SSGCID: Target Page

WoovA.18708.a

isoleucyl-tRNA synthetase

CENTER ID:	WoovA.18708.a
ORGANISM:	Wolbachia endosymbiont of Onchocerca volvulus
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	NA
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
WoovA.18708.a.EW11.GE00528	full length		1	1092

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MKHYPGTTSS PNFSLLENAI IKFWQENKIF ERSVEERSKD NCFVFYDGPP FANGLPHYGH LLTGFIKDAF AKYQTMLQKR VERRFGWDCH GLPAEMGAEK ELGISGRTEV EKFGIEKFNN YCRASVMKFS LKWKKYINRQ ARWVDFYNGY KTMDKSFMES VMWAFKQLYD RGLVYESVRV VPYSWACETP LSNFETRLDN SYKERTSKAV TVAFELLENP QQLKQKCKLL AWTTTPWTLP SNLALAIGKD IEYCAVLVRS LVSFQDVTLE STSEFQCRGA KTMEDTGMTM KSNKGNLVSD EIYIFAKSYL ERFIDYCEKN NITYKDCNIR LKANDLVGLS YKPLFDYFKG TRNAFRIFIA DYVIGGDGTG VVHTAPGFGE EDFYLCHGHN IPAVCPIDDS GKFTVEVPDL AGVHVFDASD MVIRKLKEQG NWFETEQYIH NYPHCWRTDT PLIYRAMPSW YIAVTKFKRR MMELNKRVNW IPNHIRDGQF GKWLEGAHDW SISRNRFWGT PIPVWKSDDA RYPRVDVYGS IAELERDFNV KIDDLHRPFI DSLTRPNPDD PTKKSVMRRI SDVFDCWFES GSMPFAQVHY PFENKKWFRD NFPADFITEY IAQTRGWFYT LFVLSTALFD SEPFKNCICH GVVLDIKGQK LSKRLNNYVD PMEVFDRYGS DALRFLMLSN SIVCGGDLLL DKEGNLIRDV LRNVIKPIWS SYHFFTMYAN ADEIKAEVCK DYQSTIDCYM ISKCFEAIES IQISMNNYNS QGACKVLIDF FEVLNNWYIR RNRRRFWKSG LDQNKIDAYN VLYTVFYYIL KAAAPLLPFI TETIWQGLKY KETSVHLADF PQLEKYDSEF IIKMDLVREV CNAALSIRNT FNMRIRQPLS SMTIYHQFFC GFFEDNYQEM IKDEVNIKSL ELVNRLEDIA SLELKLNFPL LGKKIPNKIK KLVQYVKEGK WRQIGDEQVF LGDKLENYVI GKGEYELLLK VNRKYSAVFD NNKGVVVLNT ELNDELILEG LARDVVRLIQ ETRKQAGFHI SDRIRVIVKT KDEGIKKAVD TWSGYIKEQT LASSLEVSVE IEADFYFKEY QNLIIGVRLN Y

NT Sequence

atgaagcatt atcctggtac aactagtagt cctaattttt ctttattaga aaacgcaatc ataaaatttt ggcaggagaa caaaattttt gagagatcgg ttgaagaacg ttctaaagat aattgttttg ttttttatga tggacctcca tttgcaaatg ggctccctca ttatggacat ttacttactg gttttataaa ggatgcattt gcaaaatatc aaactatgct acaaaagaga gttgaacgta gatttggttg ggattgccat gggctaccag ctgaaatggg tgcagaaaag gaacttggaa tatccggcag aactgaagtg gaaaaattcg gtattgagaa attcaataac tattgtcgtg cttctgtgat gaaattttcc ttaaaatgga agaagtatat aaataggcaa gcaaggtggg tagattttta taatggctat aaaaccatgg ataaatcgtt tatggagtca gttatgtggg cgtttaagca gctttatgat agaggtttag tgtatgaatc agtacgcgtt gttccatata gctgggcatg tgaaactcca ttatctaatt ttgaaacaag acttgataac tcttacaaag aaaggactag caaggctgtc actgttgcat ttgagctttt ggagaatcca cagcaattga agcaaaaatg taagttgctt gcttggacta caactccttg gacgttacca agtaacttag ctttagcaat agggaaagat attgaatatt gtgcggtatt agttcgttcc ttagtgtcat tccaggatgt gacactggaa tctacttctg aatttcagtg tcgaggtgct aagactatgg aagacactgg aatgacaatg aagagtaata aaggaaattt agtgagtgat gagatatata tctttgctaa aagctactta gaaagattta tcgattactg tgaaaaaaat aatattacat ataaagactg taatataagg cttaaagcaa atgatcttgt aggtctttct tataaaccac tgtttgatta ctttaagggt acaagaaatg cgtttcgcat ttttattgct gattatgtta taggaggaga tggtactggt gttgtgcaca ctgctcctgg atttggtgaa gaagactttt acctttgcca tggtcataat attccagccg tttgtccaat tgatgatagt ggtaaattta ctgttgaagt tccagattta gctggagttc atgtttttga tgccagcgat atggtaataa ggaaattgaa ggaacaagga aactggttcg aaactgagca atatattcac aattatccac actgctggag aactgatact cctttaatct atcgtgctat gccctcttgg tatattgctg tgacaaagtt taagaggagg atgatggagc taaacaaaag agttaattgg ataccaaatc atataagaga tggtcaattt ggaaaatggc ttgaaggagc acatgattgg tcaatttcac gtaatcggtt ttggggtaca ccaattcctg tatggaaatc ggatgatgca aggtatccaa gagtggatgt gtatggttca atagcagaac tggagcgaga ttttaatgtt aaaatagatg atttgcacag accatttatc gattctttaa caagaccaaa tcctgatgat ccaacgaaaa aatcagttat gcgccgtata tccgacgtgt ttgactgctg gtttgagtct ggctcaatgc cgtttgcaca agtccactat ccgtttgaaa ataagaaatg gtttagagat aatttccctg cagattttat aactgaatat atagcgcaaa caagagggtg gttctatacg ctttttgtgt tgtccactgc tttgtttgac agtgaaccat ttaagaattg catatgtcac ggtgtagttc tggatataaa aggacagaaa ttatctaagc gtttaaataa ttatgtagac ccaatggagg tttttgatag gtatggttct gatgcgctgc gctttcttat gctgtctaat tccattgtct gtggtggtga tttactactt gataaagaag gaaacttgat acgagatgtc ttaaggaacg tgataaaacc tatttggagt agttatcact tctttactat gtatgcgaat gcagatgaaa ttaaagctga agtttgtaaa gattatcaaa gtactatcga ttgttacatg atctctaagt gttttgaagc tatagaaagc atccaaattt ctatgaacaa ctataattct caaggagctt gcaaagttct gatagatttt tttgaagtgc taaataactg gtatattcgt cgcaatcgta ggcgtttttg gaaaagtggt ctagatcaga acaaaattga tgcttataat gtcttatata cggtttttta ttatatatta aaagccgcgg ctcctttatt gccgtttata acagagacta tatggcaggg gcttaagtat aaagaaacat ctgttcattt agctgatttt ccacagttag aaaagtatga tagtgagttt attatcaaaa tggatttggt aagagaggtg tgcaatgctg cactttctat cagaaatact tttaatatgc gtatcaggca accacttagc agcatgacaa tttatcatca atttttttgt ggtttttttg aagataatta tcaggagatg ataaaggatg aggtgaatat aaaaagctta gaattagtaa acaggcttga agatattgca tcattagagt taaaactaaa tttcccacta cttggaaaga agatcccaaa taaaatcaag aagctagttc aatatgttaa agagggaaaa tggaggcaga ttggagatga acaagtattt ttaggggata agttagaaaa ctatgttata ggaaaaggtg agtatgaact attactaaaa gtaaatagaa aatattctgc tgtatttgat aacaacaaag gggttgttgt cttaaacact gaactaaatg atgaattaat tctagaagga cttgcgagag atgtagtgag gcttattcag gaaactagaa aacaagctgg ttttcatata tccgatagaa ttagagtaat agtcaaaaca aaggatgagg gaattaaaaa agcggtggat acatggagtg ggtatataaa agagcaaact cttgcctcat ctttagaggt tagtgtagaa atagaagccg atttctattt taaggaatat caaaatttaa taattggtgt cagattaaat tattaa

Details for WoovA.18708.a.EW11.GE00528

HARVESTED ON:	12/17/2015
SEQUENCED ON:	5/4/2017
EXPECTED MW:	138kDa
OBSERVED MW:	138kDa
ANTIBIOTIC MARKER:	kanamycin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MGHHHHHHSG EVKPEVKPET HINLKVSDGS SEIFFKIKKT TPLRRLMEAF AKRQGKEMDS LRFLYDGIRI QADQTPEDLD MEDNDIIEAH REQIGGMKHY PGTTSSPNFS LLENAIIKFW QENKIFERSV EERSKDNCFV FYDGPPFANG LPHYGHLLTG FIKDAFAKYQ TMLQKRVERR FGWDCHGLPA EMGAEKELGI SGRTEVEKFG IEKFNNYCRA SVMKFSLKWK KYINRQARWV DFYNGYKTMD KSFMESVMWA FKQLYDRGLV YESVRVVPYS WACETPLSNF ETRLDNSYKE RTSKAVTVAF ELLENPQQLK QKCKLLAWTT TPWTLPSNLA LAIGKDIEYC AVLVRSLVSF QDVTLESTSE FQCRGAKTME DTGMTMKSNK GNLVSDEIYI FAKSYLERFI DYCEKNNITY KDCNIRLKAN DLVGLSYKPL FDYFKGTRNA FRIFIADYVI GGDGTGVVHT APGFGEEDFY LCHGHNIPAV CPIDDSGKFT VEVPDLAGVH VFDASDMVIR KLKEQGNWFE TEQYIHNYPH CWRTDTPLIY RAMPSWYIAV TKFKRRMMEL NKRVNWIPNH IRDGQFGKWL EGAHDWSISR NRFWGTPIPV WKSDDARYPR VDVYGSIAEL ERDFNVKIDD LHRPFIDSLT RPNPDDPTKK SVMRRISDVF DCWFESGSMP FAQVHYPFEN KKWFRDNFPA DFITEYIAQT RGWFYTLFVL STALFDSEPF KNCICHGVVL DIKGQKLSKR LNNYVDPMEV FDRYGSDALR FLMLSNSIVC GGDLLLDKEG NLIRDVLRNV IKPIWSSYHF FTMYANADEI KAEVCKDYQS TIDCYMISKC FEAIESIQIS MNNYNSQGAC KVLIDFFEVL NNWYIRRNRR RFWKSGLDQN KIDAYNVLYT VFYYILKAAA PLLPFITETI WQGLKYKETS VHLADFPQLE KYDSEFIIKM DLVREVCNAA LSIRNTFNMR IRQPLSSMTI YHQFFCGFFE DNYQEMIKDE VNIKSLELVN RLEDIASLEL KLNFPLLGKK IPNKIKKLVQ YVKEGKWRQI GDEQVFLGDK LENYVIGKGE YELLLKVNRK YSAVFDNNKG VVVLNTELND ELILEGLARD VVRLIQETRK QAGFHISDRI RVIVKTKDEG IKKAVDTWSG YIKEQTLASS LEVSVEIEAD FYFKEYQNLI IGVRLNY

Full NT Sequence (Expression Vector + Insert)

tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg aattaattct tagaaaaact catcgagcat caaatgaaac tgcaatttat tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat gaaggagaaa actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg attccgactc gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta tcaagtgaga aatcaccatg agtgacgact gaatccggtg agaatggcaa aagtttatgc atttctttcc agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca tcaaccaaac cgttattcat tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca tcaacaatat tttcacctga atcaggatat tcttctaata cctggaatgc tgttttcccg gggatcgcag tggtgagtaa ccatgcatca tcaggagtac ggataaaatg cttgatggtc ggaagaggca taaattccgt cagccagttt agtctgacca tctcatctgt aacatcattg gcaacgctac ctttgccatg tttcagaaac aactctggcg catcgggctt cccatacaat cgatagattg tcgcacctga ttgcccgaca ttatcgcgag cccatttata cccatataaa tcagcatcca tgttggaatt taatcgcggc ctagagcaag acgtttcccg ttgaatatgg ctcataacac cccttgtatt actgtttatg taagcagaca gttttattgt tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgagatc ccggtgccta atgagtgagc taacttacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgccag ggtggttttt cttttcacca gtgagacggg caacagctga ttgcccttca ccgcctggcc ctgagagagt tgcagcaagc ggtccacgct ggtttgcccc agcaggcgaa aatcctgttt gatggtggtt aacggcggga tataacatga gctgtcttcg gtatcgtcgt atcccactac cgagatatcc gcaccaacgc gcagcccgga ctcggtaatg gcgcgcattg cgcccagcgc catctgatcg ttggcaacca gcatcgcagt gggaacgatg ccctcattca gcatttgcat ggtttgttga aaaccggaca tggcactcca gtcgccttcc cgttccgcta tcggctgaat ttgattgcga gtgagatatt tatgccagcc agccagacgc agacgcgccg agacagaact taatgggccc gctaacagcg cgatttgctg gtgacccaat gcgaccagat gctccacgcc cagtcgcgta ccgtcttcat gggagaaaat aatactgttg atgggtgtct ggtcagagac atcaagaaat aacgccggaa cattagtgca ggcagcttcc acagcaatgg catcctggtc atccagcgga tagttaatga tcagcccact gacgcgttgc gcgagaagat tgtgcaccgc cgctttacag gcttcgacgc cgcttcgttc taccatcgac accaccacgc tggcacccag ttgatcggcg cgagatttaa tcgccgcgac aatttgcgac ggcgcgtgca gggccagact ggaggtggca acgccaatca gcaacgactg tttgcccgcc agttgttgtg ccacgcggtt gggaatgtaa ttcagctccg ccatcgccgc ttccactttt tcccgcgttt tcgcagaaac gtggctggcc tggttcacca cgcgggaaac ggtctgataa gagacaccgg catactctgc gacatcgtat aacgttactg gtttcacatt caccaccctg aattgactct cttccgggcg ctatcatgcc ataccgcgaa aggttttgcg ccattcgatg gtgtccggga tctcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aattaatacg actcactata ggggaattgt gagcggataa caattcccct ctagaaataa ttttgtttaa ctttaagaag gagatatacc atgggccacc accaccacca ccatagcggc gaagtaaaac cggaagtgaa gccggaaacc cacatcaacc tgaaggttag cgacggtagc agcgaaatct tcttcaagat taagaaaacc accccgctgc gtcgcctgat ggaagcattc gctaaacgcc agggcaagga aatggattcc ctgcgctttc tgtacgacgg tatccgtatt caggcagacc agactccgga agatctggac atggaagata acgacattat cgaagcacac cgtgaacaaa tcggtggaat gaaacactat ccaggtacta cctcttcccc taatttctcg ttgctggaga atgcaatcat taagttctgg caggaaaaca aaatctttga acgcagcgtt gaggaacgtt ccaaggacaa ctgcttcgtg ttctacgatg gtcctccgtt tgcgaatggc ctgccgcatt acggtcatct gctgaccggt tttatcaaag acgcctttgc gaaataccag accatgctgc aaaagcgtgt cgagcgtcgt tttggttggg attgtcacgg cctgccggct gaaatgggtg cggagaaaga gctgggtatt tctggccgca ccgaggttga gaaatttggt atcgaaaagt tcaacaatta ctgtcgtgca agcgtgatga aattcagctt gaagtggaag aaatacatca atcgtcaggc acgctgggtt gatttctata atggttacaa gacgatggac aagtccttca tggagtctgt tatgtgggcg ttcaaacaac tgtacgaccg tggtctggta tatgagagcg tccgtgtggt cccgtacagc tgggcgtgcg aaaccccgct gagcaatttc gaaacccgtc tggataatag ctacaaagag cgtacctcca aggccgttac cgtggcgttc gagctgctgg agaacccgca gcagctgaag caaaagtgta agctgctggc atggaccacc acgccgtgga ccctgccgag caatctggcg ctggcgattg gtaaagatat tgagtactgt gccgtcttgg tgcgtagcct ggtcagcttt caagacgtga cgctggagag cacgagcgag ttccagtgcc gtggtgcaaa aactatggaa gatacgggta tgaccatgaa aagcaacaaa ggcaatctgg tgtccgatga aatctatatc tttgctaagt cttatttgga acgtttcatt gactattgcg agaagaacaa catcacctac aaggattgca acatccgttt gaaagcgaac gacctggtcg gcctgagcta caagccgctg tttgactact tcaagggtac gcgcaatgcg tttcgcattt tcatcgcgga ttacgtgatt ggtggcgatg gtaccggtgt tgtgcacacg gcgccaggct ttggtgaaga ggatttctat ctgtgccacg gccataacat tccggcagtg tgtccgatcg acgacagcgg taagtttact gttgaagtgc cggatctggc tggtgtccac gtgtttgacg cgagcgacat ggtgatccgt aagttaaaag aacagggtaa ttggtttgaa accgagcaat acattcacaa ctacccgcac tgctggcgca ccgacacccc gctcatttac cgcgcgatgc cgtcgtggta tattgcagtt accaagttca agcgtcgtat gatggaattg aataaacgcg ttaattggat tccgaatcac attcgtgatg gccagtttgg caagtggctt gagggcgccc acgattggag cattagccgt aatcgtttct ggggtacgcc gattccggtg tggaaaagcg acgacgcgcg ttatccgcgt gttgacgtct atggtagcat cgctgagctg gagcgcgact tcaacgtcaa gatcgatgat ttgcaccgtc cgttcattga ttccctgacg cgtccgaatc cagatgaccc gacgaagaaa agcgtgatgc gccgtattag cgacgtcttt gattgctggt tcgagtcggg cagcatgccg tttgcgcaag tgcattaccc gtttgagaat aagaaatggt ttcgtgacaa ttttccagcg gatttcatta ccgagtatat cgcccaaacg cgtggttggt tctatacgct gtttgtcctg agcacggcgt tgttcgatag cgagccgttc aagaactgta tttgtcatgg tgtcgttctg gacattaagg gtcagaaact gagcaaacgc ctgaataact acgtcgatcc gatggaggtc tttgaccgct atggtagcga cgcactgcgt ttcctgatgc tgagcaactc aattgtttgc ggtggcgact tgttgctgga caaagagggt aatctgatcc gtgacgtcct gcgcaacgtg attaagccga tttggtctag ctaccacttt ttcaccatgt atgcgaacgc cgacgaaatc aaagccgaag tgtgcaagga ctatcaaagc accatcgatt gttatatgat cagcaaatgc tttgaagcga tcgagagcat ccagatcagc atgaacaact acaatagcca gggcgcatgc aaggttctga ttgacttctt tgaggtcctg aacaactggt acatccgccg caatcgccgt cgtttctgga aatccggtct ggatcaaaac aagatcgacg cctataacgt tctgtacacg gttttctact atatcctgaa agctgctgct ccgctgttgc ctttcatcac cgaaacgatt tggcagggct tgaagtacaa agaaaccagc gtgcatctgg ccgacttccc gcaactggag aaatacgata gcgagttcat tatcaaaatg gatttggtcc gcgaagtctg caatgcagcg ctgtctattc gtaatacttt caatatgcgt atccgtcaac cgttgagctc catgaccatc tatcatcagt ttttctgtgg tttcttcgaa gataactacc aggaaatgat caaagacgag gttaacatca agagcctgga actggtcaac cgcctggaag atatcgcatc cctggaactg aagctgaact tcccgctgct gggtaaaaag attccgaaca agattaagaa actggtgcaa tacgttaaag agggcaaatg gcgccagatc ggcgatgagc aagtttttct gggtgataaa ctggagaact acgtgatcgg caaaggtgaa tatgaactgt tactgaaagt gaatcgtaag tatagcgcag tttttgacaa taacaaaggt gtcgttgtcc tgaatactga gctgaacgac gagctgatcc tggagggcct ggcgcgtgac gttgttcgtc tgattcagga aacccgcaaa caagccggct ttcatatttc cgatcgtatt cgtgtcattg ttaagaccaa agacgagggc attaagaaag ccgtggacac ctggagcggc tatatcaaag agcagacgct ggcgagcagc cttgaagtga gcgttgaaat cgaagcggat ttctacttta aagagtacca aaacctgatt atcggtgtgc gcctgaacta ttgataacaa gcttgcggcc gcactcgagc accaccacca ccaccactga gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggat