SSGCID: Target Page

WoovA.01187.a

glutamyl-tRNA synthetase

CENTER ID:	WoovA.01187.a
ORGANISM:	Wolbachia endosymbiont of Onchocerca volvulus
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	NA
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
WoovA.01187.a.EW11.GE00526	full length		1	455

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MLSIITRFAP SPTGFLHIGG ARTALFNWLY AKHHGGKFLL RIEDTDKKRS TQKAINAIID GLKWLDVNYD EEIIYQSKRT ERYVEVANLL VKRGKAYYCY CPENKVAEKK AKAREEGKIY KHKCFNVTPN VKPVVRFKVP DSQDIVVNDK IYGQIKVNSK QIDDIVILRS DNTPTYIFAV VIDDHDTRIT DIIRGSDHLT NTFKQLLIYQ ALDFDIPRFA HVPLIHQENG NKLSKRHGAT SICDYKKMGI LPEAMRNYLL RLGWSHGNDE IISSEQAVEW FNLENIGRSP ARLDFKKLKY LNNHYINNMN NKDILTLILR ENTLNDKKKN YLLHGLTELK KRASYLNELL DLAQFYIQDP PLDLNKEAEQ IVKSNLDTIK LLASFLLRIS HENWNKNSLP SQVKEFSKLH NVKISDVYHS LRAPITGTMD APGIINIMVT LGRDECIRRL RNYS

NT Sequence

atgttaagca taatcactag atttgctcca tctccaacag gttttttgca catcggtgga gctcgcactg ctttattcaa ttggctctac gcaaagcatc atggtggtaa gtttttacta aggattgagg acactgacaa gaaacgatca acacaaaagg caatcaatgc aataatagac ggcctgaaat ggcttgatgt gaattatgat gaagaaataa tataccaatc aaaaagaaca gagaggtatg ttgaagttgc aaacctttta gtcaaaagag gtaaggcgta ttactgctat tgccctgaaa ataaagttgc agaaaagaaa gcaaaagcaa gagaagaagg aaagatatac aagcataaat gcttcaatgt aacaccaaat gtaaagcctg ttgttcgatt taaagtccca gattcacaag atatcgttgt taacgataaa atatacggtc aaattaaagt aaatagcaaa caaattgatg acatagtaat tttacgttct gacaacactc caacatatat ttttgccgtg gtgattgatg atcatgatac tagaataact gatataatac gtggttctga ccatcttact aatactttta aacaattact gatatatcaa gcccttgatt ttgatattcc acgctttgca catgtaccgc tgattcacca ggagaatgga aataagctat ctaaaagaca tggtgcaaca agcatttgcg attataaaaa aatgggaata ttaccagagg caatgcgcaa ctacttacta agacttggct ggagtcatgg aaacgatgag attatcagta gtgagcaggc agtagagtgg ttcaatttag aaaacattgg tcgttcacct gcacgcctag attttaaaaa gctgaaatac ttaaataatc actatattaa taacatgaac aataaagaca ttctgactct aattcttaga gaaaatactc taaacgataa aaaaaagaac tatttactac acgggctaac ggaactaaaa aaaagagcaa gctacctaaa cgaattattg gatttagcac agttttatat tcaagatcca ccacttgatt taaacaaaga ggccgagcaa attgtcaaat ctaacctcga tacaatcaaa ttacttgcgt catttctctt gagaattagt catgaaaact ggaacaagaa ctctctacct tctcaagtca aggagttttc aaagttacat aatgttaaaa taagtgatgt gtaccactca ttacgcgccc ctataaccgg aacaatggat gcacctggga tcatcaatat aatggtaact cttggtagag acgagtgtat aagaagacta cggaactata gctga

Details for WoovA.01187.a.EW11.GE00526

HARVESTED ON:	12/17/2015
SEQUENCED ON:	5/4/2017
EXPECTED MW:	64kDa
OBSERVED MW:	64kDa
ANTIBIOTIC MARKER:	kanamycin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MGHHHHHHSG EVKPEVKPET HINLKVSDGS SEIFFKIKKT TPLRRLMEAF AKRQGKEMDS LRFLYDGIRI QADQTPEDLD MEDNDIIEAH REQIGGMLSI ITRFAPSPTG FLHIGGARTA LFNWLYAKHH GGKFLLRIED TDKKRSTQKA INAIIDGLKW LDVNYDEEII YQSKRTERYV EVANLLVKRG KAYYCYCPEN KVAEKKAKAR EEGKIYKHKC FNVTPNVKPV VRFKVPDSQD IVVNDKIYGQ IKVNSKQIDD IVILRSDNTP TYIFAVVIDD HDTRITDIIR GSDHLTNTFK QLLIYQALDF DIPRFAHVPL IHQENGNKLS KRHGATSICD YKKMGILPEA MRNYLLRLGW SHGNDEIISS EQAVEWFNLE NIGRSPARLD FKKLKYLNNH YINNMNNKDI LTLILRENTL NDKKKNYLLH GLTELKKRAS YLNELLDLAQ FYIQDPPLDL NKEAEQIVKS NLDTIKLLAS FLLRISHENW NKNSLPSQVK EFSKLHNVKI SDVYHSLRAP ITGTMDAPGI INIMVTLGRD ECIRRLRNYS

Full NT Sequence (Expression Vector + Insert)

tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg aattaattct tagaaaaact catcgagcat caaatgaaac tgcaatttat tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat gaaggagaaa actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg attccgactc gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta tcaagtgaga aatcaccatg agtgacgact gaatccggtg agaatggcaa aagtttatgc atttctttcc agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca tcaaccaaac cgttattcat tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca tcaacaatat tttcacctga atcaggatat tcttctaata cctggaatgc tgttttcccg gggatcgcag tggtgagtaa ccatgcatca tcaggagtac ggataaaatg cttgatggtc ggaagaggca taaattccgt cagccagttt agtctgacca tctcatctgt aacatcattg gcaacgctac ctttgccatg tttcagaaac aactctggcg catcgggctt cccatacaat cgatagattg tcgcacctga ttgcccgaca ttatcgcgag cccatttata cccatataaa tcagcatcca tgttggaatt taatcgcggc ctagagcaag acgtttcccg ttgaatatgg ctcataacac cccttgtatt actgtttatg taagcagaca gttttattgt tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgagatc ccggtgccta atgagtgagc taacttacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgccag ggtggttttt cttttcacca gtgagacggg caacagctga ttgcccttca ccgcctggcc ctgagagagt tgcagcaagc ggtccacgct ggtttgcccc agcaggcgaa aatcctgttt gatggtggtt aacggcggga tataacatga gctgtcttcg gtatcgtcgt atcccactac cgagatatcc gcaccaacgc gcagcccgga ctcggtaatg gcgcgcattg cgcccagcgc catctgatcg ttggcaacca gcatcgcagt gggaacgatg ccctcattca gcatttgcat ggtttgttga aaaccggaca tggcactcca gtcgccttcc cgttccgcta tcggctgaat ttgattgcga gtgagatatt tatgccagcc agccagacgc agacgcgccg agacagaact taatgggccc gctaacagcg cgatttgctg gtgacccaat gcgaccagat gctccacgcc cagtcgcgta ccgtcttcat gggagaaaat aatactgttg atgggtgtct ggtcagagac atcaagaaat aacgccggaa cattagtgca ggcagcttcc acagcaatgg catcctggtc atccagcgga tagttaatga tcagcccact gacgcgttgc gcgagaagat tgtgcaccgc cgctttacag gcttcgacgc cgcttcgttc taccatcgac accaccacgc tggcacccag ttgatcggcg cgagatttaa tcgccgcgac aatttgcgac ggcgcgtgca gggccagact ggaggtggca acgccaatca gcaacgactg tttgcccgcc agttgttgtg ccacgcggtt gggaatgtaa ttcagctccg ccatcgccgc ttccactttt tcccgcgttt tcgcagaaac gtggctggcc tggttcacca cgcgggaaac ggtctgataa gagacaccgg catactctgc gacatcgtat aacgttactg gtttcacatt caccaccctg aattgactct cttccgggcg ctatcatgcc ataccgcgaa aggttttgcg ccattcgatg gtgtccggga tctcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aattaatacg actcactata ggggaattgt gagcggataa caattcccct ctagaaataa ttttgtttaa ctttaagaag gagatatacc atgggccacc accaccacca ccatagcggc gaagtaaaac cggaagtgaa gccggaaacc cacatcaacc tgaaggttag cgacggtagc agcgaaatct tcttcaagat taagaaaacc accccgctgc gtcgcctgat ggaagcattc gctaaacgcc agggcaagga aatggattcc ctgcgctttc tgtacgacgg tatccgtatt caggcagacc agactccgga agatctggac atggaagata acgacattat cgaagcacac cgtgaacaaa tcggtggaat gttgtctatc attactcgtt tcgcaccttc cccaactggt ttcttgcaca tcggcggcgc gcgcacggca ctgtttaact ggctgtatgc gaaacatcac ggcggtaaat tcctgttgcg tattgaagat acggacaaaa agcgtagcac gcagaaagcc atcaatgcca tcattgacgg tctgaagtgg ctggacgtta actacgatga agaaatcatt tatcagagca agcgcaccga gcgttatgtc gaagtggcga atctgctggt gaagcgtggt aaagcgtact attgttattg tccggaaaac aaagttgcgg agaaaaaagc gaaagcccgc gaggagggca agatttacaa gcataagtgc tttaatgtta ccccgaacgt gaagccagtc gtccgtttta aggtcccgga cagccaagat attgtggtca acgacaaaat ctatggtcag attaaggtta atagcaaaca gatcgacgac attgtcattc tgcgtagcga caacaccccg acctacattt ttgcagtggt tatcgatgat cacgacaccc gtattacgga tatcatccgc ggtagcgatc atctgaccaa taccttcaag caattactga tctaccaggc cctggatttt gatattccgc gctttgcgca cgttccgctg atccaccaag agaacggcaa caagttgagc aaacgtcacg gtgctaccag catttgcgac tacaagaaaa tgggcatcct gccggaagca atgcgcaatt acctgttgcg tctgggttgg agccacggta atgatgaaat catttcttcc gagcaagccg ttgagtggtt caatctggag aatatcggtc gtagcccggc tcgtctggac ttcaaaaagc tgaagtacct gaataatcat tacattaaca atatgaataa caaagacatt ctgacgctga tcttgcgcga gaatacgctg aacgataaga aaaagaacta tttgctgcat ggcctgaccg agctgaaaaa gcgtgcgtct tacctgaatg aactgctgga cctggcacag ttctatatcc aagatccgcc gcttgacctg aacaaagagg cagagcaaat cgtgaaatcg aacctggaca ccatcaaact gctcgcgagc ttcctgctgc gtattagcca tgaaaactgg aacaagaaca gcctgccgag ccaggtcaaa gaattttcca agctgcacaa tgttaagatt agcgacgtgt accacagcct gcgtgcgccg attaccggca cgatggatgc tccgggtatt atcaatatca tggtgaccct gggtcgtgat gagtgcatcc gccgtctgcg caactacagc tgataacaag cttgcggccg cactcgagca ccaccaccac caccactgag atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggat