TrcrB.20383.a

Protein kinase, putative

CENTER ID: TrcrB.20383.a
ORGANISM: Trypanosoma cruzi CL Brener
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:
unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
TrcrB.20383.a.B2.GE43853 kinase domain (Jensen) 1 360
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: TriTrypDB:Tc00.1047053504089.54
UniProt: Q4DE56

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MENYLIVELI GEGSFGKVYK ARRKGTGQVV AMKFIVKKGK NDKELLNLRS EIEIMTKLDH DNIITLFEAF ETQQEFVVVM EYAQGELFEI LEDDKKLPED VVQRISKQLV QALYYLHSNR IMHRDMKPQN ILIGQNGSVK LADFGFARSM SYNTMVLTSI KGTPLYMAPE LVQEQPYNHC ADLWSLGCIL YQLYYGKPPF CTNHLYKLIN QIVNDPVKFE EPISPDFKSL LKGLLTKSFS ARLNWPHLLN HPFVAFGEED GKWQKTIKEH EMVMKEHMDR LECFRLRAQP SRFQPNSSGH TPRSTGVSRQ SSLGSKESCL FSASSIEIIS SSKNSQEIIE TFKGLATAAD TLTSSPSQST LLLERIPQMG ILGPTLRRIN NGQTIDIVRY ALEFIRILVF PENGSVFPFP SQYPSENIMS ALQSRSEFPP FDFLIRQQVA LELMEKPQEA LDFMMNEFIE NRKNLREDCV KVIFQCVKWG TGFGAALIKM KIFPIFWASL LDSVKESTVR NGSPFQLYAA VAFHTVSALV PHIKLSSPDQ INAHKVSEFV STGLTAVCYY DPSASGDIAA LNCAAAAALL VAFVHREMKD TIVFEPDSIL MDGLCAIVDG VRRISDQLVV SRALGTSYGF ADYGLLDGVT HMLSVVFSDP KSIVYQQNLN SLNRNFLDND KWGSLCTVIG LLRDSDSKVE LSPNGVLAAL RCVQQALQNQ QEGMQSMSLL IESVEPYYGD GGGPISVIAI VCRQLRTKYL KQLRLWPEYR GGGTVGVNAH LTIVVQILLV TFQPAKQGSR SDDDTIAAVQ QVLYKEGVME MLIAALDYTE VAFWGPPFTV IIKLVMGSPL FAKAFVEGDG LHPERIRKVL DSRKASIGLV SDGLNVLSQL ARISRDFYPP IHSANLYESF LDLMRNRDAG IRSKMCNLLG NLCKHSPYFY EPLAKHKLIE ALVERCSDED PTTQKFAAFA AGNAAFHNDF LYEILRPSIP VIVKLLSSSD EKTRQNAAGA VSNFVRNGGS LTSALMGNSV VEALLRILRK DKVGLRKIAL ITIGSFCAYE ECKKKFLWLG LEDALTQLEQ SDAIEAEPSI AKYVTRIKQR IGSS
NT Sequence
atggaaaact atttaatagt tgaactaata ggcgagggat cgtttggcaa ggtttataag gcgagacgga aggggacggg ccaagttgtg gcgatgaagt tcatcgtgaa aaaaggtaaa aatgataagg agctcctaaa tcttcgtagt gaaatagaaa ttatgacaaa gcttgatcat gataatataa ttactttgtt tgaggcgttt gaaacccaac aagagtttgt tgtggtgatg gaatacgccc aaggggagct gtttgaaatt cttgaagacg acaaaaagct tcctgaagac gttgttcaac gtatttcgaa gcaattggtc caggcacttt attatctgca ttctaaccgt attatgcatc gggacatgaa accccagaac attcttattg gccagaatgg ctcagttaaa ttggctgatt ttggttttgc tagatcaatg agttataaca ccatggtgct tacaagtatt aagggaacac ccctttatat ggcaccggaa cttgtccaag agcaaccata caaccactgt gcggatttgt ggtcccttgg atgtattctt taccagttgt actatgggaa accaccattt tgcacaaatc atctttacaa actaattaat caaattgtca atgatcccgt caaatttgaa gaacctatat caccggattt caagtccctt ttaaaaggtc ttttgacaaa gtccttttcg gcgagattga attggcctca cttattgaac catccttttg ttgcttttgg tgaggaggat ggaaagtggc agaagacaat taaagaacac gaaatggtaa tgaaggaaca tatggaccga ttggagtgct ttcgactccg cgctcagcca agcagatttc agcccaactc ttcagggcat acacctcgct ctaccggggt ctcgcgccag tcctccttgg gttccaagga aagttgttta ttttctgctt cttcgataga gattatttct tcttcaaaaa attcacagga aatcattgaa actttcaagg ggcttgcgac agcagctgat acattaacat catctccttc acaaagtact ctcttgctgg aaagaattcc acaaatgggt attttaggac ctacactaag gcggatcaac aatgggcaaa cgatagatat agttcgctat gcattagaat tcattcggat acttgtgttt cctgaaaatg gatctgtttt tccatttcca tcacagtatc cttcggaaaa tataatgagc gccttgcaaa gtagaagtga atttccacca tttgattttc ttatacgaca gcaagtagcc ttggagctca tggaaaaacc ccaggaggca cttgatttta tgatgaacga attcattgag aatcggaaaa atcttcgtga ggattgtgtt aaggtaattt ttcaatgtgt aaaatgggga actgggtttg gagctgcact gatcaaaatg aagattttcc ccattttttg ggcttcgtta cttgattccg taaaagaatc aacagtaaga aatggaagtc cttttcagct ttacgcggca gttgcatttc acacagtttc ggctttggtc ccgcatataa agttgtcttc tcccgaccaa ataaatgcac ataaggtttc tgagtttgtg tctactggat tgacagctgt ttgctactac gacccgtctg caagtggtga tattgctgcg cttaattgtg cagcagcagc agctttattg gtagccttcg tgcaccggga gatgaaggat accattgttt ttgaaccaga ctctatattg atggatggct tgtgtgctat cgtagatggt gttcgtcgaa tttcggatca gcttgtggtg tcacgtgctc ttgggacaag ttatgggttt gctgattatg ggttgttaga cggtgtgacg catatgttgt cggttgtttt ttcggaccca aagtcaatcg tgtatcaaca aaatttgaat tccttaaata gaaattttct tgacaatgac aagtgggggt cactttgtac agtgattggt ctcctccgtg atagcgattc caaggttgaa ttgtcaccga acggcgtgtt ggccgcactt cgttgtgttc agcaggcttt gcagaaccaa caagaaggaa tgcaatcaat gagccttttg atagagtctg tagaacctta ctatggagat ggtggcggcc cgatttccgt aattgcaatt gtttgtcgtc agttaagaac aaaatatctc aagcagcttc gtttgtggcc ggaatataga ggagggggaa ctgtaggggt aaatgcccat cttacaatag ttgttcagat tctgttggtg acgtttcagc cggcaaagca aggttctaga tcggatgatg atactattgc tgctgtacag caagttcttt acaaggaagg ggttatggaa atgcttattg ctgcgcttga ttacacagaa gtagcgtttt ggggtccacc atttacggtc atcataaagc tagttatggg ttcaccatta tttgccaagg cgtttgtaga aggagacggt ttgcatcccg aacggataag gaaagtcctg gattcaagaa aggcgagtat tggtcttgtt tcggacggac ttaacgtgct ttcacaactg gctcgtatat ccagggattt ttatccgcca attcatagcg caaatttgta tgaaagcttt ctagatctta tgcgaaacag agatgcgggt attcggagca agatgtgtaa cctccttgga aatctgtgca aacattcccc atatttttac gaacccctgg caaaacacaa actgatcgag gctcttgtcg agcggtgtag cgatgaggat cccacaacac aaaagtttgc agcctttgcc gccggcaacg cagccttcca caacgatttt ctctatgaaa ttcttaggcc atcgatccca gtcattgtga aactcttgtc tagtagtgat gagaagacaa gacaaaacgc tgccggtgcg gtcagcaatt ttgtgcgaaa tggtggctca ctcacttctg cattaatggg gaacagcgtt gtggaggctc ttctgcggat cctaaggaaa gacaaggtgg ggctaaggaa aatagctctt attactatag gctctttttg tgcatatgag gagtgtaaaa agaagttttt gtggttgggg ttggaggatg ctttaacgca gcttgaacag agcgatgcaa ttgaagctga gccttcaata gcaaagtatg ttacgaggat caagcagcga atcggttcat cgtga
Details for TrcrB.20383.a.B2.GE43853
HARVESTED ON: 8/24/2020
SEQUENCED ON: 8/28/2020
EXPECTED MW: 42kDa
OBSERVED MW: 42kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 98
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHME NYLIVELIGE GSFGKVYKAR RKGTGQVVAM KFIVKKGKND XELLNLRSEI XIMTKLDHDN IITLFEAFET QQEFVVVMEY AQGELFEILE DDKKLPEDVV QRISKQLXQA LYYLHSNRIM HRDMKPQNIL IGQNGSVKLA DFGFARSMSY NTMVLTSIKG TPLYMAPELV QEQPYNHCAD LWSLGCILYQ LYYGKPPFCT NHLYKLINQI VNDPVKFEEP ISPDFKSLLK GLLTKSFSAR LNWPHLLNHP FVAFGEEDGK WQKTIKEHEM VMKEHMDRLE CFRLRAQPSR FQPNSSGHTP RSTGVSRQSS LGSKESCLFS ASSIEIISSS KNSQEIIETF KGLATAADTX T
Validated NT Sequence
atggctcacc accaccacca ccatatggaa aactatttaa tagttgaact aataggcgag ggatcgtttg gcaaggttta taaggcgaga cggaagggga cgggccaagt tgtggcgatg aagttcatcg tgaaaaaagg taaaaatgat aangagctcc taaatcttcg tagtgaaata naaattatga caaagcttga tcatgataat ataattactt tgtttgaggc gtttgaaacc caacaagagt ttgttgtggt gatggaatac gcccaagggg agctgtttga aattcttgaa gacgacaaaa agcttcctga agacgttgtt caacgtattt cgaagcaatt ggnccaggca ctttattatc tgcattctaa ccgtattatg catcgggaca tgaaacccca gaacattctt attggccaga atggctcagt taaattggct gattttggtt ttgctagatc aatgagttat aacaccatgg tgcttacaag tattaaggga acaccccttt atatggcacc ggaacttgtc caagagcaac catacaacca ctgtgcggat ttgtggtccc ttggatgtat tctttaccag ttgtactatg ggaaaccacc attttgcaca aatcatcttt acaaactaat taatcaaatt gtcaatgatc ccgtcaaatt tgaagaacct atatcaccgg atttcaagtc ccttttaaaa ggtcttttga caaagtcctt ttcggcgaga ttgaattggc ctcacttatt gaaccatcct tttgttgctt ttggtgagga ggatggaaag tggcagaaga caattaaaga acacgaaatg gtaatgaagg aacatatgga ccgattggag tgctttcgac tccgcgctca gccaagcaga tttcagccca actcttcagg gcatacacct cgctctaccg gggtctcgcg ccagtcctcc ttgggttcca aggaaagttg tttattttct gcttcttcga tagagattat ttcttcttca aaaaattcac aggaaatcat tgaaactttc aaggggcttg cgacagcagc tgatacattn acntc
Expected Protein Sequence
MAHHHHHHME NYLIVELIGE GSFGKVYKAR RKGTGQVVAM KFIVKKGKND KELLNLRSEI EIMTKLDHDN IITLFEAFET QQEFVVVMEY AQGELFEILE DDKKLPEDVV QRISKQLVQA LYYLHSNRIM HRDMKPQNIL IGQNGSVKLA DFGFARSMSY NTMVLTSIKG TPLYMAPELV QEQPYNHCAD LWSLGCILYQ LYYGKPPFCT NHLYKLINQI VNDPVKFEEP ISPDFKSLLK GLLTKSFSAR LNWPHLLNHP FVAFGEEDGK WQKTIKEHEM VMKEHMDRLE CFRLRAQPSR FQPNSSGHTP RSTGVSRQSS LGSKESCLFS ASSIEIISSS KNSQEIIETF KGLATAADTL TSSPSQST
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggaaa actatttaat agttgaacta ataggcgagg gatcgtttgg caaggtttat aaggcgagac ggaaggggac gggccaagtt gtggcgatga agttcatcgt gaaaaaaggt aaaaatgata aggagctcct aaatcttcgt agtgaaatag aaattatgac aaagcttgat catgataata taattacttt gtttgaggcg tttgaaaccc aacaagagtt tgttgtggtg atggaatacg cccaagggga gctgtttgaa attcttgaag acgacaaaaa gcttcctgaa gacgttgttc aacgtatttc gaagcaattg gtccaggcac tttattatct gcattctaac cgtattatgc atcgggacat gaaaccccag aacattctta ttggccagaa tggctcagtt aaattggctg attttggttt tgctagatca atgagttata acaccatggt gcttacaagt attaagggaa caccccttta tatggcaccg gaacttgtcc aagagcaacc atacaaccac tgtgcggatt tgtggtccct tggatgtatt ctttaccagt tgtactatgg gaaaccacca ttttgcacaa atcatcttta caaactaatt aatcaaattg tcaatgatcc cgtcaaattt gaagaaccta tatcaccgga tttcaagtcc cttttaaaag gtcttttgac aaagtccttt tcggcgagat tgaattggcc tcacttattg aaccatcctt ttgttgcttt tggtgaggag gatggaaagt ggcagaagac aattaaagaa cacgaaatgg taatgaagga acatatggac cgattggagt gctttcgact ccgcgctcag ccaagcagat ttcagcccaa ctcttcaggg catacacctc gctctaccgg ggtctcgcgc cagtcctcct tgggttccaa ggaaagttgt ttattttctg cttcttcgat agagattatt tcttcttcaa aaaattcaca ggaaatcatt gaaactttca aggggcttgc gacagcagct gatacattaa catcatctcc ttcacaaagt acttgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat