TrvaA.18681.a

Anticodon binding domain containing protein; Prolyl-tRNA synthetase (ProRS)

CENTER ID: TrvaA.18681.a
ORGANISM: Trichomonas vaginalis ATCC PRA-98 / G3
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: unclassified
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
TrvaA.18681.a.B1.GE39015 full length 1 513
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
TrvaA.18681.a.B1.PW37738 full length 1 513

External Resources

RESOURCE REFERENCE ID
EuPathDB: TrichDB:TVAG_053680
RefSeq: XP_001308783.1
UniProt: A2FGU9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTEKEEVPVV EQKGKPQKQK GQKKEKPQKQ QGKQEKKQAQ ASQRADLTFH VPKSNFSDWY EELMVTAGIV DKRWDIKGMP IFCSYGYTMH NEIMHLFEVA YSKIGVPMTQ FPAFIPRSFL EAEADHVKGF QSECFWHEDE MCLRPTSETA MYSMFAKWVK TIEDLPVMVQ QSCNVYRYET KGTKPLIRVR EIPWNEAHTA HATPQEALDI LGKFWDIVHD ILTTKLSIDG LRLRRPDWDR FPGAEFTDVL DVVMPCGRVL QSVGAHYLGQ HFSKVFKIQV DGEFSYMTCI GVSTRCLAAV LSAHGDDKGL IIPTIIAPIQ VVIVPILRKD NDALLAKVAE MKKELRAAGI RVHIDDDTTR SPGEKFYYWE MKGVPLRIEV GPRDLEKNEC VLVVRGIEGK YSVHLSAFAQ ECQDWLVKYD DALRERALAH LHSRIVDCNN LDDLPGILEK GGFARIPYWT MGKEGKEGAE RVHDICGGEI RGYRPFEEQP AEGVKCLLTG KPAKFWAYVA RSY
NT Sequence
atgactgaga aagaagaagt cccagttgtt gagcagaagg gaaagcccca aaagcaaaag ggccagaaga aggagaagcc acagaagcaa caaggaaagc aggagaagaa gcaagcccaa gcttcacagc gtgctgatct taccttccac gttccaaaat caaacttttc agattggtac gaagagctca tggtcacagc cggtatcgtt gataagcgct gggatatcaa gggcatgcca attttctgct cttatggtta tacaatgcac aacgaaatta tgcatctctt cgaggttgcc tactccaaga tcggagttcc aatgacacaa ttcccagctt tcattccacg ttcattcctt gaagccgaag ctgatcacgt taagggcttc caatccgagt gtttctggca tgaggacgag atgtgccttc gtccaacatc agagacagcc atgtactcca tgttcgcaaa gtgggttaag acaattgaag accttccagt catggttcag caatcctgca acgtttaccg ttacgaaaca aagggaacaa agccacttat ccgtgttcgt gaaattccat ggaatgaggc ccatacagcc cacgccacac cacaagaggc tcttgacatc ctcggcaagt tctgggacat cgtccacgat attcttacaa caaagctttc catcgacggc cttcgtcttc gccgtccaga ttgggaccgt ttcccaggcg ctgagttcac agatgttctt gatgttgtca tgccatgtgg ccgtgttctt cagagtgtcg gtgctcacta ccttggccag catttcagca aagttttcaa gatccaagtt gatggcgaat tctcttacat gacctgcatc ggtgtttcca caagatgcct cgctgcagtc ctctccgctc acggtgatga caagggtctc attatcccaa caatcatcgc cccaatccag gttgtcattg tcccaattct ccgcaaggac aacgacgctt tactcgccaa ggtcgctgag atgaagaagg aactcagagc cgctggaatc cgtgtccaca tcgatgatga tacaacacgc agcccaggcg agaagttcta ctactgggag atgaagggtg ttccactccg tatcgaagtt ggcccacgcg atctcgaaaa gaacgaatgc gttctcgtcg tcagaggaat cgaaggaaag tactcagtcc acctctccgc attcgctcag gaatgccagg actggctcgt caagtacgat gacgctctcc gtgaacgcgc tctcgctcac cttcattccc gcatcgtcga ttgcaacaac ctcgacgatc tcccaggaat cctcgaaaag ggtggcttcg caagaatccc atactggaca atgggcaagg aaggtaagga aggagctgag agagtccacg acatctgcgg tggtgaaatc cgtggctaca gaccattcga agaacagcca gctgaaggtg ttaagtgcct ccttacagga aagccagcta agttctgggc ttacgtcgct cgttcatact aa
Details for TrvaA.18681.a.B1.GE39015
HARVESTED ON: 3/4/2015
SEQUENCED ON: 3/4/2015
EXPECTED MW: 60kDa
OBSERVED MW: 55kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 90
PERCENT COVERAGE: 60
Validated AA Sequence
MAHHHHHHMT EKEEVPVVEQ KGKPQKQKGQ KKEKPQKQQG KQEKKQAQAS QRADLTFHVP KSNFSDWYEE LMVTAGIVDK RWDIKGMPIF CSYGYTMHNE IMHLFEVAYS KIGVPMTQFP AFIPRSFLEA EADHVKGFQS ECFWHEDEMC LRPTSETAMY SMFAKWVKTI EDLPVMVQQS CNVYRYETKG TKPLIRVREI PWNEAHTAHA TPQEALDILG KFWDIVHDIL TTKLSIDGLR LRRPDWDRFP GAEFTDVLDV VMPCGRVLQS VGAHYLXXHX XXXFKXXVXX EFSYMXXXXX XXXXXXXXXX XXXXX
Validated NT Sequence
atggctcacc accaccacca ccatatgact gagaaagaag aagtcccagt tgttgagcag aagggaaagc cccaaaagca aaagggccag aagaaggaga agccacagaa gcaacaagga aagcaggaga agaagcaagc ccaagcttca cagcgtgctg atcttacctt ccacgttcca aaatcaaact tttcagattg gtacgaagag ctcatggtca cagccggtat cgttgataag cgctgggata tcaagggcat gccaattttc tgctcttatg gttatacaat gcacaacgaa attatgcatc tcttcgaggt tgcctactcc aagatcggag ttccaatgac acaattccca gctttcattc cacgttcatt ccttgaagcc gaagctgatc acgttaaggg cttccaatcc gagtgtttct ggcatgagga cgagatgtgc cttcgtccaa catcagagac agccatgtac tccatgttcg caaagtgggt taagacaatt gaagaccttc cagtcatggt tcagcaatcc tgcaacgttt accgttacga aacaaaggga acaaagccac ttatccgtgt tcgtgaaatt ccatggaatg aggcccatac agcccacgcc acaccacaag aggctcttga catcctcggc aagttctggg acatcgtcca cgatattctt acaacaaagc tttccatcga cggccttcgt cttcgccgtc cagattggga ccgtttccca ggcgctgagt tcacagatgt tcttgatgtt gtcatgccat gtggccgtgt tcttcagagt gtcggtgctc actacctnnn nnagcattnn nnnnnnnttt tcaagntcnn agttgannnn gaattctctt acatgannng catnnnnnnn nnnnnnnnnn nnnnnnntgn nnnnnnnnnn nnnnnnnnnn nnnnnna
Expected Protein Sequence
MAHHHHHHMT EKEEVPVVEQ KGKPQKQKGQ KKEKPQKQQG KQEKKQAQAS QRADLTFHVP KSNFSDWYEE LMVTAGIVDK RWDIKGMPIF CSYGYTMHNE IMHLFEVAYS KIGVPMTQFP AFIPRSFLEA EADHVKGFQS ECFWHEDEMC LRPTSETAMY SMFAKWVKTI EDLPVMVQQS CNVYRYETKG TKPLIRVREI PWNEAHTAHA TPQEALDILG KFWDIVHDIL TTKLSIDGLR LRRPDWDRFP GAEFTDVLDV VMPCGRVLQS VGAHYLGQHF SKVFKIQVDG EFSYMTCIGV STRCLAAVLS AHGDDKGLII PTIIAPIQVV IVPILRKDND ALLAKVAEMK KELRAAGIRV HIDDDTTRSP GEKFYYWEMK GVPLRIEVGP RDLEKNECVL VVRGIEGKYS VHLSAFAQEC QDWLVKYDDA LRERALAHLH SRIVDCNNLD DLPGILEKGG FARIPYWTMG KEGKEGAERV HDICGGEIRG YRPFEEQPAE GVKCLLTGKP AKFWAYVARS Y
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgactg agaaagaaga agtcccagtt gttgagcaga agggaaagcc ccaaaagcaa aagggccaga agaaggagaa gccacagaag caacaaggaa agcaggagaa gaagcaagcc caagcttcac agcgtgctga tcttaccttc cacgttccaa aatcaaactt ttcagattgg tacgaagagc tcatggtcac agccggtatc gttgataagc gctgggatat caagggcatg ccaattttct gctcttatgg ttatacaatg cacaacgaaa ttatgcatct cttcgaggtt gcctactcca agatcggagt tccaatgaca caattcccag ctttcattcc acgttcattc cttgaagccg aagctgatca cgttaagggc ttccaatccg agtgtttctg gcatgaggac gagatgtgcc ttcgtccaac atcagagaca gccatgtact ccatgttcgc aaagtgggtt aagacaattg aagaccttcc agtcatggtt cagcaatcct gcaacgttta ccgttacgaa acaaagggaa caaagccact tatccgtgtt cgtgaaattc catggaatga ggcccataca gcccacgcca caccacaaga ggctcttgac atcctcggca agttctggga catcgtccac gatattctta caacaaagct ttccatcgac ggccttcgtc ttcgccgtcc agattgggac cgtttcccag gcgctgagtt cacagatgtt cttgatgttg tcatgccatg tggccgtgtt cttcagagtg tcggtgctca ctaccttggc cagcatttca gcaaagtttt caagatccaa gttgatggcg aattctctta catgacctgc atcggtgttt ccacaagatg cctcgctgca gtcctctccg ctcacggtga tgacaagggt ctcattatcc caacaatcat cgccccaatc caggttgtca ttgtcccaat tctccgcaag gacaacgacg ctttactcgc caaggtcgct gagatgaaga aggaactcag agccgctgga atccgtgtcc acatcgatga tgatacaaca cgcagcccag gcgagaagtt ctactactgg gagatgaagg gtgttccact ccgtatcgaa gttggcccac gcgatctcga aaagaacgaa tgcgttctcg tcgtcagagg aatcgaagga aagtactcag tccacctctc cgcattcgct caggaatgcc aggactggct cgtcaagtac gatgacgctc tccgtgaacg cgctctcgct caccttcatt cccgcatcgt cgattgcaac aacctcgacg atctcccagg aatcctcgaa aagggtggct tcgcaagaat cccatactgg acaatgggca aggaaggtaa ggaaggagct gagagagtcc acgacatctg cggtggtgaa atccgtggct acagaccatt cgaagaacag ccagctgaag gtgttaagtg cctccttaca ggaaagccag ctaagttctg ggcttacgtc gctcgttcat actgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat
Details for TrvaA.18681.a.B1.PW37738
PURIFICATION DATe: 7/7/2015
CONCENTRATION: 16.5mg/ml
OBSERVED MW: 64kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 3
VIAL VOLUME: 200µl
PERCENT IDENTITY: 90
PERCENT COVERAGE: 60
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMT EKEEVPVVEQ KGKPQKQKGQ KKEKPQKQQG KQEKKQAQAS QRADLTFHVP KSNFSDWYEE LMVTAGIVDK RWDIKGMPIF CSYGYTMHNE IMHLFEVAYS KIGVPMTQFP AFIPRSFLEA EADHVKGFQS ECFWHEDEMC LRPTSETAMY SMFAKWVKTI EDLPVMVQQS CNVYRYETKG TKPLIRVREI PWNEAHTAHA TPQEALDILG KFWDIVHDIL TTKLSIDGLR LRRPDWDRFP GAEFTDVLDV VMPCGRVLQS VGAHYLXXHX XXXFKXXVXX EFSYMXXXXX XXXXXXXXXX XXXXX
Validated NT Sequence
atggctcacc accaccacca ccatatgact gagaaagaag aagtcccagt tgttgagcag aagggaaagc cccaaaagca aaagggccag aagaaggaga agccacagaa gcaacaagga aagcaggaga agaagcaagc ccaagcttca cagcgtgctg atcttacctt ccacgttcca aaatcaaact tttcagattg gtacgaagag ctcatggtca cagccggtat cgttgataag cgctgggata tcaagggcat gccaattttc tgctcttatg gttatacaat gcacaacgaa attatgcatc tcttcgaggt tgcctactcc aagatcggag ttccaatgac acaattccca gctttcattc cacgttcatt ccttgaagcc gaagctgatc acgttaaggg cttccaatcc gagtgtttct ggcatgagga cgagatgtgc cttcgtccaa catcagagac agccatgtac tccatgttcg caaagtgggt taagacaatt gaagaccttc cagtcatggt tcagcaatcc tgcaacgttt accgttacga aacaaaggga acaaagccac ttatccgtgt tcgtgaaatt ccatggaatg aggcccatac agcccacgcc acaccacaag aggctcttga catcctcggc aagttctggg acatcgtcca cgatattctt acaacaaagc tttccatcga cggccttcgt cttcgccgtc cagattggga ccgtttccca ggcgctgagt tcacagatgt tcttgatgtt gtcatgccat gtggccgtgt tcttcagagt gtcggtgctc actacctnnn nnagcattnn nnnnnnnttt tcaagntcnn agttgannnn gaattctctt acatgannng catnnnnnnn nnnnnnnnnn nnnnnnntgn nnnnnnnnnn nnnnnnnnnn nnnnnna
Expressed Protein Sequence
MAHHHHHHMT EKEEVPVVEQ KGKPQKQKGQ KKEKPQKQQG KQEKKQAQAS QRADLTFHVP KSNFSDWYEE LMVTAGIVDK RWDIKGMPIF CSYGYTMHNE IMHLFEVAYS KIGVPMTQFP AFIPRSFLEA EADHVKGFQS ECFWHEDEMC LRPTSETAMY SMFAKWVKTI EDLPVMVQQS CNVYRYETKG TKPLIRVREI PWNEAHTAHA TPQEALDILG KFWDIVHDIL TTKLSIDGLR LRRPDWDRFP GAEFTDVLDV VMPCGRVLQS VGAHYLGQHF SKVFKIQVDG EFSYMTCIGV STRCLAAVLS AHGDDKGLII PTIIAPIQVV IVPILRKDND ALLAKVAEMK KELRAAGIRV HIDDDTTRSP GEKFYYWEMK GVPLRIEVGP RDLEKNECVL VVRGIEGKYS VHLSAFAQEC QDWLVKYDDA LRERALAHLH SRIVDCNNLD DLPGILEKGG FARIPYWTMG KEGKEGAERV HDICGGEIRG YRPFEEQPAE GVKCLLTGKP AKFWAYVARS Y
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGACTG AGAAAGAAGA AGTCCCAGTT GTTGAGCAGA AGGGAAAGCC CCAAAAGCAA AAGGGCCAGA AGAAGGAGAA GCCACAGAAG CAACAAGGAA AGCAGGAGAA GAAGCAAGCC CAAGCTTCAC AGCGTGCTGA TCTTACCTTC CACGTTCCAA AATCAAACTT TTCAGATTGG TACGAAGAGC TCATGGTCAC AGCCGGTATC GTTGATAAGC GCTGGGATAT CAAGGGCATG CCAATTTTCT GCTCTTATGG TTATACAATG CACAACGAAA TTATGCATCT CTTCGAGGTT GCCTACTCCA AGATCGGAGT TCCAATGACA CAATTCCCAG CTTTCATTCC ACGTTCATTC CTTGAAGCCG AAGCTGATCA CGTTAAGGGC TTCCAATCCG AGTGTTTCTG GCATGAGGAC GAGATGTGCC TTCGTCCAAC ATCAGAGACA GCCATGTACT CCATGTTCGC AAAGTGGGTT AAGACAATTG AAGACCTTCC AGTCATGGTT CAGCAATCCT GCAACGTTTA CCGTTACGAA ACAAAGGGAA CAAAGCCACT TATCCGTGTT CGTGAAATTC CATGGAATGA GGCCCATACA GCCCACGCCA CACCACAAGA GGCTCTTGAC ATCCTCGGCA AGTTCTGGGA CATCGTCCAC GATATTCTTA CAACAAAGCT TTCCATCGAC GGCCTTCGTC TTCGCCGTCC AGATTGGGAC CGTTTCCCAG GCGCTGAGTT CACAGATGTT CTTGATGTTG TCATGCCATG TGGCCGTGTT CTTCAGAGTG TCGGTGCTCA CTACCTTGGC CAGCATTTCA GCAAAGTTTT CAAGATCCAA GTTGATGGCG AATTCTCTTA CATGACCTGC ATCGGTGTTT CCACAAGATG CCTCGCTGCA GTCCTCTCCG CTCACGGTGA TGACAAGGGT CTCATTATCC CAACAATCAT CGCCCCAATC CAGGTTGTCA TTGTCCCAAT TCTCCGCAAG GACAACGACG CTTTACTCGC CAAGGTCGCT GAGATGAAGA AGGAACTCAG AGCCGCTGGA ATCCGTGTCC ACATCGATGA TGATACAACA CGCAGCCCAG GCGAGAAGTT CTACTACTGG GAGATGAAGG GTGTTCCACT CCGTATCGAA GTTGGCCCAC GCGATCTCGA AAAGAACGAA TGCGTTCTCG TCGTCAGAGG AATCGAAGGA AAGTACTCAG TCCACCTCTC CGCATTCGCT CAGGAATGCC AGGACTGGCT CGTCAAGTAC GATGACGCTC TCCGTGAACG CGCTCTCGCT CACCTTCATT CCCGCATCGT CGATTGCAAC AACCTCGACG ATCTCCCAGG AATCCTCGAA AAGGGTGGCT TCGCAAGAAT CCCATACTGG ACAATGGGCA AGGAAGGTAA GGAAGGAGCT GAGAGAGTCC ACGACATCTG CGGTGGTGAA ATCCGTGGCT ACAGACCATT CGAAGAACAG CCAGCTGAAG GTGTTAAGTG CCTCCTTACA GGAAAGCCAG CTAAGTTCTG GGCTTACGTC GCTCGTTCAT ACTGAGTAAG ATAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAATT CTTGAAGACG AAAGGGCCTC GTGATACGCC TATTTTTATA GGTTAATGTC ATGATAATAA TGGTTTCTTA GACGTCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAAGTAA AAGATGCTGA AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT CTCAACAGCG GTAAGATCCT TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC ACTTTTAAAG TTCTGCTATG TGGCGCGGTA TTATCCCGTG TTGACGCCGG GCAAGAGCAA CTCGGTCGCC GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA AAGCATCTTA CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT GATAACACTG CGGCCAACTT ACTTCTGACA ACGATCGGAG GACCGAAGGA GCTAACCGCT TTTTTGCACA ACATGGGGGA TCATGTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC CAAACGACGA GCGTGACACC ACGATGCCTG CAGCAATGGC AACAACGTTG CGCAAACTAT TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG ATGGAGGCGG ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT ATTGCTGATA AATCTGGAGC CGGTGAGCGT GGGTCTCGCG GTATCATTGC AGCACTGGGG CCAGATGGTA AGCCCTCCCG TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACGAA ATAGACAGAT CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG TCAGACCAAG TTTACTCATA TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA AGGATCTAGG TGAAGATCCT TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTCCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCT ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA ACCGTATTAC CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACG ACCGAGCGCA GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC GCCTGATGCG GTATTTTCTC CTTACGCATC TGTGCGGTAT TTCACACCGC ATATATGGTG CACTCTCAGT ACAATCTGCT CTGATGCCGC ATAGTTAAGC CAGTATACAC TCCGCTATCG CTACGTGACT GGGTCATGGC TGCGCCCCGA CACCCGCCAA CACCCGCTGA CGCGCCCTGA CGGGCTTGTC TGCTCCCGGC ATCCGCTTAC AGACAAGCTG TGACCGTCTC CGGGAGCTGC ATGTGTCAGA GGTTTTCACC GTCATCACCG AAACGCGCGA GGCAGCTGCG GTAAAGCTCA TCAGCGTGGT CGTGAAGCGA TTCACAGATG TCTGCCTGTT CATCCGCGTC CAGCTCGTTG AGTTTCTCCA GAAGCGTTAA TGTCTGGCTT CTGATAAAGC GGGCCATGTT AAGGGCGGTT TTTTCCTGTT TGGTCACTGA TGCCTCCGTG TAAGGGGGAT TTCTGTTCAT GGGGGTAATG ATACCGATGA AACGAGAGAG GATGCTCACG ATACGGGTTA CTGATGATGA ACATGCCCGG TTACTGGAAC GTTGTGAGGG TAAACAACTG GCGGTATGGA TGCGGCGGGA CCAGAGAAAA ATCACTCAGG GTCAATGCCA GCGCTTCGTT AATACAGATG TAGGTGTTCC ACAGGGTAGC CAGCAGCATC CTGCGATGCA GATCCGGAAC ATAATGGTGC AGGGCGCTGA CTTCCGCGTT TCCAGACTTT ACGAAACACG GAAACCGAAG ACCATTCATG TTGTTGCTCA GGTCGCAGAC GTTTTGCAGC AGCAGTCGCT TCACGTTCGC TCGCGTATCG GTGATTCATT CTGCTAACCA GTAAGGCAAC CCCGCCAGCC TAGCCGGGTC CTCAACGACA GGAGCACGAT CATGCGCACC CGTGGCCAGG ACCCAACGCT GCCCGAGATG CGCCGCGTGC GGCTGCTGGA GATGGCGGAC GCGATGGATA TGTTCTGCCA AGGGTTGGTT TGCGCATTCA CAGTTCTCCG CAAGAATTGA TTGGCTCCAA TTCTTGGAGT GGTGAATCCG TTAGCGAGGT GCCGCCGGCT TCCATTCAGG TCGAGGTGGC CCGGCTCCAT GCACCGCGAC GCAACGCGGG GAGGCAGACA AGGTATAGGG CGGCGCCTAC AATCCATGCC AACCCGTTCC ATGTGCTCGC CGAGGCGGCA TAAATCGCCG TGACGATCAG CGGTCCAGTG ATCGAAGTTA GGCTGGTAAG AGCCGCGAGC GATCCTTGAA GCTGTCCCTG ATGGTCGTCA TCTACCTGCC TGGACAGCAT GGCCTGCAAC GCGGGCATCC CGATGCCGCC GGAAGCGAGA AGAATCATAA TGGGGAAGGC CATCCAGCCT CGCGTCGCGA ACGCCAGCAA GACGTAGCCC AGCGCGTCGG CCGCCATGCC GGCGATAATG GCCTGCTTCT CGCCGAAACG TTTGGTGGCG GGACCAGTGA CGAAGGCTTG AGCGAGGGCG TGCAAGATTC CGAATACCGC AAGCGACAGG CCGATCATCG TCGCGCTCCA GCGAAAGCGG TCCTCGCCGA AAATGACCCA GAGCGCTGCC GGCACCTGTC CTACGAGTTG CATGATAAAG AAGACAGTCA TAAGTGCGGC GACGATAGTC ATGCCCCGCG CCCACCGGAA GGAGCTGACT GGGTTGAAGG CTCTCAAGGG CATCGGTCGA CGCTCTCCCT TATGCGACTC CTGCATTAGG AAGCAGCCCA GTAGTAGGTT GAGGCCGTTG AGCACCGCCG CCGCAAGGAA TGGTGCATGC AAGGAGATGG CGCCCAACAG TCCCCCGGCC ACGGGGCCTG CCACCATACC CACGCCGAAA CAAGCGCTCA TGAGCCCGAA GTGGCGAGCC CGATCTTCCC CATCGGTGAT GTCGGCGATA TAGGCGCCAG CAACCGCACC TGTGGCGCCG GTGATGCCGG CCACGATGCG TCCGGCGTAG AGGATCGAGA TCTCGATCCC GCGAAAT