SSGCID: Target Page

TrpaA.17227.c

TprKBal73-1Clone134

CENTER ID:	TrpaA.17227.c
ORGANISM:	Treponema pallidum subsp. pallidum
ASSOCIATED DISEASE:	Syphilis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
TrpaA.17227.c.A1.GE29913	full length		1	515
TrpaA.17227.c.A2.GE29914	selected domain		55	515

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
UniProt:	Q9L8T0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MIDPSATSRY GSPRLVSNGF RHRRKVVYQR VGHRRFSLIF FFVVVLGRSP RLWAQVSFTP DIEGYAELAW GIASEDGSAG NLKHGFKTTT DFKIVFPIVA KKDFKYRGEG NVYAEINVKA LKLSLESNGG AKFDTKGSAK TIEATLHCYG AYLTIGKNPD FKSTFAVLWE PWTANGDYKS KGDKPVYEPG FEGAGGKLGY KQTDIAGTGL TFDIAFKFAS NTDWEGKDSQ GNVPAGVTPS KYGLGGDILF GWERTREDGV QEYIKVELTG NSTLSSGYAQ AAGAAAAVPA AADDILWDVG AKVSMKLWGL CALAATDVGR KKDGAQGTVG ADALLTLGYR WFSAGGYFAS QASNVFQGVF LTTPMQKDDC AAYIKLETKG SDPDTSFLEG LDLGVDVRTY MPVHAQAPAP APAIYFPVYG KVWGSYRHDM GEYGWVKVYA NLYGGTNKKN DAAPATKWKA EYCGYYECGV VVSPLEKVEI RLSWEQGKLQ ENSNVVIEKN VTERWQFVGA CRLIW

NT Sequence

atgattgacc catctgccac ttcccggtat ggttccccac gtttagttag taatggtttt cggcatcgga gaaaagtggt gtatcagcgg gtagggcaca ggcgattttc tctcattttc tttttcgttg tggttctggg gcggtccccg cggctgtggg ctcaggtttc gttcaccccg gatattgaag gctatgcgga gctggcctgg ggcattgcat ccgaagatgg tagcgccgga aacctcaagc atggatttaa gactactact gattttaaga ttgtgttccc cattgtggca aagaaggatt tcaagtaccg cggtgagggg aatgtctatg cggaaattaa tgttaaagcg ttgaagttga gtttagagtc aaatggtgga gcaaagtttg acacgaaggg ttctgcaaag acgatagagg caaccctgca ctgttatggg gcctacctga ccattgggaa gaatcctgat tttaagtcaa cgtttgctgt tttgtgggag ccgtggaccg cgaatgggga ttataagtct aagggagata agccggtgta tgagccgggg tttgagggag ccgggggaaa gttagggtat aaacagactg acatcgccgg cacggggctc acgtttgata ttgcgtttaa gtttgcgtct aacaccgact gggagggcaa agacagtcag ggcaacgtcc cagcaggagt aacccccagc aagtatggat tggggggaga tattttgttc ggctgggagc gtacgcgtga agatggcgtg caggaataca ttaaagtgga gctcaccggc aactccacac tgtctagcgg ctatgcccaa gcagccggag ccgcagccgc agtgccagcc gcagccgacg acatcttatg ggatgtcggg gctaaggtga gtatgaagct ttggggtctg tgtgctctgg ctgctacaga cgtggggcgt aagaaagacg gagcgcaggg caccgtaggc gcagatgcgt tgttgacgtt ggggtatcgt tggttctcgg cgggaggata tttcgcatcg caggctagta atgtatttca gggtgtcttt ctcaccacac ccatgcagaa ggacgactgt gctgcctata ttaagctcga aaccaagggg tctgatcctg atacttcttt ccttgagggt cttgatttgg gtgttgatgt gcgtacgtac atgcccgtcc acgcccaagc cccagcccca gccccagcca tctacttccc ggtgtatgga aaagtctggg gttcgtatcg tcatgatatg ggtgagtatg gttgggttaa agtgtatgca aacttgtacg gcggtacgaa caagaaaaac gatgctgctc ctgctacgaa gtggaaggca gaatattgtg ggtattacga gtgtggggta gtggtcagtc cgttagagaa ggtggagatt cggctgagct gggagcaagg caagctacaa gagaacagca atgtagtgat agagaagaac gtgacggagc gttggcaatt cgtaggggca tgtcgcttga tttggtag

Details for TrpaA.17227.c.A1.GE29913

HARVESTED ON:	7/13/2010
SEQUENCED ON:	8/18/2010
EXPECTED MW:	58kDa
OBSERVED MW:	58kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMIDPSATSR YGSPRLVSNG FRHRRKVVYQ RVGHRRFSLI FFFVVVLGRS PRLWAQVSFT PDIEGYAELA WGIASEDGSA GNLKHGFKTT TDFKIVFPIV AKKDFKYRGE GNVYAEINVK ALKLSLESNG GAKFDTKGSA KTIEATLHCY GAYLTIGKNP DFKSTFAVLW EPWTANGDYK SKGDKPVYEP GFEGAGGKLG YKQTDIAGTG LTFDIAFKFA SNTDWEGKDS QGNVPAGVTP SKYGLGGDIL FGWERTREDG VQEYIKVELT GNSTLSSGYA QAAGAAAAVP AAADDILWDV GAKVSMKLWG LCALAATDVG RKKDGAQGTV GADALLTLGY RWFSAGGYFA SQASNVFQGV FLTTPMQKDD CAAYIKLETK GSDPDTSFLE GLDLGVDVRT YMPVHAQAPA PAPAIYFPVY GKVWGSYRHD MGEYGWVKVY ANLYGGTNKK NDAAPATKWK AEYCGYYECG VVVSPLEKVE IRLSWEQGKL QENSNVVIEK NVTERWQFVG ACRLI

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gattgaccca tctgccactt cccggtatgg ttccccacgt ttagttagta atggttttcg gcatcggaga aaagtggtgt atcagcgggt agggcacagg cgattttctc tcattttctt tttcgttgtg gttctggggc ggtccccgcg gctgtgggct caggtttcgt tcaccccgga tattgaaggc tatgcggagc tggcctgggg cattgcatcc gaagatggta gcgccggaaa cctcaagcat ggatttaaga ctactactga ttttaagatt gtgttcccca ttgtggcaaa gaaggatttc aagtaccgcg gtgaggggaa tgtctatgcg gaaattaatg ttaaagcgtt gaagttgagt ttagagtcaa atggtggagc aaagtttgac acgaagggtt ctgcaaagac gatagaggca accctgcact gttatggggc ctacctgacc attgggaaga atcctgattt taagtcaacg tttgctgttt tgtgggagcc gtggaccgcg aatggggatt ataagtctaa gggagataag ccggtgtatg agccggggtt tgagggagcc gggggaaagt tagggtataa acagactgac atcgccggca cggggctcac gtttgatatt gcgtttaagt ttgcgtctaa caccgactgg gagggcaaag acagtcaggg caacgtccca gcaggagtaa cccccagcaa gtatggattg gggggagata ttttgttcgg ctgggagcgt acgcgtgaag atggcgtgca ggaatacatt aaagtggagc tcaccggcaa ctccacactg tctagcggct atgcccaagc agccggagcc gcagccgcag tgccagccgc agccgacgac atcttatggg atgtcggggc taaggtgagt atgaagcttt ggggtctgtg tgctctggct gctacagacg tggggcgtaa gaaagacgga gcgcagggca ccgtaggcgc agatgcgttg ttgacgttgg ggtatcgttg gttctcggcg ggaggatatt tcgcatcgca ggctagtaat gtatttcagg gtgtctttct caccacaccc atgcagaagg acgactgtgc tgcctatatt aagctcgaaa ccaaggggtc tgatcctgat acttctttcc ttgagggtct tgatttgggt gttgatgtgc gtacgtacat gcccgtccac gcccaagccc cagccccagc cccagccatc tacttcccgg tgtatggaaa agtctggggt tcgtatcgtc atgatatggg tgagtatggt tgggttaaag tgtatgcaaa cttgtacggc ggtacgaaca agaaaaacga tgctgctcct gctacgaagt ggaaggcaga atattgtggg tattacgagt gtggggtagt ggtcagtccg ttagagaagg tggagattcg gctgagctgg gagcaaggca agctacaaga gaacagcaat gtagtgatag agaagaacgt gacggagcgt tggcaattcg taggggcatg tcgcttgatt aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa

Details for TrpaA.17227.c.A2.GE29914

HARVESTED ON:	7/13/2010
SEQUENCED ON:	8/20/2010
EXPECTED MW:	52kDa
OBSERVED MW:	52kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	43

Validated AA Sequence

MKLWGLCALA ATDVGRKKDG AQGTVGADAL LTLGYRWFSA GGYFASQASN VFQGVFLTTP MQKDDCAAYI KLETKGSDPD TSFLEGLDLG VDVRTYMPVH AQAPAPAPAI YFPVYGKVWG SYRHDMGEYG WVKVYANLYG GTNKKNDAAP ATKWKAEYCG YYECGVVVSP LEKVEIRLSW EQGKLQENSN VVIEKNVTER WQFVGACRLI

Validated NT Sequence

ttattaaatc aagcgacatg cccctacgaa ttgccaacgc tccgtcacgt tcttctctat cactacattg ctgttctctt gtagcttgcc ttgctcccag ctcagccgaa tctccacctt ctctaacgga ctgaccacta ccccacactc gtaataccca caatattctg ccttccactt cgtagcagga gcagcatcgt ttttcttgtt cgtaccgccg tacaagtttg catacacttt aacccaacca tactcaccca tatcatgacg atacgaaccc cagacttttc catacaccgg gaagtagatg gctggggctg gggctggggc ttgggcgtgg acgggcatgt acgtacgcac atcaacaccc aaatcaagac cctcaaggaa agaagtatca ggatcagacc ccttggtttc gagcttaata taggcagcac agtcgtcctt ctgcatgggt gtggtgagaa agacaccctg aaatacatta ctagcctgcg atgcgaaata tcctcccgcc gagaaccaac gataccccaa cgtcaacaac gcatctgcgc ctacggtgcc ctgcgctccg tctttcttac gccccacgtc tgtagcagcc agagcacaca gaccccaaag cttcatactc accttagccc cgacatccca taagatgtcg tcggctgcgg ctggcactgc ggctgcggct ccggctgctt gggcatagcc gctagacagt gtggagttgc cggtgagctc cactttaatg tattcctgca cgccatcttc acgcgtacgc tcccagcnna acaaaatatc tccccccaat ccatacttgc tgggggttac tcctgctggg acgttgccct gactgtcttt gccctcccag tnggtgttag acgcaaactt aaacgcaata tcaaacgtga gccccgtgcc ggcgatgtca gtntgtttat accctaactt tcccccggct ccctcaaacc ccggctcata caccggctta tctcccttag acttataatc cccattcgcg gtccacggct cccacaaaac agcaaacgtt gacttaaaat caggattctt cccaatggtc aggtaggccc cataacagtg cagggttgcc tctatcgtct ttgcagaacc cttcgtgtca aactttgctc caccatttga ctctaaactc aacttcaacg ctttaacatt aatttccgca tagacattcc cctcaccgcg gtacttgaaa tccttctttg ccacaatggg gaacncaatc ttaaaatcag tagtagtctn naatccatgn ttgaggtttc cggcgctacc atcttcggat gcaatgcccc aggccagctc cgcatagcct tcaatatccg gggt

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMQVSFTPDI EGYAELAWGI ASEDGSAGNL KHGFKTTTDF KIVFPIVAKK DFKYRGEGNV YAEINVKALK LSLESNGGAK FDTKGSAKTI EATLHCYGAY LTIGKNPDFK STFAVLWEPW TANGDYKSKG DKPVYEPGFE GAGGKLGYKQ TDIAGTGLTF DIAFKFASNT DWEGKDSQGN VPAGVTPSKY GLGGDILFGW ERTREDGVQE YIKVELTGNS TLSSGYAQAA GAAAAVPAAA DDILWDVGAK VSMKLWGLCA LAATDVGRKK DGAQGTVGAD ALLTLGYRWF SAGGYFASQA SNVFQGVFLT TPMQKDDCAA YIKLETKGSD PDTSFLEGLD LGVDVRTYMP VHAQAPAPAP AIYFPVYGKV WGSYRHDMGE YGWVKVYANL YGGTNKKNDA APATKWKAEY CGYYECGVVV SPLEKVEIRL SWEQGKLQEN SNVVIEKNVT ERWQFVGACR LI

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcaggtttcg ttcaccccgg atattgaagg ctatgcggag ctggcctggg gcattgcatc cgaagatggt agcgccggaa acctcaagca tggatttaag actactactg attttaagat tgtgttcccc attgtggcaa agaaggattt caagtaccgc ggtgagggga atgtctatgc ggaaattaat gttaaagcgt tgaagttgag tttagagtca aatggtggag caaagtttga cacgaagggt tctgcaaaga cgatagaggc aaccctgcac tgttatgggg cctacctgac cattgggaag aatcctgatt ttaagtcaac gtttgctgtt ttgtgggagc cgtggaccgc gaatggggat tataagtcta agggagataa gccggtgtat gagccggggt ttgagggagc cgggggaaag ttagggtata aacagactga catcgccggc acggggctca cgtttgatat tgcgtttaag tttgcgtcta acaccgactg ggagggcaaa gacagtcagg gcaacgtccc agcaggagta acccccagca agtatggatt ggggggagat attttgttcg gctgggagcg tacgcgtgaa gatggcgtgc aggaatacat taaagtggag ctcaccggca actccacact gtctagcggc tatgcccaag cagccggagc cgcagccgca gtgccagccg cagccgacga catcttatgg gatgtcgggg ctaaggtgag tatgaagctt tggggtctgt gtgctctggc tgctacagac gtggggcgta agaaagacgg agcgcagggc accgtaggcg cagatgcgtt gttgacgttg gggtatcgtt ggttctcggc gggaggatat ttcgcatcgc aggctagtaa tgtatttcag ggtgtctttc tcaccacacc catgcagaag gacgactgtg ctgcctatat taagctcgaa accaaggggt ctgatcctga tacttctttc cttgagggtc ttgatttggg tgttgatgtg cgtacgtaca tgcccgtcca cgcccaagcc ccagccccag ccccagccat ctacttcccg gtgtatggaa aagtctgggg ttcgtatcgt catgatatgg gtgagtatgg ttgggttaaa gtgtatgcaa acttgtacgg cggtacgaac aagaaaaacg atgctgctcc tgctacgaag tggaaggcag aatattgtgg gtattacgag tgtggggtag tggtcagtcc gttagagaag gtggagattc ggctgagctg ggagcaaggc aagctacaag agaacagcaa tgtagtgata gagaagaacg tgacggagcg ttggcaattc gtaggggcat gtcgcttgat taaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa