SSGCID: Target Page

TrcrB.20202.a

tRNA synthetases class II (A)/Threonyl and Alanyl tRNA synthetase second additional domain containing protein, putative

CENTER ID:	TrcrB.20202.a
ORGANISM:	Trypanosoma cruzi CL Brener
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
TrcrB.20202.a.B1.GE42719	full length		1	462

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

NOTE: Effective as of September 1, 2026, SSGCID will no longer ship physical clone glycerols. SSGCID will provide amino acid and nucleotide sequences for any clones ordered, which the ordering party can then use to order the clone from a third-party clone manufacturer.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	TriTrypDB:TcCLB.506511.30
UniProt:	Q4D4R0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSTAPTFVRV GELACQRNPF LRGLRARVVL CEPVSMNKPA GGSGKGGHNS TNTNPSYNVV LTDSVLFPEG GGQPCDHGTI VYGNEEVPVR NVQRRGDTCV ISTPCPLEAG EEVEVRVDWP RRFDHMQHHS AQHLLSAVVE KAPQCRLPTV SWSLTHPYSF IVLPTGNKID SDVVAHLEKV CNDAIARAVP VRCDMYPSKE AYEQALREKV EKEDSGEIFR QSRSIPSDVT GPIRIITVED GIDQCTCCGT HVSNLTQLQM IKLLHQETKG DTLKLFFVTG DRLCRYYGDM YFREKELMKE LGGVRPEEFV AAAVKRGKEY ADMEKRLKNL TLELMKAESE KLIAEAKASL AEGAGGGIVV YRRDDVGGDF FNALRDAIRQ ACPECLAVLA WGSPVATATA GGALGRAKTG QFMVIGPTDR VESLAPSVCI ALEGKGGMSK YGYRGKGNLA GWNELVQKLR LS

NT Sequence

atgtctactg ccccaacatt tgttcgtgtg ggagaactcg cctgccaacg gaaccccttt cttcgcgggc ttcgcgcccg tgtcgtattg tgtgaacctg tttctatgaa taaacctgcc ggtggcagcg gcaaaggcgg tcataattca accaacacga atccgagtta taatgttgtt ctcacagact ctgtcctctt cccggaggga ggtggtcaac cctgcgatca cggtacgatc gtgtatggga atgaggaggt tcccgttcga aatgtgcagc gtcgcggtga cacgtgtgtg atctcgacac cgtgcccact ggaggcggga gaggaggtgg aggtacgcgt ggactggcca cgacgtttcg atcacatgca gcatcactcc gcgcagcacc tgctctctgc tgttgtggaa aaggcgcccc agtgtcgttt gcccaccgtc tcatggtccc tcactcatcc gtatagtttt attgttctcc cgacaggaaa taaaattgac tcggatgtgg tggcccacct tgaaaaggtt tgcaacgacg ccatcgcacg cgccgtccct gtgcgttgtg acatgtatcc gagcaaggaa gcatatgagc aggcgctaag ggagaaggtg gaaaaggaag attctgggga aatttttcgg cagtcgcgca gcattccatc cgatgtcact ggacccatcc gcatcatcac ggtggaggat ggcattgacc agtgcacatg ttgtggcaca catgtcagca acttgacgca acttcagatg ataaaattgc ttcaccagga gaccaaaggt gacacgctaa aacttttttt tgtcacgggt gatcgattgt gtcgatacta cggtgacatg tactttcgtg agaaggagct aatgaaggag ttaggtgggg tgcgacccga agagtttgtg gctgcagcag tgaaaagggg gaaagaatat gccgacatgg agaagcgact caaaaacttg acgctggagc ttatgaaagc ggaaagtgaa aaacttattg ctgaagcgaa ggcatccttg gcggaaggtg ctggtggtgg cattgttgtg taccgtcgtg atgatgttgg gggagatttc tttaatgctc tacgggatgc aatacgtcag gcgtgtccgg agtgtttggc tgtcttggct tggggcagtc ctgtagcgac ggctacggca ggtggtgctt tggggcgagc gaagactggg cagttcatgg ttattggccc aaccgatcga gtggagtcgt tggccccgtc tgtctgcata gcgctagaag ggaagggcgg gatgtcaaaa tacggctatc gtggcaaagg caaccttgct ggttggaacg aacttgtcca aaaactacgt ttgtcatga

Details for TrcrB.20202.a.B1.GE42719

HARVESTED ON:	9/28/2018
SEQUENCED ON:	10/17/2018
EXPECTED MW:	52kDa
OBSERVED MW:	52kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHMS TAPTFVRVGE LACQRNPFLR GLRARVVLCE PVSMNKPAGG SGKGGHNSTN TNPSYNVVLT DSVLFPEGGG QPCDHGTIVY GNEEVPVRNV QRRGDTCVIS TPCPLEAGEE VEVRVDWPRR FDHMQHHSAQ HLLSAVVEKA PQCRLPTVSW SLTHPYSFIV LPTGNKIDSD VVAHLEKVCN DAIARAVPVR CDMYPSKEAY EQALREKVEK EDSGEIFRQS RSIPSDVTGP IRIITVEDGI DQCTCCGTHV SNLTQLQMIK LLHQETKGDT LKLFFVTGDR LCRYYGDMYF REKELMKELG GVRPEEFVAA AVKRGKEYAD MEKRLKNLTL ELMKAESEKL IAEAKASLAE GAGGGIVVYR RDDVGGDFFN ALRDAIRQAC PECLAVLAWG SPVATATAGG ALGRAKTGQF MVIGPTDRVE SLAPSVCIAL EGKGGMSKYG YRGKGNLAGW NELVQKLRLS

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtcta ctgccccaac atttgttcgt gtgggagaac tcgcctgcca acggaacccc tttcttcgcg ggcttcgcgc ccgtgtcgta ttgtgtgaac ctgtttctat gaataaacct gccggtggca gcggcaaagg cggtcataat tcaaccaaca cgaatccgag ttataatgtt gttctcacag actctgtcct cttcccggag ggaggtggtc aaccctgcga tcacggtacg atcgtgtatg ggaatgagga ggttcccgtt cgaaatgtgc agcgtcgcgg tgacacgtgt gtgatctcga caccgtgccc actggaggcg ggagaggagg tggaggtacg cgtggactgg ccacgacgtt tcgatcacat gcagcatcac tccgcgcagc acctgctctc tgctgttgtg gaaaaggcgc cccagtgtcg tttgcccacc gtctcatggt ccctcactca tccgtatagt tttattgttc tcccgacagg aaataaaatt gactcggatg tggtggccca ccttgaaaag gtttgcaacg acgccatcgc acgcgccgtc cctgtgcgtt gtgacatgta tccgagcaag gaagcatatg agcaggcgct aagggagaag gtggaaaagg aagattctgg ggaaattttt cggcagtcgc gcagcattcc atccgatgtc actggaccca tccgcatcat cacggtggag gatggcattg accagtgcac atgttgtggc acacatgtca gcaacttgac gcaacttcag atgataaaat tgcttcacca ggagaccaaa ggtgacacgc taaaactttt ttttgtcacg ggtgatcgat tgtgtcgata ctacggtgac atgtactttc gtgagaagga gctaatgaag gagttaggtg gggtgcgacc cgaagagttt gtggctgcag cagtgaaaag ggggaaagaa tatgccgaca tggagaagcg actcaaaaac ttgacgctgg agcttatgaa agcggaaagt gaaaaactta ttgctgaagc gaaggcatcc ttggcggaag gtgctggtgg tggcattgtt gtgtaccgtc gtgatgatgt tgggggagat ttctttaatg ctctacggga tgcaatacgt caggcgtgtc cggagtgttt ggctgtcttg gcttggggca gtcctgtagc gacggctacg gcaggtggtg ctttggggcg agcgaagact gggcagttca tggttattgg cccaaccgat cgagtggagt cgttggcccc gtctgtctgc atagcgctag aagggaaggg cgggatgtca aaatacggct atcgtggcaa aggcaacctt gctggttgga acgaacttgt ccaaaaacta cgtttgtcat gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat