MyleA.18251.a
CONSERVED HYPOTHETICAL PROTEIN
| CENTER ID: | MyleA.18251.a |
| ORGANISM: | Mycobacterium leprae Br4923 |
| ASSOCIATED DISEASE: | Hansen's disease, leprosy |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIC |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MyleA.18251.a.A1.GE34373 | full length | 1 | 207 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MyleA.18251.a.A1.PS01435 | full length | 1 | 207 |
Structures
4WKW
DEPOSITED: 10/3/2014
DETERMINATION: XRay
CLONE: MyleA.18251.a.A1.GE34373
PROTEIN: MyleA.18251.a.A1.PS01435
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_237696 |
| PATRIC ID: | fig|561304.4.peg.2788 |
| RefSeq: | YP_002503674.1 |
| UniProt: | A0A0H3MQY4 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MPEKASVKYQ ADLWFDPLCP WCWITSRWIL EVEKVRDVEV HFHVMSLAIL NENREDLTEN YLENITKAWG PARVVIAAEQ ANGASVLDPL YTAMGIRIHN EDNKNLDEVI KRSLADTGLP AELAAAAQSN AYDDALRESH HAGMDPVGDD VGTPTVHVNG VAFFGPVLSK IPRGEEAGKL WDASLTLAAY PHFFELKRTR TEPPQFA
NT Sequence
atgcccgaga aggcgtctgt caagtatcaa gccgatttat ggttcgaccc gctatgcccg tggtgctgga tcacttcgcg ctggatcctt gaggtggaga aggttcgcga tgtcgaggtg cactttcacg tgatgagtct ggcgatactt aacgaaaacc gcgaagatct gacggaaaac tatctcgaaa atataacgaa ggcatgggga ccggcgcggg tggtgatcgc tgctgaacag gctaatggtg ccagtgtgct ggaccccttg tacactgcga tgggtatccg gattcacaac gaggacaaca aaaacctcga cgaggtcatc aagagatcgc ttgccgacac tggtctgcca gcggagctgg ccgcggcagc tcaaagtaac gcctacgacg acgcgctgcg tgagagtcac cacgccggca tggacccggt tggcgacgat gtcggcacac cgactgtgca cgtcaacggt gtggcgttct tcgggccggt attgtcgaaa atcccgcgcg gtgaggaggc cggcaagctc tgggacgcct cgctgacttt ggcggcttac ccacactttt ttgagctcaa gcggacccga accgaaccgc cgcagttcgc ctag
Details for MyleA.18251.a.A1.GE34373
| HARVESTED ON: | 1/10/2012 |
| SEQUENCED ON: | 1/13/2012 |
| EXPECTED MW: | 25kDa |
| OBSERVED MW: | 27kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | Low Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMPEKASVKY QADLWFDPLC PWCWITSRWI LEVEKVRDVE VHFHVMSLAI LNENREDLTE NYLENITKAW GPARVVIAAE QANGASVLDP LYTAMGIRIH NEDNKNLDEV IKRSLADTGL PAELAAAAQS NAYDDALRES HHAGMDPVGD DVGTPTVHVN GVAFFGPVLS KIPRGEEAGK LWDASLTLAA YPHFFELKRT RTEPPQFA
Validated NT Sequence
ttattaggcg aactgcggcg gttcggttcg ggtccgcttg agctcaaaaa agtgtgggta agccgccaaa gtcagcgagg cgtcccagag cttgccggcc tcctcaccgc gcgggatttt cgacaatacc ggcccgaaga acgccacacc gttgacgtgc acagtcggtg tgccgacatc gtcgccaacc gggtccatgc cggcgtggtg actctcacgc agcgcgtcgt cgtaggcgtt actttgagct gccgcggcca gctccgctgg cagaccagtg tcggcaagcg atctcttgat gacctcgtcg aggtttttgt tgtcctcgtt gtgaatccgg atacccatcg cagtgtacaa ggggtccagc acactggcac cattagcctg ttcagcagcg atcaccaccc gcgccggtcc ccatgccttc gttatatttt cgagatagtt ttccgtcaga tcttcgcggt tttcgttaag tatcgccaga ctcatcacgt gaaagtgcac ctcgacatcg cgaaccttct ccacctcaag gatccagcgc gaagtgatcc agcaccacgg gcatagcggg tcgaaccata aatcggcttg atacttgaca gacgccttct cgggcatcga accaggaccc tgggtctgag cttccagggt acccatatga tggtgatggt gatgagccat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMPEKASVKY QADLWFDPLC PWCWITSRWI LEVEKVRDVE VHFHVMSLAI LNENREDLTE NYLENITKAW GPARVVIAAE QANGASVLDP LYTAMGIRIH NEDNKNLDEV IKRSLADTGL PAELAAAAQS NAYDDALRES HHAGMDPVGD DVGTPTVHVN GVAFFGPVLS KIPRGEEAGK LWDASLTLAA YPHFFELKRT RTEPPQFA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcccgagaag gcgtctgtca agtatcaagc cgatttatgg ttcgacccgc tatgcccgtg gtgctggatc acttcgcgct ggatccttga ggtggagaag gttcgcgatg tcgaggtgca ctttcacgtg atgagtctgg cgatacttaa cgaaaaccgc gaagatctga cggaaaacta tctcgaaaat ataacgaagg catggggacc ggcgcgggtg gtgatcgctg ctgaacaggc taatggtgcc agtgtgctgg accccttgta cactgcgatg ggtatccgga ttcacaacga ggacaacaaa aacctcgacg aggtcatcaa gagatcgctt gccgacactg gtctgccagc ggagctggcc gcggcagctc aaagtaacgc ctacgacgac gcgctgcgtg agagtcacca cgccggcatg gacccggttg gcgacgatgt cggcacaccg actgtgcacg tcaacggtgt ggcgttcttc gggccggtat tgtcgaaaat cccgcgcggt gaggaggccg gcaagctctg ggacgcctcg ctgactttgg cggcttaccc acactttttt gagctcaagc ggacccgaac cgaaccgccg cagttcgcct aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa
Details for MyleA.18251.a.A1.PS01435
| PURIFICATION DATe: | 3/28/2012 |
| CONCENTRATION: | 69.4mg/ml |
| OBSERVED MW: | 28kDa |
| EXPRESSION LEVEL: | Low Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 6 |
| VIAL VOLUME: | 110µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMPEKASVKY QADLWFDPLC PWCWITSRWI LEVEKVRDVE VHFHVMSLAI LNENREDLTE NYLENITKAW GPARVVIAAE QANGASVLDP LYTAMGIRIH NEDNKNLDEV IKRSLADTGL PAELAAAAQS NAYDDALRES HHAGMDPVGD DVGTPTVHVN GVAFFGPVLS KIPRGEEAGK LWDASLTLAA YPHFFELKRT RTEPPQFA
Validated NT Sequence
ttattaggcg aactgcggcg gttcggttcg ggtccgcttg agctcaaaaa agtgtgggta agccgccaaa gtcagcgagg cgtcccagag cttgccggcc tcctcaccgc gcgggatttt cgacaatacc ggcccgaaga acgccacacc gttgacgtgc acagtcggtg tgccgacatc gtcgccaacc gggtccatgc cggcgtggtg actctcacgc agcgcgtcgt cgtaggcgtt actttgagct gccgcggcca gctccgctgg cagaccagtg tcggcaagcg atctcttgat gacctcgtcg aggtttttgt tgtcctcgtt gtgaatccgg atacccatcg cagtgtacaa ggggtccagc acactggcac cattagcctg ttcagcagcg atcaccaccc gcgccggtcc ccatgccttc gttatatttt cgagatagtt ttccgtcaga tcttcgcggt tttcgttaag tatcgccaga ctcatcacgt gaaagtgcac ctcgacatcg cgaaccttct ccacctcaag gatccagcgc gaagtgatcc agcaccacgg gcatagcggg tcgaaccata aatcggcttg atacttgaca gacgccttct cgggcatcga accaggaccc tgggtctgag cttccagggt acccatatga tggtgatggt gatgagccat
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMPEKASVKY QADLWFDPLC PWCWITSRWI LEVEKVRDVE VHFHVMSLAI LNENREDLTE NYLENITKAW GPARVVIAAE QANGASVLDP LYTAMGIRIH NEDNKNLDEV IKRSLADTGL PAELAAAAQS NAYDDALRES HHAGMDPVGD DVGTPTVHVN GVAFFGPVLS KIPRGEEAGK LWDASLTLAA YPHFFELKRT RTEPPQFA
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GCCCGAGAAG GCGTCTGTCA AGTATCAAGC CGATTTATGG TTCGACCCGC TATGCCCGTG GTGCTGGATC ACTTCGCGCT GGATCCTTGA GGTGGAGAAG GTTCGCGATG TCGAGGTGCA CTTTCACGTG ATGAGTCTGG CGATACTTAA CGAAAACCGC GAAGATCTGA CGGAAAACTA TCTCGAAAAT ATAACGAAGG CATGGGGACC GGCGCGGGTG GTGATCGCTG CTGAACAGGC TAATGGTGCC AGTGTGCTGG ACCCCTTGTA CACTGCGATG GGTATCCGGA TTCACAACGA GGACAACAAA AACCTCGACG AGGTCATCAA GAGATCGCTT GCCGACACTG GTCTGCCAGC GGAGCTGGCC GCGGCAGCTC AAAGTAACGC CTACGACGAC GCGCTGCGTG AGAGTCACCA CGCCGGCATG GACCCGGTTG GCGACGATGT CGGCACACCG ACTGTGCACG TCAACGGTGT GGCGTTCTTC GGGCCGGTAT TGTCGAAAAT CCCGCGCGGT GAGGAGGCCG GCAAGCTCTG GGACGCCTCG CTGACTTTGG CGGCTTACCC ACACTTTTTT GAGCTCAAGC GGACCCGAAC CGAACCGCCG CAGTTCGCCT AAACAGCACG AACAAGTTCT GCAGCCAAGC TTCTCGAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AA