TrvaA.00425.a

Elongation factor 1-alpha

CENTER ID: TrvaA.00425.a
ORGANISM: Trichomonas vaginalis ATCC PRA-98 / G3
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: unclassified
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
TrvaA.00425.a.B1.GE36544 full length 1 437
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: TrichDB:TVAG_067400
RefSeq: XP_001325483.1
RefSeq: XP_001328958.1
UniProt: A2DSF6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MGKEKEHINI VVIGHVDAGK STTTGHLIYK CGGLDKRKLA AIEKEAEQLG KSSFKYAFVM DSLKAERERG ITIDISLWKF EGQKFSFTII DAPGHRDFIK NMITGTSQAD AAILVIDSTL GGFEAGIAEQ GQTREHALLA FTLGIKQVIV AVNKMDDKTV NYNKARFDEI TAEMTRILTG IGYKPEMFRF VPISGWAGDN MTEKSPNMPW YNGPYLLEAL DSLQPPKRPF DKPLRLPLQD VYKINGIGTV PVGRVESGTM KPGMIVNFAP STVTAEVKSI EMHHESLPEA LPGDNIGFNV KNVSTADVKR GYVVGDTKRD PPVECASFTA QMIISNHPGK IHAGYQPVFD CHTAHIACKF DKLIQRIDRR HGKKATENPE YIQKDDAAIV EVVPSKPLVV ESFQEYPPLG RFAIRDMKQT VAVGVIRSVN KKPNPIK
NT Sequence
atgggtaagg agaaagagca tatcaatatt gtcgttatcg gacacgtcga tgcaggtaag tccacaacaa caggccacct catctacaag tgcggcggtc tcgataagcg taaacttgct gccatcgaaa aggaagctga acaactcggc aagtcctcct tcaagtacgc tttcgttatg gactccctca aggctgaacg tgagcgtggt atcacaatcg atatctccct ctggaagttc gaaggccaga agttctcctt caccatcatc gatgccccag gacaccgtga cttcatcaag aacatgatca caggcacatc ccaggctgat gctgctatcc ttgtcatcga ctccacactc ggtggcttcg aagctggtat cgctgaacaa ggccagacac gtgaacacgc tcttcttgcc ttcacccttg gtatcaagca ggtcattgtc gccgtcaaca agatggatga caagacagtc aattacaaca aggctcgttt cgacgaaatc acagcagaaa tgacacgtat ccttacaggc atcggctaca agccagaaat gttccgcttc gtcccaatct ccggctgggc tggcgacaac atgacagaga agtctccaaa catgccatgg tacaatggcc cataccttct tgaagccctc gattcccttc agccaccaaa gcgcccattc gacaagccac tccgtcttcc actccaggat gtctacaaga tcaacggtat cggtacagtt ccagtcggcc gtgtcgaatc cggcacaatg aagccaggca tgatcgttaa cttcgcccca tccacagtta cagctgaagt taagtccatc gaaatgcacc acgaatccct tccagaggct cttccaggtg acaacatcgg cttcaacgtc aagaacgttt ccacagctga tgtcaagcgt ggctacgtcg ttggtgatac aaagcgtgac ccaccagtcg aatgcgcttc cttcacagct cagatgatca tctccaacca cccaggcaag atccacgccg gctaccagcc agttttcgac tgccacacag cccacatcgc ctgcaagttc gacaagctca tccagcgtat cgatcgtcgc cacggcaaga aggctacaga gaacccagaa tacattcaga aggatgatgc cgctatcgtc gaggttgtcc catccaagcc actcgtcgtc gagtccttcc aggagtaccc accactcggc cgtttcgcca tccgtgatat gaagcagaca gtcgctgtcg gcgttatccg ttccgtcaac aagaagccaa acccaatcaa gtaa
Details for TrvaA.00425.a.B1.GE36544
HARVESTED ON: 1/9/2013
SEQUENCED ON: 1/7/2013
EXPECTED MW: 49kDa
OBSERVED MW: 49kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with sequence variation
PERCENT IDENTITY: 100
PERCENT COVERAGE: 82
Validated AA Sequence
MDSLKAERER GITIDISLWK FEGQKFSFTI IDAPGHRDFI KNMITGTSQA DAAILVIDST LGGFEAGIAE QGQTREHALL AFTLGIKQVI VAVNKMDDKT VNYNKARFDE ITAEMTRILT GIGYKPEMFR FVPISGWAGD NMTEKSPNMP WYNGPYLLEA LDSLQPPKRP FDKPLRLPLQ DVYKINGIGT VPVGRVESGT MKPGMIVNFA PSTVTAEVKS IEMHHESLPE ALPGDNIGFN VKNVSTADVK RGYVVGDTKR DPPVECASFT AQMIISNHPG KIHAGYQPVF DCHTAHIACK FDKLIQRIDR RHGKKATENP EYIQKDDAAI VEVVPSKPLV VESFQEYPPL GRFAIRDMKQ TVAVGVIRS
Validated NT Sequence
gacggaacgg ataacgccga cagcgactgt ctgcttcata tcacggatgg cgaaacggcc gagtggtggg tactcctgga aggactcgac gacgagtggc ttggatggga caacctcgac gatagcggca tcatccttct gaatgtattc tgggttctct gtagccttct tgccgtggcg acgatcgata cgctggatga gcttgtcgaa cttgcaggcg atgtgagctg tgtggcagtc gaaaactggc tggtagccgg cgtggatctt gcctgggtgg ttggagatga tcatctgagc tgtgaaggaa gcgcattcga ctggtgggtc acgctttgta tcaccaacga cgtagccacg cttgacatca gctgtggaaa cgttcttgac gttgaagccg atgttgtcac ctggaagagc ctctggaagg gattcgtggt gcatttcgat ggacttaact tcagctgtaa ctgtggatgg ggcgaagtta acgatcatgc ctggcttcat tgtgccggat tcgacacggc cgactggaac tgtaccgata ccgttgatct tgtagacatc ctggagtgga agacggagtg gcttgtcgaa tgggcgcttt ggtggctgaa gggaatcgag ggcttcaaga aggtatgggc cattgtacca tggcatgttt ggagacttct ctgtcatgtt gtcgccagcc cagccggaga ttgggacgaa gcggaacatt tctggcttgt agccgatgcc tgtaaggatg cgtgtcattt cggctgtgat ttcgtcgaaa cgggccttgt tgtagttgac tgtcttgtca tccatcttgt tgacggcgac aatgacctgc ttgatgccga gtgtgaaggc aagaagagcg tgttcacgtg tctggccttg ttcagcgata ccggcttcga aaccaccgag tgtggagtcg atgacaagga tagcagcatc agcctgggat gtgcctgtga tcatgttctt gatgaagtca cggtgtcctg gggcatcaat gattgtgaag gagaacttct ggccttcgaa cttccagagg gagatatcga ttgtgatacc acgctcacgt tcagccttga gggagtccat aacgaaggcg tacttgaagg aggacttgcc gagttgttca gcttcttttt cgatagcagc aagtttacgc ttatcgagac cgccgcactt gtagatgagg tggcctgttg ttgtggactt gccagcatcg acgtgtccga taacgacaat attgatatgc tctttctcct ta
Expected Protein Sequence
MAHHHHHHMG KEKEHINIVV IGHVDAGKST TTGHLIYKCG GLDKRKLAAI EKEAEQLGKS SFKYAFVMDS LKAERERGIT IDISLWKFEG QKFSFTIIDA PGHRDFIKNM ITGTSQADAA ILVIDSTLGG FEAGIAEQGQ TREHALLAFT LGIKQVIVAV NKMDDKTVNY NKARFDEITA EMTRILTGIG YKPEMFRFVP ISGWAGDNMT EKSPNMPWYN GPYLLEALDS LQPPKRPFDK PLRLPLQDVY KINGIGTVPV GRVESGTMKP GMIVNFAPST VTAEVKSIEM HHESLPEALP GDNIGFNVKN VSTADVKRGY VVGDTKRDPP VECASFTAQM IISNHPGKIH AGYQPVFDCH TAHIACKFDK LIQRIDRRHG KKATENPEYI QKDDAAIVEV VPSKPLVVES FQEYPPLGRF AIRDMKQTVA VGVIRSVNKK PNPIK
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgggta aggagaaaga gcatatcaat attgtcgtta tcggacacgt cgatgcaggt aagtccacaa caacaggcca cctcatctac aagtgcggcg gtctcgataa gcgtaaactt gctgccatcg aaaaggaagc tgaacaactc ggcaagtcct ccttcaagta cgctttcgtt atggactccc tcaaggctga acgtgagcgt ggtatcacaa tcgatatctc cctctggaag ttcgaaggcc agaagttctc cttcaccatc atcgatgccc caggacaccg tgacttcatc aagaacatga tcacaggcac atcccaggct gatgctgcta tccttgtcat cgactccaca ctcggtggct tcgaagctgg tatcgctgaa caaggccaga cacgtgaaca cgctcttctt gccttcaccc ttggtatcaa gcaggtcatt gtcgccgtca acaagatgga tgacaagaca gtcaattaca acaaggctcg tttcgacgaa atcacagcag aaatgacacg tatccttaca ggcatcggct acaagccaga aatgttccgc ttcgtcccaa tctccggctg ggctggcgac aacatgacag agaagtctcc aaacatgcca tggtacaatg gcccatacct tcttgaagcc ctcgattccc ttcagccacc aaagcgccca ttcgacaagc cactccgtct tccactccag gatgtctaca agatcaacgg tatcggtaca gttccagtcg gccgtgtcga atccggcaca atgaagccag gcatgatcgt taacttcgcc ccatccacag ttacagctga agttaagtcc atcgaaatgc accacgaatc ccttccagag gctcttccag gtgacaacat cggcttcaac gtcaagaacg tttccacagc tgatgtcaag cgtggctacg tcgttggtga tacaaagcgt gacccaccag tcgaatgcgc ttccttcaca gctcagatga tcatctccaa ccacccaggc aagatccacg ccggctacca gccagttttc gactgccaca cagcccacat cgcctgcaag ttcgacaagc tcatccagcg tatcgatcgt cgccacggca agaaggctac agagaaccca gaatacattc agaaggatga tgccgctatc gtcgaggttg tcccatccaa gccactcgtc gtcgagtcct tccaggagta cccaccactc ggccgtttcg ccatccgtga tatgaagcag acagtcgctg tcggcgttat ccgttccgtc aacaagaagc caaacccaat caagtgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat