGPI KSVVTAEGQT YQARAVILAM GTSVRYLQIP GEQELLGRGV SACATCDGSF FRGQDIAVIG GGDSAMEEAL FLTRFARSVT LVHRRDEFRA SKIMLGRARN NDKIKFITNH TVVAVNGYTT VTGLRLRNTT TGEETTLVVT GVFVAIGHEP RSSLVSDVVD IDPDGYVLVK GRTTSTSMDG VFAAGDLVDR TYRQAITAAG SGCAAAIDAE RWLAEHAGSK ANETTEETGD VDSTDTTDWS TAMTDAKNAG VTIEVTDASF FADVLSSNKP VLVDFWATWC GPCKMVAPVL EEIASEQRNQ LTVAKLDVDT NPEMAREFQV VSIPTMILFQ GGQPVKRIVG AKGKAALLRD LSDVVPNLN
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaacaccact ccttctgcgc atgagacgat acacgaagtg atcgttattg gctccggtcc agcaggctac actgctgccc tgtacgccgc tcgtgcacag ctaacaccgc tggtatttga gggtacctca ttcggcggcg cgctgatgac caccaccgag gtggaaaact acccaggttt tcgcaacggc ataaccggcc cggagttgat ggacgatatg cgtgaacagg cactgcgatt cggcgcggaa ctgcggaccg aagacgtcga gtcggtatca ttgcgtggcc cgatcaaatc ggtcgtcacc gctgaaggac agacttatca ggcccgagcc gtcatcctcg ccatgggtac ctccgtgcgt tatctacaga tccccggcga gcaagaattg ctaggacgtg gcgtgagtgc atgcgcgacc tgcgacgggt cctttttccg cggccaagac attgccgtca ttggcggtgg agactcagcg atggaggaag ccctcttttt gacccggttc gcccgcagcg tcacgctcgt gcaccgccgc gacgaattcc gagcttctaa gatcatgctc ggtcgcgccc gtaacaatga caagatcaaa ttcatcacca accacaccgt ggtcgcggtg aacgggtata caacagtgac cggattgcgg ttgcgtaaca ccacaacggg agaggaaacc acgctagtag tgaccggggt ttttgttgca attggccatg aaccacgttc cagcctggtg agcgatgtcg tcgacataga cccggatggc tacgtcctgg tgaaaggacg tacgacgagt acatcgatgg acggcgtttt tgcggccggc gacctggtag atcgcaccta ccggcaggcg atcactgccg caggtagtgg ctgtgccgcc gccatcgacg ccgaacgttg gttggcggag catgccgggt caaaagctaa cgaaacaaca gaggaaactg gagacgttga cagtaccgac acaaccgatt ggagcactgc gatgactgac gccaagaacg ccggggtcac aatagaagtg accgatgctt cctttttcgc agacgtctta tccagtaata agcctgtgtt agttgatttt tgggcaacat ggtgtggacc ctgcaagatg gtagcgccgg tactcgaaga gatcgcgtcc gaacaacgaa accagctcac tgtcgccaag ttagatgtag acaccaaccc ggaaatggca cgcgagttcc aggtcgtgtc gatacccaca atgattctgt tccagggtgg ccaaccagta aaacgcatcg ttggcgctaa gggcaaagca gcgttactac gtgacctttc cgacgtggta cctaacctca attaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa
Details for MyleA.00058.a.A1.PW34792
PURIFICATION DATe: 1/29/2012
CONCENTRATION: 25.52mg/ml
OBSERVED MW: 50kDa
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 4
VIAL VOLUME: 110µl
PERCENT IDENTITY: 99
PERCENT COVERAGE: 70
Protocol Notes
notes unavailable
Validated AA Sequence
MNTTPSAHET IHEVIVIGSG PAGYTAALYA ARAQLTPLVF EGTSFGGALM TTTEVENYPG FRNGITGPEL MDDMREQALR FGAELRTEDV ESVSLRGPIK SVVTAEGQTY QARAVILAMG TSVRYLQIPG EQELLGRGVS ACATCDGSFF RGQDIAVIGG GDSAMEEALF LTRFARSVTL VHRRDEFRAS KIMLGRARNN DKIKFITNHT VVAVNGYTTV TGXRLRNTTT GEETTLVVTG VFVAIGHEPR SSLVSDVVDI DPDGYVLVKG RTTSTSMDGV FAAGDLVDRT YRQAITAAGS GCAAAIDAER WLAEHAGSKA NETTEETGDV DSTDTT
Validated NT Sequence
tcggttgtgt cggtactgtc aacgtctcca gtttcctctg ttgtttcgtt agcttttgac ccggcatgct ccgccaacca acgttcggcg tcgatggcgg cggcacagcc actacctgcg gcagtgatcg cctgccggta ggtgcgatct accaggtcgc cggccgcaaa aacgccgtcc atcgatgtac tcgtcgtacg tcctttcacc aggacgtagc catccgggtc tatgtcgacg acatcgctca ccaggctgga acgtggttca tggccaattg caacaaaaac cccggtcact actagcgtgg tttcctctcc cgttgtggtg ttacgcaacc gcnatccggt cactgttgta tacccgttca ccgcgaccac ggtgtggttg gtgatgaatt tgatcttgtc attgttacgg gcgcgaccga gcatgatctt agaagctcgg aattcgtcgc ggcggtgcac gagcgtgacg ctgcgggcga accgggtcaa aaagagggct tcctccatcg ctgagtctcc accgccaatg acggcaatgt cttggccgcg gaaaaaggac ccgtcgcagg tcgcgcatgc actcacgcca cgtcctagca attcttgctc gccggggatc tgtagataac gcacggaggt acccatggcg aggatgacgg ctcgggcctg ataagtctgt ccttcagcgg tgacgaccga tttgatcggg ccacgcaatg ataccgactc gacgtcttcg gtccgcagtt ccgcgccgaa tcgcagtgcc tgttcacgca tatcgtccat caactccggg ccggttatgc cgttgcgaaa acctgggtag ttttccacct cggtggtggt catcagcgcg ccgccgaatg aggtaccctc aaataccagc ggtgttagct gtgcacgagc ggcgtacagg gcagcagtgt agcctgctgg accggagcca ataacgatca cttcgtgtat cgtctcatgc gcagaaggag tggtgttcat cgaaccagga ccctgggtct gagcttccag g
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMNTTPSAHE TIHEVIVIGS GPAGYTAALY AARAQLTPLV FEGTSFGGAL MTTTEVENYP GFRNGITGPE LMDDMREQAL RFGAELRTED VESVSLRGPI KSVVTAEGQT YQARAVILAM GTSVRYLQIP GEQELLGRGV SACATCDGSF FRGQDIAVIG GGDSAMEEAL FLTRFARSVT LVHRRDEFRA SKIMLGRARN NDKIKFITNH TVVAVNGYTT VTGLRLRNTT TGEETTLVVT GVFVAIGHEP RSSLVSDVVD IDPDGYVLVK GRTTSTSMDG VFAAGDLVDR TYRQAITAAG SGCAAAIDAE RWLAEHAGSK ANETTEETGD VDSTDTTDWS TAMTDAKNAG VTIEVTDASF FADVLSSNKP VLVDFWATWC GPCKMVAPVL EEIASEQRNQ LTVAKLDVDT NPEMAREFQV VSIPTMILFQ GGQPVKRIVG AKGKAALLRD LSDVVPNLN
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAACACCACT CCTTCTGCGC ATGAGACGAT ACACGAAGTG ATCGTTATTG GCTCCGGTCC AGCAGGCTAC ACTGCTGCCC TGTACGCCGC TCGTGCACAG CTAACACCGC TGGTATTTGA GGGTACCTCA TTCGGCGGCG CGCTGATGAC CACCACCGAG GTGGAAAACT ACCCAGGTTT TCGCAACGGC ATAACCGGCC CGGAGTTGAT GGACGATATG CGTGAACAGG CACTGCGATT CGGCGCGGAA CTGCGGACCG AAGACGTCGA GTCGGTATCA TTGCGTGGCC CGATCAAATC GGTCGTCACC GCTGAAGGAC AGACTTATCA GGCCCGAGCC GTCATCCTCG CCATGGGTAC CTCCGTGCGT TATCTACAGA TCCCCGGCGA GCAAGAATTG CTAGGACGTG GCGTGAGTGC ATGCGCGACC TGCGACGGGT CCTTTTTCCG CGGCCAAGAC ATTGCCGTCA TTGGCGGTGG AGACTCAGCG ATGGAGGAAG CCCTCTTTTT GACCCGGTTC GCCCGCAGCG TCACGCTCGT GCACCGCCGC GACGAATTCC GAGCTTCTAA GATCATGCTC GGTCGCGCCC GTAACAATGA CAAGATCAAA TTCATCACCA ACCACACCGT GGTCGCGGTG AACGGGTATA CAACAGTGAC CGGATTGCGG TTGCGTAACA CCACAACGGG AGAGGAAACC ACGCTAGTAG TGACCGGGGT TTTTGTTGCA ATTGGCCATG AACCACGTTC CAGCCTGGTG AGCGATGTCG TCGACATAGA CCCGGATGGC TACGTCCTGG TGAAAGGACG TACGACGAGT ACATCGATGG ACGGCGTTTT TGCGGCCGGC GACCTGGTAG ATCGCACCTA CCGGCAGGCG ATCACTGCCG CAGGTAGTGG CTGTGCCGCC GCCATCGACG CCGAACGTTG GTTGGCGGAG CATGCCGGGT CAAAAGCTAA CGAAACAACA GAGGAAACTG GAGACGTTGA CAGTACCGAC ACAACCGATT GGAGCACTGC GATGACTGAC GCCAAGAACG CCGGGGTCAC AATAGAAGTG ACCGATGCTT CCTTTTTCGC AGACGTCTTA TCCAGTAATA AGCCTGTGTT AGTTGATTTT TGGGCAACAT GGTGTGGACC CTGCAAGATG GTAGCGCCGG TACTCGAAGA GATCGCGTCC GAACAACGAA ACCAGCTCAC TGTCGCCAAG TTAGATGTAG ACACCAACCC GGAAATGGCA CGCGAGTTCC AGGTCGTGTC GATACCCACA ATGATTCTGT TCCAGGGTGG CCAACCAGTA AAACGCATCG TTGGCGCTAA GGGCAAAGCA GCGTTACTAC GTGACCTTTC CGACGTGGTA CCTAACCTCA ATTAAACAGC ACGAACAAGT TCTGCAGCCA AGCTTCTCGA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAA