TrcrB.19955.b

mitochondrial DNA topoisomerase II, putative

CENTER ID: TrcrB.19955.b
ORGANISM: Trypanosoma cruzi CL Brener
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
TrcrB.19955.b.B1.GE43010 full length 1 1231
TrcrB.19955.b.B2.GE43011 beta domain 1 397
TrcrB.19955.b.B4.GE43012 ordered domain 1 1115
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: TriTrypDB:TcCLB.508277.370
UniProt: Q4E0W1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAEASKYKKL TPIDHVLIRP EMYVGSVDTS SSSMFVFDHE KGRMVWESLK VNHGLLKIVD EILLNASDNI ANKGGRMTYI RVHITEAGEI TIENDGAGIP IVRSKEHKLY IPEMVFGHLL TSSNYDDTSQ NAVAGRHGYG AKLTNILSHR FSVCCRTKGK EFHMSWHDHM RRATAPRVSN VDPRERNLTR VKFLPDYERF GLDANKISHD MKRVLHKRIM DLAAMFPSIE ISLNGVPFAF KSFADYAMLY SSPTSSGEMP PAPFVYESRN GAIAFIPSLT AGTRRIFGVV NGVVTHNGGT HCNAAQEVLQ SSLENVEKAL KKDNKVIDTN RVLRHFTILV FLVQVQPKFD SQNKARLVSV PTMPRVPKQE LMDFLLRMPF LEAHVNTVTG QLAQELNKEM GAGRRMSSKS LISSITKLVD ATTTRRDPRF VRTLIVTEGD SAKALAQNSL SSDQKRYTGV FPLRGKLLNV RNKNLKRLKN CKELQELFCA LGLELGKIYK DAEELRYQRL LVMTDQDADG SHIKGLVINA FEALWPSLLN RNPGFISIFS TPIVKVRLRD KSTHSFFSLK EFHKWQKTHG NVSYTAKYYK GLGTSTTAEG KEYFKDMDKH TMRLVVERND HKLLDSVFDS QEVEWRKDWM TKANAYTGEV DIDRSKKTLT VPDFVHKEMV HFALAGNARA LAHAVDGLKP SQRKILWAIM RRSGNESAKV AQMSGYISEV SAFHHGEMSL QETIIKMAQN FTGGNNINLL IPEGQFGSRQ QLGNDHAAAR YIFTKLSSLA RILFPSEDEP LLDYVTEEGQ QVEPNHYVPI LPLLLCNGSV GIGFGFASNI PPFHPLDVSA AVRSMINGEA AKVVVRRLVP WAVGYQGEVR RGPEGEFIAA GSYQYYVDGR VHVTEIPWTL SIEAFRDHIS VLASKDVVQR IADYSGANHV DIDLELTNGA MTTYAECESE LSLTQRIYIN GTVFSPTGVL TPLEGDLAPV LQWHYDRRLD LYKKRRQRNL GLLEAELARE KSTLKFVTHF REGKIDIVNA TDDSLAKTCS KLGMVRVDDS YDYVLRKPIT FYTKTSLENL NRKIGETEKR IDKLKKTTPV QMWLDELDRF DRAFEEHEKT AVATILKERR VNPPTGDVSR TLQQPRLELE EVKVSSSGGK SVPMRVRVRK YVPPPPSKRP NVGQSVGGGG GGSVRSSAAA VVAHVKAEKK AARARSMQKM LLDVVARQVA RVLPRLPWFL F
NT Sequence
atggcggagg catcgaagta caagaaactc acccccatcg accatgtcct catacggccg gagatgtacg ttggcagcgt ggatacatcc tccagttcca tgtttgtctt tgatcatgag aagggccgca tggtgtggga gtcactgaag gtcaaccacg gcctcctgaa gatcgtggat gaaattctcc tcaacgcctc tgacaacatc gcaaacaaag gcgggcgcat gacgtacatc cgcgttcaca tcacggaggc tggggaaatc acaatagaga atgacggtgc cggtatcccc attgtacgca gcaaagaaca taaactttac atcccagaga tggtttttgg ccaccttctc accagctcca actacgacga cacgagtcag aatgccgtcg caggacgtca cggatacggc gcaaaactaa cgaatatttt gtctcaccgc ttttccgtct gctgccgcac aaaagggaaa gagtttcata tgagttggca cgatcatatg cgcagggcga cagccccacg tgtgtcgaac gtcgacccaa gagagaggaa tctcacacgg gtgaagtttc ttccagacta cgaacgcttt ggattggacg cgaataaaat ctcacacgac atgaagcgtg tgctgcacaa gcgcatcatg gatcttgccg cgatgtttcc aagcattgaa atatcactga acggcgtccc gtttgccttt aaatccttcg ccgactacgc catgttgtac tcttccccca cttccagtgg agaaatgcca ccggcaccgt ttgtgtacga gagccgcaat ggtgccattg cgtttattcc ctcccttacc gccggcacac gtcgtatctt tggtgtcgtg aatggcgtcg tgacgcacaa cggcggcacc cactgcaacg cggcgcagga ggtcttacaa tcctcactgg agaatgttga gaaggcactg aagaaggata acaaagtgat tgacacaaat cgtgttttgc gacattttac gattcttgtg tttctcgtgc aggtgcaacc gaagtttgat tcgcagaaca aggcgcgcct tgtttccgtg ccaacgatgc cgcgtgttcc aaaacaggaa ttgatggact ttcttcttcg catgccgttt ctcgaggcgc atgtcaacac cgtgacgggg cagctcgcac aagaacttaa taaagagatg ggcgccgggc gacgcatgag cagcaaatct ctcatctctt ccatcacgaa attagtggac gccaccacga ctcgccggga cccaaggttc gtccgcaccc tcattgtcac ggagggcgac tctgcaaagg cactcgccca gaactccctg tcaagcgacc agaaacgcta cacaggtgtg ttcccactcc gtgggaagct tttaaacgtg cgcaataaaa atctcaaacg ccttaagaac tgcaaagagc tacaggaact cttctgtgcc ttggggcttg aactggggaa gatctacaag gacgcggagg agctacgcta tcaacgcctg ctagtcatga cagaccagga tgccgatgga tcacacatta aaggacttgt catcaatgcc tttgaggcac tatggccctc gttgctgaat cgcaatccgg gcttcatttc cattttttcg acgcccattg tgaaggtgcg tctgagagac aagtcgaccc attccttttt cagtctgaag gagtttcaca agtggcagaa gacacatgga aatgtttcct ataccgctaa gtattataaa ggtctcggca catccacaac agccgaggga aaggagtact ttaaagacat ggataagcac acaatgcgac ttgtggtgga acgtaatgac cataaattac ttgacagtgt gtttgattcc caggaagtgg agtggcgcaa ggattggatg acaaaggcaa acgcttacac aggggaagtt gatatagatc gaagtaagaa gacactgact gtacccgatt ttgttcacaa agagatggta cactttgcac ttgccggtaa tgcccgcgct cttgcacacg ctgtggatgg attaaaaccc tcccaacgga aaattttgtg ggccattatg cgtcgctctg gtaacgaatc ggccaaagtg gcacagatgt caggttatat ttcggaggtt tcggcctttc accacgggga aatgtcattg caagaaacga ttattaaaat ggctcaaaat tttacgggtg ggaataacat taacctgctc attccagagg gtcagtttgg ttctcgtcag cagctcggta atgatcacgc ggcggctcgt tacatcttta caaaactctc cagtttggca cggatacttt tccccagtga agatgagcct ctgttggatt acgttacgga agaaggacag caggtggagc caaaccacta cgttcccatt cttcctttgc tgctgtgcaa cggcagtgtt ggtattggat ttggttttgc atcaaacatt cctccttttc atccgcttga cgtttcagca gcggtacgaa gcatgattaa tggcgaggct gccaaagttg tagtacggcg gcttgtgccg tgggctgtag ggtatcaggg tgaagtacgt cgagggcccg aaggagaatt cattgcggct ggaagttatc agtactacgt agatggccgt gtgcacgtga cggagatacc atggacactt agcattgagg cattccgcga ccatatttcc gttctcgctt cgaaggatgt tgtgcagcgt attgccgact attcgggtgc gaaccatgtg gacattgact tggaactcac gaatggcgct atgacaacat acgcggagtg tgagagtgaa ctttccctca cccaacgtat ttacatcaat gggaccgtct tctcccccac tggtgtcctc acccctctgg aaggggattt ggcacctgtt ttacagtggc attacgaccg ccgccttgac ctttacaaga aacgacgtca acgtaacttg ggccttcttg aggctgaact cgcacgggag aaatccacgc tgaagtttgt gacccacttt cgtgaaggaa aaattgacat tgtcaacgcc acagacgaca gtctggcaaa gacgtgtagt aaattgggta tggttcgcgt cgatgactcc tatgactatg tcctgcgtaa acccatcacc ttttacacaa aaacgagcct ggagaatctt aaccgcaaaa tcggcgaaac cgagaaacgc atcgacaagc tcaagaagac aacacctgtt cagatgtggc ttgacgagct cgaccgcttc gaccgcgcat ttgaggagca tgagaagacg gcggtggcga ccattcttaa ggagcgacgg gtgaaccccc caacaggaga tgttagtcgg accctgcaac agccgcgact ggagttggag gaggtcaaag tctcttcttc cggtgggaaa agcgtcccaa tgcgggtgag ggtgcggaag tacgtgcccc cgccaccgag taaacggccc aatgtgggcc agagtgttgg tggtggtggt ggtggcagcg tcaggagcag tgcggcggcg gtggtggcac atgtgaaggc cgaaaagaag gcggcgcgag caagaagcat gcagaagatg ttgttagatg tcgttgcacg gcaagtggca cgtgtccttc cacgactgcc gtggtttttg ttttga
Details for TrcrB.19955.b.B1.GE43010
HARVESTED ON: 3/4/2019
SEQUENCED ON: 3/12/2019
EXPECTED MW: 139kDa
OBSERVED MW: 139kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMA EASKYKKLTP IDHVLIRPEM YVGSVDTSSS SMFVFDHEKG RMVWESLKVN HGLLKIVDEI LLNASDNIAN KGGRMTYIRV HITEAGEITI ENDGAGIPIV RSKEHKLYIP EMVFGHLLTS SNYDDTSQNA VAGRHGYGAK LTNILSHRFS VCCRTKGKEF HMSWHDHMRR ATAPRVSNVD PRERNLTRVK FLPDYERFGL DANKISHDMK RVLHKRIMDL AAMFPSIEIS LNGVPFAFKS FADYAMLYSS PTSSGEMPPA PFVYESRNGA IAFIPSLTAG TRRIFGVVNG VVTHNGGTHC NAAQEVLQSS LENVEKALKK DNKVIDTNRV LRHFTILVFL VQVQPKFDSQ NKARLVSVPT MPRVPKQELM DFLLRMPFLE AHVNTVTGQL AQELNKEMGA GRRMSSKSLI SSITKLVDAT TTRRDPRFVR TLIVTEGDSA KALAQNSLSS DQKRYTGVFP LRGKLLNVRN KNLKRLKNCK ELQELFCALG LELGKIYKDA EELRYQRLLV MTDQDADGSH IKGLVINAFE ALWPSLLNRN PGFISIFSTP IVKVRLRDKS THSFFSLKEF HKWQKTHGNV SYTAKYYKGL GTSTTAEGKE YFKDMDKHTM RLVVERNDHK LLDSVFDSQE VEWRKDWMTK ANAYTGEVDI DRSKKTLTVP DFVHKEMVHF ALAGNARALA HAVDGLKPSQ RKILWAIMRR SGNESAKVAQ MSGYISEVSA FHHGEMSLQE TIIKMAQNFT GGNNINLLIP EGQFGSRQQL GNDHAAARYI FTKLSSLARI LFPSEDEPLL DYVTEEGQQV EPNHYVPILP LLLCNGSVGI GFGFASNIPP FHPLDVSAAV RSMINGEAAK VVVRRLVPWA VGYQGEVRRG PEGEFIAAGS YQYYVDGRVH VTEIPWTLSI EAFRDHISVL ASKDVVQRIA DYSGANHVDI DLELTNGAMT TYAECESELS LTQRIYINGT VFSPTGVLTP LEGDLAPVLQ WHYDRRLDLY KKRRQRNLGL LEAELAREKS TLKFVTHFRE GKIDIVNATD DSLAKTCSKL GMVRVDDSYD YVLRKPITFY TKTSLENLNR KIGETEKRID KLKKTTPVQM WLDELDRFDR AFEEHEKTAV ATILKERRVN PPTGDVSRTL QQPRLELEEV KVSSSGGKSV PMRVRVRKYV PPPPSKRPNV GQSVGGGGGG SVRSSAAAVV AHVKAEKKAA RARSMQKMLL DVVARQVARV LPRLPWFLF
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcgg aggcatcgaa gtacaagaaa ctcaccccca tcgaccatgt cctcatacgg ccggagatgt acgttggcag cgtggataca tcctccagtt ccatgtttgt ctttgatcat gagaagggcc gcatggtgtg ggagtcactg aaggtcaacc acggcctcct gaagatcgtg gatgaaattc tcctcaacgc ctctgacaac atcgcaaaca aaggcgggcg catgacgtac atccgcgttc acatcacgga ggctggggaa atcacaatag agaatgacgg tgccggtatc cccattgtac gcagcaaaga acataaactt tacatcccag agatggtttt tggccacctt ctcaccagct ccaactacga cgacacgagt cagaatgccg tcgcaggacg tcacggatac ggcgcaaaac taacgaatat tttgtctcac cgcttttccg tctgctgccg cacaaaaggg aaagagtttc atatgagttg gcacgatcat atgcgcaggg cgacagcccc acgtgtgtcg aacgtcgacc caagagagag gaatctcaca cgggtgaagt ttcttccaga ctacgaacgc tttggattgg acgcgaataa aatctcacac gacatgaagc gtgtgctgca caagcgcatc atggatcttg ccgcgatgtt tccaagcatt gaaatatcac tgaacggcgt cccgtttgcc tttaaatcct tcgccgacta cgccatgttg tactcttccc ccacttccag tggagaaatg ccaccggcac cgtttgtgta cgagagccgc aatggtgcca ttgcgtttat tccctccctt accgccggca cacgtcgtat ctttggtgtc gtgaatggcg tcgtgacgca caacggcggc acccactgca acgcggcgca ggaggtctta caatcctcac tggagaatgt tgagaaggca ctgaagaagg ataacaaagt gattgacaca aatcgtgttt tgcgacattt tacgattctt gtgtttctcg tgcaggtgca accgaagttt gattcgcaga acaaggcgcg ccttgtttcc gtgccaacga tgccgcgtgt tccaaaacag gaattgatgg actttcttct tcgcatgccg tttctcgagg cgcatgtcaa caccgtgacg gggcagctcg cacaagaact taataaagag atgggcgccg ggcgacgcat gagcagcaaa tctctcatct cttccatcac gaaattagtg gacgccacca cgactcgccg ggacccaagg ttcgtccgca ccctcattgt cacggagggc gactctgcaa aggcactcgc ccagaactcc ctgtcaagcg accagaaacg ctacacaggt gtgttcccac tccgtgggaa gcttttaaac gtgcgcaata aaaatctcaa acgccttaag aactgcaaag agctacagga actcttctgt gccttggggc ttgaactggg gaagatctac aaggacgcgg aggagctacg ctatcaacgc ctgctagtca tgacagacca ggatgccgat ggatcacaca ttaaaggact tgtcatcaat gcctttgagg cactatggcc ctcgttgctg aatcgcaatc cgggcttcat ttccattttt tcgacgccca ttgtgaaggt gcgtctgaga gacaagtcga cccattcctt tttcagtctg aaggagtttc acaagtggca gaagacacat ggaaatgttt cctataccgc taagtattat aaaggtctcg gcacatccac aacagccgag ggaaaggagt actttaaaga catggataag cacacaatgc gacttgtggt ggaacgtaat gaccataaat tacttgacag tgtgtttgat tcccaggaag tggagtggcg caaggattgg atgacaaagg caaacgctta cacaggggaa gttgatatag atcgaagtaa gaagacactg actgtacccg attttgttca caaagagatg gtacactttg cacttgccgg taatgcccgc gctcttgcac acgctgtgga tggattaaaa ccctcccaac ggaaaatttt gtgggccatt atgcgtcgct ctggtaacga atcggccaaa gtggcacaga tgtcaggtta tatttcggag gtttcggcct ttcaccacgg ggaaatgtca ttgcaagaaa cgattattaa aatggctcaa aattttacgg gtgggaataa cattaacctg ctcattccag agggtcagtt tggttctcgt cagcagctcg gtaatgatca cgcggcggct cgttacatct ttacaaaact ctccagtttg gcacggatac ttttccccag tgaagatgag cctctgttgg attacgttac ggaagaagga cagcaggtgg agccaaacca ctacgttccc attcttcctt tgctgctgtg caacggcagt gttggtattg gatttggttt tgcatcaaac attcctcctt ttcatccgct tgacgtttca gcagcggtac gaagcatgat taatggcgag gctgccaaag ttgtagtacg gcggcttgtg ccgtgggctg tagggtatca gggtgaagta cgtcgagggc ccgaaggaga attcattgcg gctggaagtt atcagtacta cgtagatggc cgtgtgcacg tgacggagat accatggaca cttagcattg aggcattccg cgaccatatt tccgttctcg cttcgaagga tgttgtgcag cgtattgccg actattcggg tgcgaaccat gtggacattg acttggaact cacgaatggc gctatgacaa catacgcgga gtgtgagagt gaactttccc tcacccaacg tatttacatc aatgggaccg tcttctcccc cactggtgtc ctcacccctc tggaagggga tttggcacct gttttacagt ggcattacga ccgccgcctt gacctttaca agaaacgacg tcaacgtaac ttgggccttc ttgaggctga actcgcacgg gagaaatcca cgctgaagtt tgtgacccac tttcgtgaag gaaaaattga cattgtcaac gccacagacg acagtctggc aaagacgtgt agtaaattgg gtatggttcg cgtcgatgac tcctatgact atgtcctgcg taaacccatc accttttaca caaaaacgag cctggagaat cttaaccgca aaatcggcga aaccgagaaa cgcatcgaca agctcaagaa gacaacacct gttcagatgt ggcttgacga gctcgaccgc ttcgaccgcg catttgagga gcatgagaag acggcggtgg cgaccattct taaggagcga cgggtgaacc ccccaacagg agatgttagt cggaccctgc aacagccgcg actggagttg gaggaggtca aagtctcttc ttccggtggg aaaagcgtcc caatgcgggt gagggtgcgg aagtacgtgc ccccgccacc gagtaaacgg cccaatgtgg gccagagtgt tggtggtggt ggtggtggca gcgtcaggag cagtgcggcg gcggtggtgg cacatgtgaa ggccgaaaag aaggcggcgc gagcaagaag catgcagaag atgttgttag atgtcgttgc acggcaagtg gcacgtgtcc ttccacgact gccgtggttt ttgttttgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t
Details for TrcrB.19955.b.B2.GE43011
HARVESTED ON: 3/4/2019
SEQUENCED ON: 3/12/2019
EXPECTED MW: 46kDa
OBSERVED MW: 46kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMA EASKYKKLTP IDHVLIRPEM YVGSVDTSSS SMFVFDHEKG RMVWESLKVN HGLLKIVDEI LLNASDNIAN KGGRMTYIRV HITEAGEITI ENDGAGIPIV RSKEHKLYIP EMVFGHLLTS SNYDDTSQNA VAGRHGYGAK LTNILSHRFS VCCRTKGKEF HMSWHDHMRR ATAPRVSNVD PRERNLTRVK FLPDYERFGL DANKISHDMK RVLHKRIMDL AAMFPSIEIS LNGVPFAFKS FADYAMLYSS PTSSGEMPPA PFVYESRNGA IAFIPSLTAG TRRIFGVVNG VVTHNGGTHC NAAQEVLQSS LENVEKALKK DNKVIDTNRV LRHFTILVFL VQVQPKFDSQ NKARLVSVPT MPRVPKQELM DFLLRMPFLE AHVNTVTGQL AQELN
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcgg aggcatcgaa gtacaagaaa ctcaccccca tcgaccatgt cctcatacgg ccggagatgt acgttggcag cgtggataca tcctccagtt ccatgtttgt ctttgatcat gagaagggcc gcatggtgtg ggagtcactg aaggtcaacc acggcctcct gaagatcgtg gatgaaattc tcctcaacgc ctctgacaac atcgcaaaca aaggcgggcg catgacgtac atccgcgttc acatcacgga ggctggggaa atcacaatag agaatgacgg tgccggtatc cccattgtac gcagcaaaga acataaactt tacatcccag agatggtttt tggccacctt ctcaccagct ccaactacga cgacacgagt cagaatgccg tcgcaggacg tcacggatac ggcgcaaaac taacgaatat tttgtctcac cgcttttccg tctgctgccg cacaaaaggg aaagagtttc atatgagttg gcacgatcat atgcgcaggg cgacagcccc acgtgtgtcg aacgtcgacc caagagagag gaatctcaca cgggtgaagt ttcttccaga ctacgaacgc tttggattgg acgcgaataa aatctcacac gacatgaagc gtgtgctgca caagcgcatc atggatcttg ccgcgatgtt tccaagcatt gaaatatcac tgaacggcgt cccgtttgcc tttaaatcct tcgccgacta cgccatgttg tactcttccc ccacttccag tggagaaatg ccaccggcac cgtttgtgta cgagagccgc aatggtgcca ttgcgtttat tccctccctt accgccggca cacgtcgtat ctttggtgtc gtgaatggcg tcgtgacgca caacggcggc acccactgca acgcggcgca ggaggtctta caatcctcac tggagaatgt tgagaaggca ctgaagaagg ataacaaagt gattgacaca aatcgtgttt tgcgacattt tacgattctt gtgtttctcg tgcaggtgca accgaagttt gattcgcaga acaaggcgcg ccttgtttcc gtgccaacga tgccgcgtgt tccaaaacag gaattgatgg actttcttct tcgcatgccg tttctcgagg cgcatgtcaa caccgtgacg gggcagctcg cacaagaact taattgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat
Details for TrcrB.19955.b.B4.GE43012
HARVESTED ON: 3/4/2019
SEQUENCED ON: 3/12/2019
EXPECTED MW: 127kDa
OBSERVED MW: 127kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMA EASKYKKLTP IDHVLIRPEM YVGSVDTSSS SMFVFDHEKG RMVWESLKVN HGLLKIVDEI LLNASDNIAN KGGRMTYIRV HITEAGEITI ENDGAGIPIV RSKEHKLYIP EMVFGHLLTS SNYDDTSQNA VAGRHGYGAK LTNILSHRFS VCCRTKGKEF HMSWHDHMRR ATAPRVSNVD PRERNLTRVK FLPDYERFGL DANKISHDMK RVLHKRIMDL AAMFPSIEIS LNGVPFAFKS FADYAMLYSS PTSSGEMPPA PFVYESRNGA IAFIPSLTAG TRRIFGVVNG VVTHNGGTHC NAAQEVLQSS LENVEKALKK DNKVIDTNRV LRHFTILVFL VQVQPKFDSQ NKARLVSVPT MPRVPKQELM DFLLRMPFLE AHVNTVTGQL AQELNKEMGA GRRMSSKSLI SSITKLVDAT TTRRDPRFVR TLIVTEGDSA KALAQNSLSS DQKRYTGVFP LRGKLLNVRN KNLKRLKNCK ELQELFCALG LELGKIYKDA EELRYQRLLV MTDQDADGSH IKGLVINAFE ALWPSLLNRN PGFISIFSTP IVKVRLRDKS THSFFSLKEF HKWQKTHGNV SYTAKYYKGL GTSTTAEGKE YFKDMDKHTM RLVVERNDHK LLDSVFDSQE VEWRKDWMTK ANAYTGEVDI DRSKKTLTVP DFVHKEMVHF ALAGNARALA HAVDGLKPSQ RKILWAIMRR SGNESAKVAQ MSGYISEVSA FHHGEMSLQE TIIKMAQNFT GGNNINLLIP EGQFGSRQQL GNDHAAARYI FTKLSSLARI LFPSEDEPLL DYVTEEGQQV EPNHYVPILP LLLCNGSVGI GFGFASNIPP FHPLDVSAAV RSMINGEAAK VVVRRLVPWA VGYQGEVRRG PEGEFIAAGS YQYYVDGRVH VTEIPWTLSI EAFRDHISVL ASKDVVQRIA DYSGANHVDI DLELTNGAMT TYAECESELS LTQRIYINGT VFSPTGVLTP LEGDLAPVLQ WHYDRRLDLY KKRRQRNLGL LEAELAREKS TLKFVTHFRE GKIDIVNATD DSLAKTCSKL GMVRVDDSYD YVLRKPITFY TKTSLENLNR KIGETEKRID KLKKTTPVQM WLDELDRFDR AFEEHEKTAV ATI
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcgg aggcatcgaa gtacaagaaa ctcaccccca tcgaccatgt cctcatacgg ccggagatgt acgttggcag cgtggataca tcctccagtt ccatgtttgt ctttgatcat gagaagggcc gcatggtgtg ggagtcactg aaggtcaacc acggcctcct gaagatcgtg gatgaaattc tcctcaacgc ctctgacaac atcgcaaaca aaggcgggcg catgacgtac atccgcgttc acatcacgga ggctggggaa atcacaatag agaatgacgg tgccggtatc cccattgtac gcagcaaaga acataaactt tacatcccag agatggtttt tggccacctt ctcaccagct ccaactacga cgacacgagt cagaatgccg tcgcaggacg tcacggatac ggcgcaaaac taacgaatat tttgtctcac cgcttttccg tctgctgccg cacaaaaggg aaagagtttc atatgagttg gcacgatcat atgcgcaggg cgacagcccc acgtgtgtcg aacgtcgacc caagagagag gaatctcaca cgggtgaagt ttcttccaga ctacgaacgc tttggattgg acgcgaataa aatctcacac gacatgaagc gtgtgctgca caagcgcatc atggatcttg ccgcgatgtt tccaagcatt gaaatatcac tgaacggcgt cccgtttgcc tttaaatcct tcgccgacta cgccatgttg tactcttccc ccacttccag tggagaaatg ccaccggcac cgtttgtgta cgagagccgc aatggtgcca ttgcgtttat tccctccctt accgccggca cacgtcgtat ctttggtgtc gtgaatggcg tcgtgacgca caacggcggc acccactgca acgcggcgca ggaggtctta caatcctcac tggagaatgt tgagaaggca ctgaagaagg ataacaaagt gattgacaca aatcgtgttt tgcgacattt tacgattctt gtgtttctcg tgcaggtgca accgaagttt gattcgcaga acaaggcgcg ccttgtttcc gtgccaacga tgccgcgtgt tccaaaacag gaattgatgg actttcttct tcgcatgccg tttctcgagg cgcatgtcaa caccgtgacg gggcagctcg cacaagaact taataaagag atgggcgccg ggcgacgcat gagcagcaaa tctctcatct cttccatcac gaaattagtg gacgccacca cgactcgccg ggacccaagg ttcgtccgca ccctcattgt cacggagggc gactctgcaa aggcactcgc ccagaactcc ctgtcaagcg accagaaacg ctacacaggt gtgttcccac tccgtgggaa gcttttaaac gtgcgcaata aaaatctcaa acgccttaag aactgcaaag agctacagga actcttctgt gccttggggc ttgaactggg gaagatctac aaggacgcgg aggagctacg ctatcaacgc ctgctagtca tgacagacca ggatgccgat ggatcacaca ttaaaggact tgtcatcaat gcctttgagg cactatggcc ctcgttgctg aatcgcaatc cgggcttcat ttccattttt tcgacgccca ttgtgaaggt gcgtctgaga gacaagtcga cccattcctt tttcagtctg aaggagtttc acaagtggca gaagacacat ggaaatgttt cctataccgc taagtattat aaaggtctcg gcacatccac aacagccgag ggaaaggagt actttaaaga catggataag cacacaatgc gacttgtggt ggaacgtaat gaccataaat tacttgacag tgtgtttgat tcccaggaag tggagtggcg caaggattgg atgacaaagg caaacgctta cacaggggaa gttgatatag atcgaagtaa gaagacactg actgtacccg attttgttca caaagagatg gtacactttg cacttgccgg taatgcccgc gctcttgcac acgctgtgga tggattaaaa ccctcccaac ggaaaatttt gtgggccatt atgcgtcgct ctggtaacga atcggccaaa gtggcacaga tgtcaggtta tatttcggag gtttcggcct ttcaccacgg ggaaatgtca ttgcaagaaa cgattattaa aatggctcaa aattttacgg gtgggaataa cattaacctg ctcattccag agggtcagtt tggttctcgt cagcagctcg gtaatgatca cgcggcggct cgttacatct ttacaaaact ctccagtttg gcacggatac ttttccccag tgaagatgag cctctgttgg attacgttac ggaagaagga cagcaggtgg agccaaacca ctacgttccc attcttcctt tgctgctgtg caacggcagt gttggtattg gatttggttt tgcatcaaac attcctcctt ttcatccgct tgacgtttca gcagcggtac gaagcatgat taatggcgag gctgccaaag ttgtagtacg gcggcttgtg ccgtgggctg tagggtatca gggtgaagta cgtcgagggc ccgaaggaga attcattgcg gctggaagtt atcagtacta cgtagatggc cgtgtgcacg tgacggagat accatggaca cttagcattg aggcattccg cgaccatatt tccgttctcg cttcgaagga tgttgtgcag cgtattgccg actattcggg tgcgaaccat gtggacattg acttggaact cacgaatggc gctatgacaa catacgcgga gtgtgagagt gaactttccc tcacccaacg tatttacatc aatgggaccg tcttctcccc cactggtgtc ctcacccctc tggaagggga tttggcacct gttttacagt ggcattacga ccgccgcctt gacctttaca agaaacgacg tcaacgtaac ttgggccttc ttgaggctga actcgcacgg gagaaatcca cgctgaagtt tgtgacccac tttcgtgaag gaaaaattga cattgtcaac gccacagacg acagtctggc aaagacgtgt agtaaattgg gtatggttcg cgtcgatgac tcctatgact atgtcctgcg taaacccatc accttttaca caaaaacgag cctggagaat cttaaccgca aaatcggcga aaccgagaaa cgcatcgaca agctcaagaa gacaacacct gttcagatgt ggcttgacga gctcgaccgc ttcgaccgcg catttgagga gcatgagaag acggcggtgg cgaccatttg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat