TrpaA.17227.d

TprKSea81-4C122

CENTER ID: TrpaA.17227.d
ORGANISM: Treponema pallidum subsp. pallidum
ASSOCIATED DISEASE: Syphilis
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
TrpaA.17227.d.A2.GE29916 selected domain 55 503
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
External Resources
RESOURCE REFERENCE ID
OrthoMCL: OG5_152018
UniProt: Q9L8T2
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MIDPSATSRY GSPRLVSNGF RHRRKVVYQR VGHRRFSLIF FFVVVLGRSP RLWAQVSFTP DIEGYAELAW GIASDGGAQP LKHGFKTTTD FKIVFPIVAK KDFKYRGEGN VYAEINVKAL KLSLESNGGA KFDTKGSAKT IEATLHCYGA YLTIGKNPDF KSTFAVLWEP WTANGDYKSK GDKPVYEPGF EGAGGKLGYK QTDIAGTGLT FDIAFKFASN TDWEGKDSQG KAPAGTPSKY GLGGDILFGW ERTREDGVQE YIKVELTGNS TLSSGYAPAP ANNAILWDVG AKVSMKLWGL CALAATDVGH KKNGANGDIG ADALLTLGYR WFSAGGYFAS QASNVFQGVF LTTPMQKDDC AAYIKLETKG SDPDTSFLEG LDLGVDVRTY MPVHWKTRAP ADIHFPVYGK VWGSYRHDMG EYGWVKVYAN LYGGTNKKND AAATKWSKEY CGYYECGVVV SPLEKVEIRL SWEQGKLQEN SNVVIEKNVT ERWQFVGACR LIW
NT Sequence
atgattgacc catctgccac ttcccggtat ggttccccac gtttagttag taatggtttt cggcatcgga gaaaagtggt gtatcagcgg gtagggcaca ggcgattttc tctcattttc tttttcgttg tggttctggg gcggtccccg cggctgtggg ctcaggtttc gttcaccccg gatattgaag gctatgcgga gctggcctgg ggcattgcat ccgatggtgg cgcccaaccc ctcaagcatg gatttaagac tactactgat tttaagattg tgttccccat tgtggcaaag aaggatttca agtaccgcgg tgaggggaat gtctatgcgg aaattaatgt taaagcgttg aagttgagtt tagagtcaaa tggtggagca aagtttgaca cgaagggttc tgcaaagacg atagaggcaa ccctgcactg ttatggggcc tacctgacca ttgggaagaa tcctgatttt aagtcaacgt ttgctgtttt gtgggagccg tggaccgcga atggggatta taagtctaag ggagataagc cggtgtatga gccggggttt gagggagccg ggggaaagtt agggtataaa cagactgaca tcgccggcac ggggctcacg tttgatattg cgtttaagtt tgcgtctaac accgactggg agggcaaaga cagtcagggc aaggccccag caggaactcc cagcaagtat ggattggggg gagatatttt gttcggctgg gagcgtacgc gtgaagatgg cgtgcaggaa tacattaaag tggagctcac cggcaactcc acactgtcta gcggctatgc cccagcccca gccaacaacg ccatcttatg ggatgtcggg gctaaggtga gtatgaagct ttggggtctg tgtgctctgg ctgctacaga cgtggggcat aagaaaaacg gagcgaatgg cgacataggc gcagatgcgt tgttgacgtt ggggtatcgt tggttctcgg cgggaggata tttcgcatcg caggccagca atgtatttca gggtgtcttt ctcaccacac ccatgcagaa ggacgactgt gctgcctata ttaagctcga aaccaagggg tctgatcctg atacttcttt ccttgagggt cttgatttgg gtgttgatgt gcgtacgtac atgcccgtcc attggaaaac ccgagcccca gccgacatcc acttcccggt gtatggaaaa gtctggggtt cgtatcgtca tgatatgggt gagtatggtt gggttaaagt gtatgcaaac ttgtacggcg gtacgaacaa gaaaaacgat gctgctgcta cgaagtggag caaggaatat tgtgggtatt acgagtgtgg ggtagtggtc agtccgttag agaaggtgga gattcggctg agctgggagc aaggcaagct acaagagaac agcaatgtag tgatagagaa gaacgtgacg gagcgttggc aattcgtagg ggcatgtcgc ttgatttggt ag
Details for TrpaA.17227.d.A2.GE29916
HARVESTED ON: 7/13/2010
SEQUENCED ON: 8/18/2010
EXPECTED MW: 51kDa
OBSERVED MW: 50kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 96
PERCENT COVERAGE: 95
Validated AA Sequence
MQVSFTPDIE GYAELAWGIA SDGGXQPLKH GFKTTTDFXI VFPIVAKKDF KYRGEGNVYA EINVKALKLS LXSNGGAKFD TKGSAXTIEA TLHCYGAYLT IGXNPDFKST FAVLWEPWTA NGDYKSKGDK PVYXPGFEGA GGKLGYXQTD IAGTGLTFDI AFKFASNIDW EGKDSQGKAP AGTPSKYGLG GDILFGWERT REDGVQEYIK VELTGNSTLS SGYAPAPANN AILWDVGAKV SMKLWGLCAL AATDVGHKKN GANGDIGADA LLTLGYRWFS AGGYFASQAS NVFQGVFLTT PMQKEDCAAY IKLETKGSDP DTSFLEGLDL GVDVRTYMPV HAQARAPADI HFPVYGKVWG SYRHDMGEYG WVKVYANLYG GTNKQAAAVP ATKWSKEYCG YYECGVVVSP LEKVEIRLSW EQGKLQENSN VVIEKNVTER WQFVGACRLI
Validated NT Sequence
ttattaaatc aagcgacatg cccctacgaa ttgccaacgc tccgtcacgt tcttctctat cactacattg ctgttctctt gtagcttgcc ttgctcccag ctcagccgaa tctccacctt ctctaacgga ctgaccacta ccccacactc gtaataccca caatattcct tgctccactt cgtagcagga accgcagcag cttgcttgtt cgtaccgccg tacaagtttg catacacttt aacccaacca tactcaccca tatcatgacg atacgaaccc cagacttttc catacaccgg gaagtggatg tcggctgggg ctcgggcttg ggcgtggacg ggcatgtacg tacgcacatc aacacccaaa tcaagaccct caaggaaaga agtatcagga tcagacccct tggtttcgag cttaatatag gcagcacagt cctccttctg catgggtgtg gtgagaaaga caccctgaaa tacattgctg gcctgcgatg cgaaatatcc tcccgccgag aaccaacgat accccaacgt caacaacgca tctgcgccta tgtcgccatt cgctccgttt ttcttatgcc ccacgtctgt agcagccaga gcacacagac cccaaagctt catactcacc ttagccccga catcccataa gatggcgttg ttggctgggg ctggggcata gccgctagac agtgtggagt tgccggtgag ctccacttta atgtattcct gcacgccatc ttcacgcgta cgctcccagc cgaacaaaat atctcccccc aatccatact tgctgggagt tcctgctggg gccttgccct gactgtcttt gccctcccag tcgatgttag acgcaaactt aaacgcaata tcaaacgtga gccccgtgcc ggcgatgtca gtctgnttat accctaactt tcccccggct ccctcaaacc ccggntcata caccggctta tctcccttag acttataatc cccattcgcg gtccacggct cccacaaaac agcaaacgtt gacttaaaat caggattntt nccaatggtc aggtaggccc cataacagtg cagggttgcc tctatngtnt ttgcagaacc cttngtgtca aactttgctc caccatttga ctntaaactc aacttcaacg ctttaacatt aatttccgca tagacattcc cctcaccgcg gtacttgaaa tccttctttg ctacaatggg gaacacaatn ttaaaatcag tagtagtctt aaatccatgc ttgaggggtt gggngccacc atcggatgca atgccccagg ccagctccgc atagccttca atatccgggg tgaacgaaac ctgcatcgaa ccaggaccct gggtctgagc ttccagggta c
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMQVSFTPDI EGYAELAWGI ASDGGAQPLK HGFKTTTDFK IVFPIVAKKD FKYRGEGNVY AEINVKALKL SLESNGGAKF DTKGSAKTIE ATLHCYGAYL TIGKNPDFKS TFAVLWEPWT ANGDYKSKGD KPVYEPGFEG AGGKLGYKQT DIAGTGLTFD IAFKFASNTD WEGKDSQGKA PAGTPSKYGL GGDILFGWER TREDGVQEYI KVELTGNSTL SSGYAPAPAN NAILWDVGAK VSMKLWGLCA LAATDVGHKK NGANGDIGAD ALLTLGYRWF SAGGYFASQA SNVFQGVFLT TPMQKDDCAA YIKLETKGSD PDTSFLEGLD LGVDVRTYMP VHWKTRAPAD IHFPVYGKVW GSYRHDMGEY GWVKVYANLY GGTNKKNDAA ATKWSKEYCG YYECGVVVSP LEKVEIRLSW EQGKLQENSN VVIEKNVTER WQFVGACRLI
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcaggtttcg ttcaccccgg atattgaagg ctatgcggag ctggcctggg gcattgcatc cgatggtggc gcccaacccc tcaagcatgg atttaagact actactgatt ttaagattgt gttccccatt gtggcaaaga aggatttcaa gtaccgcggt gaggggaatg tctatgcgga aattaatgtt aaagcgttga agttgagttt agagtcaaat ggtggagcaa agtttgacac gaagggttct gcaaagacga tagaggcaac cctgcactgt tatggggcct acctgaccat tgggaagaat cctgatttta agtcaacgtt tgctgttttg tgggagccgt ggaccgcgaa tggggattat aagtctaagg gagataagcc ggtgtatgag ccggggtttg agggagccgg gggaaagtta gggtataaac agactgacat cgccggcacg gggctcacgt ttgatattgc gtttaagttt gcgtctaaca ccgactggga gggcaaagac agtcagggca aggccccagc aggaactccc agcaagtatg gattgggggg agatattttg ttcggctggg agcgtacgcg tgaagatggc gtgcaggaat acattaaagt ggagctcacc ggcaactcca cactgtctag cggctatgcc ccagccccag ccaacaacgc catcttatgg gatgtcgggg ctaaggtgag tatgaagctt tggggtctgt gtgctctggc tgctacagac gtggggcata agaaaaacgg agcgaatggc gacataggcg cagatgcgtt gttgacgttg gggtatcgtt ggttctcggc gggaggatat ttcgcatcgc aggccagcaa tgtatttcag ggtgtctttc tcaccacacc catgcagaag gacgactgtg ctgcctatat taagctcgaa accaaggggt ctgatcctga tacttctttc cttgagggtc ttgatttggg tgttgatgtg cgtacgtaca tgcccgtcca ttggaaaacc cgagccccag ccgacatcca cttcccggtg tatggaaaag tctggggttc gtatcgtcat gatatgggtg agtatggttg ggttaaagtg tatgcaaact tgtacggcgg tacgaacaag aaaaacgatg ctgctgctac gaagtggagc aaggaatatt gtgggtatta cgagtgtggg gtagtggtca gtccgttaga gaaggtggag attcggctga gctgggagca aggcaagcta caagagaaca gcaatgtagt gatagagaag aacgtgacgg agcgttggca attcgtaggg gcatgtcgct tgattaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa