SSGCID: Target Page

WoovA.17943.a

leucyl-tRNA synthetase

CENTER ID:	WoovA.17943.a
ORGANISM:	Wolbachia endosymbiont of Onchocerca volvulus
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	NA
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
WoovA.17943.a.EW11.GE00527	full length		1	847

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MKYDFKTVEK FYQNKWNFSV SKNSKRDKCY VLEMFPYPSG QIHMGHLRNY TIGDVIARYK RARGFEVLHP IGWDAFGLPA ENAARENNIN PAIWTKENID NMRMQLKSIG LSYNWERELS TCEPDYCKHE QKLFLDLLRH GLAYRKESWV NWDPVDQTVL ANEQVIDGKG WRSGAIVEKR KLTQWFLKIT DFAEDLLKCL QNLKNWPEKV KTMQESWIGK SKGITIEFEI IGLNKKLKIF TIHPHTLFGA SFCAVAAEHP IIKNFKNKKI QDFVDNLKIK GKNDEKIGIY TGLNVKHPFL DRELPLYVAN FILTEYGEGA IFGCPAHDQR DLEFAQKYDL PIIPVICEEG TQNIDSLKET CINDGVMFNS EFLNGLTASK AKEVVIKKLE EKGIGKKTVN YRLHDWGISR QRYWGCPIPI IYCKDCGTIP VPEKDLPVTL PPDVEFTSGG NPLDKHPTWK FVDCPKCGKQ VERETDTFDT FFESSWYFAA FCSEDKFINK DACNRFMPVD YYIGGIEHAI LHLLYSRFFC RALTRCGYFD IKEPFSTLIT QGMVCHTTYK DENGKWLFPE EAKELVALGL KIQVGKVEKM SKSKRNTVDP NFIIRKYGAD TARLFVLSDT PPEKDIEWSD NGIEGCFRYI NKLWRMVMQF KPIAIQNQSP TIQVADTEIK KGNMLSHTEI TPDKNVTNKL LEYRKKMHKL LHGLTDDLET CKLNCAVAKF REMTNLIAEI DIKNGKSLID ESICTLIRVI EPFIPHLAES LWQEIGGEGM LYLQPWPEVA KSLLIDNVVT VAVQINGKLS TIIEVAINLP KEELKKIAMS SVSNKIDQNK VRTIYTVPNK IVNIVV

NT Sequence

atgaaatatg attttaaaac cgttgaaaaa ttttatcaaa ataagtggaa tttctctgta agcaagaata gtaaacgaga taaatgttat gtgttggaaa tgtttccata tccatctggc caaattcata tgggacactt gcgtaactat acaataggag atgtaatagc acgttataag agagcgcgtg gatttgaggt tttgcaccca attggctggg atgcatttgg attaccagct gaaaatgcag caagggagaa caatattaac cccgcaattt ggacaaaaga gaatatagat aatatgcgta tgcaactgaa atctataggt ctttcctata attgggagcg tgaactctcc acgtgtgagc ctgattattg taaacatgag caaaaacttt ttttagatct tttaaggcat gggcttgctt accgaaaaga gtcatgggtt aactgggatc cagtagacca aacagtactt gcgaatgaac aggtaatcga tggaaaaggc tggcgttcag gtgcaattgt tgaaaaacgt aagttaaccc agtggttttt aaagattact gattttgctg aagatttgct caagtgcttg caaaatttaa agaattggcc agaaaaagta aaaacaatgc aagaaagttg gataggaaaa tctaaaggca tcactataga atttgaaata atcggtttaa acaaaaaatt gaaaatcttt acaattcacc ctcacacttt atttggagct tccttttgtg cagtggcagc agagcaccct attataaaga atttcaaaaa caaaaaaata caagattttg tcgataatct gaaaataaag gggaaaaatg acgaaaaaat tggaatttac accggattaa atgtcaaaca tccatttctt gatagggagt tgccactcta tgtagcgaat tttatattga cagaatatgg agaaggtgca atttttgggt gtcctgcaca tgatcagcgt gatcttgaat ttgcacaaaa atatgattta ccaatcattc ctgtaatttg tgaagaagga acacaaaata tagattcttt aaaagaaaca tgtatcaatg atggagtgat gtttaattct gaatttttaa atggattgac ggctagtaaa gcaaaagaag tagtcatcaa aaagcttgaa gaaaaaggaa taggtaaaaa aactgtaaac tatcgcttac atgactgggg aatctcaagg caacgttatt ggggatgccc tatacctatt atatattgta aagattgtgg tactattcca gtaccagaaa aagaccttcc tgtaacttta ccaccggacg tagaatttac aagcggtggc aatccacttg ataaacaccc aacatggaaa tttgttgact gcccaaaatg tggaaaacaa gtggagcgtg aaactgatac atttgacact ttttttgaat cttcttggta ttttgctgca ttttgtagtg aagataagtt tatcaataag gatgcttgca accgttttat gcctgtcgat tattatatag gtggaataga acatgcaatc ttgcacctgc tttactcacg atttttctgc cgcgctttaa ctagatgtgg ttattttgac attaaggaac ctttctctac cttaatcact caaggaatgg tttgtcatac aacttataaa gatgaaaatg gtaaatggct atttcctgaa gaagcaaaag aattagtagc actaggttta aaaatccaag ttggtaaggt cgagaagatg agcaaatcga aaaggaatac agttgatcca aattttatca taagaaaata cggtgcagat actgcccgtt tgtttgtatt gtctgacact cctccagaaa aagacataga atggtcagac aacggtatag aaggctgctt ccgctatata aataaattat ggcgtatggt aatgcaattc aaacctattg ctatccaaaa tcaatcccct accatccaag tagccgacac cgagatcaaa aaaggaaata tgttatcaca tactgaaata acaccagata aaaatgttac aaataaactt ttagagtata gaaagaaaat gcacaaactc ttacacgggc ttacagatga cttagaaacc tgcaaattaa attgtgcagt ggcaaaattc cgtgagatga cgaacttaat agccgaaata gatataaaga acggaaaatc actcattgat gaaagtatat gcaccctaat cagagtaatc gaaccgttta taccacactt agccgaaagc ctatggcaag aaataggggg tgaaggcatg ctatatctac agccttggcc agaagttgct aagtcactac tgattgataa tgtagtaact gtagcagtgc aaatcaacgg gaaattgagt acaataattg aggtagcaat taatctgcct aaagaagaat tgaagaaaat agcaatgagt tctgtatcca ataagataga tcaaaacaaa gttcgaacta tatatactgt accaaacaaa atagtaaaca tagttgtgta a

Details for WoovA.17943.a.EW11.GE00527

HARVESTED ON:	12/17/2015
SEQUENCED ON:	5/4/2017
EXPECTED MW:	108kDa
OBSERVED MW:	108kDa
ANTIBIOTIC MARKER:	kanamycin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MGHHHHHHSG EVKPEVKPET HINLKVSDGS SEIFFKIKKT TPLRRLMEAF AKRQGKEMDS LRFLYDGIRI QADQTPEDLD MEDNDIIEAH REQIGGMKYD FKTVEKFYQN KWNFSVSKNS KRDKCYVLEM FPYPSGQIHM GHLRNYTIGD VIARYKRARG FEVLHPIGWD AFGLPAENAA RENNINPAIW TKENIDNMRM QLKSIGLSYN WERELSTCEP DYCKHEQKLF LDLLRHGLAY RKESWVNWDP VDQTVLANEQ VIDGKGWRSG AIVEKRKLTQ WFLKITDFAE DLLKCLQNLK NWPEKVKTMQ ESWIGKSKGI TIEFEIIGLN KKLKIFTIHP HTLFGASFCA VAAEHPIIKN FKNKKIQDFV DNLKIKGKND EKIGIYTGLN VKHPFLDREL PLYVANFILT EYGEGAIFGC PAHDQRDLEF AQKYDLPIIP VICEEGTQNI DSLKETCIND GVMFNSEFLN GLTASKAKEV VIKKLEEKGI GKKTVNYRLH DWGISRQRYW GCPIPIIYCK DCGTIPVPEK DLPVTLPPDV EFTSGGNPLD KHPTWKFVDC PKCGKQVERE TDTFDTFFES SWYFAAFCSE DKFINKDACN RFMPVDYYIG GIEHAILHLL YSRFFCRALT RCGYFDIKEP FSTLITQGMV CHTTYKDENG KWLFPEEAKE LVALGLKIQV GKVEKMSKSK RNTVDPNFII RKYGADTARL FVLSDTPPEK DIEWSDNGIE GCFRYINKLW RMVMQFKPIA IQNQSPTIQV ADTEIKKGNM LSHTEITPDK NVTNKLLEYR KKMHKLLHGL TDDLETCKLN CAVAKFREMT NLIAEIDIKN GKSLIDESIC TLIRVIEPFI PHLAESLWQE IGGEGMLYLQ PWPEVAKSLL IDNVVTVAVQ INGKLSTIIE VAINLPKEEL KKIAMSSVSN KIDQNKVRTI YTVPNKIVNI VV

Full NT Sequence (Expression Vector + Insert)

tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg aattaattct tagaaaaact catcgagcat caaatgaaac tgcaatttat tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat gaaggagaaa actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg attccgactc gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta tcaagtgaga aatcaccatg agtgacgact gaatccggtg agaatggcaa aagtttatgc atttctttcc agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca tcaaccaaac cgttattcat tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca tcaacaatat tttcacctga atcaggatat tcttctaata cctggaatgc tgttttcccg gggatcgcag tggtgagtaa ccatgcatca tcaggagtac ggataaaatg cttgatggtc ggaagaggca taaattccgt cagccagttt agtctgacca tctcatctgt aacatcattg gcaacgctac ctttgccatg tttcagaaac aactctggcg catcgggctt cccatacaat cgatagattg tcgcacctga ttgcccgaca ttatcgcgag cccatttata cccatataaa tcagcatcca tgttggaatt taatcgcggc ctagagcaag acgtttcccg ttgaatatgg ctcataacac cccttgtatt actgtttatg taagcagaca gttttattgt tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgagatc ccggtgccta atgagtgagc taacttacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgccag ggtggttttt cttttcacca gtgagacggg caacagctga ttgcccttca ccgcctggcc ctgagagagt tgcagcaagc ggtccacgct ggtttgcccc agcaggcgaa aatcctgttt gatggtggtt aacggcggga tataacatga gctgtcttcg gtatcgtcgt atcccactac cgagatatcc gcaccaacgc gcagcccgga ctcggtaatg gcgcgcattg cgcccagcgc catctgatcg ttggcaacca gcatcgcagt gggaacgatg ccctcattca gcatttgcat ggtttgttga aaaccggaca tggcactcca gtcgccttcc cgttccgcta tcggctgaat ttgattgcga gtgagatatt tatgccagcc agccagacgc agacgcgccg agacagaact taatgggccc gctaacagcg cgatttgctg gtgacccaat gcgaccagat gctccacgcc cagtcgcgta ccgtcttcat gggagaaaat aatactgttg atgggtgtct ggtcagagac atcaagaaat aacgccggaa cattagtgca ggcagcttcc acagcaatgg catcctggtc atccagcgga tagttaatga tcagcccact gacgcgttgc gcgagaagat tgtgcaccgc cgctttacag gcttcgacgc cgcttcgttc taccatcgac accaccacgc tggcacccag ttgatcggcg cgagatttaa tcgccgcgac aatttgcgac ggcgcgtgca gggccagact ggaggtggca acgccaatca gcaacgactg tttgcccgcc agttgttgtg ccacgcggtt gggaatgtaa ttcagctccg ccatcgccgc ttccactttt tcccgcgttt tcgcagaaac gtggctggcc tggttcacca cgcgggaaac ggtctgataa gagacaccgg catactctgc gacatcgtat aacgttactg gtttcacatt caccaccctg aattgactct cttccgggcg ctatcatgcc ataccgcgaa aggttttgcg ccattcgatg gtgtccggga tctcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aattaatacg actcactata ggggaattgt gagcggataa caattcccct ctagaaataa ttttgtttaa ctttaagaag gagatatacc atgggccacc accaccacca ccatagcggc gaagtaaaac cggaagtgaa gccggaaacc cacatcaacc tgaaggttag cgacggtagc agcgaaatct tcttcaagat taagaaaacc accccgctgc gtcgcctgat ggaagcattc gctaaacgcc agggcaagga aatggattcc ctgcgctttc tgtacgacgg tatccgtatt caggcagacc agactccgga agatctggac atggaagata acgacattat cgaagcacac cgtgaacaaa tcggtggaat gaaatacgac tttaagactg tggaaaaatt ttaccagaac aaatggaact tcagcgtttc gaagaatagc aaacgtgaca agtgctatgt cctggaaatg tttccgtatc cgagcggcca aattcacatg ggtcatctgc gtaactatac cattggtgat gtcattgcgc gttacaagcg tgcccgcggc tttgaagtcc tgcacccgat cggttgggat gcttttggcc tgccagcgga gaacgcagct cgtgagaaca atatcaaccc ggcaatttgg accaaagaaa acatcgacaa catgcgtatg cagctgaaaa gcattggtct gagctataac tgggagcgtg agctgtctac gtgcgagccg gactactgta aacacgaaca aaagttgttt ctggatctgc tgcgtcatgg cctggcgtac cgcaaggaat cctgggttaa ctgggacccg gttgatcaga ccgttctggc aaacgaacaa gtgattgatg gcaagggttg gcgtagcggt gccatcgtgg agaaacgcaa gctgacgcag tggttcttga aaatcacgga ctttgcagag gatctgctga agtgcttgca gaatctgaag aattggccgg aaaaagtgaa aaccatgcaa gagtcatgga ttggcaagag caagggcatc accattgagt tcgagatcat cggtctgaac aaaaagctga agattttcac gatccatccg cacacgctgt ttggtgccag cttttgcgcg gttgcagcgg aacatccgat cattaagaat ttcaagaaca aaaagatcca agattttgtg gacaacctga agattaaggg taaaaacgac gagaaaattg gtatctacac cggtctgaac gtgaaacacc cgttcttgga ccgcgagctg ccgctgtacg tggcgaattt catcctgacc gagtatggtg agggcgctat cttcggttgt ccggcgcacg atcagcgcga cctggagttt gcacagaaat acgatctgcc gattattccg gtgatctgcg aggagggcac ccagaatatc gactctctga aagaaacctg tattaacgac ggtgtgatgt tcaatagcga gttcttgaat ggcctgacgg cgagcaaagc taaagaagtc gtcatcaaga aattggaaga gaaaggtatc ggcaagaaaa cggtcaacta tagactgcac gattggggta tctcccgcca gcgttactgg ggctgtccga ttccaatcat ttactgtaaa gactgcggta cgattccggt cccggaaaag gatctgccgg ttaccctgcc tcctgacgtc gagttcactt ccggtggcaa tccgttggac aagcacccga cctggaaatt cgttgattgc ccaaagtgcg gtaaacaagt ggaacgtgag actgacacgt tcgatacctt tttcgagagc tcctggtact ttgccgcctt ttgctctgag gataagttta tcaataaaga tgcgtgcaac cgcttcatgc cggtggacta ctatattggt ggcatcgaac acgcgatcct gcatctgctg tattcgcgct tcttttgtcg tgctctgacc cgttgtggtt atttcgacat caaagaaccg tttagcacct tgattaccca aggtatggtt tgtcatacga cctacaaaga tgagaatggc aagtggctgt tcccggaaga agcgaaagaa ttggtcgcgc tgggcctgaa gatccaggtc ggtaaggtag agaagatgag caaaagcaaa cgtaatacgg tcgacccgaa cttcattatc cgtaaatacg gtgcggacac cgctcgtctg ttcgtgctga gcgatactcc gccggagaaa gacatcgaat ggtctgataa cggtatcgag ggttgtttcc gctatatcaa taagttatgg cgtatggtga tgcagtttaa acctatcgcg attcagaatc agagcccgac gattcaagtt gcagataccg agattaagaa aggcaatatg ctgagccaca ctgagatcac cccggacaaa aacgttacca acaagctcct ggagtaccgc aagaaaatgc acaagctgct gcatggtctg accgatgacc tggaaacgtg caagctgaat tgcgccgttg cgaaatttcg tgagatgacg aacctgattg cggaaatcga tatcaaaaac ggtaagagcc tgatcgacga aagcatttgc accctgattc gtgttattga gccgttcatt ccgcacctgg cggagagctt gtggcaagaa attggtggcg agggtatgct gtacttacaa ccgtggcctg aagtggccaa aagcctgctt atcgacaatg ttgttaccgt ggcagtgcag attaacggta agctgagcac gattattgaa gttgcaatca atttgccgaa ggaagaactg aagaaaattg cgatgagcag cgtgtccaat aagattgacc aaaacaaagt gcgtaccatc tataccgttc caaataagat tgtcaatatc gtcgtgtgat aacaagcttg cggccgcact cgagcaccac caccaccacc actgagatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga t