TrvaA.00429.d

Pyrophosphate--fructose 6-phosphate 1-phosphotransferase 1 (EC 2.7.1.90) (6-phosphofructokinase, pyrophosphate dependent 1) (PPi-dependent phosphofructokinase 1) (PPi-PFK 1) (Pyrophosphate-dependent 6-phosphofructose-1-kinase 1)

CENTER ID: TrvaA.00429.d
ORGANISM: Trichomonas vaginalis ATCC PRA-98 / G3
ASSOCIATED DISEASE:
CURRENT STATUS: in PDB
COMMUNITY REQUEST: False
NIH RISK GROUP: unclassified
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

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Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
TrvaA.00429.d.B1.PW39248 Full length(TrvaA.00429.d) 1 426

Structures

9DQM
DEPOSITED: 9/24/2024
DETERMINATION: XRay
CLONE: TrvaA.00429.d.B1.GE45123
PROTEIN: TrvaA.00429.d.B1.PW39248

External Resources

RESOURCE REFERENCE ID
EuPathDB: TrichDB:TVAG_364620
EuPathDB: TrichDB:TVAGG3_1029980
UniProt: O61068

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSTEAPVLGI LCGGGPAPGL NGVIAGATLY ALRLGWKVIG FMEGFKYLCT GDVDVVKAHT IDLTYDIVSR IHFQGGTIIQ TSRANPRKSP ELQENVRKCL RALKVRYFLT IGGDDTASSA VSVASGMNGN EISVISCPKT IDNDLPLPAD QSTFGFHTAR SLGMEIIRNL MVDSKSAPRW FLVEAMGRSA GHLALGMAEA SGAHLCLIPE EFKQDEIEFE DVVELVEATI LKRLAYGKNY GVCVLAEGLV SKMSKKALYK LFGNREPPTD PHGHILLDDA ELARSLSEEL LKRLGNLGIR ITPKKIGYEL RCADPVAFDA VYTRELGYGA IDAFLNGHSA ALIVRENGQV KPVQFKDLLD PATGRVRTRL VDVTSQSFKV ARVYMWRMSK KDYENKDLVA RVAAAGKMTP EAFTEKFAHL TDVVVE
NT Sequence
ATGAGCACTG AAGCTCCTGT TCTCGGTATC CTCTGCGGTG GTGGTCCAGC CCCAGGCCTC AATGGTGTCA TCGCTGGCGC TACACTTTAC GCCCTTAGAC TTGGCTGGAA GGTCATCGGC TTCATGGAAG GTTTCAAGTA CCTTTGCACA GGCGATGTTG ATGTCGTCAA GGCCCACACA ATCGACCTCA CATACGATAT CGTTTCTCGT ATCCACTTCC AGGGTGGCAC AATCATCCAA ACATCCCGTG CTAACCCACG TAAGTCCCCA GAACTCCAAG AGAACGTCCG CAAGTGCCTC CGCGCTCTCA AGGTTCGTTA CTTCCTTACA ATCGGTGGTG ATGATACAGC TTCATCCGCT GTTTCCGTCG CTTCCGGCAT GAACGGCAAC GAGATCTCTG TCATCTCCTG CCCAAAGACA ATCGATAACG ATCTTCCACT CCCAGCTGAC CAGTCCACAT TCGGCTTCCA CACAGCTCGC TCCCTTGGTA TGGAAATCAT CCGCAACCTC ATGGTTGACT CCAAGTCTGC CCCACGTTGG TTCCTTGTCG AAGCTATGGG CCGCTCTGCT GGTCACCTTG CTCTCGGCAT GGCTGAAGCT TCTGGTGCCC ACCTTTGCCT CATCCCAGAA GAATTCAAGC AGGACGAAAT CGAATTCGAA GATGTTGTTG AACTCGTCGA AGCTACAATC CTTAAGCGCC TTGCTTACGG AAAGAACTAC GGTGTCTGCG TTCTTGCAGA AGGCCTCGTT TCCAAGATGT CCAAGAAGGC TCTCTACAAG CTCTTCGGCA ACCGCGAACC ACCAACAGAT CCACACGGCC ACATCCTCCT CGATGATGCT GAACTCGCTC GTTCTCTCTC CGAGGAACTC CTCAAGCGCC TTGGCAACCT CGGCATCAGA ATCACACCAA AGAAGATCGG TTACGAACTT CGTTGCGCTG ATCCAGTCGC CTTCGATGCT GTCTACACAC GTGAGCTCGG CTACGGTGCT ATCGATGCCT TCCTCAACGG CCACTCCGCT GCCCTCATCG TTCGTGAGAA CGGCCAGGTC AAGCCAGTCC AGTTCAAGGA CCTCCTCGAC CCAGCTACAG GCCGTGTCCG CACACGCCTT GTCGATGTTA CATCACAGTC ATTCAAGGTC GCTCGTGTCT ACATGTGGCG CATGAGCAAG AAGGACTACG AGAACAAGGA TCTCGTCGCC CGCGTCGCTG CTGCCGGCAA GATGACACCA GAGGCCTTCA CAGAGAAGTT CGCTCACCTT ACAGATGTTG TCGTCGAA
Details for TrvaA.00429.d.B1.PW39248
PURIFICATION DATe: 12/15/2023
CONCENTRATION: 25mg/ml
OBSERVED MW: 48kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol, 2 mM DTT, 0.025% Azide
EXPRESSION HOST: BL 21 (DE3) Rosetta
VIAL COUNT (approx.): 15
VIAL VOLUME: 110µl
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MAHHHHHHMS TEAPVLGILC GGGPAPGLNG VIAGATLYAL RLGWKVIGFM EGFKYLCTGD VDVVKAHTID LTYDIVSRIH FQGGTIIQTS RANPRKSPEL QENVRKCLRA LKVRYFLTIG GDDTASSAVS VASGMNGNEI SVISCPKTID NDLPLPADQS TFGFHTARSL GMEIIRNLMV DSKSAPRWFL VEAMGRSAGH LALGMAEASG AHLCLIPEEF KQDEIEFEDV VELVEATILK RLAYGKNYGV CVLAEGLVSK MSKKALYKLF GNREPPTDPH GHILLDDAEL ARSLSEELLK RLGNLGIRIT PKKIGYELRC ADPVAFDAVY TRELGYGAID AFLNGHSAAL IVRENGQVKP VQFKDLLDPA TGRVRTRLVD VTSQSFKVAR VYMWRMSKKD YENKDLVARV AAAGKMTPEA FTEKFAHLTD VVVE
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAGCA CTGAAGCTCC TGTTCTCGGT ATCCTCTGCG GTGGTGGTCC AGCCCCAGGC CTCAATGGTG TCATCGCTGG CGCTACACTT TACGCCCTTA GACTTGGCTG GAAGGTCATC GGCTTCATGG AAGGTTTCAA GTACCTTTGC ACAGGCGATG TTGATGTCGT CAAGGCCCAC ACAATCGACC TCACATACGA TATCGTTTCT CGTATCCACT TCCAGGGTGG CACAATCATC CAAACATCCC GTGCTAACCC ACGTAAGTCC CCAGAACTCC AAGAGAACGT CCGCAAGTGC CTCCGCGCTC TCAAGGTTCG TTACTTCCTT ACAATCGGTG GTGATGATAC AGCTTCATCC GCTGTTTCCG TCGCTTCCGG CATGAACGGC AACGAGATCT CTGTCATCTC CTGCCCAAAG ACAATCGATA ACGATCTTCC ACTCCCAGCT GACCAGTCCA CATTCGGCTT CCACACAGCT CGCTCCCTTG GTATGGAAAT CATCCGCAAC CTCATGGTTG ACTCCAAGTC TGCCCCACGT TGGTTCCTTG TCGAAGCTAT GGGCCGCTCT GCTGGTCACC TTGCTCTCGG CATGGCTGAA GCTTCTGGTG CCCACCTTTG CCTCATCCCA GAAGAATTCA AGCAGGACGA AATCGAATTC GAAGATGTTG TTGAACTCGT CGAAGCTACA ATCCTTAAGC GCCTTGCTTA CGGAAAGAAC TACGGTGTCT GCGTTCTTGC AGAAGGCCTC GTTTCCAAGA TGTCCAAGAA GGCTCTCTAC AAGCTCTTCG GCAACCGCGA ACCACCAACA GATCCACACG GCCACATCCT CCTCGATGAT GCTGAACTCG CTCGTTCTCT CTCCGAGGAA CTCCTCAAGC GCCTTGGCAA CCTCGGCATC AGAATCACAC CAAAGAAGAT CGGTTACGAA CTTCGTTGCG CTGATCCAGT CGCCTTCGAT GCTGTCTACA CACGTGAGCT CGGCTACGGT GCTATCGATG CCTTCCTCAA CGGCCACTCC GCTGCCCTCA TCGTTCGTGA GAACGGCCAG GTCAAGCCAG TCCAGTTCAA GGACCTCCTC GACCCAGCTA CAGGCCGTGT CCGCACACGC CTTGTCGATG TTACATCACA GTCATTCAAG GTCGCTCGTG TCTACATGTG GCGCATGAGC AAGAAGGACT ACGAGAACAA GGATCTCGTC GCCCGCGTCG CTGCTGCCGG CAAGATGACA CCAGAGGCCT TCACAGAGAA GTTCGCTCAC CTTACAGATG TTGTCGTCGA ATGAGTAAGA TAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAATTC TTGAAGACGA AAGGGCCTCG TGATACGCCT ATTTTTATAG GTTAATGTCA TGATAATAAT GGTTTCTTAG ACGTCAGGTG GCACTTTTCG GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTGT TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGC AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT GATTAAGCAT TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC GTGAAGCGAT TCACAGATGT CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG AAGCGTTAAT GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT TTTCCTGTTT GGTCACTGAT GCCTCCGTGT AAGGGGGATT TCTGTTCATG GGGGTAATGA TACCGATGAA ACGAGAGAGG ATGCTCACGA TACGGGTTAC TGATGATGAA CATGCCCGGT TACTGGAACG TTGTGAGGGT AAACAACTGG CGGTATGGAT GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG CGCTTCGTTA ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC TGCGATGCAG ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT CCAGACTTTA CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG GTCGCAGACG TTTTGCAGCA GCAGTCGCTT CACGTTCGCT CGCGTATCGG TGATTCATTC TGCTAACCAG TAAGGCAACC CCGCCAGCCT AGCCGGGTCC TCAACGACAG GAGCACGATC ATGCGCACCC GTGGCCAGGA CCCAACGCTG CCCGAGATGC GCCGCGTGCG GCTGCTGGAG ATGGCGGACG CGATGGATAT GTTCTGCCAA GGGTTGGTTT GCGCATTCAC AGTTCTCCGC AAGAATTGAT TGGCTCCAAT TCTTGGAGTG GTGAATCCGT TAGCGAGGTG CCGCCGGCTT CCATTCAGGT CGAGGTGGCC CGGCTCCATG CACCGCGACG CAACGCGGGG AGGCAGACAA GGTATAGGGC GGCGCCTACA ATCCATGCCA ACCCGTTCCA TGTGCTCGCC GAGGCGGCAT AAATCGCCGT GACGATCAGC GGTCCAGTGA TCGAAGTTAG GCTGGTAAGA GCCGCGAGCG ATCCTTGAAG CTGTCCCTGA TGGTCGTCAT CTACCTGCCT GGACAGCATG GCCTGCAACG CGGGCATCCC GATGCCGCCG GAAGCGAGAA GAATCATAAT GGGGAAGGCC ATCCAGCCTC GCGTCGCGAA CGCCAGCAAG ACGTAGCCCA GCGCGTCGGC CGCCATGCCG GCGATAATGG CCTGCTTCTC GCCGAAACGT TTGGTGGCGG GACCAGTGAC GAAGGCTTGA GCGAGGGCGT GCAAGATTCC GAATACCGCA AGCGACAGGC CGATCATCGT CGCGCTCCAG CGAAAGCGGT CCTCGCCGAA AATGACCCAG AGCGCTGCCG GCACCTGTCC TACGAGTTGC ATGATAAAGA AGACAGTCAT AAGTGCGGCG ACGATAGTCA TGCCCCGCGC CCACCGGAAG GAGCTGACTG GGTTGAAGGC TCTCAAGGGC ATCGGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAAT