TrcrB.19955.a

mitochondrial DNA topoisomerase II, putative

CENTER ID: TrcrB.19955.a
ORGANISM: Trypanosoma cruzi CL Brener
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:
unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
TrcrB.19955.a.B1.GE43006 full length 1 1230
TrcrB.19955.a.B3.GE43008 alpha domain 406 1119
TrcrB.19955.a.B4.GE43009 ordered domain 1 1122
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: TriTrypDB:TcCLB.506445.60
UniProt: Q4DHG5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAEASKYKKL TPIDHVLIRP EMYVGSVDTS SSSMFVFDHE KGRMVWESLK VNHGLLKIVD EILLNASDNI ANKGGRMTYI RVHITEAGEI TIENDGAGIP IVRSKEHKLY IPEMVFGHLL TSSNYDDTSQ NAVAGRHGYG AKLTNILSHR FSVCCRTKGK EFHMSWHDHM RRATAPRVSN VDPREKNLTR VKFLPDYERF GLDANKISHD MKRVLHKRIM DLAAMFPSIE ISLNGVPFAF KSFADYAMLY SSPTSSGEMP PAPFVYESRN GAIAFIPSLT AGTRRIFGVV NGVVTHNGGT HCNAAQEVLQ SSLENVEKAL KKDNKVIDTN RVLRHFTILV FLVQVQPKFD SQNKARLVSV PTMPRVPRQE LMDFLLRMPF LEAHVNTVTG QLADELNKEM GAGRRMSSKS LISSITKLVD ATTTRRDPRF VRTLIVTEGD SAKALAQNSL SSDQKRYTGV FPLRGKLLNV RNKNLKRLKN CKELQELFCA LGLELGKIYK DAEELRYQRL LVMTDQDADG SHIKGLVINA FEALWPSLLN RNPGFISIFS TPIVKVRLRD KSTHSFFSLK EFHKWQKTHG NVSYTAKYYK GLGTSTTAEG KEYFKDMDKH TMRLVVERND HKLLDSVFDS QEVEWRKDWM TKANAYTGEV DIDRSKKTLT VPDFVHKEMV HFALAGNARA LAHAVDGLKP SQRKILWAIM RRSGNESAKV AQLSGYISEV SAFHHGEMSL QETIIKMAQN FTGGNNINLL IPEGQFGSRQ QLGNDHAAAR YIFTKLSSLA RILFPSEDEP LLDYVTEEGQ QVEPNHYVPI LPLLLCNGSV GIGFGFASNI PPFHPLDVSA AVRSMINGEA AKVVVRRLVP WAVGYQGEVR RGPEGEFIAA GSYQYYVDGR VHVTEIPWTL SIEAFRDHIS VLASKDVVQR IADYSGANHV DIDLELTNGA MTTYAECESE LSLTQRIYIN GTVFSPTGVL TPLEGDLAPV LQWHYDRRLD LYKKRRQRNL GLLEAELARE KSTLKFVTHF REGKIDIVNA TDDSLAKTCS KLGMVRVDDS YDYVLRKPIT FYTKTSLENL NRKISETEKR IDKLKKTTPV QMWLDELDRF DRAFEEHEKT AVATILKERR VNPPTGDVSR NLQQPRLELE EVKVSSSGGK SVPMRVRVRK YVPPPPSKRP HVGQSVGGGG GSVRSSAAAV VAHVKAEKKA ARARSMQKML LDVVARQVAR VLPRLPWFLF
NT Sequence
atggcggagg catcgaagta caagaaactc acccccatcg accatgtcct catacggccg gagatgtacg ttggcagcgt ggatacatcc tccagttcca tgtttgtctt tgatcatgag aagggccgca tggtgtggga atcactgaag gtcaaccacg gcctcctgaa gatcgtggat gaaattctcc tcaacgcctc cgacaacatc gcaaacaaag gcgggcgcat gacgtacatc cgcgttcaca tcacggaggc tggggaaatc acaatagaga atgacggtgc cggtatcccc attgtacgca gcaaagaaca taaactttac atcccagaga tggtttttgg ccaccttctc accagctcca actacgacga cacgagtcag aatgccgtcg caggacgtca cgggtacggc gcaaaactaa cgaatatttt gtctcaccgc ttttccgtct gctgccgcac aaaagggaaa gagtttcata tgagttggca cgatcatatg cgcagggcga cagccccacg tgtgtcgaac gtcgacccaa gagagaagaa tctcacacga gtgaagtttc ttccagacta cgaacgcttt ggattggacg cgaataaaat ctcacacgac atgaagcgtg tgctgcacaa gcgcatcatg gatcttgccg cgatgtttcc aagcattgaa atatcactga acggcgtccc gtttgccttt aaatcctttg ctgactacgc catgttgtac tcttccccca cttccagtgg agaaatgcca ccagcaccgt ttgtgtacga gagccgcaat ggtgccattg cgtttattcc ctcccttaca gccggcacac gtcgtatctt tggcgtcgtg aatggcgttg tgacgcacaa cggcggtacc cactgcaacg cggcgcagga ggtcttacaa tcctcactgg agaatgttga gaaggcactg aagaaggata acaaagtgat tgacacaaat cgtgttttgc gacatttcac gattcttgtt tttctcgtgc aggtgcaacc gaagtttgat tcgcagaaca aggcgcgcct tgtttctgtg ccaacgatgc cgcgtgttcc aagacaggaa ttgatggact ttcttcttcg catgccgttt ctcgaggcgc atgtcaacac cgtgacgggg cagctcgcag atgaactgaa taaggagatg ggcgctgggc gacgcatgag cagcaaatct ctcatctctt ccatcacgaa attagtggac gccaccacga ctcgacggga cccaaggttc gtccgcaccc tcattgtcac ggagggcgac tctgcaaagg cactcgccca gaactccctg tcaagcgacc agaaacgcta cacaggtgtg ttcccactcc gtgggaagct tttaaacgtg cgcaataaaa atctcaaacg ccttaaaaac tgcaaagagc tacaggagct cttctgtgcc ttggggcttg aactggggaa gatctacaag gacgcggagg agctacgcta tcaacgcctg ctagtcatga cagaccagga tgccgatgga tcacacatta aaggacttgt catcaatgcc tttgaagcat tatggccctc gttgctgaat cgcaatccgg gctttatttc cattttttcg acgcccattg taaaggtgcg tctgagagac aagtcgaccc attccttttt cagtctgaag gagtttcaca agtggcagaa gacacacgga aatgtttcct acaccgccaa gtattataaa ggtctcggta catccacaac agccgagggg aaggagtact ttaaagacat ggataagcac acaatgcgac ttgtggtgga acgtaatgac cataaattac ttgacagtgt gtttgattcc caggaagtgg agtggcgcaa ggattggatg acaaaggcaa acgcttacac aggggaagtt gatatagatc gaagtaagaa aacattgact gtacccgatt ttgttcacaa agagatggtg cactttgcac ttgccggtaa tgcccgcgcc cttgcacacg ctgtggatgg attaaaaccc tcccaacgaa aaattttgtg ggccattatg cgtcgctctg gtaacgaatc ggccaaagtg gctcagttgt caggttatat ttcggaggtt tcggcctttc accacgggga aatgtcattg caagaaacga ttattaaaat ggctcaaaat tttacgggtg ggaataacat taacctgctc attccagagg gtcagtttgg ttctcgtcag cagctcggta atgatcacgc ggcagctcgt tacatcttta caaaactctc cagtttggca cggatacttt tccccagtga agatgagcct ctgttggatt acgttacgga agaaggacag caggtggagc caaaccacta cgttcccatt cttcctttgc tgctgtgcaa cggcagtgtt ggtattggat ttggttttgc atcaaacatt cctccttttc atccgcttga cgtttcagca gcggtacgaa gcatgattaa tggcgaggct gccaaagttg tagtacggcg gcttgtgccg tgggctgtag ggtatcaggg tgaagtacgt cgagggcccg aaggagaatt cattgcggct ggaagttatc agtactacgt agatggccgt gtgcacgtga cggagatacc atggacactt agcattgagg cattccgcga ccatatttcc gttcttgctt cgaaggatgt tgtgcagcgt attgccgact attcgggtgc gaaccatgtg gacattgact tggaacttac gaatggcgct atgacaacat acgcggagtg tgagagtgaa ctttccctca cccaacgtat ttacatcaat gggactgtct tctcccccac tggtgtcctc acccctctgg aaggggattt ggcacctgtt ttacagtggc attacgaccg ccgccttgac ctttacaaga aacgacgtca acgtaacttg ggccttcttg aggctgaact cgcacgggag aaatccacgc tgaagtttgt gacccacttt cgcgaaggaa aaattgacat tgtcaacgcc acagacgaca gtctggcaaa gacttgtagt aagttgggta tggttcgcgt cgatgattca tatgactatg tcctgcgtaa acccatcacc ttttacacaa aaacgagcct ggagaatctc aaccgcaaaa tcagcgaaac cgagaaacgc attgacaagc tcaagaagac aacacctgtt cagatgtggc ttgacgagct cgaccgcttc gaccgcgcat ttgaggagca tgagaagacg gcggtggcga ccattcttaa ggagcgacgt gtgaaccccc caacaggaga tgttagtcgg aacctgcaac agccgcgact ggagttggag gaggtcaaag tctcttcttc cggagggaaa agcgtcccaa tgcgggtgag ggtgcggaag tacgtgcccc cgccaccgag taaacggccc catgtaggcc agagtgttgg tggtggtggt ggcagcgtca ggagcagtgc ggcggcggtg gtggcacatg tgaaggccga aaagaaagcg gcgcgagcaa gaagcatgca gaagatgttg ttagatgtcg ttgcacggca agtggcacgc gtccttccac gactgccgtg gtttttgttt tga
Details for TrcrB.19955.a.B1.GE43006
HARVESTED ON: 3/4/2019
SEQUENCED ON: 3/12/2019
EXPECTED MW: 139kDa
OBSERVED MW: 139kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMA EASKYKKLTP IDHVLIRPEM YVGSVDTSSS SMFVFDHEKG RMVWESLKVN HGLLKIVDEI LLNASDNIAN KGGRMTYIRV HITEAGEITI ENDGAGIPIV RSKEHKLYIP EMVFGHLLTS SNYDDTSQNA VAGRHGYGAK LTNILSHRFS VCCRTKGKEF HMSWHDHMRR ATAPRVSNVD PREKNLTRVK FLPDYERFGL DANKISHDMK RVLHKRIMDL AAMFPSIEIS LNGVPFAFKS FADYAMLYSS PTSSGEMPPA PFVYESRNGA IAFIPSLTAG TRRIFGVVNG VVTHNGGTHC NAAQEVLQSS LENVEKALKK DNKVIDTNRV LRHFTILVFL VQVQPKFDSQ NKARLVSVPT MPRVPRQELM DFLLRMPFLE AHVNTVTGQL ADELNKEMGA GRRMSSKSLI SSITKLVDAT TTRRDPRFVR TLIVTEGDSA KALAQNSLSS DQKRYTGVFP LRGKLLNVRN KNLKRLKNCK ELQELFCALG LELGKIYKDA EELRYQRLLV MTDQDADGSH IKGLVINAFE ALWPSLLNRN PGFISIFSTP IVKVRLRDKS THSFFSLKEF HKWQKTHGNV SYTAKYYKGL GTSTTAEGKE YFKDMDKHTM RLVVERNDHK LLDSVFDSQE VEWRKDWMTK ANAYTGEVDI DRSKKTLTVP DFVHKEMVHF ALAGNARALA HAVDGLKPSQ RKILWAIMRR SGNESAKVAQ LSGYISEVSA FHHGEMSLQE TIIKMAQNFT GGNNINLLIP EGQFGSRQQL GNDHAAARYI FTKLSSLARI LFPSEDEPLL DYVTEEGQQV EPNHYVPILP LLLCNGSVGI GFGFASNIPP FHPLDVSAAV RSMINGEAAK VVVRRLVPWA VGYQGEVRRG PEGEFIAAGS YQYYVDGRVH VTEIPWTLSI EAFRDHISVL ASKDVVQRIA DYSGANHVDI DLELTNGAMT TYAECESELS LTQRIYINGT VFSPTGVLTP LEGDLAPVLQ WHYDRRLDLY KKRRQRNLGL LEAELAREKS TLKFVTHFRE GKIDIVNATD DSLAKTCSKL GMVRVDDSYD YVLRKPITFY TKTSLENLNR KISETEKRID KLKKTTPVQM WLDELDRFDR AFEEHEKTAV ATILKERRVN PPTGDVSRNL QQPRLELEEV KVSSSGGKSV PMRVRVRKYV PPPPSKRPHV GQSVGGGGGS VRSSAAAVVA HVKAEKKAAR ARSMQKMLLD VVARQVARVL PRLPWFLF
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcgg aggcatcgaa gtacaagaaa ctcaccccca tcgaccatgt cctcatacgg ccggagatgt acgttggcag cgtggataca tcctccagtt ccatgtttgt ctttgatcat gagaagggcc gcatggtgtg ggaatcactg aaggtcaacc acggcctcct gaagatcgtg gatgaaattc tcctcaacgc ctccgacaac atcgcaaaca aaggcgggcg catgacgtac atccgcgttc acatcacgga ggctggggaa atcacaatag agaatgacgg tgccggtatc cccattgtac gcagcaaaga acataaactt tacatcccag agatggtttt tggccacctt ctcaccagct ccaactacga cgacacgagt cagaatgccg tcgcaggacg tcacgggtac ggcgcaaaac taacgaatat tttgtctcac cgcttttccg tctgctgccg cacaaaaggg aaagagtttc atatgagttg gcacgatcat atgcgcaggg cgacagcccc acgtgtgtcg aacgtcgacc caagagagaa gaatctcaca cgagtgaagt ttcttccaga ctacgaacgc tttggattgg acgcgaataa aatctcacac gacatgaagc gtgtgctgca caagcgcatc atggatcttg ccgcgatgtt tccaagcatt gaaatatcac tgaacggcgt cccgtttgcc tttaaatcct ttgctgacta cgccatgttg tactcttccc ccacttccag tggagaaatg ccaccagcac cgtttgtgta cgagagccgc aatggtgcca ttgcgtttat tccctccctt acagccggca cacgtcgtat ctttggcgtc gtgaatggcg ttgtgacgca caacggcggt acccactgca acgcggcgca ggaggtctta caatcctcac tggagaatgt tgagaaggca ctgaagaagg ataacaaagt gattgacaca aatcgtgttt tgcgacattt cacgattctt gtttttctcg tgcaggtgca accgaagttt gattcgcaga acaaggcgcg ccttgtttct gtgccaacga tgccgcgtgt tccaagacag gaattgatgg actttcttct tcgcatgccg tttctcgagg cgcatgtcaa caccgtgacg gggcagctcg cagatgaact gaataaggag atgggcgctg ggcgacgcat gagcagcaaa tctctcatct cttccatcac gaaattagtg gacgccacca cgactcgacg ggacccaagg ttcgtccgca ccctcattgt cacggagggc gactctgcaa aggcactcgc ccagaactcc ctgtcaagcg accagaaacg ctacacaggt gtgttcccac tccgtgggaa gcttttaaac gtgcgcaata aaaatctcaa acgccttaaa aactgcaaag agctacagga gctcttctgt gccttggggc ttgaactggg gaagatctac aaggacgcgg aggagctacg ctatcaacgc ctgctagtca tgacagacca ggatgccgat ggatcacaca ttaaaggact tgtcatcaat gcctttgaag cattatggcc ctcgttgctg aatcgcaatc cgggctttat ttccattttt tcgacgccca ttgtaaaggt gcgtctgaga gacaagtcga cccattcctt tttcagtctg aaggagtttc acaagtggca gaagacacac ggaaatgttt cctacaccgc caagtattat aaaggtctcg gtacatccac aacagccgag gggaaggagt actttaaaga catggataag cacacaatgc gacttgtggt ggaacgtaat gaccataaat tacttgacag tgtgtttgat tcccaggaag tggagtggcg caaggattgg atgacaaagg caaacgctta cacaggggaa gttgatatag atcgaagtaa gaaaacattg actgtacccg attttgttca caaagagatg gtgcactttg cacttgccgg taatgcccgc gcccttgcac acgctgtgga tggattaaaa ccctcccaac gaaaaatttt gtgggccatt atgcgtcgct ctggtaacga atcggccaaa gtggctcagt tgtcaggtta tatttcggag gtttcggcct ttcaccacgg ggaaatgtca ttgcaagaaa cgattattaa aatggctcaa aattttacgg gtgggaataa cattaacctg ctcattccag agggtcagtt tggttctcgt cagcagctcg gtaatgatca cgcggcagct cgttacatct ttacaaaact ctccagtttg gcacggatac ttttccccag tgaagatgag cctctgttgg attacgttac ggaagaagga cagcaggtgg agccaaacca ctacgttccc attcttcctt tgctgctgtg caacggcagt gttggtattg gatttggttt tgcatcaaac attcctcctt ttcatccgct tgacgtttca gcagcggtac gaagcatgat taatggcgag gctgccaaag ttgtagtacg gcggcttgtg ccgtgggctg tagggtatca gggtgaagta cgtcgagggc ccgaaggaga attcattgcg gctggaagtt atcagtacta cgtagatggc cgtgtgcacg tgacggagat accatggaca cttagcattg aggcattccg cgaccatatt tccgttcttg cttcgaagga tgttgtgcag cgtattgccg actattcggg tgcgaaccat gtggacattg acttggaact tacgaatggc gctatgacaa catacgcgga gtgtgagagt gaactttccc tcacccaacg tatttacatc aatgggactg tcttctcccc cactggtgtc ctcacccctc tggaagggga tttggcacct gttttacagt ggcattacga ccgccgcctt gacctttaca agaaacgacg tcaacgtaac ttgggccttc ttgaggctga actcgcacgg gagaaatcca cgctgaagtt tgtgacccac tttcgcgaag gaaaaattga cattgtcaac gccacagacg acagtctggc aaagacttgt agtaagttgg gtatggttcg cgtcgatgat tcatatgact atgtcctgcg taaacccatc accttttaca caaaaacgag cctggagaat ctcaaccgca aaatcagcga aaccgagaaa cgcattgaca agctcaagaa gacaacacct gttcagatgt ggcttgacga gctcgaccgc ttcgaccgcg catttgagga gcatgagaag acggcggtgg cgaccattct taaggagcga cgtgtgaacc ccccaacagg agatgttagt cggaacctgc aacagccgcg actggagttg gaggaggtca aagtctcttc ttccggaggg aaaagcgtcc caatgcgggt gagggtgcgg aagtacgtgc ccccgccacc gagtaaacgg ccccatgtag gccagagtgt tggtggtggt ggtggcagcg tcaggagcag tgcggcggcg gtggtggcac atgtgaaggc cgaaaagaaa gcggcgcgag caagaagcat gcagaagatg ttgttagatg tcgttgcacg gcaagtggca cgcgtccttc cacgactgcc gtggtttttg ttttgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat
Details for TrcrB.19955.a.B3.GE43008
HARVESTED ON: 3/4/2019
SEQUENCED ON: 3/12/2019
EXPECTED MW: 82kDa
OBSERVED MW: 82kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMS SKSLISSITK LVDATTTRRD PRFVRTLIVT EGDSAKALAQ NSLSSDQKRY TGVFPLRGKL LNVRNKNLKR LKNCKELQEL FCALGLELGK IYKDAEELRY QRLLVMTDQD ADGSHIKGLV INAFEALWPS LLNRNPGFIS IFSTPIVKVR LRDKSTHSFF SLKEFHKWQK THGNVSYTAK YYKGLGTSTT AEGKEYFKDM DKHTMRLVVE RNDHKLLDSV FDSQEVEWRK DWMTKANAYT GEVDIDRSKK TLTVPDFVHK EMVHFALAGN ARALAHAVDG LKPSQRKILW AIMRRSGNES AKVAQLSGYI SEVSAFHHGE MSLQETIIKM AQNFTGGNNI NLLIPEGQFG SRQQLGNDHA AARYIFTKLS SLARILFPSE DEPLLDYVTE EGQQVEPNHY VPILPLLLCN GSVGIGFGFA SNIPPFHPLD VSAAVRSMIN GEAAKVVVRR LVPWAVGYQG EVRRGPEGEF IAAGSYQYYV DGRVHVTEIP WTLSIEAFRD HISVLASKDV VQRIADYSGA NHVDIDLELT NGAMTTYAEC ESELSLTQRI YINGTVFSPT GVLTPLEGDL APVLQWHYDR RLDLYKKRRQ RNLGLLEAEL AREKSTLKFV THFREGKIDI VNATDDSLAK TCSKLGMVRV DDSYDYVLRK PITFYTKTSL ENLNRKISET EKRIDKLKKT TPVQMWLDEL DRFDRAFEEH EKTAVATILK ER
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagca gcaaatctct catctcttcc atcacgaaat tagtggacgc caccacgact cgacgggacc caaggttcgt ccgcaccctc attgtcacgg agggcgactc tgcaaaggca ctcgcccaga actccctgtc aagcgaccag aaacgctaca caggtgtgtt cccactccgt gggaagcttt taaacgtgcg caataaaaat ctcaaacgcc ttaaaaactg caaagagcta caggagctct tctgtgcctt ggggcttgaa ctggggaaga tctacaagga cgcggaggag ctacgctatc aacgcctgct agtcatgaca gaccaggatg ccgatggatc acacattaaa ggacttgtca tcaatgcctt tgaagcatta tggccctcgt tgctgaatcg caatccgggc tttatttcca ttttttcgac gcccattgta aaggtgcgtc tgagagacaa gtcgacccat tcctttttca gtctgaagga gtttcacaag tggcagaaga cacacggaaa tgtttcctac accgccaagt attataaagg tctcggtaca tccacaacag ccgaggggaa ggagtacttt aaagacatgg ataagcacac aatgcgactt gtggtggaac gtaatgacca taaattactt gacagtgtgt ttgattccca ggaagtggag tggcgcaagg attggatgac aaaggcaaac gcttacacag gggaagttga tatagatcga agtaagaaaa cattgactgt acccgatttt gttcacaaag agatggtgca ctttgcactt gccggtaatg cccgcgccct tgcacacgct gtggatggat taaaaccctc ccaacgaaaa attttgtggg ccattatgcg tcgctctggt aacgaatcgg ccaaagtggc tcagttgtca ggttatattt cggaggtttc ggcctttcac cacggggaaa tgtcattgca agaaacgatt attaaaatgg ctcaaaattt tacgggtggg aataacatta acctgctcat tccagagggt cagtttggtt ctcgtcagca gctcggtaat gatcacgcgg cagctcgtta catctttaca aaactctcca gtttggcacg gatacttttc cccagtgaag atgagcctct gttggattac gttacggaag aaggacagca ggtggagcca aaccactacg ttcccattct tcctttgctg ctgtgcaacg gcagtgttgg tattggattt ggttttgcat caaacattcc tccttttcat ccgcttgacg tttcagcagc ggtacgaagc atgattaatg gcgaggctgc caaagttgta gtacggcggc ttgtgccgtg ggctgtaggg tatcagggtg aagtacgtcg agggcccgaa ggagaattca ttgcggctgg aagttatcag tactacgtag atggccgtgt gcacgtgacg gagataccat ggacacttag cattgaggca ttccgcgacc atatttccgt tcttgcttcg aaggatgttg tgcagcgtat tgccgactat tcgggtgcga accatgtgga cattgacttg gaacttacga atggcgctat gacaacatac gcggagtgtg agagtgaact ttccctcacc caacgtattt acatcaatgg gactgtcttc tcccccactg gtgtcctcac ccctctggaa ggggatttgg cacctgtttt acagtggcat tacgaccgcc gccttgacct ttacaagaaa cgacgtcaac gtaacttggg ccttcttgag gctgaactcg cacgggagaa atccacgctg aagtttgtga cccactttcg cgaaggaaaa attgacattg tcaacgccac agacgacagt ctggcaaaga cttgtagtaa gttgggtatg gttcgcgtcg atgattcata tgactatgtc ctgcgtaaac ccatcacctt ttacacaaaa acgagcctgg agaatctcaa ccgcaaaatc agcgaaaccg agaaacgcat tgacaagctc aagaagacaa cacctgttca gatgtggctt gacgagctcg accgcttcga ccgcgcattt gaggagcatg agaagacggc ggtggcgacc attcttaagg agcgatgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat
Details for TrcrB.19955.a.B4.GE43009
HARVESTED ON: data unavailable
SEQUENCED ON: 3/12/2019
EXPECTED MW: 128kDa
OBSERVED MW: 82kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMA EASKYKKLTP IDHVLIRPEM YVGSVDTSSS SMFVFDHEKG RMVWESLKVN HGLLKIVDEI LLNASDNIAN KGGRMTYIRV HITEAGEITI ENDGAGIPIV RSKEHKLYIP EMVFGHLLTS SNYDDTSQNA VAGRHGYGAK LTNILSHRFS VCCRTKGKEF HMSWHDHMRR ATAPRVSNVD PREKNLTRVK FLPDYERFGL DANKISHDMK RVLHKRIMDL AAMFPSIEIS LNGVPFAFKS FADYAMLYSS PTSSGEMPPA PFVYESRNGA IAFIPSLTAG TRRIFGVVNG VVTHNGGTHC NAAQEVLQSS LENVEKALKK DNKVIDTNRV LRHFTILVFL VQVQPKFDSQ NKARLVSVPT MPRVPRQELM DFLLRMPFLE AHVNTVTGQL ADELNKEMGA GRRMSSKSLI SSITKLVDAT TTRRDPRFVR TLIVTEGDSA KALAQNSLSS DQKRYTGVFP LRGKLLNVRN KNLKRLKNCK ELQELFCALG LELGKIYKDA EELRYQRLLV MTDQDADGSH IKGLVINAFE ALWPSLLNRN PGFISIFSTP IVKVRLRDKS THSFFSLKEF HKWQKTHGNV SYTAKYYKGL GTSTTAEGKE YFKDMDKHTM RLVVERNDHK LLDSVFDSQE VEWRKDWMTK ANAYTGEVDI DRSKKTLTVP DFVHKEMVHF ALAGNARALA HAVDGLKPSQ RKILWAIMRR SGNESAKVAQ LSGYISEVSA FHHGEMSLQE TIIKMAQNFT GGNNINLLIP EGQFGSRQQL GNDHAAARYI FTKLSSLARI LFPSEDEPLL DYVTEEGQQV EPNHYVPILP LLLCNGSVGI GFGFASNIPP FHPLDVSAAV RSMINGEAAK VVVRRLVPWA VGYQGEVRRG PEGEFIAAGS YQYYVDGRVH VTEIPWTLSI EAFRDHISVL ASKDVVQRIA DYSGANHVDI DLELTNGAMT TYAECESELS LTQRIYINGT VFSPTGVLTP LEGDLAPVLQ WHYDRRLDLY KKRRQRNLGL LEAELAREKS TLKFVTHFRE GKIDIVNATD DSLAKTCSKL GMVRVDDSYD YVLRKPITFY TKTSLENLNR KISETEKRID KLKKTTPVQM WLDELDRFDR AFEEHEKTAV ATILKERRVN
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcgg aggcatcgaa gtacaagaaa ctcaccccca tcgaccatgt cctcatacgg ccggagatgt acgttggcag cgtggataca tcctccagtt ccatgtttgt ctttgatcat gagaagggcc gcatggtgtg ggaatcactg aaggtcaacc acggcctcct gaagatcgtg gatgaaattc tcctcaacgc ctccgacaac atcgcaaaca aaggcgggcg catgacgtac atccgcgttc acatcacgga ggctggggaa atcacaatag agaatgacgg tgccggtatc cccattgtac gcagcaaaga acataaactt tacatcccag agatggtttt tggccacctt ctcaccagct ccaactacga cgacacgagt cagaatgccg tcgcaggacg tcacgggtac ggcgcaaaac taacgaatat tttgtctcac cgcttttccg tctgctgccg cacaaaaggg aaagagtttc atatgagttg gcacgatcat atgcgcaggg cgacagcccc acgtgtgtcg aacgtcgacc caagagagaa gaatctcaca cgagtgaagt ttcttccaga ctacgaacgc tttggattgg acgcgaataa aatctcacac gacatgaagc gtgtgctgca caagcgcatc atggatcttg ccgcgatgtt tccaagcatt gaaatatcac tgaacggcgt cccgtttgcc tttaaatcct ttgctgacta cgccatgttg tactcttccc ccacttccag tggagaaatg ccaccagcac cgtttgtgta cgagagccgc aatggtgcca ttgcgtttat tccctccctt acagccggca cacgtcgtat ctttggcgtc gtgaatggcg ttgtgacgca caacggcggt acccactgca acgcggcgca ggaggtctta caatcctcac tggagaatgt tgagaaggca ctgaagaagg ataacaaagt gattgacaca aatcgtgttt tgcgacattt cacgattctt gtttttctcg tgcaggtgca accgaagttt gattcgcaga acaaggcgcg ccttgtttct gtgccaacga tgccgcgtgt tccaagacag gaattgatgg actttcttct tcgcatgccg tttctcgagg cgcatgtcaa caccgtgacg gggcagctcg cagatgaact gaataaggag atgggcgctg ggcgacgcat gagcagcaaa tctctcatct cttccatcac gaaattagtg gacgccacca cgactcgacg ggacccaagg ttcgtccgca ccctcattgt cacggagggc gactctgcaa aggcactcgc ccagaactcc ctgtcaagcg accagaaacg ctacacaggt gtgttcccac tccgtgggaa gcttttaaac gtgcgcaata aaaatctcaa acgccttaaa aactgcaaag agctacagga gctcttctgt gccttggggc ttgaactggg gaagatctac aaggacgcgg aggagctacg ctatcaacgc ctgctagtca tgacagacca ggatgccgat ggatcacaca ttaaaggact tgtcatcaat gcctttgaag cattatggcc ctcgttgctg aatcgcaatc cgggctttat ttccattttt tcgacgccca ttgtaaaggt gcgtctgaga gacaagtcga cccattcctt tttcagtctg aaggagtttc acaagtggca gaagacacac ggaaatgttt cctacaccgc caagtattat aaaggtctcg gtacatccac aacagccgag gggaaggagt actttaaaga catggataag cacacaatgc gacttgtggt ggaacgtaat gaccataaat tacttgacag tgtgtttgat tcccaggaag tggagtggcg caaggattgg atgacaaagg caaacgctta cacaggggaa gttgatatag atcgaagtaa gaaaacattg actgtacccg attttgttca caaagagatg gtgcactttg cacttgccgg taatgcccgc gcccttgcac acgctgtgga tggattaaaa ccctcccaac gaaaaatttt gtgggccatt atgcgtcgct ctggtaacga atcggccaaa gtggctcagt tgtcaggtta tatttcggag gtttcggcct ttcaccacgg ggaaatgtca ttgcaagaaa cgattattaa aatggctcaa aattttacgg gtgggaataa cattaacctg ctcattccag agggtcagtt tggttctcgt cagcagctcg gtaatgatca cgcggcagct cgttacatct ttacaaaact ctccagtttg gcacggatac ttttccccag tgaagatgag cctctgttgg attacgttac ggaagaagga cagcaggtgg agccaaacca ctacgttccc attcttcctt tgctgctgtg caacggcagt gttggtattg gatttggttt tgcatcaaac attcctcctt ttcatccgct tgacgtttca gcagcggtac gaagcatgat taatggcgag gctgccaaag ttgtagtacg gcggcttgtg ccgtgggctg tagggtatca gggtgaagta cgtcgagggc ccgaaggaga attcattgcg gctggaagtt atcagtacta cgtagatggc cgtgtgcacg tgacggagat accatggaca cttagcattg aggcattccg cgaccatatt tccgttcttg cttcgaagga tgttgtgcag cgtattgccg actattcggg tgcgaaccat gtggacattg acttggaact tacgaatggc gctatgacaa catacgcgga gtgtgagagt gaactttccc tcacccaacg tatttacatc aatgggactg tcttctcccc cactggtgtc ctcacccctc tggaagggga tttggcacct gttttacagt ggcattacga ccgccgcctt gacctttaca agaaacgacg tcaacgtaac ttgggccttc ttgaggctga actcgcacgg gagaaatcca cgctgaagtt tgtgacccac tttcgcgaag gaaaaattga cattgtcaac gccacagacg acagtctggc aaagacttgt agtaagttgg gtatggttcg cgtcgatgat tcatatgact atgtcctgcg taaacccatc accttttaca caaaaacgag cctggagaat ctcaaccgca aaatcagcga aaccgagaaa cgcattgaca agctcaagaa gacaacacct gttcagatgt ggcttgacga gctcgaccgc ttcgaccgcg catttgagga gcatgagaag acggcggtgg cgaccattct taaggagcga cgtgtgaact gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat