MyleA.01382.a
INORGANIC PYROPHOSPHATASE PPA (PYROPHOSPHATE PHOSPHO-HYDROLASE) (PPASE) (INORGANIC DIPHOSPHATASE) (DIPHOSPHATE PHOSPHO-HYDROLASE
| CENTER ID: | MyleA.01382.a |
| ORGANISM: | Mycobacterium leprae Br4923 |
| ASSOCIATED DISEASE: | Hansen's disease, leprosy |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIC |
Ordering Clones & Proteins
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MyleA.01382.a.A1.GE34134 | full length | 1 | 162 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Structures
4ECP
DEPOSITED: 3/26/2012
DETERMINATION: XRay
CLONE: MyleA.01382.a.A1.GE34134
PROTEIN: MyleA.01382.a.A1.PW34776
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_128875 |
| PATRIC ID: | fig|561304.4.peg.332 |
| RefSeq: | YP_002502907.1 |
| UniProt: | A0A0H3MP66 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MQFDVTIEIP KGQRNKYEVD HKTGRVRLDR YLYTPMAYPT DYGFIEDTLG EDGDPLDALV LLPEPLFPGV LVEARPVGMF RMVDEHGGDD KVLCVPVNDH RWDHIHGIID VPTFELDAIK HFFVHYKDLE PGKFVKAADW VGRDEAEAEV QRSVERFKAG GH
NT Sequence
gtgcaattcg acgtgaccat cgaaattccg aaaggccagc ggaacaagta cgaggtcgac cacaaaacgg ggcgggtgcg tctagaccgc tacctttaca ccccgatggc ctatccgaca gactacggct tcattgaaga caccctcggc gaagacggcg acccgctgga tgcactggtg ctgctaccgg agccgctgtt ccctggcgta ctagtggagg cacgtccagt aggtatgttc cggatggtcg acgagcacgg cggcgacgac aaggtgttgt gcgttccagt caacgaccac cgatgggatc acatccacgg catcatcgat gttccgacct tcgagctgga cgcgatcaag catttcttcg tacactacaa ggatctagag ccaggcaagt tcgtgaaagc cgcagactgg gtaggccgag acgaagctga agcagaagtg cagcgttcag tggaacggtt caaggctggc gggcactga
Details for MyleA.01382.a.A1.GE34134
| HARVESTED ON: | 12/6/2011 |
| SEQUENCED ON: | 12/8/2011 |
| EXPECTED MW: | 20kDa |
| OBSERVED MW: | 20kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Many (50-100) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVQFDVTIE IPKGQRNKYE VDHKTGRVRL DRYLYTPMAY PTDYGFIEDT LGEDGDPLDA LVLLPEPLFP GVLVEARPVG MFRMVDEHGG DDKVLCVPVN DHRWDHIHGI IDVPTFELDA IKHFFVHYKD LEPGKFVKAA DWVGRDEAEA EVQRSVERFK AGGH
Validated NT Sequence
ttattagtgc ccgccagcct tgaaccgttc cactgaacgc tgcacttctg cttcagcttc gtctcggcct acccagtctg cggctttcac gaacttgcct ggctctagat ccttgtagtg tacgaagaaa tgcttgatcg cgtccagctc gaaggtcgga acatcgatga tgccgtggat gtgatcccat cggtggtcgt tgactggaac gcacaacacc ttgtcgtcgc cgccgtgctc gtcgaccatc cggaacatac ctactggacg tgcctccact agtacgccag ggaacagcgg ctccggtagc agcaccagtg catccagcgg gtcgccgtct tcgccgaggg tgtcttcaat gaagccgtag tctgtcggat aggccatcgg ggtgtaaagg tagcggtcta gacgcacccg ccccgttttg tggtcgacct cgtacttgtt ccgctggcct ttcggaattt cgatggtcac gtcgaattgc accatcgaac caggaccctg ggtctgagct tccagggtac ccatatgatg gtgatggtga tgagccat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVQFDVTIE IPKGQRNKYE VDHKTGRVRL DRYLYTPMAY PTDYGFIEDT LGEDGDPLDA LVLLPEPLFP GVLVEARPVG MFRMVDEHGG DDKVLCVPVN DHRWDHIHGI IDVPTFELDA IKHFFVHYKD LEPGKFVKAA DWVGRDEAEA EVQRSVERFK AGGH
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgcaattc gacgtgacca tcgaaattcc gaaaggccag cggaacaagt acgaggtcga ccacaaaacg gggcgggtgc gtctagaccg ctacctttac accccgatgg cctatccgac agactacggc ttcattgaag acaccctcgg cgaagacggc gacccgctgg atgcactggt gctgctaccg gagccgctgt tccctggcgt actagtggag gcacgtccag taggtatgtt ccggatggtc gacgagcacg gcggcgacga caaggtgttg tgcgttccag tcaacgacca ccgatgggat cacatccacg gcatcatcga tgttccgacc ttcgagctgg acgcgatcaa gcatttcttc gtacactaca aggatctaga gccaggcaag ttcgtgaaag ccgcagactg ggtaggccga gacgaagctg aagcagaagt gcagcgttca gtggaacggt tcaaggctgg cgggcactaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa