SSGCID: Target Page

WoovA.00145.a

aspartyl-tRNA synthetase

CENTER ID:	WoovA.00145.a
ORGANISM:	Wolbachia endosymbiont of Onchocerca volvulus
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	NA
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
WoovA.00145.a.EW11.GE00525	full length		1	600

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MNCYKTHTCN ELRKDDVEKE VTLSGWLYHK RNHGNLIFVD LRDFYGITQL VFNNDKDFFN EISNLKSESV ITVTGIVKAR TEDTVNSSIS TGEIEVIVSK LHIESKVEFH CDEEIAKEEK SILASISGKQ EYRENMRLKY RFLDLRREKV RNNIILRSQI IAELRRLMIE QGFLEIQTPI LTASSPEGAR DYLVPSRLNP GKFYALPQAP QIFKQLLMVS GFDKYFQIAP CFRDEDTRSD RSGEFYQLDL EMSFVTQEDI FQVIETTLYK VFTKFSCKSV DKNFPRITYK EAMLKYGSDK PDLRNPLSIS DVTEVFRDSK FNIFKSNIEK GMVVRAIPAP KTAKESRSFF DKKIEHVQKE FGAKGLGYIT FDKDGTIKGP IAKFFDKNKI NHIREITNVK PGDSIFFTSD KENEAAIIAG KVRTILGSEL GLIDKNVFKF CWIIDFPYFV YDYKNKKINF FHNPFSMPHG GLKDLEERNP LYILAYQYDL VCNGIELSSG AIRNNKLDIM YKAFAISGYS KEEVDTKFSA LVRAFRFGVP PHGGIAPGVD RMVMLLADEP NIREVICFPM NQQGEDVLMG APSKVKSRHL HELFLQVID

NT Sequence

atgaactgct ataaaactca tacctgcaat gaattaagaa aggatgatgt agaaaaagaa gttactcttt ctggatggct ataccacaaa cgtaaccatg gtaatctcat ctttgttgac ttaagagact tctacggaat tactcagcta gtattcaaca atgacaaaga ctttttcaat gaaatatcaa atttaaaatc agagagtgtg attactgtta caggaatagt caaagctaga actgaagata cagtaaacag ctctatttcc acaggagaaa ttgaggttat agttagtaaa ttgcacattg aatcgaaagt tgagtttcat tgtgacgaag aaatagcaaa agaggaaaaa agcatattag caagtatctc tggcaaacaa gaataccgag aaaatatgag acttaaatat cggtttcttg acttaaggcg ggaaaaagtt cgcaataaca tcattttgcg ctcacaaatc attgcagaac tacgtagact catgatagaa caaggttttt tagaaattca aactccaata ctcactgctt catctcctga aggagcacgc gattatttag ttccaagcag actaaatcct ggtaaattct atgcactacc acaagctcca caaattttta aacaactgct catggtgtca ggattcgata aatattttca aattgcacca tgtttccgtg atgaagacac aagatctgac cgttctggag aattttatca gctagatctt gaaatgtctt ttgtgactca agaagacata tttcaagtca tcgaaactac tttatacaag gtgttcacta aattttcttg caagtcagtc gacaagaact ttccacgcat tacatataaa gaagcaatgc tcaaatatgg ctctgacaaa ccagatctac gtaatccatt atcaattagt gatgttacag aagttttccg tgattcaaag ttcaacatct tcaaaagcaa cattgagaag ggtatggtag ttagagccat tcccgctccc aaaacagcaa aggagtcacg gagctttttt gataaaaaaa tagagcatgt gcaaaaagaa ttcggagcta aaggtcttgg atatataacg tttgataaag atggtactat aaaaggacca attgccaaat tctttgataa aaataagata aatcatataa gagaaataac aaatgtaaag cctggagata gtatattttt tacttccgat aaagaaaatg aagcagcaat aattgcagga aaagtgcgta ccattttagg atcagaatta ggtcttatag ataaaaatgt tttcaaattc tgttggatta ttgactttcc atattttgtg tatgattata aaaataaaaa aattaatttt tttcataacc cattttctat gccacatggt ggcttaaaag atctggagga aagaaatcca ctgtacattc ttgcttacca atatgatctc gtttgtaatg gaatagaact atcaagcgga gcaattcgca ataacaaact agatattatg tataaagctt ttgccatttc aggctatagt aaagaagaag tcgacacaaa atttagtgca ctcgtgcgtg cattcagatt tggagtacca cctcatggtg gaatagcacc gggagttgac agaatggtta tgctgctcgc tgatgagcca aacattcgtg aagtgatctg ttttcctatg aaccaacaag gtgaagatgt tctaatgggc gctccttcta aggtaaaaag tagacattta catgaattat tcttgcaggt tattgattaa

Details for WoovA.00145.a.EW11.GE00525

HARVESTED ON:	3/17/2016
SEQUENCED ON:	5/4/2017
EXPECTED MW:	80kDa
OBSERVED MW:	80kDa
ANTIBIOTIC MARKER:	kanamycin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MGHHHHHHSG EVKPEVKPET HINLKVSDGS SEIFFKIKKT TPLRRLMEAF AKRQGKEMDS LRFLYDGIRI QADQTPEDLD MEDNDIIEAH REQIGGMNCY KTHTCNELRK DDVEKEVTLS GWLYHKRNHG NLIFVDLRDF YGITQLVFNN DKDFFNEISN LKSESVITVT GIVKARTEDT VNSSISTGEI EVIVSKLHIE SKVEFHCDEE IAKEEKSILA SISGKQEYRE NMRLKYRFLD LRREKVRNNI ILRSQIIAEL RRLMIEQGFL EIQTPILTAS SPEGARDYLV PSRLNPGKFY ALPQAPQIFK QLLMVSGFDK YFQIAPCFRD EDTRSDRSGE FYQLDLEMSF VTQEDIFQVI ETTLYKVFTK FSCKSVDKNF PRITYKEAML KYGSDKPDLR NPLSISDVTE VFRDSKFNIF KSNIEKGMVV RAIPAPKTAK ESRSFFDKKI EHVQKEFGAK GLGYITFDKD GTIKGPIAKF FDKNKINHIR EITNVKPGDS IFFTSDKENE AAIIAGKVRT ILGSELGLID KNVFKFCWII DFPYFVYDYK NKKINFFHNP FSMPHGGLKD LEERNPLYIL AYQYDLVCNG IELSSGAIRN NKLDIMYKAF AISGYSKEEV DTKFSALVRA FRFGVPPHGG IAPGVDRMVM LLADEPNIRE VICFPMNQQG EDVLMGAPSK VKSRHLHELF LQVID

Full NT Sequence (Expression Vector + Insert)

tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg aattaattct tagaaaaact catcgagcat caaatgaaac tgcaatttat tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat gaaggagaaa actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg attccgactc gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta tcaagtgaga aatcaccatg agtgacgact gaatccggtg agaatggcaa aagtttatgc atttctttcc agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca tcaaccaaac cgttattcat tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca tcaacaatat tttcacctga atcaggatat tcttctaata cctggaatgc tgttttcccg gggatcgcag tggtgagtaa ccatgcatca tcaggagtac ggataaaatg cttgatggtc ggaagaggca taaattccgt cagccagttt agtctgacca tctcatctgt aacatcattg gcaacgctac ctttgccatg tttcagaaac aactctggcg catcgggctt cccatacaat cgatagattg tcgcacctga ttgcccgaca ttatcgcgag cccatttata cccatataaa tcagcatcca tgttggaatt taatcgcggc ctagagcaag acgtttcccg ttgaatatgg ctcataacac cccttgtatt actgtttatg taagcagaca gttttattgt tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgagatc ccggtgccta atgagtgagc taacttacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgccag ggtggttttt cttttcacca gtgagacggg caacagctga ttgcccttca ccgcctggcc ctgagagagt tgcagcaagc ggtccacgct ggtttgcccc agcaggcgaa aatcctgttt gatggtggtt aacggcggga tataacatga gctgtcttcg gtatcgtcgt atcccactac cgagatatcc gcaccaacgc gcagcccgga ctcggtaatg gcgcgcattg cgcccagcgc catctgatcg ttggcaacca gcatcgcagt gggaacgatg ccctcattca gcatttgcat ggtttgttga aaaccggaca tggcactcca gtcgccttcc cgttccgcta tcggctgaat ttgattgcga gtgagatatt tatgccagcc agccagacgc agacgcgccg agacagaact taatgggccc gctaacagcg cgatttgctg gtgacccaat gcgaccagat gctccacgcc cagtcgcgta ccgtcttcat gggagaaaat aatactgttg atgggtgtct ggtcagagac atcaagaaat aacgccggaa cattagtgca ggcagcttcc acagcaatgg catcctggtc atccagcgga tagttaatga tcagcccact gacgcgttgc gcgagaagat tgtgcaccgc cgctttacag gcttcgacgc cgcttcgttc taccatcgac accaccacgc tggcacccag ttgatcggcg cgagatttaa tcgccgcgac aatttgcgac ggcgcgtgca gggccagact ggaggtggca acgccaatca gcaacgactg tttgcccgcc agttgttgtg ccacgcggtt gggaatgtaa ttcagctccg ccatcgccgc ttccactttt tcccgcgttt tcgcagaaac gtggctggcc tggttcacca cgcgggaaac ggtctgataa gagacaccgg catactctgc gacatcgtat aacgttactg gtttcacatt caccaccctg aattgactct cttccgggcg ctatcatgcc ataccgcgaa aggttttgcg ccattcgatg gtgtccggga tctcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aattaatacg actcactata ggggaattgt gagcggataa caattcccct ctagaaataa ttttgtttaa ctttaagaag gagatatacc atgggccacc accaccacca ccatagcggc gaagtaaaac cggaagtgaa gccggaaacc cacatcaacc tgaaggttag cgacggtagc agcgaaatct tcttcaagat taagaaaacc accccgctgc gtcgcctgat ggaagcattc gctaaacgcc agggcaagga aatggattcc ctgcgctttc tgtacgacgg tatccgtatt caggcagacc agactccgga agatctggac atggaagata acgacattat cgaagcacac cgtgaacaaa tcggtggaat gaactgctac aaaacgcaca cctgtaacga actgcgcaaa gacgatgttg agaaagaagt caccctgagc ggctggctgt accacaagcg caatcacggc aatctgattt ttgtggatct gcgcgacttt tatggtatca cccaactggt gttcaataat gataaagact ttttcaatga gattagcaac ctgaaaagcg agagcgttat taccgtcacg ggtattgtga aggctcgtac cgaggacacc gtgaacagca gcatttccac tggtgagatt gaagtgatcg ttagcaagct ccacatcgag agcaaagttg agttccattg cgacgaagaa attgcgaagg aagagaaaag cattctggca tctatctctg gcaaacaaga gtaccgtgaa aacatgcgtc tgaagtatcg ttttctggat ttgcgtcgcg aaaaagttcg caacaatatc atcctgcgta gccagatcat cgctgagtta cgtcgtctga tgatcgagca aggtttcctg gaaattcaga cgccgatcct gaccgcgtct agccctgagg gtgcccgtga ttacttggtc ccgtcgcgcc tgaacccggg caagttctac gcgctgccgc aagcgccgca gattttcaag caactgctga tggtgagcgg ctttgacaaa tactttcaga tcgcaccgtg tttccgtgac gaagatacgc gctccgaccg ttcgggtgag ttttatcagc tggatttgga gatgtccttc gttacgcaag aggacatttt ccaggtcatt gagactaccc tgtataaagt ctttaccaaa tttagctgca agagcgttga taagaatttc ccacgcatca cctacaagga agcaatgctg aaatatggta gcgataaacc ggatctgcgc aatccgctga gcatcagcga cgtgaccgaa gtgtttcgtg attcaaagtt taacatcttc aaatccaata ttgagaaagg tatggttgtc cgtgccatcc cggctccgaa aaccgcgaag gaaagccgta gctttttcga taagaaaatc gaacacgttc agaaagagtt cggtgcgaag ggcttgggtt atatcacgtt tgataaggac ggcacgatta agggtccgat cgcgaagttc tttgacaaga acaagatcaa ccacattcgc gagattacca atgtgaaacc gggtgactct atcttcttta ccagcgataa agagaatgaa gcggcgatta ttgcgggtaa ggtccgcacg atcctgggca gcgagctggg tctgattgac aaaaacgttt tcaagttttg ctggatcatc gacttcccgt acttcgttta tgactacaag aacaaaaaga ttaacttctt ccataatcca ttctccatgc cgcacggtgg cctgaaagat ttggaggaac gtaacccgct gtacatcctg gcatatcagt acgacctggt gtgcaacggc attgaactga gctctggcgc cattcgcaat aacaaactgg acatcatgta taaggcattt gccattagcg gttacagcaa agaggaagtg gataccaagt tcagcgcctt ggtccgtgcg tttcgtttcg gtgttcctcc gcatggcggt attgctccgg gcgtggaccg tatggtcatg ctgcttgcag atgagccgaa tattcgtgaa gtcatttgtt ttccgatgaa tcaacagggt gaagatgttc tgatgggtgc gccaagcaaa gtgaagtcgc gtcatctgca tgaactgttc ctgcaagtga tcgactgata acaagcttgc ggccgcactc gagcaccacc accaccacca ctgagatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat