MysmA.18640.a
Putative uncharacterized protein (Rv0999 ortholog)
| CENTER ID: | MysmA.18640.a |
| ORGANISM: | Mycobacterium smegmatis ATCC 700084 / mc(2)155 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIC |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MysmA.18640.a.B2.GE37635 | mature protein | 23 | 225 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MysmA.18640.a.B2.PS02025 | mature protein | 23 | 225 |
Structures
4TMD
DEPOSITED: 6/1/2014
DETERMINATION: XRay
CLONE: MysmA.18640.a.B2.GE37635
PROTEIN: MysmA.18640.a.B2.PS02025
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| PATRIC ID: | fig|246196.19.peg.5313 |
| RefSeq: | YP_889691.1 |
| UniProt: | A0R3F3 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSNPTRTLAL VTSGLMASAM ALAGCSSDGP AYPDADIAKV AQLKSSFGPE FKVSEVAPTG IDPKLLSPQK LPEGVKFEPA DCAKFAEGQQ FPPGLQGNMA ATAAEGEGNR FIVMAVETSE PVPLSDPGDE CKRVKFLGTG ARGQVDVVES PQIDDARTVG THRIIQTMVQ GQPRTGELYN YVASFDNYMV IVTANPLVLP DKPVAKVDTE RARELLSAAV AAVRG
NT Sequence
atgtccaacc ccacccgcac cctggcactc gtcacctcgg gcctgatggc gtctgccatg gcgctcgcag gctgcagttc ggacgggccg gcgtatcccg acgccgacat cgccaaggtg gctcagctga aatcgtcgtt cggccccgag ttcaaggtca gcgaggtcgc gcccaccggt atcgacccga agttgctgtc accgcagaag ttgcccgagg gtgtgaagtt cgagcccgcc gactgcgcga agttcgccga gggacaacag tttccgccgg gactgcaggg caacatggcc gcgaccgcgg ccgaaggtga gggcaaccgg ttcatcgtga tggccgtgga gacctcggag cccgtcccgc tgagcgatcc cggtgacgag tgcaaacgcg tgaagttcct cgggacgggt gcgcgcgggc aggtcgacgt cgtcgaatcg ccgcagatag acgacgcccg cacggtcggt acgcaccgca tcatccagac gatggtgcag gggcagccgc gcaccggtga gctctacaac tatgtcgcga gcttcgacaa ctacatggtg atcgtgaccg ccaacccgct ggtgctgccc gacaagcccg tggccaaggt cgacaccgaa cgtgcccgtg aactgctttc ggcggccgtg gccgcggtcc ggggataa
Details for MysmA.18640.a.B2.GE37635
| HARVESTED ON: | 1/13/2014 |
| SEQUENCED ON: | 1/9/2014 |
| EXPECTED MW: | 22kDa |
| OBSERVED MW: | 23kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | High Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMA GCSSDGPAYP DADIAKVAQL KSSFGPEFKV SEVAPTGIDP KLLSPQKLPE GVKFEPADCA KFAEGQQFPP GLQGNMAATA AEGEGNRFIV MAVETSEPVP LSDPGDECKR VKFLGTGARG QVDVVESPQI DDARTVGTHR IIQTMVQGQP RTGELYNYVA SFDNYMVIVT ANPLVLPDKP VAKVDTERAR ELLSAAVAAV RG
Validated NT Sequence
cagccggatc ctatcttact cacttatccc cggaccgcgg ccacggccgc cgaaagcagt tcacgggcac gttcggtgtc gaccttggcc acgggcttgt cgggcagcac cagcgggttg gcggtcacga tcaccatgta gttgtcgaag ctcgcgacat agttgtagag ctcaccggtg cgcggctgcc cctgcaccat cgtctggatg atgcggtgcg taccgaccgt gcgggcgtcg tctatctgcg gcgattcgac gacgtcgacc tgcccgcgcg cacccgtccc gaggaacttc acgcgtttgc actcgtcacc gggatcgctc agcgggacgg gctccgaggt ctccacggcc atcacgatga accggttgcc ctcaccttcg gccgcggtcg cggccatgtt gccctgcagt cccggcggaa actgttgtcc ctcggcgaac ttcgcgcagt cggcgggctc gaacttcaca ccctcgggca acttctgcgg tgacagcaac ttcgggtcga taccggtggg cgcgacctcg ctgaccttga actcggggcc gaacgacgat ttcagctgag ccaccttggc gatgtcggcg tcgggatacg ccggcccgtc cgaactgcag cctgccatat ggtggtggtg gtggtgagcc at
Expected Protein Sequence
MAHHHHHHAG CSSDGPAYPD ADIAKVAQLK SSFGPEFKVS EVAPTGIDPK LLSPQKLPEG VKFEPADCAK FAEGQQFPPG LQGNMAATAA EGEGNRFIVM AVETSEPVPL SDPGDECKRV KFLGTGARGQ VDVVESPQID DARTVGTHRI IQTMVQGQPR TGELYNYVAS FDNYMVIVTA NPLVLPDKPV AKVDTERARE LLSAAVAAVR G
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgcaggct gcagttcgga cgggccggcg tatcccgacg ccgacatcgc caaggtggct cagctgaaat cgtcgttcgg ccccgagttc aaggtcagcg aggtcgcgcc caccggtatc gacccgaagt tgctgtcacc gcagaagttg cccgagggtg tgaagttcga gcccgccgac tgcgcgaagt tcgccgaggg acaacagttt ccgccgggac tgcagggcaa catggccgcg accgcggccg aaggtgaggg caaccggttc atcgtgatgg ccgtggagac ctcggagccc gtcccgctga gcgatcccgg tgacgagtgc aaacgcgtga agttcctcgg gacgggtgcg cgcgggcagg tcgacgtcgt cgaatcgccg cagatagacg acgcccgcac ggtcggtacg caccgcatca tccagacgat ggtgcagggg cagccgcgca ccggtgagct ctacaactat gtcgcgagct tcgacaacta catggtgatc gtgaccgcca acccgctggt gctgcccgac aagcccgtgg ccaaggtcga caccgaacgt gcccgtgaac tgctttcggc ggccgtggcc gcggtccggg gatgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat
Details for MysmA.18640.a.B2.PS02025
| PURIFICATION DATe: | 3/26/2014 |
| CONCENTRATION: | 30mg/ml |
| OBSERVED MW: | 26kDa |
| EXPRESSION LEVEL: | Low Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 1 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMA GCSSDGPAYP DADIAKVAQL KSSFGPEFKV SEVAPTGIDP KLLSPQKLPE GVKFEPADCA KFAEGQQFPP GLQGNMAATA AEGEGNRFIV MAVETSEPVP LSDPGDECKR VKFLGTGARG QVDVVESPQI DDARTVGTHR IIQTMVQGQP RTGELYNYVA SFDNYMVIVT ANPLVLPDKP VAKVDTERAR ELLSAAVAAV RG
Validated NT Sequence
cagccggatc ctatcttact cacttatccc cggaccgcgg ccacggccgc cgaaagcagt tcacgggcac gttcggtgtc gaccttggcc acgggcttgt cgggcagcac cagcgggttg gcggtcacga tcaccatgta gttgtcgaag ctcgcgacat agttgtagag ctcaccggtg cgcggctgcc cctgcaccat cgtctggatg atgcggtgcg taccgaccgt gcgggcgtcg tctatctgcg gcgattcgac gacgtcgacc tgcccgcgcg cacccgtccc gaggaacttc acgcgtttgc actcgtcacc gggatcgctc agcgggacgg gctccgaggt ctccacggcc atcacgatga accggttgcc ctcaccttcg gccgcggtcg cggccatgtt gccctgcagt cccggcggaa actgttgtcc ctcggcgaac ttcgcgcagt cggcgggctc gaacttcaca ccctcgggca acttctgcgg tgacagcaac ttcgggtcga taccggtggg cgcgacctcg ctgaccttga actcggggcc gaacgacgat ttcagctgag ccaccttggc gatgtcggcg tcgggatacg ccggcccgtc cgaactgcag cctgccatat ggtggtggtg gtggtgagcc at
Expressed Protein Sequence
MAHHHHHHAG CSSDGPAYPD ADIAKVAQLK SSFGPEFKVS EVAPTGIDPK LLSPQKLPEG VKFEPADCAK FAEGQQFPPG LQGNMAATAA EGEGNRFIVM AVETSEPVPL SDPGDECKRV KFLGTGARGQ VDVVESPQID DARTVGTHRI IQTMVQGQPR TGELYNYVAS FDNYMVIVTA NPLVLPDKPV AKVDTERARE LLSAAVAAVR G
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATGCAGGCT GCAGTTCGGA CGGGCCGGCG TATCCCGACG CCGACATCGC CAAGGTGGCT CAGCTGAAAT CGTCGTTCGG CCCCGAGTTC AAGGTCAGCG AGGTCGCGCC CACCGGTATC GACCCGAAGT TGCTGTCACC GCAGAAGTTG CCCGAGGGTG TGAAGTTCGA GCCCGCCGAC TGCGCGAAGT TCGCCGAGGG ACAACAGTTT CCGCCGGGAC TGCAGGGCAA CATGGCCGCG ACCGCGGCCG AAGGTGAGGG CAACCGGTTC ATCGTGATGG CCGTGGAGAC CTCGGAGCCC GTCCCGCTGA GCGATCCCGG TGACGAGTGC AAACGCGTGA AGTTCCTCGG GACGGGTGCG CGCGGGCAGG TCGACGTCGT CGAATCGCCG CAGATAGACG ACGCCCGCAC GGTCGGTACG CACCGCATCA TCCAGACGAT GGTGCAGGGG CAGCCGCGCA CCGGTGAGCT CTACAACTAT GTCGCGAGCT TCGACAACTA CATGGTGATC GTGACCGCCA ACCCGCTGGT GCTGCCCGAC AAGCCCGTGG CCAAGGTCGA CACCGAACGT GCCCGTGAAC TGCTTTCGGC GGCCGTGGCC GCGGTCCGGG GATGAGTAAG ATAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAATT CTTGAAGACG AAAGGGCCTC GTGATACGCC TATTTTTATA GGTTAATGTC ATGATAATAA TGGTTTCTTA GACGTCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAAGTAA AAGATGCTGA AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT CTCAACAGCG GTAAGATCCT TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC ACTTTTAAAG TTCTGCTATG TGGCGCGGTA TTATCCCGTG TTGACGCCGG GCAAGAGCAA CTCGGTCGCC GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA AAGCATCTTA CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT GATAACACTG CGGCCAACTT ACTTCTGACA ACGATCGGAG GACCGAAGGA GCTAACCGCT TTTTTGCACA ACATGGGGGA TCATGTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC CAAACGACGA GCGTGACACC ACGATGCCTG CAGCAATGGC AACAACGTTG CGCAAACTAT TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG ATGGAGGCGG ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT ATTGCTGATA AATCTGGAGC CGGTGAGCGT GGGTCTCGCG GTATCATTGC AGCACTGGGG CCAGATGGTA AGCCCTCCCG TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACGAA ATAGACAGAT CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG TCAGACCAAG TTTACTCATA TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA AGGATCTAGG TGAAGATCCT TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTCCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCT ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA ACCGTATTAC CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACG ACCGAGCGCA GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC GCCTGATGCG GTATTTTCTC CTTACGCATC TGTGCGGTAT TTCACACCGC ATATATGGTG CACTCTCAGT ACAATCTGCT CTGATGCCGC ATAGTTAAGC CAGTATACAC TCCGCTATCG CTACGTGACT GGGTCATGGC TGCGCCCCGA CACCCGCCAA CACCCGCTGA CGCGCCCTGA CGGGCTTGTC TGCTCCCGGC ATCCGCTTAC AGACAAGCTG TGACCGTCTC CGGGAGCTGC ATGTGTCAGA GGTTTTCACC GTCATCACCG AAACGCGCGA GGCAGCTGCG GTAAAGCTCA TCAGCGTGGT CGTGAAGCGA TTCACAGATG TCTGCCTGTT CATCCGCGTC CAGCTCGTTG AGTTTCTCCA GAAGCGTTAA TGTCTGGCTT CTGATAAAGC GGGCCATGTT AAGGGCGGTT TTTTCCTGTT TGGTCACTGA TGCCTCCGTG TAAGGGGGAT TTCTGTTCAT GGGGGTAATG ATACCGATGA AACGAGAGAG GATGCTCACG ATACGGGTTA CTGATGATGA ACATGCCCGG TTACTGGAAC GTTGTGAGGG TAAACAACTG GCGGTATGGA TGCGGCGGGA CCAGAGAAAA ATCACTCAGG GTCAATGCCA GCGCTTCGTT AATACAGATG TAGGTGTTCC ACAGGGTAGC CAGCAGCATC CTGCGATGCA GATCCGGAAC ATAATGGTGC AGGGCGCTGA CTTCCGCGTT TCCAGACTTT ACGAAACACG GAAACCGAAG ACCATTCATG TTGTTGCTCA GGTCGCAGAC GTTTTGCAGC AGCAGTCGCT TCACGTTCGC TCGCGTATCG GTGATTCATT CTGCTAACCA GTAAGGCAAC CCCGCCAGCC TAGCCGGGTC CTCAACGACA GGAGCACGAT CATGCGCACC CGTGGCCAGG ACCCAACGCT GCCCGAGATG CGCCGCGTGC GGCTGCTGGA GATGGCGGAC GCGATGGATA TGTTCTGCCA AGGGTTGGTT TGCGCATTCA CAGTTCTCCG CAAGAATTGA TTGGCTCCAA TTCTTGGAGT GGTGAATCCG TTAGCGAGGT GCCGCCGGCT TCCATTCAGG TCGAGGTGGC CCGGCTCCAT GCACCGCGAC GCAACGCGGG GAGGCAGACA AGGTATAGGG CGGCGCCTAC AATCCATGCC AACCCGTTCC ATGTGCTCGC CGAGGCGGCA TAAATCGCCG TGACGATCAG CGGTCCAGTG ATCGAAGTTA GGCTGGTAAG AGCCGCGAGC GATCCTTGAA GCTGTCCCTG ATGGTCGTCA TCTACCTGCC TGGACAGCAT GGCCTGCAAC GCGGGCATCC CGATGCCGCC GGAAGCGAGA AGAATCATAA TGGGGAAGGC CATCCAGCCT CGCGTCGCGA ACGCCAGCAA GACGTAGCCC AGCGCGTCGG CCGCCATGCC GGCGATAATG GCCTGCTTCT CGCCGAAACG TTTGGTGGCG GGACCAGTGA CGAAGGCTTG AGCGAGGGCG TGCAAGATTC CGAATACCGC AAGCGACAGG CCGATCATCG TCGCGCTCCA GCGAAAGCGG TCCTCGCCGA AAATGACCCA GAGCGCTGCC GGCACCTGTC CTACGAGTTG CATGATAAAG AAGACAGTCA TAAGTGCGGC GACGATAGTC ATGCCCCGCG CCCACCGGAA GGAGCTGACT GGGTTGAAGG CTCTCAAGGG CATCGGTCGA CGCTCTCCCT TATGCGACTC CTGCATTAGG AAGCAGCCCA GTAGTAGGTT GAGGCCGTTG AGCACCGCCG CCGCAAGGAA TGGTGCATGC AAGGAGATGG CGCCCAACAG TCCCCCGGCC ACGGGGCCTG CCACCATACC CACGCCGAAA CAAGCGCTCA TGAGCCCGAA GTGGCGAGCC CGATCTTCCC CATCGGTGAT GTCGGCGATA TAGGCGCCAG CAACCGCACC TGTGGCGCCG GTGATGCCGG CCACGATGCG TCCGGCGTAG AGGATCGAGA TCTCGATCCC GCGAAAT