TrcrB.19530.b

J-binding protein 1 (JBP1)

CENTER ID:	TrcrB.19530.b
ORGANISM:	Trypanosoma cruzi CL Brener
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
TrcrB.19530.b.B1.GE41525	full length		1	832
TrcrB.19530.b.B2.GE41526	C-term domain		585	827

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Molecular models

METHOD	RESULTS
comparative modelling	Robetta_79413

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	TriTrypDB:TcCLB.510357.10
UniProt:	Q4DBW3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MKQKRGKQDV KMLESAPPQL LPKKGRLEIS ELAPQQRTIR TAEEIETAYN EAVRKHPFYD NADHTIDFHD ATVFRDARGV VGGVFLPGAL PAFAATMAAD VLRPAAVRTS LRSNMFGGFA PLSGIAGYFD YRGSPVELKC RKTSFTYENV HSWPNVFPMI DYVSAIYKAV FPEQWAAQDA AVPDIVRIHG SPFSTLTVNQ QFRTASHTDA GDFDMGYGLL AVLEGKFEGL SLALDDFGVC FRMQPRDILI FNTHFFHSNT EPELNHPRDD WSRLTCVCYY RAALGEPACV AEYERRLARA KEIGASPPPA VDAILQKDNG NNFNKPAPTF PYLLTPFGGA ASVCSLHCCT AKLLRLHELL LENPTLEVIL FGESLRTDDG LPRREKEQLI SVHLPVVVKM SPSGGFSELG GALKAAEEKQ YFFEEKYLAD ELGPDLMSMW TQSRAHWLRL VKEDWERLCR RDPERTKFTW NNSSAMNAAF FDLCEVAKQM MIGLLNKETP SSAENHSFWI LFAAHLNYAC TTENGMPRDA VGMHKLNVKL KDFHFGGTRY LKDMPPEEQE RRLERKKRIE EARRRGSSAH ETHTDNWLLN DTFDYQQEDR KVEFEENGWM TPEAYVKHLG LKPCGDVTAA ASPTEPIHVL VVLPRPAAAA TAKDAKRDVP LATSEESIRL LMNPAAQRVL RGKARNVALP SPLSFGGVKI TVLFDGDDID CIHPDFVILQ HLLATIEEDE AAKARVKYWA RVARYCVFVV ETDVRDRRHF LLREEVRVAY EDVAEDCFRS LHAAAYSTKY NRLRTTPSLI ALCNRKNIGL RFKFRGSPLN TIALVVVGER LD

NT Sequence

atgaagcaaa agagaggaaa gcaagacgtg aaaatgcttg aatcagctcc tccacaactt ctcccaaaaa agggtcggct tgagatttct gagcttgctc cacaacaacg gacaatacgt actgcggaag aaattgaaac ggcatacaat gaggccgtta gaaaacatcc tttttacgat aacgccgatc acacgattga ttttcatgac gccactgtat tccgtgacgc cagaggggtg gtcgggggcg tctttttacc aggggcgctg cctgcatttg ccgccaccat ggcagccgat gtgttgcgac ctgcagctgt aaggacatct ttacgatcga atatgtttgg tgggttcgca cctcttagtg gtatcgccgg ttactttgac tatcgcggat ctccagtgga actcaaatgc cgcaagacat cctttacata tgaaaacgtt cattcctggc caaatgtgtt tccgatgatt gattacgtca gtgccattta caaagctgtt ttcccggaac agtgggccgc gcaggatgca gccgtgccgg atattgtccg cattcacgga tccccctttt ccaccttgac ggtgaatcaa cagtttcgga ccgcctcaca cacggatgcg ggtgattttg atatgggtta cggtcttctg gcggttctgg agggcaaatt tgaaggtctc tccctcgcgt tggatgactt tggtgtctgt ttccgtatgc agccaaggga catcttgatt tttaatacgc acttctttca ttcgaataca gagccggagt tgaatcatcc aagggacgac tggagtcgac taacctgtgt ttgctattat agggcggcgt tgggggaacc ggcgtgtgtt gcagaatatg aacgacgttt ggcgcgggct aaagaaatag gtgcctcccc ccctcctgct gtagatgcca ttttacaaaa ggacaatggc aacaatttta acaaacccgc accgacgttc ccttatttac tcacaccgtt tggaggagcg gcgagcgttt gctctctgca ttgctgcacc gccaagctcc tgcggctaca cgagctactg ttagaaaacc caacgttgga agttatttta tttggagaat cgctacgaac ggacgatggc ctgcccagac gtgaaaagga gcaacttatt tctgtacacc ttcctgtggt tgtaaagatg tctccctccg gtgggtttag tgaattggga ggggcgctaa aggctgccga ggagaagcag tacttctttg aggaaaaata cttggcggat gaactcggtc cagacctcat gtccatgtgg acgcagagtc gtgctcactg gctgaggctc gtcaaggagg attgggagcg tctctgtcga agggatccag aacggacaaa atttacctgg aataattcgt ctgcaatgaa tgcggccttt tttgatctct gtgaggtggc aaagcagatg atgattggac tgctaaacaa ggaaacacca tcatctgctg agaaccactc cttttggatt ctttttgcgg cacatttaaa ttacgcatgc accactgaaa atggcatgcc gcgtgatgcc gttgggatgc ataaattaaa tgttaagctg aaggattttc actttggtgg aacccggtac ttgaaggata tgccacctga ggagcaggag aggcgcttgg aaaggaagaa gcgtattgag gaggcacgcc gtcgaggtag ctcagcgcat gaaacgcaca cggataattg gttgctaaat gacacgtttg attatcagca agaggacaga aaggtggaat ttgaggaaaa cggctggatg actccagagg cgtatgtaaa gcatctgggt ctcaaaccat gtggcgatgt cacggcggct gcaagtccca ccgaacctat tcatgttctt gtcgtcttgc caagaccggc ggcggcggcg acggcaaaag atgccaagcg ggatgttcct ttggctacat ctgaagaatc catccgtctt ttaatgaatc cggccgcgca gcgtgtgctg agaggaaagg cccggaatgt cgcccttcct tccccattgt cgtttggtgg tgtgaaaatc acagttcttt ttgatggtga tgatattgac tgtattcatc ccgattttgt gattctgcaa catctccttg ccaccattga agaggatgaa gcggcgaagg cacgtgtgaa gtattgggcc cgcgtggcac gctattgcgt gtttgtcgtg gagacggatg tcagggaccg acgtcatttt ctcctccgtg aggaagtacg ggtcgcctac gaggatgttg ccgaggactg ctttagatcc cttcatgcgg cggcgtactc aacaaaatac aatcgtttga gaacgacgcc ttctcttatc gctctctgta atagaaaaaa tattgggctc cgctttaagt tcaggggttc acccctaaat acaatagccc tcgtcgtggt cggggagcgg ttagattga

Details for TrcrB.19530.b.B1.GE41525

HARVESTED ON:	4/21/2017
SEQUENCED ON:	5/3/2017
EXPECTED MW:	95kDa
OBSERVED MW:	95kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHMK QKRGKQDVKM LESAPPQLLP KKGRLEISEL APQQRTIRTA EEIETAYNEA VRKHPFYDNA DHTIDFHDAT VFRDARGVVG GVFLPGALPA FAATMAADVL RPAAVRTSLR SNMFGGFAPL SGIAGYFDYR GSPVELKCRK TSFTYENVHS WPNVFPMIDY VSAIYKAVFP EQWAAQDAAV PDIVRIHGSP FSTLTVNQQF RTASHTDAGD FDMGYGLLAV LEGKFEGLSL ALDDFGVCFR MQPRDILIFN THFFHSNTEP ELNHPRDDWS RLTCVCYYRA ALGEPACVAE YERRLARAKE IGASPPPAVD AILQKDNGNN FNKPAPTFPY LLTPFGGAAS VCSLHCCTAK LLRLHELLLE NPTLEVILFG ESLRTDDGLP RREKEQLISV HLPVVVKMSP SGGFSELGGA LKAAEEKQYF FEEKYLADEL GPDLMSMWTQ SRAHWLRLVK EDWERLCRRD PERTKFTWNN SSAMNAAFFD LCEVAKQMMI GLLNKETPSS AENHSFWILF AAHLNYACTT ENGMPRDAVG MHKLNVKLKD FHFGGTRYLK DMPPEEQERR LERKKRIEEA RRRGSSAHET HTDNWLLNDT FDYQQEDRKV EFEENGWMTP EAYVKHLGLK PCGDVTAAAS PTEPIHVLVV LPRPAAAATA KDAKRDVPLA TSEESIRLLM NPAAQRVLRG KARNVALPSP LSFGGVKITV LFDGDDIDCI HPDFVILQHL LATIEEDEAA KARVKYWARV ARYCVFVVET DVRDRRHFLL REEVRVAYED VAEDCFRSLH AAAYSTKYNR LRTTPSLIAL CNRKNIGLRF KFRGSPLNTI ALVVVGERLD

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaagc aaaagagagg aaagcaagac gtgaaaatgc ttgaatcagc tcctccacaa cttctcccaa aaaagggtcg gcttgagatt tctgagcttg ctccacaaca acggacaata cgtactgcgg aagaaattga aacggcatac aatgaggccg ttagaaaaca tcctttttac gataacgccg atcacacgat tgattttcat gacgccactg tattccgtga cgccagaggg gtggtcgggg gcgtcttttt accaggggcg ctgcctgcat ttgccgccac catggcagcc gatgtgttgc gacctgcagc tgtaaggaca tctttacgat cgaatatgtt tggtgggttc gcacctctta gtggtatcgc cggttacttt gactatcgcg gatctccagt ggaactcaaa tgccgcaaga catcctttac atatgaaaac gttcattcct ggccaaatgt gtttccgatg attgattacg tcagtgccat ttacaaagct gttttcccgg aacagtgggc cgcgcaggat gcagccgtgc cggatattgt ccgcattcac ggatccccct tttccacctt gacggtgaat caacagtttc ggaccgcctc acacacggat gcgggtgatt ttgatatggg ttacggtctt ctggcggttc tggagggcaa atttgaaggt ctctccctcg cgttggatga ctttggtgtc tgtttccgta tgcagccaag ggacatcttg atttttaata cgcacttctt tcattcgaat acagagccgg agttgaatca tccaagggac gactggagtc gactaacctg tgtttgctat tatagggcgg cgttggggga accggcgtgt gttgcagaat atgaacgacg tttggcgcgg gctaaagaaa taggtgcctc cccccctcct gctgtagatg ccattttaca aaaggacaat ggcaacaatt ttaacaaacc cgcaccgacg ttcccttatt tactcacacc gtttggagga gcggcgagcg tttgctctct gcattgctgc accgccaagc tcctgcggct acacgagcta ctgttagaaa acccaacgtt ggaagttatt ttatttggag aatcgctacg aacggacgat ggcctgccca gacgtgaaaa ggagcaactt atttctgtac accttcctgt ggttgtaaag atgtctccct ccggtgggtt tagtgaattg ggaggggcgc taaaggctgc cgaggagaag cagtacttct ttgaggaaaa atacttggcg gatgaactcg gtccagacct catgtccatg tggacgcaga gtcgtgctca ctggctgagg ctcgtcaagg aggattggga gcgtctctgt cgaagggatc cagaacggac aaaatttacc tggaataatt cgtctgcaat gaatgcggcc ttttttgatc tctgtgaggt ggcaaagcag atgatgattg gactgctaaa caaggaaaca ccatcatctg ctgagaacca ctccttttgg attctttttg cggcacattt aaattacgca tgcaccactg aaaatggcat gccgcgtgat gccgttggga tgcataaatt aaatgttaag ctgaaggatt ttcactttgg tggaacccgg tacttgaagg atatgccacc tgaggagcag gagaggcgct tggaaaggaa gaagcgtatt gaggaggcac gccgtcgagg tagctcagcg catgaaacgc acacggataa ttggttgcta aatgacacgt ttgattatca gcaagaggac agaaaggtgg aatttgagga aaacggctgg atgactccag aggcgtatgt aaagcatctg ggtctcaaac catgtggcga tgtcacggcg gctgcaagtc ccaccgaacc tattcatgtt cttgtcgtct tgccaagacc ggcggcggcg gcgacggcaa aagatgccaa gcgggatgtt cctttggcta catctgaaga atccatccgt cttttaatga atccggccgc gcagcgtgtg ctgagaggaa aggcccggaa tgtcgccctt ccttccccat tgtcgtttgg tggtgtgaaa atcacagttc tttttgatgg tgatgatatt gactgtattc atcccgattt tgtgattctg caacatctcc ttgccaccat tgaagaggat gaagcggcga aggcacgtgt gaagtattgg gcccgcgtgg cacgctattg cgtgtttgtc gtggagacgg atgtcaggga ccgacgtcat tttctcctcc gtgaggaagt acgggtcgcc tacgaggatg ttgccgagga ctgctttaga tcccttcatg cggcggcgta ctcaacaaaa tacaatcgtt tgagaacgac gccttctctt atcgctctct gtaatagaaa aaatattggg ctccgcttta agttcagggg ttcaccccta aatacaatag ccctcgtcgt ggtcggggag cggttagatt gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat

Details for TrcrB.19530.b.B2.GE41526

HARVESTED ON:	4/21/2017
SEQUENCED ON:	5/3/2017
EXPECTED MW:	28kDa
OBSERVED MW:	28kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMD NWLLNDTFDY QQEDRKVEFE ENGWMTPEAY VKHLGLKPCG DVTAAASPTE PIHVLVVLPR PAAAATAKDA KRDVPLATSE ESIRLLMNPA AQRVLRGKAR NVALPSPLSF GGVKITVLFD GDDIDCIHPD FVILQHLLAT IEEDEAAKAR VKYWARVARY CVFVVETDVR DRRHFLLREE VRVAYEDVAE DCFRSLHAAA YSTKYNRLRT TPSLIALCNR KNIGLRFKFR GSPLNTIALV VV

Validated NT Sequence

atggctcacc accaccacca ccatatggat aattggttgc taaatgacac gtttgattat cagcaagagg acagaaaggt ggaatttgag gaaaacggct ggatgactcc agaggcgtat gtaaagcatc tgggtctcaa accatgtggc gatgtcacgg cggctgcaag tcccaccgaa cctattcatg ttcttgtcgt cttgccaaga ccggcggcgg cggcgacggc aaaagatgcc aagcgggatg ttcctttggc tacatctgaa gaatccatcc gtcttttaat gaatccggcc gcgcagcgtg tgctgagagg aaaggcccgg aatgtcgccc ttccttcccc attgtcgttt ggtggtgtga aaatcacagt tctttttgat ggtgatgata ttgactgtat tcatcccgat tttgtgattc tgcaacatct ccttgccacc attgaagagg atgaagcggc gaaggcacgt gtgaagtatt gggcccgcgt ggcacgctat tgcgtgtttg tcgtggagac ggatgtcagg gaccgacgtc attttctcct ccgtgaggaa gtacgggtcg cctacgagga tgttgccgag gactgcttta gatcccttca tgcggcggcg tactcaacaa aatacaatcg tttgagaacg acgccttctc ttatcgctct ctgtaataga aaaaatattg ggctccgctt taagttcagg ggttcacccc taaatacaat agccctcgtc gtggtctaag tgagtaagat

Expected Protein Sequence

MAHHHHHHDN WLLNDTFDYQ QEDRKVEFEE NGWMTPEAYV KHLGLKPCGD VTAAASPTEP IHVLVVLPRP AAAATAKDAK RDVPLATSEE SIRLLMNPAA QRVLRGKARN VALPSPLSFG GVKITVLFDG DDIDCIHPDF VILQHLLATI EEDEAAKARV KYWARVARYC VFVVETDVRD RRHFLLREEV RVAYEDVAED CFRSLHAAAY STKYNRLRTT PSLIALCNRK NIGLRFKFRG SPLNTIALVV V

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgataatt ggttgctaaa tgacacgttt gattatcagc aagaggacag aaaggtggaa tttgaggaaa acggctggat gactccagag gcgtatgtaa agcatctggg tctcaaacca tgtggcgatg tcacggcggc tgcaagtccc accgaaccta ttcatgttct tgtcgtcttg ccaagaccgg cggcggcggc gacggcaaaa gatgccaagc gggatgttcc tttggctaca tctgaagaat ccatccgtct tttaatgaat ccggccgcgc agcgtgtgct gagaggaaag gcccggaatg tcgcccttcc ttccccattg tcgtttggtg gtgtgaaaat cacagttctt tttgatggtg atgatattga ctgtattcat cccgattttg tgattctgca acatctcctt gccaccattg aagaggatga agcggcgaag gcacgtgtga agtattgggc ccgcgtggca cgctattgcg tgtttgtcgt ggagacggat gtcagggacc gacgtcattt tctcctccgt gaggaagtac gggtcgccta cgaggatgtt gccgaggact gctttagatc ccttcatgcg gcggcgtact caacaaaata caatcgtttg agaacgacgc cttctcttat cgctctctgt aatagaaaaa atattgggct ccgctttaag ttcaggggtt cacccctaaa tacaatagcc ctcgtcgtgg tctgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat