TrpaC.00084.a

Enolase (EC 4.2.1.11) (2-phospho-D-glycerate hydro-lyase) (2-phosphoglycerate dehydratase)

CENTER ID: TrpaC.00084.a
ORGANISM: Treponema pallidum subsp. pallidum Nichols
ASSOCIATED DISEASE: Syphilis
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

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Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
TrpaC.00084.a.B1.PW39560 full length 1 432

External Resources

RESOURCE REFERENCE ID
UniProt: P74934

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSDIACIEAR EIIDSRGNPT VEVDVSLSDG SFGRACVPSG ASTGEFEALE MRDGDKERYN GKGVLKAVGT VNTLIADTLE GMDALNQGEI DHAMRNLDGT DNKSKLGANA MLGVSMACAR AAADFLGVPL YRYLGGVHTF RMPVPMANII NGGKHSDNKI DFQEFMVMPI GAASMREAVR MTAEVFHALK GLLAADGKAT SVGDEGGFAP DLDNEQALEY IMKAIAKAGL APRKDVCIAL DCASSELFDE GDRRGYKFWK SNPGKLFTAQ EMIDLYKKWI ATYPIVSIED PLDQNDWAGY VQLTKELGDK VQIVGDDFFV TNTGRLARGI KEGSCNSILI KLNQIGTVTE TVDAVRMAQN AGYAAVISHR SGETEDAFIA DLAVALETGQ IKTGSMSRSD RVAKYNQLMR IEEELGAQAR YYGAKTFERF GC
NT Sequence
gtgagtgaca ttgcatgcat tgaagcgcgc gagatcatcg attctcgggg aaaccccact gtcgaagtgg atgtctcgct cagtgacggt agctttggcc gtgcgtgtgt tccttcaggg gcgtctaccg gagagttcga ggcgctcgaa atgcgtgatg gagacaagga gcgctataac ggtaagggtg ttctcaaggc cgttggaact gtcaacacgc tcattgctga tacgctcgaa ggtatggatg cgctcaacca gggtgagata gatcacgcga tgcggaatct ggacggcact gacaataagt caaagcttgg tgcgaatgct atgcttggcg tctccatggc ttgtgcgcgt gctgctgcag actttctggg tgttcctctc taccgttacc tcggtggtgt gcacactttt cgcatgccgg tccctatggc caacattatc aatgggggga agcattctga taataaaatt gactttcaag agtttatggt tatgcccatc ggcgctgcgt ccatgcgcga agcggtgcgc atgaccgcag aggtgttcca cgcgttaaag ggcctgcttg ccgccgacgg caaagctacg tcagttggtg atgaaggtgg ttttgcgcct gatttggata acgagcaagc ccttgagtac attatgaaag cgatcgcaaa agccggcctt gcgccgcgga aggatgtgtg tattgcgctt gactgtgcgt cttctgagtt attcgatgaa ggagatcgga gagggtacaa gttttggaag tcgaatccgg ggaagctgtt cacagcacag gaaatgattg atctgtacaa gaagtggatt gctacgtatc cgattgtgtc tatcgaggat ccgcttgacc aaaacgactg ggcaggttac gtgcagctca cgaaagagtt gggagacaaa gtccaaattg tgggagatga tttttttgta accaacactg ggcgtctggc ccgaggtatc aaagaaggat cgtgcaattc gattctgata aaacttaatc agattggcac ggtcacagaa actgtcgacg cggtgcgtat ggctcagaac gcaggctacg cggcggtgat ttcacatcgc tcgggtgaga cagaggatgc gtttatcgca gacttagcag tggcacttga gacaggtcaa atcaaaaccg gctcgatgag tcgcagtgac cgggtggcga agtataacca gttgatgcgc attgaggaag agttgggtgc acaggcgcgc tactacgggg caaagacctt tgagcggttt ggttgttag
Details for TrpaC.00084.a.B1.PW39560
PURIFICATION DATe: 4/14/2026
CONCENTRATION: 24.2mg/ml
OBSERVED MW: 48kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol, 2 mM DTT, 0.025% Azide
EXPRESSION HOST: BL 21 (DE3) Rosetta
VIAL COUNT (approx.): 5
VIAL VOLUME: 200µl
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MAHHHHHHMS DIACIEAREI IDSRGNPTVE VDVSLSDGSF GRACVPSGAS TGEFEALEMR DGDKERYNGK GVLKAVGTVN TLIADTLEGM DALNQGEIDH AMRNLDGTDN KSKLGANAML GVSMACARAA ADFLGVPLYR YLGGVHTFRM PVPMANIING GKHSDNKIDF QEFMVMPIGA ASMREAVRMT AEVFHALKGL LAADGKATSV GDEGGFAPDL DNEQALEYIM KAIAKAGLAP RKDVCIALDC ASSELFDEGD RRGYKFWKSN PGKLFTAQEM IDLYKKWIAT YPIVSIEDPL DQNDWAGYVQ LTKELGDKVQ IVGDDFFVTN TGRLARGIKE GSCNSILIKL NQIGTVTETV DAVRMAQNAG YAAVISHRSG ETEDAFIADL AVALETGQIK TGSMSRSDRV AKYNQLMRIE EELGAQARYY GAKTFERFGC
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATGTGAGTG ACATTGCATG CATTGAAGCG CGCGAGATCA TCGATTCTCG GGGAAACCCC ACTGTCGAAG TGGATGTCTC GCTCAGTGAC GGTAGCTTTG GCCGTGCGTG TGTTCCTTCA GGGGCGTCTA CCGGAGAGTT CGAGGCGCTC GAAATGCGTG ATGGAGACAA GGAGCGCTAT AACGGTAAGG GTGTTCTCAA GGCCGTTGGA ACTGTCAACA CGCTCATTGC TGATACGCTC GAAGGTATGG ATGCGCTCAA CCAGGGTGAG ATAGATCACG CGATGCGGAA TCTGGACGGC ACTGACAATA AGTCAAAGCT TGGTGCGAAT GCTATGCTTG GCGTCTCCAT GGCTTGTGCG CGTGCTGCTG CAGACTTTCT GGGTGTTCCT CTCTACCGTT ACCTCGGTGG TGTGCACACT TTTCGCATGC CGGTCCCTAT GGCCAACATT ATCAATGGGG GGAAGCATTC TGATAATAAA ATTGACTTTC AAGAGTTTAT GGTTATGCCC ATCGGCGCTG CGTCCATGCG CGAAGCGGTG CGCATGACCG CAGAGGTGTT CCACGCGTTA AAGGGCCTGC TTGCCGCCGA CGGCAAAGCT ACGTCAGTTG GTGATGAAGG TGGTTTTGCG CCTGATTTGG ATAACGAGCA AGCCCTTGAG TACATTATGA AAGCGATCGC AAAAGCCGGC CTTGCGCCGC GGAAGGATGT GTGTATTGCG CTTGACTGTG CGTCTTCTGA GTTATTCGAT GAAGGAGATC GGAGAGGGTA CAAGTTTTGG AAGTCGAATC CGGGGAAGCT GTTCACAGCA CAGGAAATGA TTGATCTGTA CAAGAAGTGG ATTGCTACGT ATCCGATTGT GTCTATCGAG GATCCGCTTG ACCAAAACGA CTGGGCAGGT TACGTGCAGC TCACGAAAGA GTTGGGAGAC AAAGTCCAAA TTGTGGGAGA TGATTTTTTT GTAACCAACA CTGGGCGTCT GGCCCGAGGT ATCAAAGAAG GATCGTGCAA TTCGATTCTG ATAAAACTTA ATCAGATTGG CACGGTCACA GAAACTGTCG ACGCGGTGCG TATGGCTCAG AACGCAGGCT ACGCGGCGGT GATTTCACAT CGCTCGGGTG AGACAGAGGA TGCGTTTATC GCAGACTTAG CAGTGGCACT TGAGACAGGT CAAATCAAAA CCGGCTCGAT GAGTCGCAGT GACCGGGTGG CGAAGTATAA CCAGTTGATG CGCATTGAGG AAGAGTTGGG TGCACAGGCG CGCTACTACG GGGCAAAGAC CTTTGAGCGG TTTGGTTGTT GAGTAAGATA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAATTCTT GAAGACGAAA GGGCCTCGTG ATACGCCTAT TTTTATAGGT TAATGTCATG ATAATAATGG TTTCTTAGAC GTCAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG ATGCTGAAGA TCAGTTGGGT GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA AGATCCTTGA GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC TGCTATGTGG CGCGGTATTA TCCCGTGTTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA TACACTATTC TCAGAATGAC TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG ATGGCATGAC AGTAAGAGAA TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG CCAACTTACT TCTGACAACG ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA TGGGGGATCA TGTAACTCGC CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA ACGACGAGCG TGACACCACG ATGCCTGCAG CAATGGCAAC AACGTTGCGC AAACTATTAA CTGGCGAACT ACTTACTCTA GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA AAGTTGCAGG ACCACTTCTG CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC CCTCCCGTAT CGTAGTTATC TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA GACAGATCGC TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TCCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC TGATGCGGTA TTTTCTCCTT ACGCATCTGT GCGGTATTTC ACACCGCATA TATGGTGCAC TCTCAGTACA ATCTGCTCTG ATGCCGCATA GTTAAGCCAG TATACACTCC GCTATCGCTA CGTGACTGGG TCATGGCTGC GCCCCGACAC CCGCCAACAC CCGCTGACGC GCCCTGACGG GCTTGTCTGC TCCCGGCATC CGCTTACAGA CAAGCTGTGA CCGTCTCCGG GAGCTGCATG TGTCAGAGGT TTTCACCGTC ATCACCGAAA CGCGCGAGGC AGCTGCGGTA AAGCTCATCA GCGTGGTCGT GAAGCGATTC ACAGATGTCT GCCTGTTCAT CCGCGTCCAG CTCGTTGAGT TTCTCCAGAA GCGTTAATGT CTGGCTTCTG ATAAAGCGGG CCATGTTAAG GGCGGTTTTT TCCTGTTTGG TCACTGATGC CTCCGTGTAA GGGGGATTTC TGTTCATGGG GGTAATGATA CCGATGAAAC GAGAGAGGAT GCTCACGATA CGGGTTACTG ATGATGAACA TGCCCGGTTA CTGGAACGTT GTGAGGGTAA ACAACTGGCG GTATGGATGC GGCGGGACCA GAGAAAAATC ACTCAGGGTC AATGCCAGCG CTTCGTTAAT ACAGATGTAG GTGTTCCACA GGGTAGCCAG CAGCATCCTG CGATGCAGAT CCGGAACATA ATGGTGCAGG GCGCTGACTT CCGCGTTTCC AGACTTTACG AAACACGGAA ACCGAAGACC ATTCATGTTG TTGCTCAGGT CGCAGACGTT TTGCAGCAGC AGTCGCTTCA CGTTCGCTCG CGTATCGGTG ATTCATTCTG CTAACCAGTA AGGCAACCCC GCCAGCCTAG CCGGGTCCTC AACGACAGGA GCACGATCAT GCGCACCCGT GGCCAGGACC CAACGCTGCC CGAGATGCGC CGCGTGCGGC TGCTGGAGAT GGCGGACGCG ATGGATATGT TCTGCCAAGG GTTGGTTTGC GCATTCACAG TTCTCCGCAA GAATTGATTG GCTCCAATTC TTGGAGTGGT GAATCCGTTA GCGAGGTGCC GCCGGCTTCC ATTCAGGTCG AGGTGGCCCG GCTCCATGCA CCGCGACGCA ACGCGGGGAG GCAGACAAGG TATAGGGCGG CGCCTACAAT CCATGCCAAC CCGTTCCATG TGCTCGCCGA GGCGGCATAA ATCGCCGTGA CGATCAGCGG TCCAGTGATC GAAGTTAGGC TGGTAAGAGC CGCGAGCGAT CCTTGAAGCT GTCCCTGATG GTCGTCATCT ACCTGCCTGG ACAGCATGGC CTGCAACGCG GGCATCCCGA TGCCGCCGGA AGCGAGAAGA ATCATAATGG GGAAGGCCAT CCAGCCTCGC GTCGCGAACG CCAGCAAGAC GTAGCCCAGC GCGTCGGCCG CCATGCCGGC GATAATGGCC TGCTTCTCGC CGAAACGTTT GGTGGCGGGA CCAGTGACGA AGGCTTGAGC GAGGGCGTGC AAGATTCCGA ATACCGCAAG CGACAGGCCG ATCATCGTCG CGCTCCAGCG AAAGCGGTCC TCGCCGAAAA TGACCCAGAG CGCTGCCGGC ACCTGTCCTA CGAGTTGCAT GATAAAGAAG ACAGTCATAA GTGCGGCGAC GATAGTCATG CCCCGCGCCC ACCGGAAGGA GCTGACTGGG TTGAAGGCTC TCAAGGGCAT CGGTCGACGC TCTCCCTTAT GCGACTCCTG CATTAGGAAG CAGCCCAGTA GTAGGTTGAG GCCGTTGAGC ACCGCCGCCG CAAGGAATGG TGCATGCAAG GAGATGGCGC CCAACAGTCC CCCGGCCACG GGGCCTGCCA CCATACCCAC GCCGAAACAA GCGCTCATGA GCCCGAAGTG GCGAGCCCGA TCTTCCCCAT CGGTGATGTC GGCGATATAG GCGCCAGCAA CCGCACCTGT GGCGCCGGTG ATGCCGGCCA CGATGCGTCC GGCGTAGAGG ATCGAGATCT CGATCCCGCG AAAT